Academic Journal
Nanoliter-Fabricated Paper-Based Colorimetric Lateral Flow Strip for Urea Detection.
| Τίτλος: | Nanoliter-Fabricated Paper-Based Colorimetric Lateral Flow Strip for Urea Detection. |
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| Συγγραφείς: | Kongkaew S; Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.; Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand., Cotchim S; Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.; Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand., Limbut W; Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.; Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.; Forensic Science Innovation and Service Center, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand. |
| Πηγή: | Biosensors [Biosensors (Basel)] 2025 Oct 11; Vol. 15 (10). Date of Electronic Publication: 2025 Oct 11. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: MDPI Pub Country of Publication: Switzerland NLM ID: 101609191 Publication Model: Electronic Cited Medium: Internet ISSN: 2079-6374 (Electronic) Linking ISSN: 20796374 NLM ISO Abbreviation: Biosensors (Basel) Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI Pub. |
| Ιατρικοί όροι (MeSH): | Urea*/analysis , Colorimetry* , Biosensing Techniques*, Urease/chemistry ; Paper ; Humans ; Enzymes, Immobilized |
| Περίληψη: | A nanoliter-scale fabrication method was applied to construct a colorimetric lateral flow strip for urea detection (Urea-CLFS). The device involves two main papers: a nitrocellulose membrane (NC-Mb) for urease enzyme immobilization and chromatography paper (CH-PP) containing a phenol red indicator. Urea-CLFS is a tool for detecting urea that is based on enzyme catalysis and the change in color of phenol red when urea is present. The Urea-CLFS fabrication was made possible by the minimal amount of nanoliters used in reagent consumption. The use of small arrays of phenol red dots provides a higher response result compared to single dots applied on CH-PP. To find the most effective design, it analyzed how urease was aligned on NC-Mb horizontally and vertically. According to our findings, the vertical alignment of the urease enzyme on NC-Mb leads to a prolonged reaction time, which leads to higher product production. The optimization process included optimizing various parameters, including the layer number of phenol red on CH-PP, phenol red concentration, urease concentration, reaction time, and sample volume. Under optimal conditions, the Urea-CLFS provided a linear range of 0.25-8.0 mmol L-1 with an LOD of 0.34 mmol L-1, which is sufficient for human health diagnostics. The accuracy of the Urea-CLFS was demonstrated by the recovery of the human urine sample between 95 ± 3% and 103 ± 3% (n = 3). |
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| Grant Information: | a Postdoctoral Fellowship from Prince of Songkla University |
| Contributed Indexing: | Keywords: biosensor; colorimetric; lateral flow strip; miniaturization; urea |
| Substance Nomenclature: | 8W8T17847W (Urea) EC 3.5.1.5 (Urease) 0 (Enzymes, Immobilized) |
| Entry Date(s): | Date Created: 20251028 Date Completed: 20251028 Latest Revision: 20251101 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12562505 |
| DOI: | 10.3390/bios15100688 |
| PMID: | 41149341 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2079-6374 |
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| DOI: | 10.3390/bios15100688 |