Academic Journal
Chitosan-based Oral DNA vaccine elicits mucosal immunity and reduces Helicobacter pylori colonization in mice.
| Τίτλος: | Chitosan-based Oral DNA vaccine elicits mucosal immunity and reduces Helicobacter pylori colonization in mice. |
|---|---|
| Συγγραφείς: | Chan YL; Department of Chemistry, Chinese Culture University, Taipei, Taiwan. Electronic address: zyl13@ulive.pccu.edu.tw., Chao YP; Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan., Li TL; Genomics Research Center, Academia Sinica, Taipei, Taiwan., Liao KW; Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, Taiwan. |
| Πηγή: | Vaccine [Vaccine] 2025 Oct 03; Vol. 64, pp. 127692. Date of Electronic Publication: 2025 Sep 09. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8406899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2518 (Electronic) Linking ISSN: 0264410X NLM ISO Abbreviation: Vaccine Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam, The Netherlands : Elsevier Science Original Publication: [Guildford, Surrey, UK] : Butterworths, [c1983- |
| Ιατρικοί όροι (MeSH): | Chitosan*/administration & dosage , Vaccines, DNA*/immunology , Vaccines, DNA*/administration & dosage , Helicobacter pylori*/immunology , Helicobacter pylori*/genetics , Helicobacter Infections*/prevention & control , Helicobacter Infections*/immunology , Bacterial Vaccines*/immunology , Bacterial Vaccines*/administration & dosage , Immunity, Mucosal*, Urease/immunology ; Urease/genetics ; Nanoparticles/administration & dosage ; Interferon-gamma/metabolism ; Antibodies, Bacterial/blood ; Interleukin-17/metabolism ; Interleukin-17/immunology ; Animals ; Administration, Oral ; Mice ; Female ; Immunoglobulin G ; Immunoglobulin A ; Mice, Inbred BALB C |
| Περίληψη: | Oral vaccination offers a promising strategy for controlling Helicobacter pylori infection, particularly in the face of rising antibiotic resistance and reinfection rates. In this study, we developed a chitosan nanoparticle-mediated oral DNA vaccine encoding the urease B subunit of H. pylori. The chitosan-DNA nanoparticles exhibited stability under simulated gastric and duodenal conditions and demonstrated efficient transgene expression in vivo without cytotoxicity. Oral administration of the vaccine induced strong mucosal IgA and systemic IgG responses, accompanied by elevated IFN-γ and IL-17 production. Immunized mice exhibited a 150-fold reduction in gastric H. pylori colonization and significant inhibition of urease activity compared to controls. Histological analysis revealed enhanced immune cell infiltration associated with bacterial clearance. These findings suggest that chitosan-based oral DNA vaccination can elicit protective mucosal and systemic immunity and represents a promising approach for the prevention of H. pylori infection. (Copyright © 2025. Published by Elsevier Ltd.) |
| Competing Interests: | Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Yi-Lin Chan reports financial support was provided by National Science and Technology Council, Taiwan. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Chitosan nanoparticles; Helicobacter pylori; Mucosal immunity; Oral DNA vaccine; Urease B |
| Substance Nomenclature: | 9012-76-4 (Chitosan) 0 (Vaccines, DNA) EC 3.5.1.5 (Urease) 0 (Bacterial Vaccines) 0 (Immunoglobulin G) 82115-62-6 (Interferon-gamma) 0 (Antibodies, Bacterial) 0 (Immunoglobulin A) 0 (Interleukin-17) |
| Entry Date(s): | Date Created: 20250910 Date Completed: 20251006 Latest Revision: 20251006 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.vaccine.2025.127692 |
| PMID: | 40930041 |
| Βάση Δεδομένων: | MEDLINE |
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