Academic Journal
A dip-and-read test strip for the determination of mercury(II) ion in aqueous samples based on urease activity inhibition.
| Title: | A dip-and-read test strip for the determination of mercury(II) ion in aqueous samples based on urease activity inhibition. |
|---|---|
| Authors: | Shi GQ; Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P 0. Box 2871, Beijing 100085, China., Jiang G |
| Source: | Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2002 Nov; Vol. 18 (11), pp. 1215-9. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Springer Country of Publication: Switzerland NLM ID: 8511078 Publication Model: Print Cited Medium: Print ISSN: 0910-6340 (Print) Linking ISSN: 09106340 NLM ISO Abbreviation: Anal Sci Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2022- : [Cham] : Springer Original Publication: Tokyo : The Society, [1985- |
| MeSH Terms: | Reagent Strips*, Mercury/*analysis , Urease/*antagonists & inhibitors , Water/*chemistry , Water Pollutants/*analysis, Biosensing Techniques/instrumentation ; Biosensing Techniques/methods ; Enzymes, Immobilized/antagonists & inhibitors ; Enzymes, Immobilized/metabolism ; Ions/analysis ; Metals, Heavy/analysis ; Urea/metabolism ; Urease/metabolism ; Calibration ; Hydrogen-Ion Concentration ; Reference Standards ; Sensitivity and Specificity ; Time Factors |
| Abstract: | A sensitive dip-and-read test strip for the determination of mercury in aqueous samples based on the inhibition of urease reaction by the ion has been developed. The strip has a circular sensing zone that containing two layers: the top layer is a cellulose acetate membrane where urease is immobilized on it; the bottom layer is a pH indicator wafer that is impregnated with urea. The principle of the measurement is based on the disappearance of a yellow spot on the pH indicator wafer. The elapsing time until the disappearance of the spot which depends on the concentration of mercury(II) ion is measured with a stopwatch. Under the experimental conditions, as low as 0.2 ng/ml mercury can be observed with the detection range from 0.2 to 200 ng/ml in water. Organomercury compounds give essentially the same response as inorganic mercury. Heavy-metal ions such as Ag(I), Cu(II), Cd(II), Ni(II), Zn(II), and Pb(II) as well as other sample matrixes basically do not interfere with the mercury measurement. |
| Substance Nomenclature: | 0 (Enzymes, Immobilized) 0 (Ions) 0 (Metals, Heavy) 0 (Reagent Strips) 0 (Water Pollutants) 059QF0KO0R (Water) 8W8T17847W (Urea) EC 3.5.1.5 (Urease) FXS1BY2PGL (Mercury) |
| Entry Date(s): | Date Created: 20021203 Date Completed: 20030220 Latest Revision: 20190826 |
| Update Code: | 20260130 |
| DOI: | 10.2116/analsci.18.1215 |
| PMID: | 12458706 |
| Database: | MEDLINE |
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