Academic Journal
Deubiquitinase USP7 Regulates Neutrophil Extracellular Trap Formation and Inflammation in Lipopolysaccharide-Treated Mice Through ICAM-1 Expression.
| Τίτλος: | Deubiquitinase USP7 Regulates Neutrophil Extracellular Trap Formation and Inflammation in Lipopolysaccharide-Treated Mice Through ICAM-1 Expression. |
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| Συγγραφείς: | Xu H; Department of Emergency, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China., Wang Q; Department of Emergency, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China., Fan JX; Department of Emergency, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China., Xu YF; Department of Emergency, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China., Yu J; Department of Emergency, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China. |
| Πηγή: | The Kaohsiung journal of medical sciences [Kaohsiung J Med Sci] 2026 May; Vol. 42 (5), pp. e70133. Date of Electronic Publication: 2025 Oct 31. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Department of Public Health, Kaohsiung Medical University Country of Publication: China (Republic : 1949- ) NLM ID: 100960562 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2410-8650 (Electronic) Linking ISSN: 1607551X NLM ISO Abbreviation: Kaohsiung J Med Sci Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2019- : [Kaohsiung City, Taiwan] : Department of Public Health, Kaohsiung Medical University Original Publication: [Kaohsiung City, Taiwan, Republic of China : Kaohsiung Medical College, 1996- |
| Ιατρικοί όροι (MeSH): | Intercellular Adhesion Molecule-1*/metabolism , Intercellular Adhesion Molecule-1*/genetics , Extracellular Traps*/metabolism , Ubiquitin-Specific Peptidase 7*/metabolism , Ubiquitin-Specific Peptidase 7*/genetics , Sepsis*/metabolism , Sepsis*/chemically induced , Sepsis*/pathology , Sepsis*/genetics , Neutrophils*/metabolism , Inflammation*/metabolism , Inflammation*/pathology , Lipopolysaccharides*, Macrophages/metabolism ; Animals ; Mice ; Mice, Knockout ; Male ; Mice, Inbred C57BL ; Disease Models, Animal ; Ubiquitination |
| Περίληψη: | Sepsis is typified by organ failure due to an unchecked host reaction to infection. This study aims to explore the mechanism of ubiquitin-specific peptidase 7 (USP7) in sepsis with the involvement of intercellular adhesion molecule-1 (ICAM-1). A sepsis model was established using lipopolysaccharide (LPS) induction in WT and USP7-/- mice, and various assays were conducted to evaluate survival rates, organ damage, inflammatory markers, and protein interactions. The results revealed that USP7 expression increased in LPS-treated WT mice, and its interaction with ICAM-1 stabilized ICAM-1 through deubiquitination. USP7 knockout significantly elevated survival rates of septic mice. USP7 knockout reduced pulmonary inflammation, neutrophil extracellular trap (NET) formation, and myeloperoxidase and Cit-H3 levels in septic mice. Moreover, USP7 knockout lowered the levels of organ injury markers (creatine kinase-MB [CK-MB], troponin-I, and blood urea nitrogen [BUN]), liver enzymes (ALT and AST), and inflammatory markers (TNF-α, IL-1β, IL-6, and IL-8). Co-culture of bone marrow-derived macrophages (BMDMs) from WT mice with ICAM-1+ neutrophils elevated levels of TNF-α, IL-1β, and IL-6. These findings suggest that USP7 plays a critical role in driving sepsis-induced NET formation and inflammation by stabilizing ICAM-1. Targeting USP7 may represent a potential therapeutic approach to mitigate sepsis-related inflammation and organ damage. (© 2025 The Author(s). The Kaohsiung Journal of Medical Sciences published by John Wiley & Sons Australia, Ltd on behalf of Kaohsiung Medical University.) |
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| Grant Information: | ZT202110 Shanghai Jiao Tong University School of Medicine Technology Transfer Project; 2022hbyjxys-cyb Shanghai Ninth Hospital Research Physician Training Project |
| Contributed Indexing: | Keywords: intercellular adhesion molecule‐1; neutrophil extracellular traps; sepsis; ubiquitin‐specific peptidase 7 |
| Substance Nomenclature: | 126547-89-5 (Intercellular Adhesion Molecule-1) 0 (Lipopolysaccharides) EC 3.4.19.12 (Ubiquitin-Specific Peptidase 7) 0 (Icam1 protein, mouse) |
| Entry Date(s): | Date Created: 20251031 Date Completed: 20260509 Latest Revision: 20260813 |
| Update Code: | 20260813 |
| PubMed Central ID: | PMC13182596 |
| DOI: | 10.1002/kjm2.70133 |
| PMID: | 41170880 |
| Βάση Δεδομένων: | MEDLINE |
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