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Exploring metaboloepigenetic variability in bovine fibroblast lines.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Exploring metaboloepigenetic variability in bovine fibroblast lines.
Συγγραφείς: Hawrylyshyn, Eric1,2 (AUTHOR), Ferraz, Marcia de A.M.M.2,3 (AUTHOR), Chan, Biankha1,2 (AUTHOR), Mastromonaco, Gabriela F.1,2 (AUTHOR) gmastromonaco@torontozoo.ca
Πηγή: Biochemistry & Cell Biology. 5/25/2026, Vol. 104, p1-14. 14p.
Θεματικοί όροι: *Fibroblasts, *Epigenetics, *Chromatin, *Somatic cell nuclear transfer, *Reproductive technology, *DNA methylation, *Bioenergetics
Περίληψη: Somatic cell-based reproductive technologies (SCRTs) offer a promising approach for preserving the genetic diversity of threatened species. SCRTs require the nuclear reprogramming of a donor somatic cell, which has low success rates (1%–5%) and can vary significantly between individuals and cell lines derived from the same individual. Somatic and pluripotent cells differ in several key characteristics, and donor cells presenting more pluripotent-like traits have been found to result in improved reprogramming outcomes. Here, we characterized 18 standardized fibroblast cell lines from six Angus bulls for several of these characteristics at the inter- and intra-individual levels: bioenergetic status, targeted metabolite abundance, global DNA methylation levels, and presence of key histone modifications. Differences in the metaboloepigenetic profiles of these cell lines were observed at both inter- and intra-individual levels. By integrating these data, we positioned cell lines along a somatic-to-pluripotent-like continuum and identified a reduced set of features that captured the dominant separation between profiles. Although functional reprogramming outcomes were not assessed here, these results provide a data-driven framework to prioritise donor cell lines for prospective induced pluripotent stem cell/somatic cell nuclear transfer validation and to guide the development of practical biomarker panels for SCRT workflows. [ABSTRACT FROM AUTHOR]
Βάση Δεδομένων: Academic Search Index
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  Data: Exploring metaboloepigenetic variability in bovine fibroblast lines.
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  Data: <searchLink fieldCode="AR" term="%22Hawrylyshyn%2C+Eric%22">Hawrylyshyn, Eric</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ferraz%2C+Marcia+de+A%2EM%2EM%2E%22">Ferraz, Marcia de A.M.M.</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chan%2C+Biankha%22">Chan, Biankha</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mastromonaco%2C+Gabriela+F%2E%22">Mastromonaco, Gabriela F.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> gmastromonaco@torontozoo.ca</i>
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  Data: <searchLink fieldCode="JN" term="%22Biochemistry+%26+Cell+Biology%22">Biochemistry & Cell Biology</searchLink>. 5/25/2026, Vol. 104, p1-14. 14p.
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  Data: *<searchLink fieldCode="DE" term="%22Fibroblasts%22">Fibroblasts</searchLink><br />*<searchLink fieldCode="DE" term="%22Epigenetics%22">Epigenetics</searchLink><br />*<searchLink fieldCode="DE" term="%22Chromatin%22">Chromatin</searchLink><br />*<searchLink fieldCode="DE" term="%22Somatic+cell+nuclear+transfer%22">Somatic cell nuclear transfer</searchLink><br />*<searchLink fieldCode="DE" term="%22Reproductive+technology%22">Reproductive technology</searchLink><br />*<searchLink fieldCode="DE" term="%22DNA+methylation%22">DNA methylation</searchLink><br />*<searchLink fieldCode="DE" term="%22Bioenergetics%22">Bioenergetics</searchLink>
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  Data: Somatic cell-based reproductive technologies (SCRTs) offer a promising approach for preserving the genetic diversity of threatened species. SCRTs require the nuclear reprogramming of a donor somatic cell, which has low success rates (1%–5%) and can vary significantly between individuals and cell lines derived from the same individual. Somatic and pluripotent cells differ in several key characteristics, and donor cells presenting more pluripotent-like traits have been found to result in improved reprogramming outcomes. Here, we characterized 18 standardized fibroblast cell lines from six Angus bulls for several of these characteristics at the inter- and intra-individual levels: bioenergetic status, targeted metabolite abundance, global DNA methylation levels, and presence of key histone modifications. Differences in the metaboloepigenetic profiles of these cell lines were observed at both inter- and intra-individual levels. By integrating these data, we positioned cell lines along a somatic-to-pluripotent-like continuum and identified a reduced set of features that captured the dominant separation between profiles. Although functional reprogramming outcomes were not assessed here, these results provide a data-driven framework to prioritise donor cell lines for prospective induced pluripotent stem cell/somatic cell nuclear transfer validation and to guide the development of practical biomarker panels for SCRT workflows. [ABSTRACT FROM AUTHOR]
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RecordInfo BibRecord:
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    Identifiers:
      – Type: doi
        Value: 10.1139/bcb-2025-0438
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 14
        StartPage: 1
    Subjects:
      – SubjectFull: Fibroblasts
        Type: general
      – SubjectFull: Epigenetics
        Type: general
      – SubjectFull: Chromatin
        Type: general
      – SubjectFull: Somatic cell nuclear transfer
        Type: general
      – SubjectFull: Reproductive technology
        Type: general
      – SubjectFull: DNA methylation
        Type: general
      – SubjectFull: Bioenergetics
        Type: general
    Titles:
      – TitleFull: Exploring metaboloepigenetic variability in bovine fibroblast lines.
        Type: main
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      – PersonEntity:
          Name:
            NameFull: Hawrylyshyn, Eric
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            NameFull: Ferraz, Marcia de A.M.M.
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            NameFull: Chan, Biankha
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            NameFull: Mastromonaco, Gabriela F.
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          Dates:
            – D: 25
              M: 05
              Text: 5/25/2026
              Type: published
              Y: 2026
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            – Type: issn-print
              Value: 08298211
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            – Type: volume
              Value: 104
          Titles:
            – TitleFull: Biochemistry & Cell Biology
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