Academic Journal
Προβολή σε EDS

Anti-HIV-1 Effect of the Fluoroquinolone Enoxacin and Modulation of Pro-Viral hsa-miR-132 Processing in CEM-SS Cells.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Anti-HIV-1 Effect of the Fluoroquinolone Enoxacin and Modulation of Pro-Viral hsa-miR-132 Processing in CEM-SS Cells.
Συγγραφείς: Schlösser, Verena1 (AUTHOR), Lightfoot, Helen Louise1 (AUTHOR), Leemann, Christine2,3 (AUTHOR), Bejoy, Aathma Merin4 (AUTHOR), Tiwari, Shashank4 (AUTHOR), Schloßhauer, Jeffrey L.4 (AUTHOR), Vongrad, Valentina2,3 (AUTHOR), Brunschweiger, Andreas1,5 (AUTHOR), Hall, Jonathan1 (AUTHOR), Metzner, Karin J.2,3 (AUTHOR) karin.metzner@usz.ch, Imig, Jochen1,4 (AUTHOR) jochen.imig@mpi-dortmund.mpg.de
Πηγή: Non-Coding RNA. Feb2025, Vol. 11 Issue 1, p8. 21p.
Θεματικοί όροι: *Small interfering RNA, *RNA interference, *MicroRNA, *Antiretroviral agents, *Gene transfection
Περίληψη: Background: Despite tremendous advances in antiretroviral therapy (ART) against HIV-1 infections, no cure or vaccination is available. Therefore, discovering novel therapeutic strategies remains an urgent need. In that sense, miRNAs and miRNA therapeutics have moved intensively into the focus of recent HIV-1-related investigations. A strong reciprocal interdependence has been demonstrated between HIV-1 infection and changes of the intrinsic cellular miRNA milieu. This interrelationship may direct potential alterations of the host cells' environment beneficial for the virus or its suppression of replication. Whether this tightly balanced and controlled battle can be exploited therapeutically remains to be further addressed. In this context, the fluoroquinolone antibiotic Enoxacin has been demonstrated as a potent modulator of miRNA processing. Here, we test the hypothesis that this applies also to selected HIV-1-related miRNAs. Methods: We studied the effect of Enoxacin on HIV-1 replication coupled with miRNA qRT-PCR analysis of HIV-1-related miRNAs in CEM-SS and MT-4 T-cells. The effects of miRNA mimic transfections combined with Enoxacin treatment on HIV-1 replication were assessed. Finally, we employed an in vitro DICER1 cleavage assay to study the effects of Enoxacin on a pro-HIV-1 miRNA hsa-miR-132 processing. Results: We established that Enoxacin, but not the structurally similar compound nalidixic acid, exhibits strong anti-HIV-1 effects in the T-cell line CEM-SS, but not MT-4. We provide experimental data that this effect of Enoxacin is partly attributed to the specific downregulation of mature hsa-miR-132-3p, but not other tested pro- or anti-HIV-1 miRNAs, which is likely due to affecting DICER1 processing. Conclusions: Our findings show an anti-retroviral activity of Enoxacin at least in part by downregulation of hsa-miR-132-3p, which may be relevant for future antiviral therapeutic applications by modulation of the RNA interference pathway. [ABSTRACT FROM AUTHOR]
Βάση Δεδομένων: Academic Search Index
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://resolver.ebsco.com/c/fiv2js/result?sid=EBSCO:asx&genre=article&issn=2311553X&ISBN=&volume=11&issue=1&date=20250201&spage=8&pages=8-28&title=Non-Coding RNA&atitle=Anti-HIV-1%20Effect%20of%20the%20Fluoroquinolone%20Enoxacin%20and%20Modulation%20of%20Pro-Viral%20hsa-miR-132%20Processing%20in%20CEM-SS%20Cells.&aulast=Schl%C3%B6sser%2C%20Verena&id=DOI:10.3390/ncrna11010008
    Name: Full Text Finder (for New FTF UI) (ns324271)
    Category: fullText
    Text: Full Text Finder
    MouseOverText: Full Text Finder
Header DbId: asx
DbLabel: Academic Search Index
An: 183335835
RelevancyScore: 1354
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 1353.69750976563
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Anti-HIV-1 Effect of the Fluoroquinolone Enoxacin and Modulation of Pro-Viral hsa-miR-132 Processing in CEM-SS Cells.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Schlösser%2C+Verena%22">Schlösser, Verena</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lightfoot%2C+Helen+Louise%22">Lightfoot, Helen Louise</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Leemann%2C+Christine%22">Leemann, Christine</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bejoy%2C+Aathma+Merin%22">Bejoy, Aathma Merin</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tiwari%2C+Shashank%22">Tiwari, Shashank</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Schloßhauer%2C+Jeffrey+L%2E%22">Schloßhauer, Jeffrey L.</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Vongrad%2C+Valentina%22">Vongrad, Valentina</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Brunschweiger%2C+Andreas%22">Brunschweiger, Andreas</searchLink><relatesTo>1,5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hall%2C+Jonathan%22">Hall, Jonathan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Metzner%2C+Karin+J%2E%22">Metzner, Karin J.</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<i> karin.metzner@usz.ch</i><br /><searchLink fieldCode="AR" term="%22Imig%2C+Jochen%22">Imig, Jochen</searchLink><relatesTo>1,4</relatesTo> (AUTHOR)<i> jochen.imig@mpi-dortmund.mpg.de</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Non-Coding+RNA%22">Non-Coding RNA</searchLink>. Feb2025, Vol. 11 Issue 1, p8. 21p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: *<searchLink fieldCode="DE" term="%22Small+interfering+RNA%22">Small interfering RNA</searchLink><br />*<searchLink fieldCode="DE" term="%22RNA+interference%22">RNA interference</searchLink><br />*<searchLink fieldCode="DE" term="%22MicroRNA%22">MicroRNA</searchLink><br />*<searchLink fieldCode="DE" term="%22Antiretroviral+agents%22">Antiretroviral agents</searchLink><br />*<searchLink fieldCode="DE" term="%22Gene+transfection%22">Gene transfection</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Background: Despite tremendous advances in antiretroviral therapy (ART) against HIV-1 infections, no cure or vaccination is available. Therefore, discovering novel therapeutic strategies remains an urgent need. In that sense, miRNAs and miRNA therapeutics have moved intensively into the focus of recent HIV-1-related investigations. A strong reciprocal interdependence has been demonstrated between HIV-1 infection and changes of the intrinsic cellular miRNA milieu. This interrelationship may direct potential alterations of the host cells' environment beneficial for the virus or its suppression of replication. Whether this tightly balanced and controlled battle can be exploited therapeutically remains to be further addressed. In this context, the fluoroquinolone antibiotic Enoxacin has been demonstrated as a potent modulator of miRNA processing. Here, we test the hypothesis that this applies also to selected HIV-1-related miRNAs. Methods: We studied the effect of Enoxacin on HIV-1 replication coupled with miRNA qRT-PCR analysis of HIV-1-related miRNAs in CEM-SS and MT-4 T-cells. The effects of miRNA mimic transfections combined with Enoxacin treatment on HIV-1 replication were assessed. Finally, we employed an in vitro DICER1 cleavage assay to study the effects of Enoxacin on a pro-HIV-1 miRNA hsa-miR-132 processing. Results: We established that Enoxacin, but not the structurally similar compound nalidixic acid, exhibits strong anti-HIV-1 effects in the T-cell line CEM-SS, but not MT-4. We provide experimental data that this effect of Enoxacin is partly attributed to the specific downregulation of mature hsa-miR-132-3p, but not other tested pro- or anti-HIV-1 miRNAs, which is likely due to affecting DICER1 processing. Conclusions: Our findings show an anti-retroviral activity of Enoxacin at least in part by downregulation of hsa-miR-132-3p, which may be relevant for future antiviral therapeutic applications by modulation of the RNA interference pathway. [ABSTRACT FROM AUTHOR]
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asx&AN=183335835
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.3390/ncrna11010008
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 21
        StartPage: 8
    Subjects:
      – SubjectFull: Small interfering RNA
        Type: general
      – SubjectFull: RNA interference
        Type: general
      – SubjectFull: MicroRNA
        Type: general
      – SubjectFull: Antiretroviral agents
        Type: general
      – SubjectFull: Gene transfection
        Type: general
    Titles:
      – TitleFull: Anti-HIV-1 Effect of the Fluoroquinolone Enoxacin and Modulation of Pro-Viral hsa-miR-132 Processing in CEM-SS Cells.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Schlösser, Verena
      – PersonEntity:
          Name:
            NameFull: Lightfoot, Helen Louise
      – PersonEntity:
          Name:
            NameFull: Leemann, Christine
      – PersonEntity:
          Name:
            NameFull: Bejoy, Aathma Merin
      – PersonEntity:
          Name:
            NameFull: Tiwari, Shashank
      – PersonEntity:
          Name:
            NameFull: Schloßhauer, Jeffrey L.
      – PersonEntity:
          Name:
            NameFull: Vongrad, Valentina
      – PersonEntity:
          Name:
            NameFull: Brunschweiger, Andreas
      – PersonEntity:
          Name:
            NameFull: Hall, Jonathan
      – PersonEntity:
          Name:
            NameFull: Metzner, Karin J.
      – PersonEntity:
          Name:
            NameFull: Imig, Jochen
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 02
              Text: Feb2025
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-print
              Value: 2311553X
          Numbering:
            – Type: volume
              Value: 11
            – Type: issue
              Value: 1
          Titles:
            – TitleFull: Non-Coding RNA
              Type: main
ResultId 1