Εμφανίζονται 1 - 20 Αποτελέσματα από 77.651 για την αναζήτηση 'Urease blood', χρόνος αναζήτησης: 0,96δλ Περιορισμός αποτελεσμάτων
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    Academic Journal
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    Academic Journal

    Συγγραφείς: Bose, A., Biswas, K.

    Πηγή: IEEE Sensors Journal IEEE Sensors J. Sensors Journal, IEEE. 22(17):17275-17282 Sep, 2022

    Συνδεδεμένο Πλήρες Κείμενο
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    Electronic Resource

    Additional Titles: Two Legionella-like strains isolated from hot water distribution systems in 2012 have been characterized phenotypically, biochemically and genomically in terms of DNA relatedness. Both strains, HCPI-6T and EUR-108, exhibited biochemical phenotypic profiles typical of Legionella species. Cells were Gram-negative motile rods which grew on BCYEα agar but not on blood agar and displayed phenotypic characteristics typical of the family Legionellaceae, including a requirement for l-cysteine and testing catalase positive. Both strains were negative for oxidase, urease, nitrate reduction and hippurate negative, and non-fermentative. The major ubiquinone was Q12 (59.4 % HCPI-6T) and the dominant fatty acids were C16 : 1 ω7c (28.4 % HCPI-6T, ≈16 % EUR-108), C16 : 0 iso (≈22.5 % and ≈13 %) and C15 : 0 anteiso (19.5 % and ≈23.5 %, respectively). The percent G+C content of genomic DNA was determined to be 39.3 mol %. The 16S rRNA gene, mip sequence and comparative genome sequence-based analyses (average nucleotide identity, ANI; digital DNA–DNA hybridization, dDDH; and phylogenomic treeing) demonstrated that the strains represent a new species of the genus Legionella. The analysis based on the 16S rRNA gene sequences showed that the sequence similarities for both strains ranged from 98.8–90.1 % to other members of the genus. The core genome-based phylogenomic tree (protein-concatemer tree based on concatenation of 418 proteins present in single copy) revealed that these two strains clearly form a separate cluster within the genus Legionella. ANI and dDDH values confirmed the distinctiveness of the strains. Based on the genomic, genotypic and phenotypic findings from a polyphasic study, the isolates are considered to represent a single novel species, for which the name Legionella maioricensis sp. nov. is proposed. The type strain is HCPI-6T (=CCUG 75071T=CECT 30569T).

    Πηγή: Crespi , S , Drašar , V , Salvà-Serra , F , Jaén-Luchoro , D , Piñeiro-Iglesias , B , Lindemann , P C , Aliaga-Lozano , F , Fernández-Juárez , V , Coll-García , G , Moore , E R B & Bennasar-Figueras , A 2023 , ' Legionella maioricensis sp. nov., a new species isolated from the hot water distribution systems of a hospital and a shopping center during routine sampling ' , International Journal of Systematic and Evolutionary Microbiology , vol. 73 , no. 1 , 005686 .

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    Academic Journal

    Συγγραφείς: Kutlu, Nur1 (AUTHOR), İspirli Doğaç, Yasemin2 (AUTHOR) y-ispirli@hotmail.com, Deveci, İlyas3 (AUTHOR), Teke, Mustafa1 (AUTHOR)

    Πηγή: Preparative Biochemistry & Biotechnology. 2020, Vol. 50 Issue 5, p425-437. 13p. 3 Diagrams, 4 Charts, 8 Graphs.