Academic Journal
Cellular Senescence in Human Chondrocytes in Relation to Osteoarthritis.
| Τίτλος: | Cellular Senescence in Human Chondrocytes in Relation to Osteoarthritis. |
|---|---|
| Συγγραφείς: | Qiang Y; Center for Single-Cell Omics, School of Public Health, School of Medicine, Shanghai Jiao Tong University, Shanghai, China., Zheng C; Center for Single-Cell Omics, School of Public Health, School of Medicine, Shanghai Jiao Tong University, Shanghai, China., Maslov AY; Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA., Lu Z; Center for Single-Cell Omics, School of Public Health, School of Medicine, Shanghai Jiao Tong University, Shanghai, China., Zhou M; Center for Single-Cell Omics, School of Public Health, School of Medicine, Shanghai Jiao Tong University, Shanghai, China., Gao J; Department of Orthopaedics, Shanghai Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China., Ren P; Center for Single-Cell Omics, School of Public Health, School of Medicine, Shanghai Jiao Tong University, Shanghai, China., Pang Y; Department of Orthopaedics, Shanghai Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China., Vijg J; Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA. |
| Πηγή: | Cartilage [Cartilage] 2026 Sep; Vol. 17 (3), pp. 762-770. Date of Electronic Publication: 2025 Jun 16. |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Sage Country of Publication: United States NLM ID: 101518378 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1947-6043 (Electronic) Linking ISSN: 19476035 NLM ISO Abbreviation: Cartilage Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Los Angeles : Sage |
| Ιατρικοί όροι (MeSH): | Cellular Senescence*/physiology , Cellular Senescence*/drug effects , Chondrocytes*/drug effects , Chondrocytes*/pathology , Chondrocytes*/metabolism , Cartilage, Articular*/pathology , Cartilage, Articular*/cytology , Cartilage, Articular*/metabolism , Osteoarthritis*/pathology, Apoptosis/drug effects ; bcl-2-Associated X Protein/metabolism ; Cyclin-Dependent Kinase Inhibitor p21/metabolism ; beta-Galactosidase/metabolism ; Cyclin-Dependent Kinase Inhibitor p16/metabolism ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Humans ; Female ; Cells, Cultured ; Aged ; Middle Aged ; Male |
| Περίληψη: | IntroductionCellular senescence, i.e., a state of permanent cessation of cell division, is a hallmark of aging and has been associated with age-related diseases, most notably osteoarthritis (OA). Here we assessed senescence in chondrocytes, first in vitro after treatment with the mutagens N-ethyl-N-nitrosourea (ENU) or bleomycin, and then in vivo in cartilage samples from OA patients and control subjects.MethodsCellular senescence in cultured chondrocytes treated with mutagens was assessed by senescence-associated β-galactosidase (SA-β-gal) staining and by evaluating the expression levels of p16 and p21. Cellular senescence in human hip cartilage chondrocytes from OA patients or non-OA controls was similarly evaluated. Apoptosis in vitro was measured by the Bcl-2/Bax expression ratio, and in vivo by both TUNEL assay and the Bcl-2/Bax ratio.ResultsIn human articular cartilage, senescent cells were found to be significantly elevated in OA lesions of patients as compared with normal cartilage of non-OA control subjects. In vitro, senescence was observed in bleomycin-treated chondrocytes, but not in ENU-treated cells.ConclusionsOur findings demonstrate that cellular senescence is associated with the pathogenesis of OA, with DNA damage and mutations as potential contributing factors in OA-associated senescence. |
| Competing Interests: | Declaration of Conflicting InterestsThe author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: J.V. and A.Y.M. are co-founders of Singulomics Corp and MutagenTech Inc. The remaining authors declare no competing interests. |
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| Grant Information: | P01 AG047200 United States AG NIA NIH HHS |
| Contributed Indexing: | Keywords: age-related disease; aging; cellular senescence; chondrocytes; osteoarthritis |
| Substance Nomenclature: | 0 (bcl-2-Associated X Protein) 0 (Cyclin-Dependent Kinase Inhibitor p21) EC 3.2.1.23 (beta-Galactosidase) 0 (Cyclin-Dependent Kinase Inhibitor p16) 0 (Proto-Oncogene Proteins c-bcl-2) 0 (BAX protein, human) |
| Entry Date(s): | Date Created: 20250616 Date Completed: 20260814 Latest Revision: 20260924 |
| Update Code: | 20260924 |
| PubMed Central ID: | PMC12170564 |
| DOI: | 10.1177/19476035251344875 |
| PMID: | 40521922 |
| Βάση Δεδομένων: | MEDLINE |
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