Academic Journal

不同栽培模式下巫山淫羊藿根际微生物群落结构多样性变化与有效成分 积累相关性分析.

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Τίτλος: 不同栽培模式下巫山淫羊藿根际微生物群落结构多样性变化与有效成分 积累相关性分析.
Alternate Title: Analysis of the Correlation Between Changes in Rhizosphere Microbial Community Structure Diversity and Accumulation of Active Ingredients in Epimedium wushanense Under Different Cultivation Patterns.
Συγγραφείς: 贺元川1, 卫秋阳1, 李晓明1, 罗昌树1, 刘 翔1, 陈仕江1
Πηγή: Journal of Anhui Agricultural Sciences. 9/15/2026, Vol. 54 Issue 17, p164-170. 7p.
Θεματικοί όροι: *Rhizosphere microbiology, *Cropping systems, *Microbial diversity, *Statistical correlation, *Plant species, *Metabolites, *Flavonoids, *Metagenomics
Abstract (English): [Objective]To explore the correlation between rhizosphere microbial community structure diversity and the accumulation of active components in Epimedium wushanense under different cultivation patterns. [Method]Rhizosphere soils from three-year-old E. wushanense under understory planting (LG) and corn intercropping (TG) were analyzed using high-throughput sequencing. Active components including epimedin A, B, C, icariin, and total flavonoids were measured by HPLC and UV spectrophotometry. Pearson correlation analysis was conducted to assess the relationship between microbial communities and active components. [Result]Dominant bacterial phyla included Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, and Bacteroidetes. Dominant fungal phyla were Basidiomycota, Ascomycota, and Mortierellomucota. UPGMA clustering and NMDS analysis showed similar bacterial community structures between TG and LG, but fungal communities differed significantly. Correlation analysis indicated significant negative correlations between Acidobacteria, Rokubacteria, Rozellomycota and epimedin A, B, and C, and total flavonoids, while Actinobacteria showed significant positive correlations with epimedin A, B, and C. Chloroflexi was significant negatively correlated with total flavonoids, whereas Kickxellomycota showed a significant positive correlation. [Conclusion]Cultivation patterns affect rhizosphere microbial community structure and diversity, influencing the accumulation of active ingredients and optimal harvesting time in E. wushanense. This study provides insights for rhizosphere microbial resource utilization and cultivation optimization. [ABSTRACT FROM AUTHOR]
Abstract (Chinese): [目的] 探讨不同栽培模式下巫山淫羊藿根际土壤微生物群落结构多样性及其与主要药用有效成分累积的相关性。 [方法] 以 3 年生林下种植 (LG) 和玉米套作 (TG) 的巫山淫羊藿根际土壤为研究对象, 利用高通量测序技术分析根际微生物群落结构, 并采用高效液 相色谱法和紫外吸收法测定不同时期药材的朝霍定 A 、朝霍定 B 、朝霍定 C 、淫羊藿苷、总黄酮含量。 通过皮尔逊相关性分析探讨根际土 壤微生物与有效成分积累之间的关系。 [结果] 根际细菌优势菌门为变形菌门、酸杆菌门、放线菌门、绿萼菌门和拟杆菌门, 根际真菌以 担子菌门、子囊菌门和被孢霉门为主。 UPGMA 聚类与非度量多维标定分析表明, 不同栽培模式的细菌群落结构较为相似, 真菌群落结 构差异显著。 相关性分析显示, 细菌中的酸杆菌门、棒状杆菌门和真菌中的隐真菌门与朝霍定 A 、朝霍定 B 、朝霍定 C 、总黄酮呈显著负 相关, 而细菌中的放线菌门与朝霍定 A 、朝霍定 B 、朝霍定 C 呈显著正相关。 细菌绿弯菌门与总黄酮呈显著负相关, 真菌梳霉门与其呈显 著正相关。 [结论] 不同栽培模式影响根际微生物群落结构与多样性, 进而改变巫山淫羊藿有效成分的积累动态, 导致最佳采收时期存 在差异。 该研究为巫山淫羊藿根际微生物资源开发及其高效栽培与适时采收提供理论依据。 [ABSTRACT FROM AUTHOR]
Βάση Δεδομένων: Academic Search Index
Περιγραφή
ISSN:05176611
DOI:10.3969/j.issn.0517-6611.2026.17.031