Academic Journal
Bio-Designed Carboxymethyl Chitosan/Polyvinyl Alcohol Hydrogel Containing Alpha-Tocopherol as a Three-Dimensional Wound Dressing, In Vitro and In Vivo Study.
| Τίτλος: | Bio-Designed Carboxymethyl Chitosan/Polyvinyl Alcohol Hydrogel Containing Alpha-Tocopherol as a Three-Dimensional Wound Dressing, In Vitro and In Vivo Study. |
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| Συγγραφείς: | Hanjani GN; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran., Irani S; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran., Sharifi F; Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.; Soft Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Central Tehran Branch, Islamic Azad University, Tehran, Iran., Atyabi SM; Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran. |
| Πηγή: | Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2026 Feb; Vol. 73 (1), pp. 138-151. Date of Electronic Publication: 2025 Jul 10. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 8609465 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1470-8744 (Electronic) Linking ISSN: 08854513 NLM ISO Abbreviation: Biotechnol Appl Biochem Subsets: MEDLINE |
| Imprint Name(s): | Publication: Jan. 2011- : Malden : Wiley-Blackwell Original Publication: San Diego : Academic Press, [cl986]- |
| Ιατρικοί όροι (MeSH): | Chitosan*/analogs & derivatives , Chitosan*/chemistry , Chitosan*/pharmacology , Polyvinyl Alcohol*/chemistry , Polyvinyl Alcohol*/pharmacology , Wound Healing*/drug effects , Hydrogels*/chemistry , Hydrogels*/pharmacology , alpha-Tocopherol*/chemistry , alpha-Tocopherol*/pharmacology , Anti-Bacterial Agents*/pharmacology , Anti-Bacterial Agents*/chemistry , Bandages*, Staphylococcus aureus/drug effects ; Escherichia coli/drug effects ; Animals ; Rats ; Humans ; Rats, Sprague-Dawley ; Male |
| Περίληψη: | Chitosan-based hydrogels have highly attractive potential for designing wound dressing production. To promote wound healing, it is admirable to develop three-dimensional (3D) multi-biological functional polyvinyl alcohol/carboxymethyl chitosan (PVA/CMC) hydrogel. A 3D biocompatible PVA/CMC hydrogel was prepared using a freeze-drying method under both sterile and unsterile conditions, without the use of a chemical cross-linker. The alpha-tocopherol (αT) and glycerol (Gly) loaded into the hydrogel resulted in stability, porosity, and a controlled release profile coordinated with the degradation rate of the hydrogel. The PVA/CMC + αT/Gly hydrogel displayed facilitates cell attachment and cellular migration in vitro evaluations. The prepared hydrogels notably suppressed Escherichia coli and Staphylococcus aureus activities, correlating with loaded αT concentrations (200 and 400 U). In addition, the PVA/CMC + αT (200 U)/Gly10% and PVA/CMC + αT (400 U)/Gly10% hydrogels examined wound healing in full-thickness excisional wounds in rat models. The in vivo results indicated wound closure (αT-200 U: 78.7% and αT-400 U: 90.9% after 14 days), upregulating the gene expression (CYTOKERATIN, COLLAGEN TYPE Ι, and VEGF), and increasing protein levels (Pan-cytokeratin: 34.98% and VEGF: 41.72%). These findings highlight the porous structure of the PVA/CMC + αT (400 U)/Gly10% hydrogel as a promising novel wound dressing candidate with pro-regenerative properties. (© 2025 International Union of Biochemistry and Molecular Biology, Inc.) |
| References: | E. Serag, A. M. Eltawila, E. M. Salem, A. El‐Maghraby, and A. M. Abd El‐Aziz, “Development of an Innovative Cylindrical Carbon Nanofiber/Gelatin‐Polycaprolactone Hydrogel Scaffold for Enhanced Bone Regeneration,” International Journal of Biological Macromolecules 306 (2025): 141250. X. Li, X. Hou, A. Yang, D. Li, and F. Zhou, “Antibacterial Methacrylamide Chitosan‐Modified Viscose/Poly (ε‐Caprolactone) Hydrogel Membranes for Wound Healing,” Cellulose 18 (2024): 11035–11050. B. F. Far, M. R. Naimi‐Jamal, M. Jahanbakhshi, H. Rostamani, M. Karimi, and S. Keihankhadiv, “Synthesis and Characterization of Chitosan/Collagen/Polycaprolactone Hydrogel Films With Enhanced Biocompatibility and Hydrophilicity for Artificial Tendon Applications,” International Journal of Biological Macromolecules 253 (2023): 127448. A. Moeini, P. Pedram, P. Makvandi, M. Malinconico, and G. G. d'Ayala, “Wound Healing and Antimicrobial Effect of Active Secondary Metabolites in Chitosan‐Based Wound Dressings: A Review,” Carbohydrate Polymers 233 (2020): 115839. W. Guo, X. Ding, H. Zhang, et al., “Recent Advances of Chitosan‐Based Hydrogels for Skin‐Wound Dressings,” Gels 10, no. 3 (2024): 175. J. Zhao, P. Qiu, Y. Wang, et al., “Chitosan‐Based Hydrogel Wound Dressing: From Mechanism to Applications, a Review,” International Journal of Biological Macromolecules 244 (2023): 125250. X. F. Liu, Y. L. Guan, D. Z. Yang, Z. Li, and K. De Yao, “Antibacterial Action of Chitosan and Carboxymethylated Chitosan,” Journal of Applied Polymer Science 79, no. 7 (2001): 1324–1335. N. Chaiwong, P. Leelapornpisid, K. Jantanasakulwong, et al., “Antioxidant and Moisturizing Properties of Carboxymethyl Chitosan With Different Molecular Weights,” Polymers 12, no. 7 (2020): 1445. A. G. Kanani, S. H. Bahrami, H. A. Taftei, S. Rabbani, and M. Sotoudeh, “Effect of Chitosan‐Polyvinyl Alcohol Blend Nanofibrous Web on the Healing of Excision and Incision Full Thickness Wounds,” IET Nanobiotechnology 4, no. 4 (2010): 109–117. M. Lazaridou, D. N. Bikiaris, and D. A. Lamprou, “3D Bioprinted Chitosan‐Based Hydrogel Scaffolds in Tissue Engineering and Localised Drug Delivery,” Pharmaceutics 14, no. 9 (2022): 1978. A. Akbari, S. Rabbani, S. Irani, et al., “In Vitro and In Vivo Study of Carboxymethyl Chitosan/Polyvinyl Alcohol for Wound Dressing Application,” Journal of Applied Polymer Science 139, no. 10 (2022): 51764. A. Ehterami, M. Salehi, S. Farzamfar, et al., “Chitosan/Alginate Hydrogels Containing Alpha‐Tocopherol for Wound Healing in Rat Model,” Journal of Drug Delivery Science and Technology 51 (2019): 204–213. Q. Liao, E. J. Kim, Y. Tang, et al., “Rational Design of Hyper‐Crosslinked Polymers for Biomedical Applications,” Journal of Polymer Science 62, no. 8 (2024): 1517–1535. L. Zhao, Y. Zhou, J. Zhang, H. Liang, X. Chen, and H. Tan, “Natural Polymer‐Based Hydrogels: From Polymer to Biomedical Applications,” Pharmaceutics 15, no. 10 (2023): 2514. A. J. Capel, R. P. Rimington, M. P. Lewis, and S. D. Christie, “3D Printing for Chemical, Pharmaceutical and Biological Applications,” Nature Reviews Chemistry 2, no. 12 (2018): 422–436. C. Chircov, I. T. Bejenaru, A. I. Nicoară, A. C. Bîrcă, O. C. Oprea, and B. Tihăuan, “Chitosan‐Dextran‐Glycerol Hydrogels Loaded With Iron Oxide Nanoparticles for Wound Dressing Applications,” Pharmaceutics 14, no. 12 (2022): 2620. A. Das, S. Bhattacharyya, R. Uppaluri, and C. Das, “Optimality of Poly‐Vinyl Alcohol/Starch/Glycerol/Citric Acid in Wound Dressing Applicable Composite Films,” International Journal of Biological Macromolecules 155 (2020): 260–272. A. Das, R. Uppaluri, and C. Das, “Feasibility of Poly‐Vinyl Alcohol/Starch/Glycerol/Citric Acid Composite Films for Wound Dressing Applications,” International Journal of Biological Macromolecules 131 (2019): 998–1007. M. Bayindir Bilgic, N. T. Lacin, H. Berber, and B. Mansuroglu, “In Vitro Evaluation of Alpha‐Tocopherol Loaded Carboxymethylcellulose Chitosan Copolymers as Wound Dressing Materials,” Materials Technology 34, no. 7 (2019): 386–393. B. Balakrishnan, M. Mohanty, P. R. Umashankar, and A. Jayakrishnan, “Evaluation of an In Situ Forming Hydrogel Wound Dressing Based on Oxidized Alginate and Gelatin,” Biomaterials 26, no. 32 (2005): 6335–6342. M. J. Moura, H. Faneca, M. P. Lima, M. H. Gil, and M. M. Figueiredo, “In Situ Forming Chitosan Hydrogels Prepared via Ionic/Covalent Co‐Cross‐Linking,” Biomacromolecules 12, no. 9 (2011): 3275–3284. S. Maiz‐Fernández, O. Guaresti, L. Pérez‐Álvarez, et al., “β‐Glycerol Phosphate/Genipin Chitosan Hydrogels: A Comparative Study of Their Properties and Diclofenac Delivery,” Carbohydrate Polymers 248 (2020): 116811. H. Liu, C. Wang, C. Li, et al., “A Functional Chitosan‐Based Hydrogel as a Wound Dressing and Drug Delivery System in the Treatment of Wound Healing,” RSC Advances 8, no. 14 (2018): 7533–7549. S. R. Chakavala, N. G. Patel, N. V. Pate, V. T. Thakkar, K. V. Patel, and T. R. Gandhi, “Development and In Vivo Evaluation of Silver Sulfadiazine Loaded Hydrogel Consisting Polyvinyl Alcohol and Chitosan for Severe Burns,” Journal of Pharmacy & Bioallied Sciences 4, no. S1 (2012): S54–S56. S. A. Anjum, A. Alam, and M. S. Gupta, “Development of Antimicrobial and Scar Preventive Chitosan Hydrogel Wound Dressings,” International Journal of Biological Macromolecules 508 (2016): 92–101. M. Zhang, S. Chen, L. Zhong, B. Wang, H. Wang, and F. Hong, “Zn2+‐Loaded TOBC Nanofiber‐Reinforced Biomimetic Calcium Alginate Hydrogel for Antibacterial Wound Dressing,” International Journal of Biological Macromolecules 143 (2020): 235–242. R. I. Freshney, Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications (John Wiley & Sons, 2015). M. W. Pfaffl, “A New Mathematical Model for Relative Quantification In Real‐Time RT‐PCR,” Nucleic Acids Research 29, no. 9 (2001): e45. X. Feng, X. Hou, C. Cui, et al., “Mechanical and Antibacterial Properties of Tannic Acid‐Encapsulated Carboxymethyl Chitosan/Polyvinyl Alcohol Hydrogels,” Engineered Regeneration 2 (2021): 57–62. F. Sharifi, S. M. Atyabi, D. Norouzian, M. Zandi, S. Irani, and H. Bakhshi, “Polycaprolactone/Carboxymethyl Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering Application,” International Journal of Biological Macromolecules 115 (2018): 243–248. E. Massarelli, D. Silva, A. F. R. Pimenta, et al., “Polyvinyl Alcohol/Chitosan Wound Dressings Loaded With Antiseptics,” International Journal of Pharmaceutics 593 (2021): 120110. A. S. Giz, M. Berberoglu, S. Bener, et al., “A Detailed Investigation of the Effect of Calcium Crosslinking and Glycerol Plasticizing on the Physical Properties of Alginate Films,” International Journal of Biological Macromolecules 148 (2020): 49–55. S. M. Nasef, E. E. Khozemy, E. A. Kamoun, and H. El‐Gendi, “Gamma Radiation‐Induced Crosslinked Composite Membranes Based on Polyvinyl Alcohol/Chitosan/AgNO3/Vitamin E for Biomedical Applications,” International Journal of Biological Macromolecules 137 (2019): 878–885. A. Kabirkoohian, H. Bakhshi, S. Irani, and F. Sharifi, “Chemical Immobilization of Carboxymethyl Chitosan on Polycaprolactone Nanofibers as Osteochondral Scaffolds,” Applied Biochemistry and Biotechnology 195 (2022): 1–12. H. S. Kim, X. Sun, J. H. Lee, H. W. Kim, X. Fu, and K. W. Leong, “Advanced Drug Delivery Systems and Artificial Skin Grafts for Skin Wound Healing,” Advanced Drug Delivery Reviews 146 (2019): 209–239. T. Hashimoto, K. Kojima, and Y. Tamada, “Gene Expression Advances Skin Reconstruction and Wound Repair Better on Silk Fibroin‐Based Materials Than on Collagen‐Based Materials,” Materialia 9 (2020): 100519. A. Li, B. Ma, S. Hua, et al., “Chitosan‐Based Injectable Hydrogel With Multifunction for Wound Healing: A Critical Review,” Carbohydrate Polymer 333 (2024): 121952. F. Sharifi, M. Hasani, S. M. Atyabi, et al., “Mesenchymal Stem Cells Encapsulation in Chitosan and Carboxymethyl Chitosan Hydrogels to Enhance Osteo‐Differentiation,” Molecular Biology Reports 49 (2022): 12063–12075. Q. Ding, X. Liu, X. Liu, et al., “Polyvinyl Alcohol/Carboxymethyl Chitosan‐Based Hydrogels Loaded With Taxifolin Liposomes Promote Diabetic Wound Healing by Inhibiting Inflammation and Regulating Autophagy,” International Journal of Biological Macromolecules 263 (2024): 130226. R. F. Pereira, C. C. Barrias, P. L. Granja, and P. J. Bartolo, “Advanced Biofabrication Strategies for Skin Regeneration and Repair,” Nanomedicine 8, no. 4 (2013): 603–621. N. Bhattarai, J. Gunn, and M. Zhang, “Chitosan‐Based Hydrogels for Controlled, Localized Drug Delivery,” Advanced Drug Delivery Reviews 62, no. 1 (2010): 83–99. S. Supper, N. Anton, N. Seidel, M. Riemenschnitter, C. Schoch, and T. Vandamme, “Rheological Study of Chitosan/Polyol‐Phosphate Systems: Influence of the Polyol Part on the Thermo‐Induced Gelation Mechanism,” Langmuir 29, no. 32 (2013): 10229–10237. J. M. Zingg, “Vitamin E: Regulatory Role on Signal Transduction,” IUBMB Life 71, no. 4 (2019): 456–478. A. Azzi, “Many Tocopherols, One Vitamin E,” Molecular Aspects of Medicine 61 (2018): 92–103. C. L. Huang, Y. B. Chen, Y. L. Lo, and Y. H. Lin, “Development of Chitosan/β‐Glycerophosphate/Glycerol Hydrogel as a Thermosensitive Coupling Agent,” Carbohydrate Polymers 147 (2016): 409–414. F. Sharifi, S. M. Atyabi, S. Irani, and H. Bakhshi, “Bone Morphogenic Protein‐2 Immobilization by Cold Atmospheric Plasma to Enhance the Osteoinductivity of Carboxymethyl Chitosan‐Based Nanofibers,” Carbohydrate Polymers 231 (2020): 115681. A. K. Singh, M. R. Sen, S. Anupurba, and P. Bhattacharya, “Antibiotic Sensitivity Pattern of the Bacteria Isolated From Nosocomial Infections in ICU,” The Journal of Communicable Diseases 34, no. 4 (2002): 257–263. S. Yoshizawa, “Structural Origins of Gentamicin Antibiotic Action,” EMBO Journal 17, no. 22 (1998): 6437–6448. Z. Hui, X. Pan, Y. Li, et al., “Dynamic Carboxymethyl Chitosan Prodrug Hydrogel Precisely Mediates Robust Therapy on Wound Infection,” International Journal of Biological Macromolecules 260 (2024): 129529. M. B. Witte and A. Barbul, “General Principles of Wound Healing,” Surgical Clinical of North America 77, no. 3 (1997): 509–528. L. Mohammadzadeh, R. Rahbarghazi, R. Salehi, and M. Mahkam, “A Novel Egg‐Shell Membrane Based Hybrid Nanofibrous Scaffold for Cutaneous Tissue Engineering,” Journal of Biological Engineering 13, no. 1 (2019): 1–15. B. Kleineidam, M. Nokhbehsaim, J. Deschner, and G. Wahl, “Effect of Cold Plasma on Periodontal Wound Healing—An In Vitro Study,” Clinical Oral Investigations 23, no. 4 (2019): 1941–1950. M. Zhang, M. Yang, M. W. Woo, Y. Li, W. Han, and X. Dang, “High‐Mechanical Strength Carboxymethyl Chitosan‐Based Hydrogel Film for Antibacterial Wound Dressing,” Carbohydrate Polymers 256 (2021): 117590. A. Patruno, A. Ferrone, E. Costantini, et al., “Extremely Low‐Frequency Electromagnetic Fields Accelerates Wound Healing Modulating MMP ‐9 and Inflammatory Cytokines,” Cell Proliferation 51, no. 2 (2018): 12432. A. A. Shefa, J. Amirian, H. J. Kang, et al., “In Vitro and In Vivo Evaluation of Effectiveness of a Novel TEMPO‐Oxidized Cellulose Nanofiber‐Silk Fibroin Scaffold in Wound Healing,” Carbohydrate Polymers 177 (2017): 284–296. A. Satish and P. S. Korrapati, “Fabrication of a Triiodothyronine Incorporated Nanofibrous Biomaterial: Its Implications on Wound Healing,” RSC Advances 5, no. 102 (2015): 83773–83780. M. A. Norouzi, M. Montazer, T. Harifi, and P. Karimi, “Flower Buds Like PVA/ZnO Composite Nanofibers Assembly: Antibacterial, in Vivo Wound Healing, Cytotoxicity and Histological Studies,” Polymer Testing 93 (2021): 106914. G. Karsenty and R.‐W. Park, “Regulation of Type I Collagen Genes Expression,” International Review Immunology 12, no. 2–4 (1995): 177–185. G. A. Di Luullo, S. M. Sweeney, J. Korkko, L. Ala‐Kokko, and J. D. San Ntonio, “Mapping the Ligand‐Binding Site and Disease‐Associated Mutations on the Most Abundent Protein in the Human, Type 1 Collagen,” Journal of Biological Chemistry 277, no. 6 (2002): 4223–4231. D. Harper, A. Young, and C.‐E. Mcnaught, “The Physiology of Wound Healing,” Surgery (Oxford) 32, no. 9 (2014): 445–450. A. P. Larsson, K. Briheim, V. Hanna et al., “Transplantation of Autologous Cells and Porous Gelatin Microcarriers to Promote Wound Healing,” Burns 47 (2021): 601–610. L. Roshangar, J. S. Rad, R. Kheirjou, and A. F. Khosroshahi, “Using 3D‐Bioprinting Scaffold Loaded With Adipose‐Derived Stem Cells to Burns Wound Healing,” Journal of Tissue Engineering and Regenerative Medicine 15, no. 6 (2021): 546–555. M. T. Khorasani, A. Joorabloo, H. Adeli, P. B. Milan, and M. Amoupour, “Enhanced Antimicrobial and Full‐Thickness Wound Healing Efficiency of Hydrogels Loaded With Heparinized ZnO Nanoparticles: In Vitro and In Vivo Evaluation,” International Journal of Biological Macromolecules 166 (2021): 200–212. |
| Contributed Indexing: | Keywords: alpha‐tocopherol (αT); carboxymethyl chitosan; hydrogel; polyvinyl alcohol; wound dressing |
| Substance Nomenclature: | 9012-76-4 (Chitosan) 9002-89-5 (Polyvinyl Alcohol) 0 (carboxymethyl-chitosan) 0 (Hydrogels) H4N855PNZ1 (alpha-Tocopherol) 0 (polyvinyl alcohol hydrogel) 0 (Anti-Bacterial Agents) |
| Entry Date(s): | Date Created: 20250711 Date Completed: 20260213 Latest Revision: 20260213 |
| Update Code: | 20260213 |
| DOI: | 10.1002/bab.70010 |
| PMID: | 40641222 |
| Βάση Δεδομένων: | MEDLINE |
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