Academic Journal
Gardnerella subgroup dominant microbiomes are associated with divergent cervicovaginal immune responses in a longitudinal cohort of Kenyan women.
| Τίτλος: | Gardnerella subgroup dominant microbiomes are associated with divergent cervicovaginal immune responses in a longitudinal cohort of Kenyan women. |
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| Συγγραφείς: | Shvartsman E; Department of Medical Microbiology and Infectious Disease, University of Manitoba, Winnipeg, MB, Canada.; JC Wilt Infectious Diseases Research Centre, Winnipeg, MB, Canada.; Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada., Perciani CT; Department of Immunology, University of Toronto, Toronto, ON, Canada., Richmond MEI; Department of Medical Microbiology and Infectious Disease, University of Manitoba, Winnipeg, MB, Canada.; JC Wilt Infectious Diseases Research Centre, Winnipeg, MB, Canada., Russell JNH; JC Wilt Infectious Diseases Research Centre, Winnipeg, MB, Canada.; Department of Immunology, University of Toronto, Toronto, ON, Canada., Tough RH; Department of Medical Microbiology and Infectious Disease, University of Manitoba, Winnipeg, MB, Canada.; JC Wilt Infectious Diseases Research Centre, Winnipeg, MB, Canada., Vancuren SJ; Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada., Hill JE; Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada., Kavi-Icr; Kenyan AIDS Vaccine Initiative-Institute of Clinical Research (KAVI-ICR), University of Nairobi, Nairobi, Kenya., Jaoko W; Kenyan AIDS Vaccine Initiative-Institute of Clinical Research (KAVI-ICR), University of Nairobi, Nairobi, Kenya., McKinnon LR; Department of Medical Microbiology and Infectious Disease, University of Manitoba, Winnipeg, MB, Canada.; JC Wilt Infectious Diseases Research Centre, Winnipeg, MB, Canada.; Centre for the AIDS Program of Research in South Africa (CAPRISA), Durban, South Africa., Sandstrom PA; Department of Medical Microbiology and Infectious Disease, University of Manitoba, Winnipeg, MB, Canada.; JC Wilt Infectious Diseases Research Centre, Winnipeg, MB, Canada., MacDonald KS; Department of Medical Microbiology and Infectious Disease, University of Manitoba, Winnipeg, MB, Canada.; JC Wilt Infectious Diseases Research Centre, Winnipeg, MB, Canada.; Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada.; Department of Immunology, University of Toronto, Toronto, ON, Canada. |
| Πηγή: | Frontiers in immunology [Front Immunol] 2023 Jan 16; Vol. 13, pp. 974195. Date of Electronic Publication: 2023 Jan 16 (Print Publication: 2022). |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: [Lausanne : Frontiers Research Foundation] |
| Ιατρικοί όροι (MeSH): | Gardnerella* , Microbiota* , Vaginosis, Bacterial*, Kenya/epidemiology ; Lactobacillus/genetics ; Vagina/immunology ; Vagina/microbiology ; Female ; Humans ; Chemokine CXCL10 ; HIV Infections ; Immunity |
| Περίληψη: | Most cervicovaginal microbiome-immunology studies to date have relied on 16S rDNA microbial profiling which does not resolve the molecular subgroups of Gardnerella, believed to be central to the pathogenesis of bacterial vaginosis (BV) and subsequent risk of HIV acquisition. Here we used the cpn60 universal target which in addition to other microbial taxa, resolves four Gardnerella subgroups, for cervicovaginal microbial profiling in a longitudinal cohort of Kenyan women to examine associations with cellular and soluble markers of inflammation and HIV susceptibility. Participants (N = 41) were sampled, contributing 362 samples for microbiome analysis. All non-Lactobacillus dominant microbial communities were associated with high pro-inflammatory cytokine levels. Divergent associations were observed among different Gardnerella subgroup dominated communities with respect to the chemokine IP-10. Specifically, Gardnerella subgroup A dominant and polymicrobial communities were associated with reduced concentrations of IP-10 in adjusted linear mixed models (p<0.0001), compared to microbial communities dominated by Lactobacillus (non-iners) species. However, these associations did not translate to significant differences in the proportion or absolute number of CCR5, HLA-DR and CD38 expressed on cervical CD4+ T- cells. These findings suggest that some associations between Gardnerella subgroup dominant microbiomes and mucosal immunity differ and are relevant for the study of BV-pathogenesis and understanding the mechanisms of BV-associated HIV risk. (Copyright © 2023 Shvartsman, Perciani, Richmond, Russell, Tough, Vancuren, Hill, KAVI-ICR, Jaoko, McKinnon, Sandstrom and MacDonald.) |
| Competing Interests: | The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. |
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| Grant Information: | THA-11960 Canada CIHR |
| Contributed Indexing: | Keywords: HIV susceptibility; IP-10 (CXCL-10); bacterial vaginosis (BV); cpn60; cytokines; gardnerella species; mucosal immunity; vaginal microbiome |
| Substance Nomenclature: | 0 (Chemokine CXCL10) |
| Entry Date(s): | Date Created: 20230202 Date Completed: 20230206 Latest Revision: 20230217 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC9886495 |
| DOI: | 10.3389/fimmu.2022.974195 |
| PMID: | 36726972 |
| Βάση Δεδομένων: | MEDLINE |
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