Academic Journal
Tepsin and AP4 mediate transport from the trans-Golgi to the plant-like vacuole in toxoplasma.
| Τίτλος: | Tepsin and AP4 mediate transport from the trans-Golgi to the plant-like vacuole in toxoplasma. |
|---|---|
| Συγγραφείς: | Grech J; Experimental Parasitology, Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität, LMU, Munich, Germany., Shinde AP; Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic.; Division of Infectious Diseases, Department of Medicine and Department of Biological Sciences, University of Alberta, Edmonton, Canada., Periz J; Experimental Parasitology, Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität, LMU, Munich, Germany., Singer M; Experimental Parasitology, Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität, LMU, Munich, Germany.; Integrative Parasitology, Center for Infectious Diseases, Heidelberg University Medical Faculty, Heidelberg, Germany., Gras S; Experimental Parasitology, Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität, LMU, Munich, Germany., Forné I; Zentrallabor für Proteinanalytik, Biomedical Center Munich, Ludwig-Maximilians-Universität, LMU, Munich, Germany., Klingl A; Pflanzliche Entwicklungsbiologie, Biozentrum der Ludwig-Maximilians-Universität, Munich, Germany., Dacks JB; Division of Infectious Diseases, Department of Medicine and Department of Biological Sciences, University of Alberta, Edmonton, Canada.; Centre for LIfe's Origin and Evolution, Department of Genetics, Evolution, & Environment, University College, London, UK.; Institute of Parasitology, Biology Centre, Czech Academy of Sciences , České Budějovice (Budweis), Czech Republic., Jiménez-Ruiz E; Experimental Parasitology, Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität, LMU, Munich, Germany., Meissner M; Experimental Parasitology, Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität, LMU, Munich, Germany. |
| Πηγή: | The Journal of cell biology [J Cell Biol] 2025 Dec 01; Vol. 224 (12). Date of Electronic Publication: 2025 Oct 13. |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 0375356 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1540-8140 (Electronic) Linking ISSN: 00219525 NLM ISO Abbreviation: J Cell Biol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: New York : Rockefeller University Press |
| Ιατρικοί όροι (MeSH): | Toxoplasma*/metabolism , Toxoplasma*/genetics , Vacuoles*/metabolism , Protozoan Proteins*/metabolism , Protozoan Proteins*/genetics , Adaptor Protein Complex 4*/metabolism , Adaptor Protein Complex 4*/genetics , trans-Golgi Network*/metabolism, Golgi Apparatus/metabolism ; Clathrin/metabolism ; Protein Transport |
| Περίληψη: | Apicomplexan parasites are obligate intracellular pathogens possessing unique organelles but lacking several components of the membrane trafficking machinery conserved in other eukaryotes. While some of these components have been lost during evolution, others remain undetectable by standard bioinformatics approaches. Using a conditional splitCas9 system in Toxoplasma gondii, we previously identified TGGT1_301410, a hypothetical gene conserved among apicomplexans, as a potential trafficking factor. Here, we show that TGGT1_301410 is a distant ortholog of T. gondii tepsin (TgTEP), localized to the trans-Golgi and functioning as an accessory protein of the adaptor protein complex 4 (AP4). We demonstrate that AP4-TgTEP is essential for the actin-dependent transport of vesicles to the plant-like vacuole (PLVAC) and Golgi organization. Notably, our findings reveal that, unlike in metazoans, the AP4 complex in T. gondii utilizes clathrin as a coat protein, a mechanism more closely aligned with that of plants. These results underscore a conserved yet functionally adapted vesicular transport system in Apicomplexa. (© 2025 Grech et al.) |
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| Grant Information: | SPP2225 Deutsche Forschungsgemeinschaft; ME 2675/7-1 Deutsche Forschungsgemeinschaft; JI 463/2-2 Deutsche Forschungsgemeinschaft; INST 86/1831-1 Deutsche Forschungsgemeinschaft |
| Substance Nomenclature: | 0 (Protozoan Proteins) 0 (Adaptor Protein Complex 4) 0 (Clathrin) |
| Entry Date(s): | Date Created: 20251013 Date Completed: 20251013 Latest Revision: 20260624 |
| Update Code: | 20260625 |
| PubMed Central ID: | PMC12517565 |
| DOI: | 10.1083/jcb.202312109 |
| PMID: | 41082686 |
| Βάση Δεδομένων: | MEDLINE |
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