Fluorescent Probe for the pH-Independent Rapid and Sensitive Direct Detection of Urease-Producing Bacteria.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Fluorescent Probe for the pH-Independent Rapid and Sensitive Direct Detection of Urease-Producing Bacteria.
Συγγραφείς:	Albrich WC; Division of Infectious Diseases, Infection Prevention and Travel Medicine, Kantonsspital St. Gallen, Kantonsspital St. Gallen, Rorschacher Strasse 95, St. Gallen 9007, Switzerland., Kahlert CR; Division of Infectious Diseases, Infection Prevention and Travel Medicine, Kantonsspital St. Gallen, Kantonsspital St. Gallen, Rorschacher Strasse 95, St. Gallen 9007, Switzerland.; Infectious Diseases and Hospital Epidemiology, Children's Hospital St. Gallen, Claudiusstr. 6, St. Gallen 9006, Switzerland., Nigg S; Division of Infectious Diseases, Infection Prevention and Travel Medicine, Kantonsspital St. Gallen, Kantonsspital St. Gallen, Rorschacher Strasse 95, St. Gallen 9007, Switzerland., Boesel LF; Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomimetic Membranes and Textiles, Lerchenfeldstrasse 5, St. Gallen 9014, Switzerland., Giovannini G; Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomimetic Membranes and Textiles, Lerchenfeldstrasse 5, St. Gallen 9014, Switzerland.
Πηγή:	Analytical chemistry [Anal Chem] 2024 Dec 31; Vol. 96 (52), pp. 20578-20586. Date of Electronic Publication: 2024 Dec 16.
Τύπος έκδοσης:	Journal Article; Research Support, Non-U.S. Gov't
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE
Imprint Name(s):	Original Publication: Washington, American Chemical Society.
Ιατρικοί όροι (MeSH):	Urease/metabolism , Urease/analysis , Urease/biosynthesis , Fluorescent Dyes/chemistry , Klebsiella pneumoniae/enzymology , Klebsiella pneumoniae/isolation & purification , Enterobacter cloacae/enzymology , Enterobacter cloacae/isolation & purification, Hydrogen-Ion Concentration ; Limit of Detection ; Humans
Περίληψη:	Urease-producing bacteria are highly relevant in medicine due to their role in various pathogenic processes and their impact on human health, causing serious medical conditions such as peptic ulcer disease, gastric cancer, and respiratory and urinary tract infections. In this work, we designed fluorescent polymeric particles (PNP_FITC) to enable the detection of urease-producing bacteria by targeting the enzymatic activity of urease. In particular, the PNP_FITC matrix is degraded by urease, leading to a measurable increase in the intensity of the fluorescent signal. This approach is designed to directly sense urease activity and is therefore not affected by environmental parameters, unlike standard methods based on the quantification of enzymatic metabolites (i.e., NH3 and CO2). PNP_FITC exhibited a linear response in the urease range of 0-7.5 U/mL, with a calculated limit of detection of 0.4 U/mL. The direct detection of enzymatic activity makes PNP_FITC suitable for detecting urease-producing bacteria (Klebsiella pneumoniae and Enterobacter cloacae) with a detection limit of 10 ∧ 3 bacteria/mL, which were not detectable using the pH-based method employed as the reference in this work. Given the improvements achieved with PNP_FITC in terms of robustness, sensitivity, and selectivity of urease detection compared to the standard methods, this approach represents a step forward toward the development of advanced point-of-care, enabling the prompt diagnosis of bacterial infections.
Substance Nomenclature:	EC 3.5.1.5 (Urease) 0 (Fluorescent Dyes)
Entry Date(s):	Date Created: 20241216 Date Completed: 20250427 Latest Revision: 20260622
Update Code:	20260622
PubMed Central ID:	PMC11696831
DOI:	10.1021/acs.analchem.4c05182
PMID:	39679657
Βάση Δεδομένων:	MEDLINE

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