Academic Journal
Rapid fractionation of mitochondria from mouse liver and heart reveals in vivo metabolite compartmentation.
| Τίτλος: | Rapid fractionation of mitochondria from mouse liver and heart reveals in vivo metabolite compartmentation. |
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| Συγγραφείς: | Allen FM; MRC Mitochondrial Biology Unit, University of Cambridge, UK., Costa ASH; MRC Cancer Unit, University of Cambridge, UK., Gruszczyk AV; MRC Mitochondrial Biology Unit, University of Cambridge, UK., Bates GR; MRC Mitochondrial Biology Unit, University of Cambridge, UK., Prag HA; MRC Mitochondrial Biology Unit, University of Cambridge, UK., Nikitopoulou E; MRC Cancer Unit, University of Cambridge, UK., Viscomi C; Department of Biomedical Sciences, University of Padova, Italy., Frezza C; MRC Cancer Unit, University of Cambridge, UK., James AM; MRC Mitochondrial Biology Unit, University of Cambridge, UK., Murphy MP; MRC Mitochondrial Biology Unit, University of Cambridge, UK.; Department of Medicine, University of Cambridge, UK. |
| Πηγή: | FEBS letters [FEBS Lett] 2023 Jan; Vol. 597 (2), pp. 246-261. Date of Electronic Publication: 2022 Oct 27. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 0155157 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3468 (Electronic) Linking ISSN: 00145793 NLM ISO Abbreviation: FEBS Lett Subsets: MEDLINE |
| Imprint Name(s): | Publication: Jan. 2016- : West Sussex : John Wiley & Sons Ltd. Original Publication: Amsterdam, North-Holland on behalf of the Federation of European Biochemical Societies. |
| Ιατρικοί όροι (MeSH): | Mitochondria* , Heart*, Cytosol/metabolism ; Liver/metabolism ; Mitochondria, Liver/metabolism ; Mitochondria, Heart/metabolism ; Cell Fractionation/methods ; Mice ; Animals |
| Περίληψη: | The compartmentation and distribution of metabolites between mitochondria and the rest of the cell is a key parameter of cell signalling and pathology. Here, we have developed a rapid fractionation procedure that enables us to take mouse heart and liver from in vivo and within ~ 30 s stabilise the distribution of metabolites between mitochondria and the cytosol by rapid cooling, homogenisation and dilution. This is followed by centrifugation of mitochondria through an oil layer to separate mitochondrial and cytosolic fractions for subsequent metabolic analysis. Using this procedure revealed the in vivo compartmentation of mitochondrial metabolites and will enable the assessment of the distribution of metabolites between the cytosol and mitochondria during a range of situations in vivo. (© 2022 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.) |
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| Grant Information: | 220257 United Kingdom WT_ Wellcome Trust; MC_UU_12022/6 United Kingdom MRC_ Medical Research Council; MC_UU_12022/7 United Kingdom MRC_ Medical Research Council; 220257/Z/20/Z United Kingdom WT_ Wellcome Trust |
| Contributed Indexing: | Keywords: in vivo; compartmentation; ischemia; metabolites; mitochondria; rapid fractionation |
| Entry Date(s): | Date Created: 20221011 Date Completed: 20230125 Latest Revision: 20250530 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC7614208 |
| DOI: | 10.1002/1873-3468.14511 |
| PMID: | 36217875 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-3468 |
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| DOI: | 10.1002/1873-3468.14511 |