Comprehensive assessment of hyperoside: cytotoxic, anti-cancer, genoprotective, radical scavenging and computational toxicity properties through in vitro and in silico approaches.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Comprehensive assessment of hyperoside: cytotoxic, anti-cancer, genoprotective, radical scavenging and computational toxicity properties through in vitro and in silico approaches.
Συγγραφείς:	Burgazli, Aysen Yagmur¹ (AUTHOR), Avuloglu Yilmaz, Ece² (AUTHOR), Mamur, Sevcan³ (AUTHOR), Okus, Fatma⁴ (AUTHOR), Tuncay, Busenaz¹ (AUTHOR), Yuzbasioglu, Deniz⁵ (AUTHOR) deniz@gazi.edu.tr, Unal, Fatma⁵ (AUTHOR)
Πηγή:	Toxicology Mechanisms & Methods. Jun2026, p1-20. 20p. 15 Illustrations.
Θεματικοί όροι:	Antineoplastic agents, Flavonoid glycosides, Antioxidants, Cytotoxins, Toxicity testing, DNA damage, Free radical scavengers, Molecular docking
Περίληψη:	Abstract\nHIGHLIGHTSHyperoside (HYP), a plant-derived flavonoid, was evaluated for its cytotoxic, anti-cancer, genotoxic, antigenotoxic, and antioxidant properties, and potential mechanisms of action using an integrated in vitro and in silico approach. Cytotoxicity and anti-cancer activities were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in CCD18-Co healthy colon epithelial and DLD-1 colon cancer cells. At the same time, genotoxicity and antigenotoxicity potentials were examined in human lymphocytes using COMET assay. Antioxidant activity was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, and molecular docking was performed with key regulatory proteins (p53, ATM, 7O7B, and Keap1), and in silico toxicity profiling was conducted using the Percepta platform. HYP selectively reduced the viability of colon cancer cells (43.43%–70.24%) without inducing cytotoxicity in healthy colon epithelial cells (cell viability percentage ranging from 82.25% to 111.88%) after 24 and 48 h of exposure. HYP did not significantly increase DNA damage at 7.81–62.5 μg/mL; however, it exhibited antigenotoxic effects at all concentrations, significantly reducing H2O2-induced DNA damage. HYP also showed significant antioxidant activity, with DPPH inhibition ranging from 53.09% to 78.64%. Docking analyses revealed strong binding affinities for ATM and Keap1, supporting its potential role in modulating oxidative stress and DNA damage response pathways. Percepta-based toxicity profiling predicted low to moderate acute systemic toxicity, limited oral bioavailability, and no major cardiotoxic or CYP-mediated safety liabilities; notably, predicted mutagenicity alerts were not corroborated by in vitro DNA damage assessments. Overall, these findings suggest that HYP exhibits selective anticancer and antigenotoxic properties, potentially mediated by its antioxidant activity.Hyperoside, a potential genoprotective agent, reduces H2O2-induced DNA damage.Hyperoside exhibits selective cytotoxicity against DLD-1colon cancer cells.Hyperoside is an antioxidant revealing potential radical scavenging activity.Docking analysis showed strong binding of Hyperoside to ATM and Keap1 proteins.In silico toxicity profiling supported a favorable safety profile for Hyperoside.Hyperoside, a potential genoprotective agent, reduces H2O2-induced DNA damage.Hyperoside exhibits selective cytotoxicity against DLD-1colon cancer cells.Hyperoside is an antioxidant revealing potential radical scavenging activity.Docking analysis showed strong binding of Hyperoside to ATM and Keap1 proteins.In silico toxicity profiling supported a favorable safety profile for Hyperoside. [ABSTRACT FROM AUTHOR]
Βάση Δεδομένων:	Academic Search Index

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Header	DbId: asx DbLabel: Academic Search Index An: 194659521 RelevancyScore: 1452 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 1452.41931152344
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Items	– Name: Title Label: Title Group: Ti Data: Comprehensive assessment of hyperoside: cytotoxic, anti-cancer, genoprotective, radical scavenging and computational toxicity properties through <italic>in vitro</italic> and <italic>in silico</italic> approaches. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Burgazli%2C+Aysen+Yagmur%22">Burgazli, Aysen Yagmur</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Avuloglu+Yilmaz%2C+Ece%22">Avuloglu Yilmaz, Ece</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mamur%2C+Sevcan%22">Mamur, Sevcan</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Okus%2C+Fatma%22">Okus, Fatma</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tuncay%2C+Busenaz%22">Tuncay, Busenaz</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yuzbasioglu%2C+Deniz%22">Yuzbasioglu, Deniz</searchLink><relatesTo>5</relatesTo> (AUTHOR)<i> deniz@gazi.edu.tr</i><br /><searchLink fieldCode="AR" term="%22Unal%2C+Fatma%22">Unal, Fatma</searchLink><relatesTo>5</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Toxicology+Mechanisms+%26+Methods%22">Toxicology Mechanisms & Methods</searchLink>. Jun2026, p1-20. 20p. 15 Illustrations. – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Antineoplastic+agents%22">Antineoplastic agents</searchLink><br /><searchLink fieldCode="DE" term="%22Flavonoid+glycosides%22">Flavonoid glycosides</searchLink><br /><searchLink fieldCode="DE" term="%22Antioxidants%22">Antioxidants</searchLink><br /><searchLink fieldCode="DE" term="%22Cytotoxins%22">Cytotoxins</searchLink><br /><searchLink fieldCode="DE" term="%22Toxicity+testing%22">Toxicity testing</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+damage%22">DNA damage</searchLink><br /><searchLink fieldCode="DE" term="%22Free+radical+scavengers%22">Free radical scavengers</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+docking%22">Molecular docking</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abstract\nHIGHLIGHTSHyperoside (HYP), a plant-derived flavonoid, was evaluated for its cytotoxic, anti-cancer, genotoxic, antigenotoxic, and antioxidant properties, and potential mechanisms of action using an integrated <italic>in vitro</italic> and <italic>in silico</italic> approach. Cytotoxicity and anti-cancer activities were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in CCD18-Co healthy colon epithelial and DLD-1 colon cancer cells. At the same time, genotoxicity and antigenotoxicity potentials were examined in human lymphocytes using COMET assay. Antioxidant activity was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, and molecular docking was performed with key regulatory proteins (p53, ATM, 7O7B, and Keap1), and <italic>in silico</italic> toxicity profiling was conducted using the Percepta platform. HYP selectively reduced the viability of colon cancer cells (43.43%–70.24%) without inducing cytotoxicity in healthy colon epithelial cells (cell viability percentage ranging from 82.25% to 111.88%) after 24 and 48 h of exposure. HYP did not significantly increase DNA damage at 7.81–62.5 μg/mL; however, it exhibited antigenotoxic effects at all concentrations, significantly reducing H2O2-induced DNA damage. HYP also showed significant antioxidant activity, with DPPH inhibition ranging from 53.09% to 78.64%. Docking analyses revealed strong binding affinities for ATM and Keap1, supporting its potential role in modulating oxidative stress and DNA damage response pathways. Percepta-based toxicity profiling predicted low to moderate acute systemic toxicity, limited oral bioavailability, and no major cardiotoxic or CYP-mediated safety liabilities; notably, predicted mutagenicity alerts were not corroborated by <italic>in vitro</italic> DNA damage assessments. Overall, these findings suggest that HYP exhibits selective anticancer and antigenotoxic properties, potentially mediated by its antioxidant activity.Hyperoside, a potential genoprotective agent, reduces H2O2-induced DNA damage.Hyperoside exhibits selective cytotoxicity against DLD-1colon cancer cells.Hyperoside is an antioxidant revealing potential radical scavenging activity.Docking analysis showed strong binding of Hyperoside to ATM and Keap1 proteins.<italic>In silico</italic> toxicity profiling supported a favorable safety profile for Hyperoside.Hyperoside, a potential genoprotective agent, reduces H2O2-induced DNA damage.Hyperoside exhibits selective cytotoxicity against DLD-1colon cancer cells.Hyperoside is an antioxidant revealing potential radical scavenging activity.Docking analysis showed strong binding of Hyperoside to ATM and Keap1 proteins.<italic>In silico</italic> toxicity profiling supported a favorable safety profile for Hyperoside. [ABSTRACT FROM AUTHOR]
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RecordInfo	BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1080/15376516.2026.2691542 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 20 StartPage: 1 Subjects: – SubjectFull: Antineoplastic agents Type: general – SubjectFull: Flavonoid glycosides Type: general – SubjectFull: Antioxidants Type: general – SubjectFull: Cytotoxins Type: general – SubjectFull: Toxicity testing Type: general – SubjectFull: DNA damage Type: general – SubjectFull: Free radical scavengers Type: general – SubjectFull: Molecular docking Type: general Titles: – TitleFull: Comprehensive assessment of hyperoside: cytotoxic, anti-cancer, genoprotective, radical scavenging and computational toxicity properties through <italic>in vitro</italic> and <italic>in silico</italic> approaches. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Burgazli, Aysen Yagmur – PersonEntity: Name: NameFull: Avuloglu Yilmaz, Ece – PersonEntity: Name: NameFull: Mamur, Sevcan – PersonEntity: Name: NameFull: Okus, Fatma – PersonEntity: Name: NameFull: Tuncay, Busenaz – PersonEntity: Name: NameFull: Yuzbasioglu, Deniz – PersonEntity: Name: NameFull: Unal, Fatma IsPartOfRelationships: – BibEntity: Dates: – D: 17 M: 06 Text: Jun2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 15376516 Titles: – TitleFull: Toxicology Mechanisms & Methods Type: main
ResultId	1