Academic Journal
High-Resolution aCGH Analysis of the Jurkat Cell Line: Copy Number Alterations and Their Association With Cancer Hallmarks.
| Τίτλος: | High-Resolution aCGH Analysis of the Jurkat Cell Line: Copy Number Alterations and Their Association With Cancer Hallmarks. |
|---|---|
| Συγγραφείς: | DA Costa LM; Department of Biological Sciences, Oncology Research Center, Federal University of Pará, Belém, Brazil., Ferreira WAS; Laboratory of Cytogenomics and Environmental Mutagenesis, Environment Section (SAMAM), Evandro Chagas Institute (IEC), Ananindeua, Brazil., Machado CB; Department of Medicine, Clinical Genetics Laboratory, Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Brazil., Amorim CKN; Laboratory of Cytogenomics and Environmental Mutagenesis, Environment Section (SAMAM), Evandro Chagas Institute (IEC), Ananindeua, Brazil., VAN DEN Berg AV; Department of Medicine, Institute of Medical Sciences, Federal University of Pará, Belém, Brazil., DE Moraes Filho MO; Department of Medicine, Clinical Genetics Laboratory, Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Brazil., DE Moraes MEA; Department of Medicine, Clinical Genetics Laboratory, Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Brazil., DE Souza LEB; Regional Blood Center of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Khayat AS; Department of Biological Sciences, Oncology Research Center, Federal University of Pará, Belém, Brazil., DE Oliveira EHC; Laboratory of Cytogenomics and Environmental Mutagenesis, Environment Section (SAMAM), Evandro Chagas Institute (IEC), Ananindeua, Brazil., Moreira-Nunes CA; Department of Biological Sciences, Oncology Research Center, Federal University of Pará, Belém, Brazil; carolfam@gmail.com.; Department of Medicine, Clinical Genetics Laboratory, Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Brazil.; Clementino Fraga Group, Central Unity, Molecular Biology Laboratory, Fortaleza, Brazil. |
| Πηγή: | Cancer genomics & proteomics [Cancer Genomics Proteomics] 2025 Sep-Oct; Vol. 22 (5), pp. 824-835. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: International Institute of Anticancer Research Country of Publication: Greece NLM ID: 101188791 Publication Model: Print Cited Medium: Internet ISSN: 1790-6245 (Electronic) Linking ISSN: 11096535 NLM ISO Abbreviation: Cancer Genomics Proteomics Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Kapandriti, Attiki, Greece : International Institute of Anticancer Research, [2004]- |
| Ιατρικοί όροι (MeSH): | Comparative Genomic Hybridization*/methods , Precursor T-Cell Lymphoblastic Leukemia-Lymphoma*/genetics , Precursor T-Cell Lymphoblastic Leukemia-Lymphoma*/pathology , DNA Copy Number Variations*, Humans ; Jurkat Cells |
| Περίληψη: | Background/aim: Cell lines serve as valuable in vitro models to study altered cellular signaling pathways, to identify mutations in key oncogenic genes, and to test potential antitumor drugs. The Jurkat cell line, for example, has provided important information about various signaling pathways in lymphoblastic leukemia, establishing most of what is currently known about T-cell receptor (TCR) signaling. However, many aspects of the genome modification of this cell line have not yet been analyzed. To identify genes of potential biological and clinical relevance in acute T-lymphoblastic leukemia (T-ALL), we performed an array comparative genomic hybridization (aCGH) approach on the widely used Jurkat cell line and examined the association of the detected copy number alterations (CNAs) with cancer hallmarks and T-ALL pathogenesis. Materials and Methods: Cells were harvested by using trypsin/EDTA from culture flasks to extract genomic DNA. aCGH experiments were performed on an Agilent microarray platform using the SurePrint G3 Cancer CGH + SNP Microarray 4×180 K. Functional enrichment analysis of all CNAs was performed with the R package g:Profiler2. The association of these alterations with key cancer hallmarks was analyzed using the Cancer Hallmarks web-tool. Results: Our analysis revealed several novel CNAs, including losses at 5p15.2, 6q27, 10q22.2, 14q11.2, 18q11.2-q12.1, and Xp22.33, as well as gains at 2p11.2, 7p21.2, 7q21.2 and 18p11.32. Genes within these regions were associated with important oncogenic pathways, including 'sustained proliferative signaling', 'tumor suppressor evasion', and 'angiogenesis promotion'. Conclusion: These findings suggest that Jurkat cells may serve as a valuable model for identifying new targets for cancer research. Further studies are required to confirm the phenotypic implications of these variants, which may open new avenues for exploring the functional impact of these alterations and their potential role in the development of therapies. (Copyright © 2025, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.) |
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| Contributed Indexing: | Keywords: Acute lymphoblastic leukemia; array-based comparative genomic hybridization; tumor cell line |
| Entry Date(s): | Date Created: 20250829 Date Completed: 20250829 Latest Revision: 20250904 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12402711 |
| DOI: | 10.21873/cgp.20539 |
| PMID: | 40883026 |
| Βάση Δεδομένων: | MEDLINE |
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