Academic Journal
Urease in acetogenic Lachnospiraceae drives urea carbon salvage in SCFA pools.
| Τίτλος: | Urease in acetogenic Lachnospiraceae drives urea carbon salvage in SCFA pools. |
|---|---|
| Συγγραφείς: | Firth IJ; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada., Sim MAR; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada., Fitzgerald BG; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada., Moore AE; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada., Pittao CR; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada., Gianetto-Hill C; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada., Hess S; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada., Sweeney AR; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada., Allen-Vercoe E; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada., Sorbara MT; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada. |
| Πηγή: | Gut microbes [Gut Microbes] 2025 Dec; Vol. 17 (1), pp. 2492376. Date of Electronic Publication: 2025 Apr 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101495343 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1949-0984 (Electronic) Linking ISSN: 19490976 NLM ISO Abbreviation: Gut Microbes Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2015- : Philadelphia, PA : Taylor & Francis Original Publication: Austin, Tex. : Landes Bioscience |
| Ιατρικοί όροι (MeSH): | Urease*/metabolism , Urease*/genetics , Urea*/metabolism , Fatty Acids, Volatile*/metabolism , Carbon*/metabolism , Eubacteriales*/enzymology , Eubacteriales*/metabolism , Eubacteriales*/genetics , Eubacteriales*/growth & development , Bacterial Proteins*/metabolism , Bacterial Proteins*/genetics, Colon/microbiology ; Gastrointestinal Microbiome ; Animals ; Mice ; Humans ; Hydrogen-Ion Concentration |
| Περίληψη: | The gut microbiota produces short-chain fatty acids (SCFA) and acidifies the proximal colon which inhibits enteric pathogens. However, for many microbiota constituents, how they themselves resist these stresses is unknown. The anaerobic Lachnospiraceae family, which includes the acetogenic genus Blautia, produce SCFA, are genomically diverse, and vary in their capacity to acidify culture media. Here, we investigated how Lachnospiraceae tolerate pH stress and found that subunits of urease were associated with acidification in a random forest model. Urease cleaves urea into ammonia and carbon dioxide, however the role of urease in the physiology of Lachnospiraceae is unknown. We demonstrate that urease-encoding Blautia show urea-dependent changes in SCFA production, acidification, growth, and, strikingly, urease encoding Blautia directly incorporate the carbon from urea into SCFAs. In contrast, ureolytic Klebsiella pneumoniae or Proteus mirabilis do not show the same urea-dependency or carbon salvage. In agreement, the combination of urease and acetogenesis functions is rare in gut taxa. We find that Lachnospiraceae urease and acetogenesis genes can be co-expressed in healthy individuals and colonization of mice with a ureolytic Blautia reduces urea availability in colon contents demonstrating Blautia urease activity in vivo. In human and mouse microbial communities, the acetogenic recycling of urea carbon into acetate by Blautia leads to the incorporation of urea carbon into butyrate indicating carbon salvage into broader metabolite pools. Altogether, this shows that urea plays a central role in the physiology of health-associated Lachnospiraceae which use urea in a distinct manner that is different from that of ureolytic pathogens. |
| References: | Bioinformatics. 2024 Jul 1;40(7):. (PMID: 38991828) J Clin Invest. 1959 Sep;38:1617-26. (PMID: 13842664) Proc Natl Acad Sci U S A. 2007 May 29;104(22):9475-80. (PMID: 17517610) Mucosal Immunol. 2019 Jan;12(1):1-9. (PMID: 29988120) Acc Chem Res. 2011 Jul 19;44(7):520-30. (PMID: 21542631) Nature. 2019 May;569(7758):655-662. (PMID: 31142855) Nucleic Acids Res. 2024 Jul 5;52(W1):W521-W525. (PMID: 38597606) Infect Immun. 2012 Aug;80(8):2589-600. (PMID: 22665380) Nat Biotechnol. 2019 Feb;37(2):179-185. (PMID: 30718868) Cell Host Microbe. 2021 Mar 10;29(3):378-393.e5. (PMID: 33539766) J Bacteriol. 2003 Dec;185(23):6773-9. (PMID: 14617641) FEMS Microbiol Ecol. 2021 Mar 8;97(3):. (PMID: 33538807) Nat Biotechnol. 2021 Jan;39(1):105-114. (PMID: 32690973) Cell Host Microbe. 2018 Aug 8;24(2):296-307.e7. (PMID: 30057174) J Bacteriol. 1996 Nov;178(22):6487-95. (PMID: 8932305) Appl Environ Microbiol. 1979 Mar;37(3):379-82. (PMID: 36839) Res Microbiol. 2006 Mar;157(2):184-93. (PMID: 16139482) Microbiol Spectr. 2022 Jun 29;10(3):e0276021. (PMID: 35467410) Elife. 2021 May 04;10:. (PMID: 33944776) Genome Biol. 2009;10(3):R25. (PMID: 19261174) Gut Microbes. 2023 Jan-Dec;15(1):2192546. (PMID: 36967532) Antibiotics (Basel). 2019 Dec 13;8(4):. (PMID: 31847278) Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404) mBio. 2023 Aug 31;14(4):e0075323. (PMID: 37432034) Science. 2022 Jan 28;375(6579):460-463. (PMID: 35084962) Environ Microbiol. 2009 Aug;11(8):2112-22. (PMID: 19397676) J Crohns Colitis. 2021 Dec 18;15(12):2066-2077. (PMID: 34111242) J Exp Med. 2019 Jan 7;216(1):84-98. (PMID: 30563917) Gigascience. 2021 Feb 16;10(2):. (PMID: 33590861) Infect Immun. 2015 Sep;83(9):3418-27. (PMID: 26056382) Nature. 2024 Jun;630(8016):493-500. (PMID: 38718835) Gut Microbes. 2016 May 3;7(3):189-200. (PMID: 26963409) Cell Host Microbe. 2020 Jun 10;27(6):1001-1013.e9. (PMID: 32348781) Proc Nutr Soc. 1984 Jan;43(1):77-86. (PMID: 6709639) J Biol Chem. 2004 Mar 12;279(11):9944-50. (PMID: 14701805) Cell Host Microbe. 2020 Jul 8;28(1):134-146.e4. (PMID: 32492369) Gut. 1987 Oct;28(10):1221-7. (PMID: 3678950) Environ Microbiol. 2022 Jul;24(7):3111-3123. (PMID: 35466558) Sci Adv. 2019 Mar 20;5(3):eaav8423. (PMID: 30906870) Microbiology (Reading). 2003 Dec;149(Pt 12):3383-3394. (PMID: 14663072) Appl Environ Microbiol. 2003 Nov;69(11):6345-53. (PMID: 14602585) Nat Microbiol. 2024 Jul;9(7):1700-1712. (PMID: 38914826) Nat Med. 2019 Sep;25(9):1442-1452. (PMID: 31477907) Appl Environ Microbiol. 2014 Sep;80(17):5386-93. (PMID: 24951785) Lett Appl Microbiol. 2014 Jan;58(1):31-41. (PMID: 24001038) Cell Host Microbe. 2022 Apr 13;30(4):583-598.e8. (PMID: 35421353) Microbiol Rev. 1995 Sep;59(3):451-80. (PMID: 7565414) Bioinformatics. 2014 Jul 15;30(14):2068-9. (PMID: 24642063) |
| Contributed Indexing: | Keywords: Anaerostipes; Blautia; Lachnospiraceae; Urease; acetate; acetogenesis; acidification; butyrate; cross-feeding; microbiota; short-chain fatty acids; urea; urea-derived acetate production |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 8W8T17847W (Urea) 0 (Fatty Acids, Volatile) 7440-44-0 (Carbon) 0 (Bacterial Proteins) |
| Entry Date(s): | Date Created: 20250415 Date Completed: 20250415 Latest Revision: 20260127 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12001548 |
| DOI: | 10.1080/19490976.2025.2492376 |
| PMID: | 40231625 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1949-0984 |
|---|---|
| DOI: | 10.1080/19490976.2025.2492376 |