Academic Journal
Intermediate Filament Protein BFSP1 Maintains Oocyte Asymmetric Division by Modulating Spindle Length.
| Τίτλος: | Intermediate Filament Protein BFSP1 Maintains Oocyte Asymmetric Division by Modulating Spindle Length. |
|---|---|
| Συγγραφείς: | Li Y; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China., Zhang H; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China., Zeng W; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China., Miao Y; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China., Sun S; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China., Zhang Y; College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China., Xiong B; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.; College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China. |
| Πηγή: | Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2025 Jul; Vol. 12 (28), pp. e2504066. Date of Electronic Publication: 2025 May 11. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: WILEY-VCH Country of Publication: Germany NLM ID: 101664569 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2198-3844 (Electronic) Linking ISSN: 21983844 NLM ISO Abbreviation: Adv Sci (Weinh) Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Weinheim : WILEY-VCH, [2014]- |
| Ιατρικοί όροι (MeSH): | Oocytes*/metabolism , Oocytes*/cytology , Spindle Apparatus*/metabolism , Microtubule-Associated Proteins*/metabolism , Microtubule-Associated Proteins*/genetics , Intermediate Filament Proteins*/metabolism , Intermediate Filament Proteins*/genetics, Meiosis/physiology ; Intermediate Filaments/metabolism ; Microtubules/metabolism ; Animals ; Female ; Mice |
| Περίληψη: | The cytoskeleton is composed of microtubules, microfilaments, and intermediate filaments in cells. While the functions of microtubules and microfilaments have been well elucidated, the roles of intermediate filaments and associated proteins remain largely unknown, especially in meiosis. BFSP1 is an intermediate filament protein mainly expressed in the eye lens to play important roles in the development of congenital cataract. Here, we document that BFSP1 functions as a spindle regulator to drive the oocyte asymmetric division. Specifically, we found that BFSP1 distributed on the spindle apparatus during oocyte meiotic maturation. Depletion of BFSP1 resulted in symmetric division of oocytes, accompanied by the formation of elongated spindles at metaphase I and anaphase/telophase I stages. In addition, immunoprecipitation combined with mass spectrometry analysis identified MAP1B, a microtubule-associated protein, as an interacting partner of BFSP1. Depletion or mutation of MAP1B phenocopied the meiotic defects observed in BFSP1-depleted oocytes, and expression of exogenous MAP1B-EGFP in BFSP1-depleted oocytes recovered the spindle length and asymmetric division. We further determined that BFSP1 recruited molecular chaperone HSP90α on the spindle to stabilize MAP1B, thereby controlling the spindle length. To sum up, our findings reveal a unique meiotic role for BFSP1 in the regulation of spindle dynamics and oocyte asymmetric division. (© 2025 The Author(s). Advanced Science published by Wiley‐VCH GmbH.) |
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| Grant Information: | LZ25C170003 Zhejiang Provincial Natural Science Foundation; 2023YFD1300502 National Key Research and Development Program of China; KYCX24_0993 Postgraduate Research & Practice Innovation Program of Jiangsu Province |
| Contributed Indexing: | Keywords: BFSP1; asymmetric division; intermediate filament protein; oocyte meiosis; spindle length |
| Substance Nomenclature: | 0 (Microtubule-Associated Proteins) 0 (microtubule-associated protein 1B) 0 (Intermediate Filament Proteins) |
| Entry Date(s): | Date Created: 20250511 Date Completed: 20250724 Latest Revision: 20250730 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12302563 |
| DOI: | 10.1002/advs.202504066 |
| PMID: | 40349178 |
| Βάση Δεδομένων: | MEDLINE |
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