Academic Journal
Are anti-Helicobacter pylori urease antibodies involved in atherosclerotic diseases?
| Τίτλος: | Are anti-Helicobacter pylori urease antibodies involved in atherosclerotic diseases? |
|---|---|
| Συγγραφείς: | Arabski M; Department of Microbiology, Institute of Biology, Jan Kochanowski University, ul Swietokrzyska 15, 25-406 Kielce, Poland. arabski@ujk.kielce.pl, Konieczna I, Sołowiej D, Rogoń A, Kolesińska B, Kamiński Z, Kaca W |
| Πηγή: | Clinical biochemistry [Clin Biochem] 2010 Jan; Vol. 43 (1-2), pp. 115-23. Date of Electronic Publication: 2009 Sep 26. |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 0133660 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2933 (Electronic) Linking ISSN: 00099120 NLM ISO Abbreviation: Clin Biochem Subsets: MEDLINE |
| Imprint Name(s): | Publication: Tarrytown, NY : Elsevier Science Original Publication: [Toronto] Canadian Society of Clinical Chemists. |
| Ιατρικοί όροι (MeSH): | Atherosclerosis*/enzymology , Atherosclerosis*/immunology, Antibodies, Bacterial/*immunology , Bacterial Proteins/*immunology , Helicobacter pylori/*enzymology , Urease/*immunology, Bacterial Proteins/genetics ; Epitopes/immunology ; Helicobacter Infections/enzymology ; Helicobacter Infections/immunology ; Peptides/chemistry ; Peptides/genetics ; Peptides/immunology ; Urease/genetics ; Adult ; Aged ; Aged, 80 and over ; Amino Acid Sequence ; Antibody Specificity ; Female ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; Molecular Structure ; Young Adult |
| Περίληψη: | Objectives: The ureB subunit of urease is a major target recognized by the antibodies of Helicobacter pylori-infected patients. The minimal epitope was determined to be an 8-mer peptide (H-SIKEDVQF-OH). Design and Methods: The aim of this study was to discover whether this synthetic 8-mer peptide (BK-61A) directly recognizes the anti-ureB subunit antibodies of H. pylori-infected and atherosclerotic patients. To achieve a better presentation of the epitopes to antibodies, a new isocyanuric linker was designed and used for to immobilize the peptides on a cellulose support. Results: In this study a new peptide synthesis method is presented. Anti-ureB antibodies were evaluated by the dot blot technique in 26 H. pylori-infected donors and the sera of 20 H. pylori-infected patients with atherosclerosis using the 8-mer peptide. Conclusions: The results reveal that the BK-61A peptide could be used for diagnosing the presence of anti-ureB antibodies that may be involved in the initiation of atherosclerosis. (Copyright 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.) |
| Substance Nomenclature: | 0 (Antibodies, Bacterial) 0 (Bacterial Proteins) 0 (Epitopes) 0 (Peptides) EC 3.5.1.5 (Urease) |
| Entry Date(s): | Date Created: 20090930 Date Completed: 20100330 Latest Revision: 20100127 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.clinbiochem.2009.09.016 |
| PMID: | 19786006 |
| Βάση Δεδομένων: | MEDLINE |
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