Academic Journal
Effect of intracanal medicaments on dentinal tubule penetration of root canal sealers: a confocal laser scanning microscopy study.
| Τίτλος: | Effect of intracanal medicaments on dentinal tubule penetration of root canal sealers: a confocal laser scanning microscopy study. |
|---|---|
| Συγγραφείς: | Bayram E; Faculty of Dentistry, Tokat Gaziosmanpasa University, Tokat, Türkiye., Bayram HM; Faculty of Dentistry, Tokat Gaziosmanpasa University, Tokat, Türkiye. melikealaca@yahoo.com. |
| Πηγή: | BMC oral health [BMC Oral Health] 2025 Jul 26; Vol. 25 (1), pp. 1253. Date of Electronic Publication: 2025 Jul 26. |
| Τύπος έκδοσης: | Journal Article; Comparative Study |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: BioMed Central Country of Publication: England NLM ID: 101088684 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6831 (Electronic) Linking ISSN: 14726831 NLM ISO Abbreviation: BMC Oral Health Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : BioMed Central, [2001- |
| Ιατρικοί όροι (MeSH): | Root Canal Irrigants*/pharmacology , Dentin*/drug effects , Dentin*/ultrastructure , Dental Pulp Cavity*/ultrastructure , Dental Pulp Cavity*/drug effects , Dentin Permeability*/drug effects , Root Canal Filling Materials*, Calcium Hydroxide/pharmacology ; Silicates/pharmacology ; Aluminum Compounds/pharmacology ; Calcium Compounds/pharmacology ; Anti-Bacterial Agents/pharmacology ; Oxides/pharmacology ; Metronidazole/pharmacology ; Ciprofloxacin/pharmacology ; Minocycline/pharmacology ; Humans ; Microscopy, Confocal ; Rhodamines ; Drug Combinations ; Epoxy Resins ; Fluorescent Dyes ; Fluorescein ; Materials Testing |
| Περίληψη: | Background: This study investigated the effect of three intracanal medicaments on the penetration ability of three different root canal sealers using confocal laser scanning microscopy (CLSM). Materials and Methods: 120 extracted single-rooted human teeth were used. The crowns were removed, and each canal was prepared. The teeth were divided into four groups (n = 30): three medicament groups (calcium hydroxide (CH), triple antibiotic paste (TAP), and double antibiotic paste [DAP]) and one control group. 0.1% Rhodamine B was incorporated. After three weeks, the medicaments were removed, and then canals were filled with one of the following sealers: AH Plus, MTA Fillapex, and TotalFill (n = 10). Fluorescein dye was added to the sealers. Horizontal sections (1 mm thick) were taken from each canal's coronal, middle, and apical portions. Samples were then viewed under CLSM. Statistical analysis was performed using Kruskal-Wallis and Mann-Whitney U tests. Results: TotalFill exhibited the highest dentinal tubule penetration values across all root levels and medicament groups, followed by AH Plus and MTA Fillapex. However, these differences were not statistically significant (p > 0.05). All sealers showed greater penetration in the coronal third compared to the apical third. Conclusions: Although intracanal medicaments did not significantly affect sealer penetration, TotalFill demonstrated more consistent penetration patterns regardless of the medicament used. (© 2025. The Author(s).) |
| Competing Interests: | Declarations. Ethics approval and consent to participate: All procedures performed in studies involving human biological material were by the ethical standards of the institutional and national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This study was approved by the ethics committee of Tokat Gaziosmanpaşa University’s Faculty of Medicine project number 15- KAEK-071. The informed consent form was obtained. Consent for publication: Not applicable to this study. Competing interests: The authors declare no competing interests. |
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| Contributed Indexing: | Keywords: AH plus; Confocal microscopy; Dentin penetration; Intracanal medicament; Root Canal sealer |
| Substance Nomenclature: | 0 (Root Canal Filling Materials) PF5DZW74VN (Calcium Hydroxide) 0 (Silicates) 0 (Root Canal Irrigants) 0 (Rhodamines) K7G5SCF8IL (rhodamine B) 0 (Drug Combinations) 0 (Aluminum Compounds) 0 (mineral trioxide aggregate) 0 (Calcium Compounds) 0 (Anti-Bacterial Agents) 0 (epoxy resin-based root canal sealer) 0 (Oxides) 0 (Epoxy Resins) 140QMO216E (Metronidazole) 0 (Fluorescent Dyes) 5E8K9I0O4U (Ciprofloxacin) TPY09G7XIR (Fluorescein) FYY3R43WGO (Minocycline) |
| Entry Date(s): | Date Created: 20250727 Date Completed: 20250727 Latest Revision: 20250731 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12297755 |
| DOI: | 10.1186/s12903-025-06657-0 |
| PMID: | 40713572 |
| Βάση Δεδομένων: | MEDLINE |
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