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Evaluation of harmful impact of low concentration cylindrospermopsin through zebrafish behavioral endpoints under multiple life stages.

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Τίτλος: Evaluation of harmful impact of low concentration cylindrospermopsin through zebrafish behavioral endpoints under multiple life stages.
Συγγραφείς: Fu M; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Zhang Y; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China; School of Environment, Beijing Normal University, Beijing 100875, China., Luo L; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Wu M; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Teng L; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China; School of Food Science and Health, Zhejiang A&F University, Hangzhou 311300, China., Mu J; Hebei Ocean and Fisheries Science Research, Qinhuangdao 066000, China; Hebei Marine Living Resources and Environment Key Laboratory, Qinhuangdao 066004, China., Xiao G; Hebei Ocean and Fisheries Science Research, Qinhuangdao 066000, China; Hebei Marine Living Resources and Environment Key Laboratory, Qinhuangdao 066004, China., Xu Y; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Qiu J; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: qiujing@caas.cn., Chai T; School of Food Science and Health, Zhejiang A&F University, Hangzhou 311300, China., Qian Y; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Hao G; Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs, Huzhou 313098, China; Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Huzhou 313098, China; Zhejiang Institute of Freshwater Fisheries, Huzhou 313098, China. Electronic address: Haoguijie79@126.com., Mu X; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: muxiyan@caas.cn.
Πηγή: Journal of hazardous materials [J Hazard Mater] 2025 Nov 05; Vol. 499, pp. 140228. Date of Electronic Publication: 2025 Oct 24.
Τύπος έκδοσης: Journal Article; Research Support, Non-U.S. Gov't
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9422688 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3336 (Electronic) Linking ISSN: 03043894 NLM ISO Abbreviation: J Hazard Mater Subsets: MEDLINE
Imprint Name(s): Original Publication: Amsterdam : Elsevier
Ιατρικοί όροι (MeSH): Zebrafish*/physiology , Zebrafish*/embryology , Zebrafish*/growth & development , Behavior, Animal*/drug effects , Uracil*/analogs & derivatives , Uracil*/toxicity , Water Pollutants, Chemical*/toxicity, Alkaloids/toxicity ; Larva/drug effects ; Bacterial Toxins/toxicity ; Embryo, Nonmammalian/drug effects ; Heart Rate/drug effects ; Reproduction/drug effects ; Sexual Behavior, Animal/drug effects ; Animals ; Cyanobacteria Toxins ; Male ; Female
Περίληψη: Cylindrospermopsin (CYN) is a toxic substance produced by cyanobacteria. It has attracted much attention due to its widespread global distribution, bioaccumulation and multi organ toxicity. This study aims to explore the negative effects of environmentally relevant concentrations (0.5-2000 μg/L) of CYN using different life stages of zebrafish. We found that CYN exposure decreased the spontaneous movement of embryos, reduced the swimming distance and average speed of larvae post 6-days exposure, and interfered with the courtship performance of adult fish after 14 days exposure with effective concentration of 100 and 0.5 μg/L respectively. These behavioral changes were companied by slowed embryonic heart rate, decreased body length in hatched larvae and reduced spawning, fertilization rates, abnormal level of sex hormone in adults. Further analysis indicated a high association between inhibited courtship behaviors and reproductive effects induced by CYN. Transcriptomic analysis of zebrafish larvae showed that genes related to heart development, cytoskeletal structure in muscle cells, and energy metabolism were significantly altered after CYN exposure. These transcriptional changes are consistent with the observed phenotypic symptoms. These findings offer a comprehensive understanding of the toxic effects of CYN exposure on zebrafish from a behavioral perspective.
(Copyright © 2025 Elsevier B.V. All rights reserved.)
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Behavior; Cylindrospermopsin; Developmental impact; Reproductive Effect; Zebrafish
Substance Nomenclature: 2JIZ556BA3 (cylindrospermopsin)
0 (Cyanobacteria Toxins)
0 (Alkaloids)
56HH86ZVCT (Uracil)
0 (Water Pollutants, Chemical)
0 (Bacterial Toxins)
Entry Date(s): Date Created: 20251030 Date Completed: 20251122 Latest Revision: 20260623
Update Code: 20260623
DOI: 10.1016/j.jhazmat.2025.140228
PMID: 41167163
Βάση Δεδομένων: MEDLINE
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Evaluation of harmful impact of low concentration cylindrospermopsin through zebrafish behavioral endpoints under multiple life stages.
– Name: Author
  Label: Authors
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  Data: <searchLink fieldCode="AU" term="%22Fu+M%22">Fu M</searchLink>; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China.<br /><searchLink fieldCode="AU" term="%22Zhang+Y%22">Zhang Y</searchLink>; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China; School of Environment, Beijing Normal University, Beijing 100875, China.<br /><searchLink fieldCode="AU" term="%22Luo+L%22">Luo L</searchLink>; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China.<br /><searchLink fieldCode="AU" term="%22Wu+M%22">Wu M</searchLink>; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China.<br /><searchLink fieldCode="AU" term="%22Teng+L%22">Teng L</searchLink>; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China; School of Food Science and Health, Zhejiang A&F University, Hangzhou 311300, China.<br /><searchLink fieldCode="AU" term="%22Mu+J%22">Mu J</searchLink>; Hebei Ocean and Fisheries Science Research, Qinhuangdao 066000, China; Hebei Marine Living Resources and Environment Key Laboratory, Qinhuangdao 066004, China.<br /><searchLink fieldCode="AU" term="%22Xiao+G%22">Xiao G</searchLink>; Hebei Ocean and Fisheries Science Research, Qinhuangdao 066000, China; Hebei Marine Living Resources and Environment Key Laboratory, Qinhuangdao 066004, China.<br /><searchLink fieldCode="AU" term="%22Xu+Y%22">Xu Y</searchLink>; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China.<br /><searchLink fieldCode="AU" term="%22Qiu+J%22">Qiu J</searchLink>; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: qiujing@caas.cn.<br /><searchLink fieldCode="AU" term="%22Chai+T%22">Chai T</searchLink>; School of Food Science and Health, Zhejiang A&F University, Hangzhou 311300, China.<br /><searchLink fieldCode="AU" term="%22Qian+Y%22">Qian Y</searchLink>; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China.<br /><searchLink fieldCode="AU" term="%22Hao+G%22">Hao G</searchLink>; Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs, Huzhou 313098, China; Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Huzhou 313098, China; Zhejiang Institute of Freshwater Fisheries, Huzhou 313098, China. Electronic address: Haoguijie79@126.com.<br /><searchLink fieldCode="AU" term="%22Mu+X%22">Mu X</searchLink>; State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: muxiyan@caas.cn.
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  Data: <searchLink fieldCode="JN" term="%229422688%22">Journal of hazardous materials</searchLink> [J Hazard Mater] 2025 Nov 05; Vol. 499, pp. 140228. <i>Date of Electronic Publication: </i>2025 Oct 24.
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  Data: Journal Article; Research Support, Non-U.S. Gov't
– Name: Language
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  Data: English
– Name: TitleSource
  Label: Journal Info
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier%22">Elsevier </searchLink><i>Country of Publication: </i>Netherlands <i>NLM ID: </i>9422688 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1873-3336 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2203043894%22">03043894 </searchLink><i>NLM ISO Abbreviation: </i>J Hazard Mater <i>Subsets: </i>MEDLINE
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  Label: Imprint Name(s)
  Group: PubInfo
  Data: <i>Original Publication</i>: Amsterdam : Elsevier
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Zebrafish%22">Zebrafish*</searchLink>/<searchLink fieldCode="MM" term="%22Zebrafish+physiology%22">physiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Zebrafish%22">Zebrafish*</searchLink>/<searchLink fieldCode="MM" term="%22Zebrafish+embryology%22">embryology</searchLink> <br /><searchLink fieldCode="MM" term="%22Zebrafish%22">Zebrafish*</searchLink>/<searchLink fieldCode="MM" term="%22Zebrafish+growth+%26+development%22">growth & development</searchLink> <br /><searchLink fieldCode="MM" term="%22Behavior%2C+Animal%22">Behavior, Animal*</searchLink>/<searchLink fieldCode="MM" term="%22Behavior%2C+Animal+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Uracil%22">Uracil*</searchLink>/<searchLink fieldCode="MM" term="%22Uracil+analogs+%26+derivatives%22">analogs & derivatives</searchLink> <br /><searchLink fieldCode="MM" term="%22Uracil%22">Uracil*</searchLink>/<searchLink fieldCode="MM" term="%22Uracil+toxicity%22">toxicity</searchLink> <br /><searchLink fieldCode="MM" term="%22Water+Pollutants%2C+Chemical%22">Water Pollutants, Chemical*</searchLink>/<searchLink fieldCode="MM" term="%22Water+Pollutants%2C+Chemical+toxicity%22">toxicity</searchLink><br /><searchLink fieldCode="MH" term="%22Alkaloids%22">Alkaloids</searchLink>/<searchLink fieldCode="MH" term="%22Alkaloids+toxicity%22">toxicity</searchLink> ; <searchLink fieldCode="MH" term="%22Larva%22">Larva</searchLink>/<searchLink fieldCode="MH" term="%22Larva+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22Bacterial+Toxins%22">Bacterial Toxins</searchLink>/<searchLink fieldCode="MH" term="%22Bacterial+Toxins+toxicity%22">toxicity</searchLink> ; <searchLink fieldCode="MH" term="%22Embryo%2C+Nonmammalian%22">Embryo, Nonmammalian</searchLink>/<searchLink fieldCode="MH" term="%22Embryo%2C+Nonmammalian+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22Heart+Rate%22">Heart Rate</searchLink>/<searchLink fieldCode="MH" term="%22Heart+Rate+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22Reproduction%22">Reproduction</searchLink>/<searchLink fieldCode="MH" term="%22Reproduction+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22Sexual+Behavior%2C+Animal%22">Sexual Behavior, Animal</searchLink>/<searchLink fieldCode="MH" term="%22Sexual+Behavior%2C+Animal+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22Animals%22">Animals</searchLink> ; <searchLink fieldCode="MH" term="%22Cyanobacteria+Toxins%22">Cyanobacteria Toxins</searchLink> ; <searchLink fieldCode="MH" term="%22Male%22">Male</searchLink> ; <searchLink fieldCode="MH" term="%22Female%22">Female</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Cylindrospermopsin (CYN) is a toxic substance produced by cyanobacteria. It has attracted much attention due to its widespread global distribution, bioaccumulation and multi organ toxicity. This study aims to explore the negative effects of environmentally relevant concentrations (0.5-2000 μg/L) of CYN using different life stages of zebrafish. We found that CYN exposure decreased the spontaneous movement of embryos, reduced the swimming distance and average speed of larvae post 6-days exposure, and interfered with the courtship performance of adult fish after 14 days exposure with effective concentration of 100 and 0.5 μg/L respectively. These behavioral changes were companied by slowed embryonic heart rate, decreased body length in hatched larvae and reduced spawning, fertilization rates, abnormal level of sex hormone in adults. Further analysis indicated a high association between inhibited courtship behaviors and reproductive effects induced by CYN. Transcriptomic analysis of zebrafish larvae showed that genes related to heart development, cytoskeletal structure in muscle cells, and energy metabolism were significantly altered after CYN exposure. These transcriptional changes are consistent with the observed phenotypic symptoms. These findings offer a comprehensive understanding of the toxic effects of CYN exposure on zebrafish from a behavioral perspective.<br /> (Copyright © 2025 Elsevier B.V. All rights reserved.)
– Name: Abstract
  Label: Competing Interests
  Group: Ab
  Data: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>Behavior; Cylindrospermopsin; Developmental impact; Reproductive Effect; Zebrafish
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 2JIZ556BA3 (cylindrospermopsin)<br />0 (Cyanobacteria Toxins)<br />0 (Alkaloids)<br />56HH86ZVCT (Uracil)<br />0 (Water Pollutants, Chemical)<br />0 (Bacterial Toxins)
– Name: DateEntry
  Label: Entry Date(s)
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  Data: <i>Date Created: </i>20251030 <i>Date Completed: </i>20251122 <i>Latest Revision: </i>20260623
– Name: DateUpdate
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  Data: 20260623
– Name: DOI
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  Data: 10.1016/j.jhazmat.2025.140228
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  Data: 41167163
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=41167163
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        Value: 10.1016/j.jhazmat.2025.140228
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      – Code: eng
        Text: English
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        StartPage: 140228
    Subjects:
      – SubjectFull: Alkaloids toxicity
        Type: general
      – SubjectFull: Larva drug effects
        Type: general
      – SubjectFull: Bacterial Toxins toxicity
        Type: general
      – SubjectFull: Embryo, Nonmammalian drug effects
        Type: general
      – SubjectFull: Heart Rate drug effects
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      – SubjectFull: Animals
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      – SubjectFull: Cyanobacteria Toxins
        Type: general
      – SubjectFull: Male
        Type: general
      – SubjectFull: Female
        Type: general
      – SubjectFull: Zebrafish physiology
        Type: general
      – SubjectFull: Zebrafish embryology
        Type: general
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      – SubjectFull: Behavior, Animal drug effects
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      – SubjectFull: Uracil analogs & derivatives
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      – TitleFull: Evaluation of harmful impact of low concentration cylindrospermopsin through zebrafish behavioral endpoints under multiple life stages.
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