The mass spectrometric intact transition epitope mapping method supports protein engineering of foldon trimer variants

Συγγραφείς: Cornelia Koy, Timo Zimmer, Kwabena F. M. Opuni, Armin Geyer, Michael O. Glocker

Πηγή: Sci Rep

Θεματικοί όροι: Models, Molecular, Protein Folding, Thermodynamics, Protein Multimerization, Protein Engineering, Article, Mass Spectrometry, Epitope Mapping

Σύνδεσμος πρόσβασης: https://pubmed.ncbi.nlm.nih.gov/41274901

Epitope Mapping of an Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody (EMab-51) Using the RIEDL Insertion for Epitope Mapping Method

Συγγραφείς: Ren Nanamiya, Masato Sano, Teizo Asano, Miyuki Yanaka, Takuro Nakamura, Masaki Saito, Tomohiro Tanaka, Hideki Hosono, Nami Tateyama, Mika K. Kaneko, Yukinari Kato

Πηγή: Monoclonal Antibodies in Immunodiagnosis & Immunotherapy. 40:149-155

Θεματικοί όροι: ErbB Receptors, 0301 basic medicine, Epitopes, 0303 health sciences, 03 medical and health sciences, Antibodies, Monoclonal, Antineoplastic Agents, Epitope Mapping, 3. Good health

Σύνδεσμος πρόσβασης: https://pubmed.ncbi.nlm.nih.gov/34424763

Development of a Novel Epitope Mapping System: RIEDL Insertion for Epitope Mapping Method

Συγγραφείς: Teizo Asano, Mika K. Kaneko, Yukinari Kato

Πηγή: Monoclonal Antibodies in Immunodiagnosis & Immunotherapy. 40:162-167

Θεματικοί όροι: 0301 basic medicine, Epitopes, 0303 health sciences, 03 medical and health sciences, Antibodies, Monoclonal, Flow Cytometry, Epitope Mapping

Σύνδεσμος πρόσβασης: https://pubmed.ncbi.nlm.nih.gov/34424761

Cryo-Electron Microscopy-Based Epitope Mapping

Συγγραφείς: Drulyte, Ieva, Hurdiss, Daniel L

Πηγή: Methods in Molecular Biology ISBN: 9781071645901

Θεματικοί όροι: Epitopes, SDG 3 - Good Health and Well-being, Antigen-Antibody Complex/immunology, Cryoelectron Microscopy, Epitope Mapping/methods, Humans, Epitopes/immunology, Cryoelectron Microscopy/methods, Antigen-Antibody Complex, Epitope Mapping

Σύνδεσμος πρόσβασης: https://pubmed.ncbi.nlm.nih.gov/40593414

Simultaneous polyclonal antibody sequencing and epitope mapping by cryo electron microscopy and mass spectrometry

Συγγραφείς: Schulte, Douwe, Šiborová, Marta, Käll, Lukas, Snijder, Joost

Πηγή: eLife
eLife, Vol 14 (2025)

Θεματικοί όροι: Proteomics, QH301-705.5, Science, Cryoelectron Microscopy/methods, Tandem Mass Spectrometry/methods, Antibodies, proteomics, Immunology and Inflammation, Tandem Mass Spectrometry, Sequence Analysis, Protein, antibody, Humans, Biology (General), Chromatography, Liquid, epitope, Cryoelectron Microscopy, repertoire, cryoEM, Epitope Mapping/methods, Medicine, Proteomics/methods, Protein/methods, Antibodies/chemistry, Sequence Analysis, Epitope Mapping, Chromatography, Liquid

Σύνδεσμος πρόσβασης: https://pubmed.ncbi.nlm.nih.gov/40266252
https://doaj.org/article/dacc2b379ef0412fb4311005f9cc6368

Epitope mapping of an anti-human epidermal growth factor receptor monoclonal antibody (EMab-51) using the RIEDL insertion for epitope mapping method.

Additional Titles: 免疫アレルギー学

Όροι ευρετηρίου: EGFR, EMab-51, epitope mapping, monoclonal antibody, RIEDL tag, Immunology and Allergy, Immunology, Journal Article.

Σύνδεσμος: https://doi.org/10.1089/mab.2021.0010
2167-9436

B 细胞抗原表位鉴定方法的研究进展.

Alternate Title: Advances on research of B-cell epitopes mapping methods.

Συγγραφείς: 伍春平¹ wu0502mailchinese@163.com, 尹苗¹, 陈希文¹ xwch05@163.com

Πηγή: Chinese Journal of Immunology. Jun2024, Vol. 40 Issue 6, p1329-1334. 6p.

T cell epitope identification for bovine vaccines: an epitope mapping method for BoLA A-11

Συγγραφείς: Groot, A.S., Nene, Vishvanath, Hegde, N.R., Srikumaran, S., Rayner , J., Martin, W.

Πηγή: International Journal for Parasitology. 33:641-653

Θεματικοί όροι: lymphocytes, 0301 basic medicine, Vaccines, bovinae, Epitopes, T-Lymphocyte, vaccines, Ligands, Models, Biological, major histocompatibility complex, 3. Good health, Major Histocompatibility Complex, 03 medical and health sciences, 0302 clinical medicine, cattle, antigens, Sequence Analysis, Protein, Animals, Humans, Cattle, Algorithms, Epitope Mapping

Περιγραφή αρχείου: p. 641-653

Σύνδεσμος πρόσβασης: https://pubmed.ncbi.nlm.nih.gov/12782061

Development of a novel epitope mapping method (REMAP) using RIEDL tag system

Συγγραφείς: Teizo, Asano, primary, Kaneko, Mika, additional, Kato, Yukinari, additional

Πηγή: Proceedings for Annual Meeting of The Japanese Pharmacological Society. 2021/01/01

Σύνδεσμος πρόσβασης: https://www.jstage.jst.go.jp/article/jpssuppl/94/0/94_1-Y-E3-3/_pdf

Intact Transition Epitope Mapping - Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE)

Additional Titles: Epitope mapping, which is the identification of antigenic determinants, is essential for the design of novel antibody-based therapeutics and diagnostic tools. ITEM-THREE is a mass spectrometry-based epitope mapping method that can identify epitopes on antigens upon generating an immune complex in electrospray-compatible solutions by adding an antibody of interest to a mixture of peptides from which at least one holds the antibody's epitope. This mixture is nano-electrosprayed without purification. Identification of the epitope peptide is performed within a mass spectrometer that provides an ion mobility cell sandwiched in-between two collision cells and where this ion manipulation setup is flanked by a quadrupole mass analyzer on one side and a time-of-flight mass analyzer on the other side. In a stepwise fashion, immune-complex ions are separated from unbound peptide ions and dissociated to release epitope peptide ions. Immune complex-released peptide ions are separated from antibody ions and fragmented by collision induced dissociation. Epitope-containing peptide fragment ions are recorded, and mass lists are submitted to unsupervised data base search thereby retrieving both, the amino acid sequence of the epitope peptide and the originating antigen. ITEM-THREE was developed with antiTRIM21 and antiRA33 antibodies for which the epitopes were known, subjecting them to mixtures of synthetic peptides of which one contained the respective epitope. ITEM-THREE was then successfully tested with an enzymatic digest of His-tagged recombinant human β-actin and an antiHis-tag antibody, as well as with an enzymatic digest of recombinant human TNFα and an antiTNFα antibody whose epitope was previously unknown.

Συγγραφείς: Danquah, Bright D., Röwer, Claudia, Opuni, Kwabena F. M., El-Kased, Reham, Frommholz, David, Illges, Harald, Koy, Cornelia, Glocker, Michael O.

Πηγή: Molecular & Cellular Proteomics; ISSN 1535-9476; Molecular & Cellular Proteomics 18, 1543–1555

Όροι ευρετηρίου: ddc:570, article, doc-type:article, publishedVersion

Σύνδεσμος: https://pub.h-brs.de/frontdoor/index/index/docId/4563
info:eu-repo/semantics/altIdentifier/doi/10.1074/mcp.RA119.001429
info:eu-repo/semantics/altIdentifier/pmid/31147491

Characterization and epitope mapping of monoclonal antibodies against PEDV N protein

Συγγραφείς: Zhao, Yongxiang ^{a, b}, Fan, Baochao ^{a, b}, Xue, Junbiao ^c, Guo, Rongli ^{a, b}, Li, Jizhong ^{a, b}, Zhou, Jinzhu ^{a, b}, Song, Xu ^{a, e}, Zhang, Xuehan ^{a, b}, Tao, Shengce ^{c, ∗∗}, Li, Bin ^{a, b, d, ∗}

Πηγή: In Virology February 2023 579:29-37

Epitope mapping via in vitro deep mutational scanning methods and its applications

Συγγραφείς: Keen, Meredith M., Keith, Alasdair D., Ortlund, Eric A. ^∗

Πηγή: In Journal of Biological Chemistry

Mapping of Surface-Exposed Epitopes of In Vitro and In Vivo Aggregated Species of Alpha-Synuclein

Συγγραφείς: Almandoz-Gil, Leire, Lindström, Veronica, Sigvardson, Jessica, Kahle, Philipp J., Lannfelt, Lars, Ingelsson, Martin, Bergström, Joakim

Πηγή: Cell Mol Neurobiol
Cellular and molecular neurobiology 37(7), 1217-1226 (2017). doi:10.1007/s10571-016-0454-0

Θεματικοί όροι: Male, 0301 basic medicine, alpha-Synuclein: metabolism, alpha-Synuclein: genetics, Parkinson's disease, Dementia with Lewy bodies, Epitope mapping, Geriatrik, Mice, Transgenic, Epitopes: metabolism, Alpha-synuclein, Cellular and Molecular Neuroscience, Epitopes, Mice, Protein Aggregates, 03 medical and health sciences, Animals, Humans, Amino Acid Sequence, Original Research, Aged, 80 and over, 2. Zero hunger, 0303 health sciences, Epitopes: genetics, Brain, Chromosome Mapping, Cell Biology, Middle Aged, Protein Aggregates: physiology, 3. Good health, Mice, Inbred C57BL, Brain: pathology, Geriatrics, Chromosome Mapping: methods, alpha-Synuclein, Female, Brain: metabolism

Περιγραφή αρχείου: application/pdf

Σύνδεσμος πρόσβασης: https://link.springer.com/content/pdf/10.1007%2Fs10571-016-0454-0.pdf
https://pubmed.ncbi.nlm.nih.gov/28028735
https://pub.dzne.de/record/139489
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-334384

Identification of Candidate Immunodominant Epitopes and Their HLA-Binding Prediction on BK Polyomavirus Proteins in Healthy Donors.

Συγγραφείς: Lara-de-León AG; Advanced & Cell Therapy Services, Banc de Sang i Teixits (Blood and Tissue Bank, BST), Barcelona, Spain., Mora-Buch R; Advanced & Cell Therapy Services, Banc de Sang i Teixits (Blood and Tissue Bank, BST), Barcelona, Spain.; Transfusional Medicine Group, Vall d'Hebron Research Institute, Universitat Autònoma de Barcelona (VHIR-UAB), Barcelona, Spain., Cantó E; Advanced & Cell Therapy Services, Banc de Sang i Teixits (Blood and Tissue Bank, BST), Barcelona, Spain.; Transfusional Medicine Group, Vall d'Hebron Research Institute, Universitat Autònoma de Barcelona (VHIR-UAB), Barcelona, Spain., Peña-Gómez C; Mental Health and Neurosciences, Mixt Unit, Parc Taulí Research and Innovation Institute (I3PT), Barcelona, Spain., Rudilla F; Transfusional Medicine Group, Vall d'Hebron Research Institute, Universitat Autònoma de Barcelona (VHIR-UAB), Barcelona, Spain.; Immunogenetics and Histocompatibility Laboratory, Banc de Sang i Teixits (Blood and Tissue Bank, BST), Barcelona, Spain.

Πηγή: HLA [HLA] 2024 Oct; Vol. 104 (4), pp. e15722.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Wiley Blackwell Country of Publication: England NLM ID: 101675570 Publication Model: Print Cited Medium: Internet ISSN: 2059-2310 (Electronic) Linking ISSN: 20592302 NLM ISO Abbreviation: HLA Subsets: MEDLINE

Ιατρικοί όροι (MeSH): BK Virus*/immunology , Immunodominant Epitopes*/immunology, Epitopes, T-Lymphocyte/immunology ; Polyomavirus Infections/immunology ; Polyomavirus Infections/virology ; HLA Antigens/immunology ; Viral Proteins/immunology ; Viral Proteins/chemistry ; Humans ; Protein Binding ; Epitope Mapping ; Healthy Volunteers ; Male ; Female ; Adult ; Alleles

Snapshot imprinting: rapid identification of cancer cell surface proteins and epitopes using molecularly imprinted polymers

Συγγραφείς: Piletsky, Stanislav S. ^{a, ⁎}, Piletska, Elena ^b, Poblocka, Marta ^c, Macip, Salvador ^{c, d}, Jones, Donald J.L. ^{e, f, g}, Braga, Marta ^h, Cao, Thong H. ^{f, g}, Singh, Rajinder ^e, Spivey, Alan C. ^a, Aboagye, Eric O. ^h, Piletsky, Sergey A. ^b

Πηγή: In Nano Today December 2021 41

An Overview of Epitopes and Methods for Antibody Epitope Mapping.

Συγγραφείς: Nilvebrant J; School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden., Berndt Thalén N; School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden., Rockberg J; School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden. johanr@biotech.kth.se., Malm M; School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden. magdalena.malm@biotech.kth.se.

Πηγή: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2937, pp. 1-11.

Τύπος έκδοσης: Journal Article; Review

Στοιχεία περιοδικού: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Epitope Mapping*/methods , Epitopes*/immunology , Epitopes*/chemistry , Epitopes*/genetics , Antibodies*/immunology , Antibodies*/chemistry, Computational Biology/methods ; Antigens/immunology ; Antigens/chemistry ; Humans ; Animals

Epitope Mapping and Fine Specificity of Human T and B Cell Responses for Novel Candidate Blood-Stage Malaria Vaccine P27A

Συγγραφείς: Kristina M. Geiger, Daniel Guignard, Che Yang, Jean-Pierre Bikorimana, Bruno E. Correia, Sophie Houard, Catherine Mkindi, Claudia A. Daubenberger, François Spertini, Giampietro Corradin, Régine Audran

Πηγή: Front Immunol
Frontiers in Immunology, Vol 11 (2020)
Frontiers in immunology.-2020
Frontiers in immunology, vol. 11, pp. 412

Θεματικοί όροι: Adult, 0301 basic medicine, Adjuvants, Immunologic, Antibodies, Protozoan/metabolism, Antibody Affinity, Antigens, Protozoan/genetics, Antigens, Protozoan/immunology, Epitope Mapping/methods, Epitopes, B-Lymphocyte/genetics, Epitopes, B-Lymphocyte/immunology, Epitopes, T-Lymphocyte/genetics, Epitopes, T-Lymphocyte/immunology, Humans, Life Cycle Stages, Lymphocyte Activation, Malaria Vaccines/immunology, Malaria, Falciparum/immunology, Peptides/genetics, Peptides/immunology, Plasmodium falciparum, Protozoan Proteins/genetics, Protozoan Proteins/immunology, Switzerland, Tanzania, Vaccination, P27A, adjuvant, clinical trial, immune response, malaria, populations, vaccine, Immunology, Protozoan Proteins, Antibodies, Protozoan, Epitopes, T-Lymphocyte, Antigens, Protozoan, 03 medical and health sciences, Malaria Vaccines, Malaria, Falciparum, RC581-607, 3. Good health, Epitopes, B-Lymphocyte, Immunologic diseases. Allergy, Peptides, Epitope Mapping

Περιγραφή αρχείου: application/pdf

Σύνδεσμος πρόσβασης: https://www.frontiersin.org/articles/10.3389/fimmu.2020.00412/pdf
https://pubmed.ncbi.nlm.nih.gov/32210975
https://doaj.org/article/8aa2e3adbc2a4cbca7e1e48344279cbc
https://sonar.ch/global/documents/172983
https://serval.unil.ch/resource/serval:BIB_941F355259B5.P001/REF.pdf
https://serval.unil.ch/notice/serval:BIB_941F355259B5
http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_941F355259B52

Automatic Generation of Validated Specific Epitope Sets

Συγγραφείς: Sebastian Carrasco Pro, John Sidney, Sinu Paul, Cecilia S. Lindestam Arlehamn, Daniela Weiskopf, Bjoern Peters, Alessandro Sette

Πηγή: J Immunol Res
Journal of Immunology Research, Vol 2015 (2015)

Θεματικοί όροι: 0301 basic medicine, Herpesvirus 4, Human, Databases, Factual, Epitopes, T-Lymphocyte/metabolism, T-Lymphocytes, Epitopes, T-Lymphocyte, Lymphocyte Activation, Prediction of Peptide-MHC Binding Affinity, Computational biology, Context (archaeology), 0302 clinical medicine, Precision Medicine, Mycobacteriaceae, Cells, Cultured, Immunology and Microbiology, Dengue Virus/immunology, Life Sciences, 3. Good health, Antigen, Mycobacteriaceae/immunology, Immune Responses, Epitope, Immunotherapy, Research Article, Herpesvirus 4, Human/immunology, T-Cell Epitopes, Immunobiology of Dendritic Cells, Immunology, Immunologic Tests, Viral Proteins, 03 medical and health sciences, Biochemistry, Genetics and Molecular Biology, Virology, Humans, Molecular Biology, Biology, Electronic Data Processing, FOS: Clinical medicine, T cell, Paleontology, T-Lymphocytes/immunology, RC581-607, Dengue Virus, Computer science, Epitope Mapping/methods, Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome, Viral Proteins/immunology, Epitope Prediction, Immunologic diseases. Allergy, B-Cell Epitopes, Epitope Mapping, Software

Περιγραφή αρχείου: text/xhtml

Σύνδεσμος πρόσβασης: http://downloads.hindawi.com/journals/jir/2015/763461.pdf
https://pubmed.ncbi.nlm.nih.gov/26568965
https://doaj.org/article/f64e530d503d4d40bbf6209b232b1c7c
https://hdl.handle.net/20.500.12866/5352
https://doi.org/10.1155/2015/763461

Identification of the epitope in human poliovirus type 1 Sabin strain recognized by the monoclonal antibody 1G10 using mimotope strategy

Συγγραφείς: Bai, Han ^a, Liu, Shaohua ^a, Shi, Shenghe ^b, Lu, Weiwei ^a, Yang, Yongjuan ^a, Zhu, Yunkai ^a, Zhang, Zhongyang ^a, Guo, Huijie ^a, Li, Xiuling ^{a, *}

Πηγή: In Journal of Virological Methods February 2020 276

Epitope Mapping with Sidewinder : An XL-MS and Structural Modeling Approach

Συγγραφείς: Ströbaek, Joel, Tang, Di, Gueto-Tettay, Carlos, Gomez Toledo, Alejandro, Olofsson, Berit, Hartman, Erik, Heusel, Moritz, Malmström, Johan, Malmström, Lars

Πηγή: International Journal of Molecular Sciences; 26(4), pp 1-17 (2025) ; ISSN: 1422-0067

Θεματικοί όροι: Bioinformatics (Computational Biology), Epitope Mapping/methods, Streptococcus pyogenes/immunology, Bacterial Proteins/chemistry, Molecular Docking Simulation, Antibodies, Monoclonal/chemistry, Mass Spectrometry/methods, Epitopes/immunology, Streptolysins/chemistry, Bacterial/immunology

Relation: http://dx.doi.org/10.3390/ijms26041488; pmid:40003954; scopus:85218901966

Διαθεσιμότητα: https://lup.lub.lu.se/record/18960a88-0ea4-4ce7-a6c1-34132a89496d
https://doi.org/10.3390/ijms26041488