Academic Journal
Ca2+-driven cytoplasmic backflow ensures spindle anchoring in fertilized mouse eggs.
| Τίτλος: | Ca2+-driven cytoplasmic backflow ensures spindle anchoring in fertilized mouse eggs. |
|---|---|
| Συγγραφείς: | Totsuka T; Cell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA; Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan; Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: takaya.totsuka@nih.gov., Ohsugi M; Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan; Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan., Akera T; Cell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: takashi.akera@nih.gov. |
| Πηγή: | Current biology : CB [Curr Biol] 2025 Aug 18; Vol. 35 (16), pp. 3839-3850.e5. Date of Electronic Publication: 2025 Jul 25. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Cell Press Country of Publication: England NLM ID: 9107782 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0445 (Electronic) Linking ISSN: 09609822 NLM ISO Abbreviation: Curr Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Cambridge, MA : Cell Press Original Publication: London, UK : Current Biology Ltd., c1991- |
| Ιατρικοί όροι (MeSH): | Spindle Apparatus*/physiology , Spindle Apparatus*/metabolism , Calcium*/metabolism , Zygote*/physiology , Fertilization*/physiology , Cytoplasmic Streaming*/physiology , Calcium Signaling*, Meiosis/physiology ; Cytoplasm/metabolism ; Animals ; Mice ; Female ; Chromosome Segregation ; Male |
| Περίληψη: | Female meiosis is highly asymmetric, producing a large egg and a small polar body to preserve maternal storage essential for embryogenesis. To achieve asymmetric division, the egg spindle must maintain its cortical position until fertilization completes meiosis. In mice, fertilization triggers chromosome segregation, followed by spindle rotation to achieve the perpendicular orientation relative to the cortex, leading to the extrusion of one set of chromosomes. However, it was unknown how the spindle maintains its cortical position while rotating. Here, we developed a high-resolution live-imaging method to investigate spindle dynamics during fertilization. Our results indicate that Ca2+ oscillations put the brakes on spindle rotation by transiently reversing cytoplasmic streaming and that this cytoplasmic backflow secures the spindle localization at the cortex. Mechanistically, Ca2+ oscillations drive cortical actomyosin contraction to induce the cytoplasmic backflow. Altogether, this work revealed a previously unknown role of Ca2+ oscillations in maintaining spindle position, ensuring the highly asymmetric divisions inherent to female meiosis. (Published by Elsevier Inc.) |
| Competing Interests: | Declaration of interests The authors declare no competing interests. |
| References: | Nat Cell Biol. 2011 Aug 28;13(10):1252-8. (PMID: 21874009) J Reprod Dev. 2007 Dec;53(6):1207-15. (PMID: 17938555) PLoS One. 2011 Apr 08;6(4):e18392. (PMID: 21494665) Biochem Biophys Res Commun. 2016 Aug 12;477(1):14-19. (PMID: 27237977) Mol Biol Cell. 1998 Sep;9(9):2509-25. (PMID: 9725909) J Cell Biol. 2021 Oct 4;220(10):. (PMID: 34424312) Dev Biol. 2004 Aug 1;272(1):15-25. (PMID: 15242787) J Cell Biol. 2009 Dec 14;187(6):781-90. (PMID: 19995936) Dev Biol. 2000 Jan 1;217(1):179-91. (PMID: 10625545) Development. 2002 Aug;129(15):3533-44. (PMID: 12117804) Semin Cell Dev Biol. 2006 Apr;17(2):264-73. (PMID: 16730199) Biol Reprod. 2003 Mar;68(3):943-6. (PMID: 12604646) Hum Reprod. 2014 Feb;29(2):315-23. (PMID: 24166595) Nature. 1993 Nov 18;366(6452):270-3. (PMID: 8232587) Reproduction. 2006 Jan;131(1):27-34. (PMID: 16388006) Curr Top Dev Biol. 1995;30:21-62. (PMID: 7555047) Mol Biol Cell. 2006 Sep;17(9):4093-104. (PMID: 16837551) J Physiol. 1983 Jul;340:633-47. (PMID: 6411906) Biol Reprod. 1993 Jun;48(6):1251-7. (PMID: 8318579) Cell Cycle. 2013 Jun 1;12(11):1672-8. (PMID: 23656777) Nature. 2005 Oct 13;437(7061):1048-52. (PMID: 16127448) J Cell Sci. 2016 Oct 1;129(19):3609-3619. (PMID: 27550518) Open Biol. 2020 Jul;10(7):200118. (PMID: 32673518) Physiol Rev. 2003 Oct;83(4):1325-58. (PMID: 14506307) Sci Adv. 2020 Apr 03;6(14):eaaz5004. (PMID: 32284983) Nature. 1984 Apr 5-11;308(5959):548-50. (PMID: 6709062) J Cell Sci. 2023 Mar 1;136(5):. (PMID: 36250267) Science. 1992 Jul 10;257(5067):251-5. (PMID: 1321497) Cell Div. 2011 Aug 25;6:17. (PMID: 21867530) Genes Dev. 2005 Feb 15;19(4):502-13. (PMID: 15713843) Biol Reprod. 2004 Mar;70(3):535-47. (PMID: 14613897) Nat Commun. 2019 Oct 11;10(1):4651. (PMID: 31604948) Dev Biol. 2013 May 1;377(1):202-12. (PMID: 23384564) Dev Cell. 2018 Nov 5;47(3):363-376.e5. (PMID: 30293837) Dev Biol. 2000 Feb 15;218(2):299-313. (PMID: 10656771) Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772) J Embryol Exp Morphol. 1986 Mar;92:11-32. (PMID: 3723057) Genesis. 2005 Oct;43(2):71-9. (PMID: 16100711) Int Rev Cytol. 1994;152:183-222. (PMID: 8206704) J Cell Biol. 1992 Jun;117(5):921-34. (PMID: 1315785) Mol Reprod Dev. 2001 Oct;60(2):260-9. (PMID: 11553927) Nat Rev Mol Cell Biol. 2000 Oct;1(1):11-21. (PMID: 11413485) Dev Biol. 2005 Jun 1;282(1):39-54. (PMID: 15936328) J Reprod Dev. 2018 Aug 20;64(4):319-326. (PMID: 29731504) J Cell Sci. 2006 Apr 1;119(Pt 7):1213-8. (PMID: 16554437) Sci Rep. 2020 Apr 16;10(1):6556. (PMID: 32300113) Nat Commun. 2011 Aug 09;2:417. (PMID: 21829179) Cell Calcium. 2011 Jul;50(1):1-8. (PMID: 21529938) Dev Biol. 2003 Jun 15;258(2):464-74. (PMID: 12798302) Dev Biol. 1999 Jul 15;211(2):157-76. (PMID: 10395780) J Biol Chem. 1992 Sep 5;267(25):17624-30. (PMID: 1387638) Nat Methods. 2009 Dec;6(12):917-22. (PMID: 19915560) J Cell Biol. 2023 Nov 6;222(11):. (PMID: 37843812) PLoS Biol. 2021 Sep 7;19(9):e3001376. (PMID: 34491981) J Cell Sci. 2002 Sep 15;115(Pt 18):3557-64. (PMID: 12186942) Biol Reprod. 2008 Jun;78(6):1081-90. (PMID: 18322275) Biol Reprod. 2015 Apr;92(4):97. (PMID: 25761595) J Cell Biol. 2023 Nov 6;222(11):. (PMID: 37651121) Anim Sci J. 2013 Apr;84(4):359-68. (PMID: 23590511) Biochem Biophys Res Commun. 2016 Feb 26;471(1):219-25. (PMID: 26809094) Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3824-33. (PMID: 27325775) J Cell Biol. 2000 Oct 30;151(3):697-708. (PMID: 11062269) Hum Reprod. 2005 Nov;20(11):3053-61. (PMID: 16055456) Cell Motil Cytoskeleton. 2007 Nov;64(11):822-32. (PMID: 17685442) Nat Cell Biol. 2002 Dec;4(12):E281-3. (PMID: 12461532) Nat Methods. 2012 Jul;9(7):671-5. (PMID: 22930834) Dev Biol. 2008 Mar 15;315(2):257-79. (PMID: 18255053) J Biol Chem. 2012 Sep 14;287(38):31658-65. (PMID: 22778263) J Cell Sci. 2013 Dec 15;126(Pt 24):5714-24. (PMID: 24101727) Reproduction. 2005 Dec;130(6):813-23. (PMID: 16322541) J Cell Sci. 2001 Jun;114(Pt 12):2213-22. (PMID: 11493661) Dev Cell. 2007 Feb;12(2):301-8. (PMID: 17276346) |
| Grant Information: | ZIA HL006249 United States ImNIH Intramural NIH HHS |
| Contributed Indexing: | Keywords: Ca(2+) oscillations; actomyosin contraction; cytoplasmic streaming; fertilization; live imaging; meiosis II; mouse egg; second polar body extrusion; spindle dynamics |
| Substance Nomenclature: | SY7Q814VUP (Calcium) |
| Entry Date(s): | Date Created: 20250727 Date Completed: 20250819 Latest Revision: 20260726 |
| Update Code: | 20260726 |
| PubMed Central ID: | PMC12421433 |
| DOI: | 10.1016/j.cub.2025.06.073 |
| PMID: | 40713943 |
| Βάση Δεδομένων: | MEDLINE |
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