Academic Journal
Utilizing blood inflammatory markers in alcohol studies: Considerations and recommendations for study design, sample collection, and data analysis.
| Τίτλος: | Utilizing blood inflammatory markers in alcohol studies: Considerations and recommendations for study design, sample collection, and data analysis. |
|---|---|
| Συγγραφείς: | Grodin EN; Department of Psychiatry and Biobehavioral Sciences, University of California at Los Angeles, Los Angeles, CA, USA; Cousins Center for Psychoneuroimmunology, University of California at Los Angeles, Los Angeles, CA, USA., Karoly H; Department of Psychiatry, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, USA., Browning BD; Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, SC, USA., Coleman L; Department of Pharmacology, Bowles Center for Alcohol Studies, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC, USA., Farokhnia M; Clinical Psychoneuroendocrinology and Neuropsychopharmacology Section, Translational Addiction Medicine Branch, National Institute on Drug Abuse Intramural Research Program and National Institute on Alcohol Abuse and Alcoholism Division of Intramural Clinical and Biological Research, National Institutes of Health, Baltimore, Bethesda, MD, USA; Department of Mental Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA., Kryszak LA; National Institute on Drug Abuse, Intramural Research Program, Translational Analytical Core, National Institutes of Health, Baltimore, MD, USA., Meredith LR; Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, SC, USA., Squeglia LM; Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, SC, USA. Electronic address: squegli@musc.edu. |
| Πηγή: | Neuroscience and biobehavioral reviews [Neurosci Biobehav Rev] 2025 Jun; Vol. 173, pp. 106142. Date of Electronic Publication: 2025 Apr 09. |
| Τύπος έκδοσης: | Journal Article; Review |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Pergamon Press Country of Publication: United States NLM ID: 7806090 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7528 (Electronic) Linking ISSN: 01497634 NLM ISO Abbreviation: Neurosci Biobehav Rev Subsets: MEDLINE |
| Imprint Name(s): | Publication: New York Ny : Pergamon Press Original Publication: Fayetteville, N. Y., ANKHO International Inc. |
| Ιατρικοί όροι (MeSH): | Biomarkers*/blood , Research Design*/standards , Alcoholism*/blood , Alcoholism*/immunology , Inflammation*/blood , Inflammation*/immunology , Cytokines*/blood, Humans ; Data Analysis |
| Περίληψη: | A large body of evidence suggests that heavy alcohol use is associated with dysregulated immune function, and that immune dysfunction in turn contributes to the pathophysiology of alcohol use disorder (AUD). As such, alcohol researchers have increasingly begun to include measurements of immune function-primarily peripheral circulating cytokines-in human studies, with the goal of testing associations with clinically-relevant behavioral measures. To date, findings and implications from these studies have been inconsistent and difficult to interpret, likely due to methodological challenges related to study design and implementation. In particular, the existing literature has demonstrated sample processing concerns, differences in assay methods, limited selection of analytes, and sample selection biases, all of which may contribute to inconsistent results. We briefly review the field, discuss these and other challenges, and propose guidance for designing studies on inflammation among heavy-drinking human participants. We note that conducting such studies requires appreciable consideration and planning, and ideally should involve an interdisciplinary team of experts, including immunologists, physiologists, and technical experts in bioassays, alongside experts in the field of interest (e.g., AUD). We highlight the importance of considering participant selection, analyte selection, sample collection, sample handling and storage, and assay methods, and suggest that the field move towards standardization of procedures and reporting. We propose that undertaking these changes in study design and implementation should produce consilience in findings and aid in our overall understanding of the complex relationship between alcohol exposure and immune function. (Copyright © 2025 Elsevier Ltd. All rights reserved.) |
| References: | Alcohol Clin Exp Res (Hoboken). 2023 Jan;47(1):36-44. (PMID: 36446606) J Clin Invest. 2024 Jun 3;134(11):. (PMID: 38828729) Brain Behav Immun. 2012 Aug;26(6):911-8. (PMID: 22521198) Food Chem Toxicol. 2007 Jun;45(6):904-9. (PMID: 17210215) Braz J Psychiatry. 2022 Aug 22;44(5):548-556. (PMID: 35995431) Clin Biochem. 2019 Mar;65:38-44. (PMID: 30633878) Brain Behav Immun. 2009 Oct;23(7):887-97. (PMID: 19389469) Int Rev Immunol. 1998;16(5-6):635-49. (PMID: 9646180) Brain Behav Immun. 2021 Oct;97:349-364. (PMID: 34343618) Psychiatry Res. 1998 May 8;78(3):151-61. (PMID: 9657419) Alcohol Res. 2015;37(2):237-50. (PMID: 26695748) Cytokine. 2017 Sep;97:86-95. (PMID: 28595117) Psychoneuroendocrinology. 2021 Feb;124:105069. (PMID: 33316694) Neuropsychobiology. 1999;39(3):144-50. (PMID: 10087459) J Immunol Methods. 1997 Feb 14;201(1):99-114. (PMID: 9032413) PLoS One. 2012;7(10):e46423. (PMID: 23110049) Cell Mol Biol (Noisy-le-grand). 2001 Jun;47(4):695-702. (PMID: 11502077) Alcohol. 2004 Apr;32(3):243-7. (PMID: 15282117) Am J Gastroenterol. 2005 Jun;100(6):1303-10. (PMID: 15929761) Alcohol Clin Exp Res (Hoboken). 2023 Jul;47(7):1352-1363. (PMID: 37208927) Brain Behav Immun Health. 2021 Nov 17;18:100391. (PMID: 34877552) BMC Immunol. 2009 Sep 28;10:52. (PMID: 19785746) Proc Natl Acad Sci U S A. 2014 Oct 21;111(42):E4485-93. (PMID: 25288760) Mol Psychiatry. 2016 Dec;21(12):1696-1709. (PMID: 26903267) J Periodontol. 2015 Sep;86(9):1058-68. (PMID: 26062839) J Hepatol. 1998 May;28(5):771-7. (PMID: 9625311) Int J Mol Sci. 2016 Mar 29;17(4):472. (PMID: 27043532) J Leukoc Biol. 2016 Nov;100(5):1191-1199. (PMID: 27256567) Cytokine. 2020 Jan;125:154829. (PMID: 31472404) Drug Alcohol Depend. 2021 Aug 1;225:108771. (PMID: 34052691) J Immunol. 1998 Oct 15;161(8):4283-8. (PMID: 9780204) J Immunoassay Immunochem. 2024 Jan 2;45(1):38-49. (PMID: 37953614) Cytokine. 2000 Nov;12(11):1712-6. (PMID: 11052823) Cytokine. 2000 Sep;12(9):1437-40. (PMID: 10976010) J Psychopharmacol. 2020 Nov;34(11):1250-1260. (PMID: 32536325) Brain Behav Immun. 2013 Nov;34:164-75. (PMID: 23999491) Curr Opin Neurobiol. 2013 Aug;23(4):513-20. (PMID: 23434064) Cannabis Cannabinoid Res. 2021 Jun;6(3):177-195. (PMID: 33998900) Brain Behav Immun Health. 2023 Jun 11;31:100645. (PMID: 37484196) Nat Rev Immunol. 2002 Jan;2(1):37-45. (PMID: 11905836) Psychoneuroendocrinology. 2017 Feb;76:183-191. (PMID: 27951519) Drug Alcohol Depend. 2016 Jun 01;163:77-83. (PMID: 27068251) Alcohol Alcohol. 2012 Jul-Aug;47(4):390-6. (PMID: 22510812) PLoS One. 2016 Nov 10;11(11):e0165799. (PMID: 27832117) Biomedicines. 2022 Apr 20;10(5):. (PMID: 35625687) Pharmacol Res Perspect. 2015 Mar;3(2):e00131. (PMID: 26038706) Hepatology. 2017 Aug;66(2):575-590. (PMID: 28466561) Brain Behav Immun. 2020 Oct;89:501-512. (PMID: 32805393) J Immunol Methods. 1992 Aug 30;153(1-2):125-31. (PMID: 1517582) Cytokine. 2021 Jul;143:155506. (PMID: 33846070) Alcohol Clin Exp Res. 1997 Nov;21(8):1530-3. (PMID: 9394127) Neuropharmacology. 2017 Aug 1;122:56-73. (PMID: 28159648) Ann Allergy Asthma Immunol. 1999 Jul;83(1):61-7. (PMID: 10437818) Neurosci Biobehav Rev. 2021 Jun;125:208-220. (PMID: 33639179) Cytokine. 2021 Sep;145:155458. (PMID: 33581983) Healthcare (Basel). 2021 Apr 01;9(4):. (PMID: 33916085) Alcohol Clin Exp Res. 2011 May;35(5):782-6. (PMID: 21284666) Alcohol. 2014 Nov;48(7):671-6. (PMID: 25262503) Alcohol Clin Exp Res. 2014 May;38(5):1301-6. (PMID: 24689518) Med Oral Patol Oral Cir Bucal. 2012 Jan 01;17(1):e10-5. (PMID: 21743397) Addiction. 2020 Dec;115(12):2257-2267. (PMID: 32533781) Curr Opin Immunol. 2011 Oct;23(5):632-8. (PMID: 21852079) Cureus. 2024 May 2;16(5):e59522. (PMID: 38826899) Transl Psychiatry. 2017 Jan 10;7(1):e996. (PMID: 28072413) |
| Grant Information: | K01 AA029712 United States AA NIAAA NIH HHS; K24 AA031052 United States AA NIAAA NIH HHS |
| Contributed Indexing: | Keywords: Alcohol; Cytokines; Heavy drinking; Immune; Inflammation |
| Substance Nomenclature: | 0 (Biomarkers) 0 (Cytokines) |
| Entry Date(s): | Date Created: 20250411 Date Completed: 20250506 Latest Revision: 20260813 |
| Update Code: | 20260813 |
| PubMed Central ID: | PMC13003697 |
| DOI: | 10.1016/j.neubiorev.2025.106142 |
| PMID: | 40216171 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-7528 |
|---|---|
| DOI: | 10.1016/j.neubiorev.2025.106142 |