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Urease-Mediated Reversible Calcium Carbonate Mineralization for Single Living Yeast Cell Nanoencapsulation.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Urease-Mediated Reversible Calcium Carbonate Mineralization for Single Living Yeast Cell Nanoencapsulation.
Συγγραφείς: Wang R; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China., Zhang Y; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China., Wang K; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China., Yang W; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China., Wang G; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China., Zhao C; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.
Πηγή: Small (Weinheim an der Bergstrasse, Germany) [Small] 2025 Oct; Vol. 21 (43), pp. e06031. Date of Electronic Publication: 2025 Sep 10.
Τύπος έκδοσης: Journal Article; Research Support, Non-U.S. Gov't
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101235338 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1613-6829 (Electronic) Linking ISSN: 16136810 NLM ISO Abbreviation: Small Subsets: MEDLINE
Imprint Name(s): Original Publication: Weinheim, Germany : Wiley-VCH, c2005-
Ιατρικοί όροι (MeSH): Urease*/metabolism , Urease*/chemistry , Calcium Carbonate*/chemistry , Calcium Carbonate*/metabolism , Saccharomyces cerevisiae*/cytology , Saccharomyces cerevisiae*/metabolism, Enzymes, Immobilized/metabolism
Περίληψη: Modifying cells to achieve desired functions has attracted extensive attention in bioengineering and bio-manufacturing. Approaches based on cell-surface engineering have the potential to endow cells with multiple functions and also create a protective shell around them. However, such shells are generally irreversible and lack functionality, leading to various drawbacks associated with irreversible dynamics. This study describes a facile strategy for enzyme-catalyzed reversible CaCO3 mineralization for single-living yeast cell nanoencapsulation. Urease is employed as an effective catalyst and covalently immobilized onto the yeast cell surface using poly(sodium acrylate) as the mediator. CaCO3 mineralization proceeds via urease-catalyzed urea hydrolysis and coprecipitation with CaCl2. Because urease covalently bonds to the yeast cell surface, reversible mineralization is achieved through EDTA-induced decomposition and urease-induced remineralization. In addition, the encapsulated urease endowed the engineered cell with catalytic sites for urea utilization, demonstrating the multifunctionality of the shell. This strategy realizes enzyme-catalyzed reversible mineralization of single living cells and demonstrates its potential in the construction of multifunctional shells, providing a nanoscale tool for structural modification to obtain single-cell factories for application in bioengineering and bio-manufacturing.
(© 2025 Wiley‐VCH GmbH.)
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Grant Information: 52273116 National Natural Science Foundation of China; 52373171 National Natural Science Foundation of China; 52588201 National Natural Science Foundation of China; 2232058 Natural Science Foundation of Beijing Municipality
Contributed Indexing: Keywords: biomimetic mineralization; cell coating; reversible shells; ureases
Substance Nomenclature: EC 3.5.1.5 (Urease)
H0G9379FGK (Calcium Carbonate)
0 (Enzymes, Immobilized)
Entry Date(s): Date Created: 20250910 Date Completed: 20251029 Latest Revision: 20260720
Update Code: 20260720
DOI: 10.1002/smll.202506031
PMID: 40928196
Βάση Δεδομένων: MEDLINE
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