Academic Journal
Temperature-dependency urease activity in Vibrio parahaemolyticus is related to transcriptional activator UreR.
| Τίτλος: | Temperature-dependency urease activity in Vibrio parahaemolyticus is related to transcriptional activator UreR. |
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| Συγγραφείς: | Park KS; Department of Food Science and Biotechnology, College of Ocean Science and Technology, Kunsan Nation al University, Kunsan 573-701, Korea., Lee SJ, Chung YH, Iida T, Honda T |
| Πηγή: | Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2009 Nov; Vol. 19 (11), pp. 1456-63. |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Korean Society for Microbiology and Biotechnology Country of Publication: Korea (South) NLM ID: 9431852 Publication Model: Print Cited Medium: Print ISSN: 1017-7825 (Print) Linking ISSN: 10177825 NLM ISO Abbreviation: J Microbiol Biotechnol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2002- : Seoul : Korean Society for Microbiology and Biotechnology Original Publication: Seoul : Korean Society for Applied Microbiology, 1991-2001. |
| Ιατρικοί όροι (MeSH): | Bacterial Proteins*/genetics , Bacterial Proteins*/metabolism , Trans-Activators*/genetics , Trans-Activators*/metabolism , Vibrio parahaemolyticus*/genetics , Vibrio parahaemolyticus*/metabolism , Temperature*, Urease/*biosynthesis, DNA, Bacterial/analysis ; DNA, Bacterial/genetics ; Urease/genetics ; beta-Galactosidase/metabolism ; Base Sequence ; Cloning, Molecular ; Gene Deletion ; Gene Expression Regulation, Bacterial ; Molecular Sequence Data ; Promoter Regions, Genetic ; Sequence Analysis, DNA ; Species Specificity |
| Περίληψη: | Vibrio parahaemolyticus possessing urease-positive property is relatively rare, but such strains consistently exhibit the TDH-related hemolysin (TRH) gene. In this study, we examined the effects of incubation temperature on urease activity expression, using the TH3996 and AQ4673 strains where the enzyme activity is known to be temperature-dependent and -independent, respectively. In the TH3996 strain, beta-galactosidase activity was 4.4-fold lower after 30 degrees C cultivation than after 37 degrees in a ureR-lacZ fusion strain, but temperature dependency was not found in ureD- or nikA-lacZ fusion strains. However, ureR-, ureD-, and nikA-lacZ fusions of the AQ4673 strain was not influenced by incubation temperature. We compared the promoter sequences of ureR between the above two strains. Intriguingly, we detected mismatches of two nucleotides between the two strains located at positions -66 and -108 upstream of the methionine initiation codon for UreR. Additionally, urease activity was not affected by culture temperature at either 30 degrees or 37 degrees by allelic introduction of the AQ4673 ureR gene into the TH3996 ureR deletion mutant. Taken together, our study demonstrates that the transcriptional factor UreR is involved in the temperature dependency of urease activity, and two nucleotides within the ureR promoter region are of particular importance for the urease activity dependency of V. parahaemolyticus. |
| Substance Nomenclature: | 0 (Bacterial Proteins) 0 (DNA, Bacterial) 0 (Trans-Activators) 0 (UreR protein, bacteria) EC 3.2.1.23 (beta-Galactosidase) EC 3.5.1.5 (Urease) |
| Entry Date(s): | Date Created: 20091210 Date Completed: 20100616 Latest Revision: 20190923 |
| Update Code: | 20260130 |
| DOI: | 10.4014/jmb.0902.0100 |
| PMID: | 19996701 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1017-7825 |
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| DOI: | 10.4014/jmb.0902.0100 |