Academic Journal
Escherichia coli Orf135 (NudG) mutant protein specific for oxidized dATP.
| Τίτλος: | Escherichia coli Orf135 (NudG) mutant protein specific for oxidized dATP. |
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| Συγγραφείς: | Kamiya H; Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan. |
| Πηγή: | Nucleosides, nucleotides & nucleic acids [Nucleosides Nucleotides Nucleic Acids] 2025; Vol. 44 (11), pp. 886-893. Date of Electronic Publication: 2024 Oct 11. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Taylor & Francis Country of Publication: United States NLM ID: 100892832 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2335 (Electronic) Linking ISSN: 15257770 NLM ISO Abbreviation: Nucleosides Nucleotides Nucleic Acids Subsets: MEDLINE |
| Imprint Name(s): | Publication: Philadelphia : Taylor & Francis Original Publication: Monticello, NY : Marcel Dekker, c2000- |
| Ιατρικοί όροι (MeSH): | Escherichia coli*/genetics , Escherichia coli*/enzymology , Escherichia coli*/metabolism , Pyrophosphatases*/genetics , Pyrophosphatases*/metabolism , Pyrophosphatases*/chemistry , Escherichia coli Proteins*/metabolism , Escherichia coli Proteins*/genetics , Escherichia coli Proteins*/chemistry , Deoxyadenine Nucleotides*/metabolism , Deoxyadenine Nucleotides*/chemistry , Mutant Proteins*/metabolism , Mutant Proteins*/genetics , Mutant Proteins*/chemistry, Oxidation-Reduction ; Substrate Specificity ; Mutation ; Nudix Hydrolases |
| Περίληψη: | Damaged 2'-deoxyribonucleotides cause mutations, cancer, cell death, and aging. The Escherichia coli Orf135 (NudG) protein catalyzes the hydrolysis of various 2'-deoxyribonucleotides including an oxidized form of dATP, 2-oxo-1,2-dihydro-2'-deoxyadenosine 5'-triphosphate (dAOTP, 2-hydroxy-2'-deoxyadenosine 5'-triphosphate). The best substrate is 5-methyl-2'-deoxycytidine 5'-triphosphate (dCmTP), and the protein prefers dCmTP over dAOTP by ∼200-fold in vitro. To make the enzyme specific for the mutagenic nucleotide dAOTP, a double mutant protein (E33A plus D118E) was designed and produced in E. coli. The purified mutant protein showed one order of magnitude higher dAOTP preference over dCmTP. The split protein based on this mutant may potentially be used to detect dAOTP in living cells. |
| Contributed Indexing: | Keywords: 2-dihydro-2’-deoxyadenosine 5’-triphosphate; 2-oxo-1; 5-methyl-2’-deoxycytidine 5’-triphosphate; NudG; Orf135; substrate specificity |
| Substance Nomenclature: | EC 3.6.1.- (Pyrophosphatases) 0 (Escherichia coli Proteins) 0 (Deoxyadenine Nucleotides) EC 3.6.1.- (Nudix Hydrolases) K8KCC8SH6N (2'-deoxyadenosine triphosphate) 0 (Mutant Proteins) |
| Entry Date(s): | Date Created: 20241011 Date Completed: 20250925 Latest Revision: 20250925 |
| Update Code: | 20260130 |
| DOI: | 10.1080/15257770.2024.2413878 |
| PMID: | 39393082 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1532-2335 |
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| DOI: | 10.1080/15257770.2024.2413878 |