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Achieving partial nitritation-anammox in membrane-aerated biofilm reactor by hydroxylamine addition.

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Τίτλος: Achieving partial nitritation-anammox in membrane-aerated biofilm reactor by hydroxylamine addition.
Συγγραφείς: Mei N; Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing, 100044, China; Beijing ENFI Environmental Protection Co., Ltd., Beijing, 100038, China., Jia F; Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing, 100044, China., Liu Y; Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing, 100044, China., Li Y; Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing, 100044, China., Qi X; Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing, 100044, China., Han B; Department of Municipal and Environmental Engineering, Hebei University of Architecture, Zhangjiakou, 075000, China., Zhang X; Sichuan Animal Sciences Academy, Chengdu, 610066, China., Liu T; Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, 999077, Hong Kong, China., Yao H; Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing, 100044, China. Electronic address: yaohongts@163.com.
Πηγή: Journal of environmental management [J Environ Manage] 2025 Nov; Vol. 394, pp. 127404. Date of Electronic Publication: 2025 Oct 01.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Academic Press Country of Publication: England NLM ID: 0401664 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-8630 (Electronic) Linking ISSN: 03014797 NLM ISO Abbreviation: J Environ Manage Subsets: MEDLINE
Imprint Name(s): Original Publication: London ; New York, Academic Press.
Ιατρικοί όροι (MeSH): Bioreactors*/microbiology , Hydroxylamine*/chemistry , Hydroxylamine*/metabolism , Water Purification*/instrumentation , Water Purification*/methods , Anaerobic Ammonia Oxidation* , Biofilms*, Nitrites/metabolism ; Nitrogen/metabolism ; Ammonia/metabolism ; Bacteria/metabolism ; Waste Disposal, Fluid/instrumentation ; Waste Disposal, Fluid/methods ; Oxidation-Reduction
Περίληψη: The membrane-aerated biofilm reactor (MABR) is naturally suitable for partial nitritation-anammox (PN/A) because aerobic and anaerobic microorganisms can grow in different biofilm layers. However, suppressing nitrite-oxidizing bacteria (NOB) in the oxygen-rich inner MABR biofilms remains challenging, while anammox bacteria (AnAOB) in the outer layer are more vulnerable to many harsh treatments. This study demonstrated the strategy of applying hydroxylamine (NH2OH) to achieve stable PN/A in MABR. Over 250 days, long-term experiments showed that low dissolved oxygen (0.06-0.20 mg L-1) could not suppress NOB, while both continuous and intermittent 10 mg L-1 NH2OH addition not only achieved effective NOB suppression but also significantly promoted ammonia-oxidizing bacteria and AnAOB. Consequently, the nitrogen removal efficiency increased from 74.0 ± 1.1 % to 91.7 ± 1.6 %. Fundamentally, NH2OH addition rapidly changed the transcription levels of key functional genes in membrane biofilms, resulting in a significant decrease in nxrB transcription level by 68.6 ± 3.8 % and an increase in those of amoA and hzsB by 1835.8 ± 307.2 % and 314.2 ± 112.7 %, respectively. NH2OH addition resulted in the temporary accumulation of hydrazine and nitric oxide at low levels, which collectively contributed to NOB suppression. Particularly, Nitrospira, "Ca. Nitrotoga" and Nitrolancea (all three distinct NOB genera present in MABR biofilm) relative abundances decreased by 48.6 ± 7.5 %, 10.8 ± 2.5 %, and 75.0 ± 6.8 % respectively, which alleviated NOB adaptation risk. Therefore, NH2OH can be used to support NOB suppression in MABR.
(Copyright © 2025. Published by Elsevier Ltd.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Biofilm; Hydroxylamine; Membrane-aerated biofilm reactor; Nitrite-oxidizing bacteria suppression; Partial nitritation-anammox
Substance Nomenclature: 2FP81O2L9Z (Hydroxylamine)
0 (Nitrites)
N762921K75 (Nitrogen)
7664-41-7 (Ammonia)
Entry Date(s): Date Created: 20251002 Date Completed: 20260225 Latest Revision: 20260225
Update Code: 20260226
DOI: 10.1016/j.jenvman.2025.127404
PMID: 41038100
Βάση Δεδομένων: MEDLINE
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Achieving partial nitritation-anammox in membrane-aerated biofilm reactor by hydroxylamine addition.
– Name: Author
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  Data: <searchLink fieldCode="AU" term="%22Mei+N%22">Mei N</searchLink>; Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing, 100044, China; Beijing ENFI Environmental Protection Co., Ltd., Beijing, 100038, China.<br /><searchLink fieldCode="AU" term="%22Jia+F%22">Jia F</searchLink>; Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing, 100044, China.<br /><searchLink fieldCode="AU" term="%22Liu+Y%22">Liu Y</searchLink>; Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing, 100044, China.<br /><searchLink fieldCode="AU" term="%22Li+Y%22">Li Y</searchLink>; Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing, 100044, China.<br /><searchLink fieldCode="AU" term="%22Qi+X%22">Qi X</searchLink>; Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing, 100044, China.<br /><searchLink fieldCode="AU" term="%22Han+B%22">Han B</searchLink>; Department of Municipal and Environmental Engineering, Hebei University of Architecture, Zhangjiakou, 075000, China.<br /><searchLink fieldCode="AU" term="%22Zhang+X%22">Zhang X</searchLink>; Sichuan Animal Sciences Academy, Chengdu, 610066, China.<br /><searchLink fieldCode="AU" term="%22Liu+T%22">Liu T</searchLink>; Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, 999077, Hong Kong, China.<br /><searchLink fieldCode="AU" term="%22Yao+H%22">Yao H</searchLink>; Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing, 100044, China. Electronic address: yaohongts@163.com.
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  Data: <searchLink fieldCode="JN" term="%220401664%22">Journal of environmental management</searchLink> [J Environ Manage] 2025 Nov; Vol. 394, pp. 127404. <i>Date of Electronic Publication: </i>2025 Oct 01.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Academic+Press%22">Academic Press </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>0401664 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1095-8630 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2203014797%22">03014797 </searchLink><i>NLM ISO Abbreviation: </i>J Environ Manage <i>Subsets: </i>MEDLINE
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  Data: <i>Original Publication</i>: London ; New York, Academic Press.
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  Data: <searchLink fieldCode="MM" term="%22Bioreactors%22">Bioreactors*</searchLink>/<searchLink fieldCode="MM" term="%22Bioreactors+microbiology%22">microbiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Hydroxylamine%22">Hydroxylamine*</searchLink>/<searchLink fieldCode="MM" term="%22Hydroxylamine+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Hydroxylamine%22">Hydroxylamine*</searchLink>/<searchLink fieldCode="MM" term="%22Hydroxylamine+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Water+Purification%22">Water Purification*</searchLink>/<searchLink fieldCode="MM" term="%22Water+Purification+instrumentation%22">instrumentation</searchLink> <br /><searchLink fieldCode="MM" term="%22Water+Purification%22">Water Purification*</searchLink>/<searchLink fieldCode="MM" term="%22Water+Purification+methods%22">methods</searchLink> <br /><searchLink fieldCode="MM" term="%22Anaerobic+Ammonia+Oxidation%22">Anaerobic Ammonia Oxidation*</searchLink> <br /><searchLink fieldCode="MM" term="%22Biofilms%22">Biofilms*</searchLink><br /><searchLink fieldCode="MH" term="%22Nitrites%22">Nitrites</searchLink>/<searchLink fieldCode="MH" term="%22Nitrites+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Nitrogen%22">Nitrogen</searchLink>/<searchLink fieldCode="MH" term="%22Nitrogen+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Ammonia%22">Ammonia</searchLink>/<searchLink fieldCode="MH" term="%22Ammonia+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Bacteria%22">Bacteria</searchLink>/<searchLink fieldCode="MH" term="%22Bacteria+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Waste+Disposal%2C+Fluid%22">Waste Disposal, Fluid</searchLink>/<searchLink fieldCode="MH" term="%22Waste+Disposal%2C+Fluid+instrumentation%22">instrumentation</searchLink> ; <searchLink fieldCode="MH" term="%22Waste+Disposal%2C+Fluid%22">Waste Disposal, Fluid</searchLink>/<searchLink fieldCode="MH" term="%22Waste+Disposal%2C+Fluid+methods%22">methods</searchLink> ; <searchLink fieldCode="MH" term="%22Oxidation-Reduction%22">Oxidation-Reduction</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The membrane-aerated biofilm reactor (MABR) is naturally suitable for partial nitritation-anammox (PN/A) because aerobic and anaerobic microorganisms can grow in different biofilm layers. However, suppressing nitrite-oxidizing bacteria (NOB) in the oxygen-rich inner MABR biofilms remains challenging, while anammox bacteria (AnAOB) in the outer layer are more vulnerable to many harsh treatments. This study demonstrated the strategy of applying hydroxylamine (NH<subscript>2</subscript>OH) to achieve stable PN/A in MABR. Over 250 days, long-term experiments showed that low dissolved oxygen (0.06-0.20 mg L<superscript>-1</superscript>) could not suppress NOB, while both continuous and intermittent 10 mg L<superscript>-1</superscript> NH<subscript>2</subscript>OH addition not only achieved effective NOB suppression but also significantly promoted ammonia-oxidizing bacteria and AnAOB. Consequently, the nitrogen removal efficiency increased from 74.0 ± 1.1 % to 91.7 ± 1.6 %. Fundamentally, NH<subscript>2</subscript>OH addition rapidly changed the transcription levels of key functional genes in membrane biofilms, resulting in a significant decrease in nxrB transcription level by 68.6 ± 3.8 % and an increase in those of amoA and hzsB by 1835.8 ± 307.2 % and 314.2 ± 112.7 %, respectively. NH<subscript>2</subscript>OH addition resulted in the temporary accumulation of hydrazine and nitric oxide at low levels, which collectively contributed to NOB suppression. Particularly, Nitrospira, "Ca. Nitrotoga" and Nitrolancea (all three distinct NOB genera present in MABR biofilm) relative abundances decreased by 48.6 ± 7.5 %, 10.8 ± 2.5 %, and 75.0 ± 6.8 % respectively, which alleviated NOB adaptation risk. Therefore, NH<subscript>2</subscript>OH can be used to support NOB suppression in MABR.<br /> (Copyright © 2025. Published by Elsevier Ltd.)
– Name: Abstract
  Label: Competing Interests
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  Data: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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  Data: <i>Keywords: </i>Biofilm; Hydroxylamine; Membrane-aerated biofilm reactor; Nitrite-oxidizing bacteria suppression; Partial nitritation-anammox
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  Data: 2FP81O2L9Z (Hydroxylamine)<br />0 (Nitrites)<br />N762921K75 (Nitrogen)<br />7664-41-7 (Ammonia)
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        StartPage: 127404
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      – TitleFull: Achieving partial nitritation-anammox in membrane-aerated biofilm reactor by hydroxylamine addition.
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