Visualization and Analyses of Cytoplasmic Streaming in C. elegans Zygotes.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Visualization and Analyses of Cytoplasmic Streaming in C. elegans Zygotes.
Συγγραφείς:	Kimura K; Division of Developmental Physiology, Institute for Genetic Medicine, Hokkaido University, Hokkaido, Japan. kekimura@igm.hokudai.ac.jp., Motegi F; Division of Developmental Physiology, Institute for Genetic Medicine, Hokkaido University, Hokkaido, Japan. motegi@igm.hokudai.ac.jp.
Πηγή:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2872, pp. 131-139.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
Imprint Name(s):	Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press
Ιατρικοί όροι (MeSH):	Caenorhabditis elegans/metabolism , Caenorhabditis elegans/cytology , Zygote/cytology , Zygote/metabolism , Cytoplasmic Streaming*/physiology, Microtubules/metabolism ; Oocytes/metabolism ; Oocytes/cytology ; Cytoplasm/metabolism ; Animals ; Oogenesis
Περίληψη:	Cytoplasmic streaming is the bulk flow of cytoplasm observed, not only in plants but also in animal oocytes and embryos. The flow of viscous fluid within the cytoplasm generates forces that re-arrange intracellular organelles, such as mitotic spindles and nuclei, to regulate cell growth, migration, and polarity. Cytoplasmic streaming is established by motor proteins and the viscoelastic cytoskeleton, including the actin filaments and microtubules. The mode of action can be changed in response to cell fate and developmental stage; however, its regulatory mechanism is unclear. The nematode Caenorhabditis elegans is a valuable model organism for analyzing cytoplasmic streaming because actin- and microtubule-dependent flows occur during oogenesis and embryogenesis, respectively. In this chapter, we describe methods for visualizing and quantitatively analyzing cytoplasmic streaming in C. elegans oocytes and embryos. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
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Contributed Indexing:	Keywords: Actin; C. elegans; Cytoplasmic streaming; Fluid flow; Microtubules; Oocyte; Zygote
Entry Date(s):	Date Created: 20241201 Date Completed: 20241201 Latest Revision: 20260909
Update Code:	20260909
DOI:	10.1007/978-1-0716-4224-5_9
PMID:	39616573
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	1940-6029
DOI:	10.1007/978-1-0716-4224-5_9