Academic Journal
Best practice for improved accuracy: A critical reassessment of van't Hoff analysis of melt curves.
| Τίτλος: | Best practice for improved accuracy: A critical reassessment of van't Hoff analysis of melt curves. |
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| Συγγραφείς: | Majikes JM; Microsystem and Nanotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland. Electronic address: jacob.majikes@nist.gov., Zwolak M; Microsystem and Nanotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland., Liddle JA; Microsystem and Nanotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland. Electronic address: liddle@nist.gov. |
| Πηγή: | Biophysical journal [Biophys J] 2022 Jun 07; Vol. 121 (11), pp. 1986-2001. Date of Electronic Publication: 2022 May 10. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Cell Press Country of Publication: United States NLM ID: 0370626 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1542-0086 (Electronic) Linking ISSN: 00063495 NLM ISO Abbreviation: Biophys J Subsets: MEDLINE |
| Imprint Name(s): | Publication: Cambridge, MA : Cell Press Original Publication: New York, Published by Rockefeller University Press [etc.] for the Biophysical Society. |
| Ιατρικοί όροι (MeSH): | Thermodynamics* |
| Περίληψη: | Biomolecular thermodynamics, particularly for DNA, are frequently determined via van't Hoff analysis of optically measured melt curves. Accurate and precise values of thermodynamic parameters are essential for the modeling of complex systems involving cooperative effects, such as RNA tertiary structure and DNA origami, because the uncertainties associated with each motif in a folding energy landscape can compound, significantly reducing the power of predictive models. We follow the sources of uncertainty as they propagate through a typical van't Hoff analysis to derive best practices for melt experiments and subsequent data analysis, assuming perfect signal baseline correction. With appropriately designed experiments and analysis, a van't Hoff approach can provide surprisingly high precision, e.g., enthalpies may be determined with a precision as low as 10-2 kJ ⋅ mol-1 for an 8-base DNA oligomer. (Published by Elsevier Inc.) |
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| Entry Date(s): | Date Created: 20220513 Date Completed: 20220610 Latest Revision: 20230612 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC9247556 |
| DOI: | 10.1016/j.bpj.2022.05.008 |
| PMID: | 35546781 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1542-0086 |
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| DOI: | 10.1016/j.bpj.2022.05.008 |