Academic Journal
The Hidden Bacterial World of Natural Springs: Insight Through MALDI-TOF-Mass Spectrometry.
| Τίτλος: | The Hidden Bacterial World of Natural Springs: Insight Through MALDI-TOF-Mass Spectrometry. |
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| Συγγραφείς: | Abbas R; Department of Biotechnology, Quaid-i-Azam University Islamabad, Islamabad, Pakistan., Haris M; Department of Biotechnology, Quaid-i-Azam University Islamabad, Islamabad, Pakistan., Rahman S; Department of Biotechnology, Quaid-i-Azam University Islamabad, Islamabad, Pakistan., Shahid SA; Department of Biotechnology, Quaid-i-Azam University Islamabad, Islamabad, Pakistan.; Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Oman., Gul T; Microbiology and Immunology Department, University of Otago, Dunedin, New Zealand., Farooq A; Public Health Laboratories Division, National Institute of Health, Islamabad, Pakistan., Ismail A; School of Medicine, Taif University, Taif, Saudi Arabia., Alotaibi MO; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.; Environmental and Biomaterial Unit, Natural and Health Sciences Research Center, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia., El-Saber Batiha G; Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour, AlBeheira, Egypt., Ali M; Department of Biotechnology, Quaid-i-Azam University Islamabad, Islamabad, Pakistan. |
| Πηγή: | MicrobiologyOpen [Microbiologyopen] 2025 Dec; Vol. 14 (6), pp. e70021. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101588314 Publication Model: Print Cited Medium: Internet ISSN: 2045-8827 (Electronic) Linking ISSN: 20458827 NLM ISO Abbreviation: Microbiologyopen Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Oxford : Wiley-Blackwell |
| Ιατρικοί όροι (MeSH): | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*/methods , Bacteria*/classification , Bacteria*/isolation & purification , Bacteria*/chemistry , Bacteria*/genetics , Natural Springs*/microbiology, RNA, Ribosomal, 16S/genetics ; Biodiversity |
| Περίληψη: | This study explored the microbial diversity in two underexplored natural springs, Arincho Chumik and Chutron, located in the Shigar Valley, Gilgit Baltistan, utilizing a culture-centered method combined with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS). The outcomes revealed a diverse microbial landscape, with a total of 18 unique bacterial strains isolated, comprising nine from each spring. From the total 18 isolated strains, 7 (39%) were noticed to be gram-positive, while 11 (61%) were gram-negative. Interestingly, species like Brevundimonas and Acinetobacter were present in Arincho and Chutron springs, respectively, highlighting the unique physicochemical environments and their impact on microbial populations. The examination also uncovers the existence of pigment-producing bacteria, suggesting potential biotechnological applications. The chilly freshwater spring of Arincho possessed certain opportunistic bacteria, including Bacillus cereus, Dietzia cinnamea, and Microbacterium species. Likewise, human-related microorganisms like Micrococcus leuteus were also identified in samples from the Chutron thermal spring. Additionally, the recognition of opportunistic pathogens among the strains underlines the health effects for the local communities, especially for the elderly and immune-deficient individuals. The quality of these water resources ought to be supervised by regulatory authorities to decrease public health risks and pathogen transmission. (© 2025 The Author(s). MicrobiologyOpen published by John Wiley & Sons Ltd.) |
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| Grant Information: | The research was funded by Princess Nourah bint Abdulrahman University, Saudi Arabia, Project No. (PNURSP2025R101). |
| Contributed Indexing: | Keywords: Gilgit Baltistan; MALDI‐TOF‐MS; Shigar; freshwater microbial diversity; natural springs; opportunistic pathogens |
| Substance Nomenclature: | 0 (RNA, Ribosomal, 16S) |
| Entry Date(s): | Date Created: 20251030 Date Completed: 20251030 Latest Revision: 20260727 |
| Update Code: | 20260728 |
| PubMed Central ID: | PMC12571964 |
| DOI: | 10.1002/mbo3.70021 |
| PMID: | 41163365 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2045-8827 |
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| DOI: | 10.1002/mbo3.70021 |