Academic Journal
The expression of an R3 lipopolysaccharide-core by pathotypes of Escherichia coli.
| Τίτλος: | The expression of an R3 lipopolysaccharide-core by pathotypes of Escherichia coli. |
|---|---|
| Συγγραφείς: | Chart H; Laboratory of Enteric Pathogens, Division of Gastrointestinal Infections, Central Public Health Laboratory, London, UK. henrik.chart@hpa.org.uk, Perry NT, Jenkins C |
| Πηγή: | Journal of applied microbiology [J Appl Microbiol] 2004; Vol. 96 (5), pp. 982-6. |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print Cited Medium: Print ISSN: 1364-5072 (Print) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2022- : Oxford : Oxford University Press Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997- |
| Ιατρικοί όροι (MeSH): | Escherichia coli/*genetics , Lipopolysaccharides/*analysis, Escherichia coli O157/genetics ; Genes, Bacterial/genetics ; Nucleic Acid Amplification Techniques/methods ; Shiga Toxins/biosynthesis ; Animals ; Cattle ; Humans ; Polymerase Chain Reaction ; Serotyping |
| Περίληψη: | Aims: To investigate the incidence of an R3 lipopolysaccharide (LPS)-core amplicon in a range of pathotypes of Escherichia coli, including Verocytotoxin-producing E. coli (VTEC), enteroaggregative E. coli (EAggEC) and enteropathogenic E. coli (EPEC). Methods and Results: A total of 100 strains of E. coli belonging to a range of pathotypes, including 41 strains of VTEC, were screened for the genes encoding the R3 LPS-core using PCR. Fifty-four per cent produced an amplicon with the R3 primer set. Of the 41 VTEC, 66% had an R3 LPS-core with a PCR product being observed with all strains belonging to serotypes O26:H11, O111ac:H- and O145:H25. However, 46% of enteroaggregative E. coli and 50% of enteropathogenic E. coli were also shown to have an R3 LPS-core structure. Conclusions: Strains with an R3 LPS-core are widely distributed within the species E. coli. Significance and Impact of the Study: Strains of E. coli with an R3 LPS-core structure appear not to be associated with a specific pathotype. |
| Substance Nomenclature: | 0 (Lipopolysaccharides) 0 (Shiga Toxins) |
| Entry Date(s): | Date Created: 20040414 Date Completed: 20040730 Latest Revision: 20061115 |
| Update Code: | 20260130 |
| DOI: | 10.1111/j.1365-2672.2004.02233.x |
| PMID: | 15078514 |
| Βάση Δεδομένων: | MEDLINE |
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