Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Legionella detection and enumeration in water samples by ISO 11731-2017: which method is the most sensitive? |
| Συγγραφείς: |
Grandbastien, Bruno1, Mauffrey, Florian1, Greub, Gilbert2, Blanc, Dominique S.1,2 dominique.blanc@chuv.ch |
| Πηγή: |
Applied & Environmental Microbiology. Nov2025, Vol. 91 Issue 11, p1-7. 7p. |
| Θεματικοί όροι: |
*Legionella, *Water sampling, *Pneumonia, *Sensitivity & specificity (Statistics), *Detection algorithms, *Health care industry |
| Εταιρία/Οντότητα: |
International Organization for Standardization |
| Περίληψη: |
Legionnaires' disease is a severe pneumonia caused by inhalation of Legionella-contaminated aerosols. The prevention of Legionella relies on water disinfection and on accurate water testing to assess the effectiveness of these interventions. The norm ISO 11731:2017 provides multiple culture-based methods for detecting Legionella in water and recommends taking into account the most unfavorable result. This study evaluated six ISO-standardized techniques, including variations in media, concentration, and pre-treatments (heat or acid), applied to 276 hospital hot water samples. Legionella was detected in 120 samples, with substantial variability across methods. The most sensitive single technique (76%) was concentration and elution with membrane ane filte r on GVPC medium after heat treatment. Combinations of three techniques yielded a higher detection rate (98%), while using all six techniques detected all positive samples. Techniques involving concentration and elution proved superior to direct plating or simple filtration. Heat treatment enhanced detection more effectively than acid treatment, especially in samples with interfering flora. GVPC medium consistently outperformed BCYE. The study supports using a strategic combination of methods, particularly those involving membrane filtration and GVPC medium with heat treatment, to optimize Legionella detection in healthcare water systems. [ABSTRACT FROM AUTHOR] |
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