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An Overview of Epitopes and Methods for Antibody Epitope Mapping.

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Title: An Overview of Epitopes and Methods for Antibody Epitope Mapping.
Authors: Nilvebrant J; School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden., Berndt Thalén N; School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden., Rockberg J; School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden. johanr@biotech.kth.se., Malm M; School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden. magdalena.malm@biotech.kth.se.
Source: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2937, pp. 1-11.
Publication Type: Journal Article; Review
Language: English
Journal Info: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
Imprint Name(s): Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press
MeSH Terms: Epitope Mapping*/methods , Epitopes*/immunology , Epitopes*/chemistry , Epitopes*/genetics , Antibodies*/immunology , Antibodies*/chemistry, Computational Biology/methods ; Antigens/immunology ; Antigens/chemistry ; Humans ; Animals
Abstract: Antibodies are protein molecules indispensable for many therapeutic, diagnostic, and research purposes due to their exquisite ability to selectively recognize and bind a given antigen. The particular area of the antigen recognized by the antibody is called the epitope, and mapping of such epitopes can provide important mechanistic insights and indicate for what applications an antibody might be useful. Even though the nature of epitopes can be complex, epitopes in protein antigens are broadly grouped into linear or discontinuous epitopes depending on the positioning of the epitope residues in the antigen sequence and the requirement of structure. Specialized methods for mapping the two different classes of epitopes have been developed. While different in their detail, all of the experimental methods rely on assessing the binding of the antibody to the antigen or a set of antigen mimics. Classical epitope mapping methods, utilizing truncated proteins, small numbers of synthesized peptides, and structural analyses of antibody-antigen complexes, have been significantly refined. Current state-of-the-art methods involve combinations of mutational scanning, protein display, and high throughput screening in conjunction with bioinformatic analyses of large datasets. In line with the significant development of in silico protein structure prediction in recent years, computerized approaches for epitope prediction have also matured considerably.
(© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
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Contributed Indexing: Keywords: Antibody; Antigen; Epitope; Epitope mapping; Peptide; Prediction; Structural biology
Substance Nomenclature: 0 (Epitopes)
0 (Antibodies)
0 (Antigens)
Entry Date(s): Date Created: 20250702 Date Completed: 20250702 Latest Revision: 20250702
Update Code: 20260130
DOI: 10.1007/978-1-0716-4591-8_1
PMID: 40593411
Database: MEDLINE
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  Data: Antibodies are protein molecules indispensable for many therapeutic, diagnostic, and research purposes due to their exquisite ability to selectively recognize and bind a given antigen. The particular area of the antigen recognized by the antibody is called the epitope, and mapping of such epitopes can provide important mechanistic insights and indicate for what applications an antibody might be useful. Even though the nature of epitopes can be complex, epitopes in protein antigens are broadly grouped into linear or discontinuous epitopes depending on the positioning of the epitope residues in the antigen sequence and the requirement of structure. Specialized methods for mapping the two different classes of epitopes have been developed. While different in their detail, all of the experimental methods rely on assessing the binding of the antibody to the antigen or a set of antigen mimics. Classical epitope mapping methods, utilizing truncated proteins, small numbers of synthesized peptides, and structural analyses of antibody-antigen complexes, have been significantly refined. Current state-of-the-art methods involve combinations of mutational scanning, protein display, and high throughput screening in conjunction with bioinformatic analyses of large datasets. In line with the significant development of in silico protein structure prediction in recent years, computerized approaches for epitope prediction have also matured considerably.&lt;br /&gt; (&#169; 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
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  Label: References
  Group: RefInfo
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– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: &lt;i&gt;Keywords: &lt;/i&gt;Antibody; Antigen; Epitope; Epitope mapping; Peptide; Prediction; Structural biology
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 0 (Epitopes)&lt;br /&gt;0 (Antibodies)&lt;br /&gt;0 (Antigens)
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: &lt;i&gt;Date Created: &lt;/i&gt;20250702 &lt;i&gt;Date Completed: &lt;/i&gt;20250702 &lt;i&gt;Latest Revision: &lt;/i&gt;20250702
– Name: DateUpdate
  Label: Update Code
  Group: Date
  Data: 20260130
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1007/978-1-0716-4591-8_1
– Name: AN
  Label: PMID
  Group: ID
  Data: 40593411
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=40593411
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/978-1-0716-4591-8_1
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: 1
    Subjects:
      – SubjectFull: Computational Biology methods
        Type: general
      – SubjectFull: Antigens immunology
        Type: general
      – SubjectFull: Antigens chemistry
        Type: general
      – SubjectFull: Humans
        Type: general
      – SubjectFull: Animals
        Type: general
      – SubjectFull: Epitope Mapping methods
        Type: general
      – SubjectFull: Epitopes immunology
        Type: general
      – SubjectFull: Epitopes chemistry
        Type: general
      – SubjectFull: Epitopes genetics
        Type: general
      – SubjectFull: Antibodies immunology
        Type: general
      – SubjectFull: Antibodies chemistry
        Type: general
    Titles:
      – TitleFull: An Overview of Epitopes and Methods for Antibody Epitope Mapping.
        Type: main
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            NameFull: Nilvebrant J
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            NameFull: Berndt Thalén N
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            NameFull: Rockberg J
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            NameFull: Malm M
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          Dates:
            – D: 01
              M: 01
              Text: 2025
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-electronic
              Value: 1940-6029
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            – Type: volume
              Value: 2937
          Titles:
            – TitleFull: Methods in molecular biology (Clifton, N.J.)
              Type: main
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