Optimization of an untargeted method for metabolomics and lipidomics profiling of cells using GC×GC-TOFMS

Authors: Bensaada, Djulia, Albendea, Paula, Rodrigues, Anaïs, Pierret, Damien, Dejong, Thibaut, Maillard, Bastien, Focant, Jean-François, Stefanuto, Pierre-Hugues, Purcaro, Giorgia

Source: 3rd advance in separation sciences, Gembloux, Belgium [BE], June 12-13 2025

Subject Terms: Metabolomics, GC×GC, GC×GC-TOFMS, MSTFA, BSTFA, Lipidomics, Fames, Microwave, TMSH, BF3, Physical, chemical, mathematical & earth Sciences, Chemistry, Physique, chimie, mathématiques & sciences de la terre, Chimie

Access URL: https://orbi.uliege.be/handle/2268/334180

Spontaneous dehydration side reactions of core amino acids in GC-MS sample preparation: the role of drying conditions

Authors: Karnaeva, Anastasia E.^{j_pac-2024-0328_aff_001}, Korzhenevskii, Dmitry A.^{j_pac-2024-0328_aff_002}, Nesterenko, Alexey M.^{j_pac-2024-0328_aff_002}

Source: Pure and Applied Chemistry. 97(4):373-380

Availability: https://www.degruyter.com/document/doi/10.1515/pac-2024-0328/html

Optimizing Solvents and Derivatizing Agents for Metabolomic Profiling of Human Plasma Using GC–MS

Authors: Srivastava, Anshuman^Aff1, Mishra, Alok^Aff1, Shrivastava, Ashutosh^{Aff1, IDs1033702304266z_cor3}

Source: Chromatographia: An International Journal for Separation Science. 86(7):523-534

Dispersive solid-phase extraction for simultaneous determination of four amphetamines drugs in urine using gas chromatography–mass spectrometry

Authors: Elboraie, Mahir Z.^Aff1, Elbashir, Abdalla A.^{Aff1, Aff2, IDs13738021023390_cor2}, Eid, Eltayeb E. M.^Aff3, Aboul-Enein, Hassan Y.^{Aff4, IDs13738021023390_cor4}

Source: Journal of the Iranian Chemical Society. 19(3):753-762

Development and validation of the method for the detection of glimepiride via derivatization employing N-methyl-N-(trimethylsilyl) trifluoroacetamide using gas chromatography-mass spectrometry

Authors: Priyanka Verma, Atul Bajaj, R. M. Tripathi, Sudhir K. Shukla, Suman Nagpal

Source: Egyptian Journal of Forensic Sciences, Vol 11, Iss 1, Pp 1-9 (2021)

Subject Terms: Diabetes mellitus, Glimepiride, MSTFA, Derivatization, Gas chromatography-mass spectrometry, Biological matrices, Law in general. Comparative and uniform law. Jurisprudence, K1-7720, Medicine (General), R5-920

File Description: electronic resource

Relation: https://doaj.org/toc/2090-5939

Access URL: https://doaj.org/article/e20b55446c324fa89eb6c708767c0cd6

Sugars in environmental samples and sedimentary rocks – effectiveness in derivatization for GC-MS analysis

Authors: Smolarek-Lach, Justyna, Goryl, Magdalena, Marynowski, Leszek

Source: Geological Quarterly; Vol. 65 No. 3 (2021); 65: 40, doi:10.7306/gq.1609 ; Geological Quarterly; Tom 65 Nr 3 (2021); 65: 40, doi:10.7306/gq.1609 ; 1641-7291

Subject Terms: BSTFA, MSTFA, particulate matter, fungi, lignites

File Description: application/pdf

Relation: https://gq.pgi.gov.pl/article/view/32955/pdf; https://gq.pgi.gov.pl/article/view/32955

Availability: https://gq.pgi.gov.pl/article/view/32955

Mixtures of skew-[formula omitted] factor analyzers

Authors: Murray, Paula M., Browne, Ryan P., McNicholas, Paul D. ^⁎

Source: In Computational Statistics and Data Analysis September 2014 77:326-335

Extraction of bisphenol-A and 17β-estradiol from water samples via solid-phase extraction (SPE)

Authors: Kim, Won-Seok^{aff1_w2aab3b7c50b1b6b1aab1b4b4Aa}, Do, Anh^{aff1_w2aab3b7c50b1b6b1aab1b4b4Aa}, Yeh, Daniel^{aff1_w2aab3b7c50b1b6b1aab1b4b4Aa}, Cunningham, Jeffrey^{aff1_w2aab3b7c50b1b6b1aab1b4b4Aa}

Source: Reviews in Analytical Chemistry. 33(1):59-77

Availability: https://www.degruyter.com/document/doi/10.1515/revac-2013-0016/html

Determination of oxygenated compounds in secondary organic aerosol from isoprene and toluene smog chamber experiments

Authors: Borrás García, Esther Mª, Tortajada-Genaro, Luis Antonio|||0000-0003-4021-5607

Contributors: Departamento de Química, Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural, Ministerio de Educación y Ciencia, Generalitat Valenciana, Fundación Bancaja, Repositorio Institucional de la Universitat Politècnica de València Riunet

Source: RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Universitat Politècnica de València (UPV)
instname

Subject Terms: Atmospheric chemistry, Isoprene, Oxygenated compounds, Secondary organic aerosols, Secondary organic aerosol, Smog chambers, Analytical strategy, P-cresol, 01 natural sciences, Nitrophenols, Degradation, MSTFA, Phenols, QUIMICA ANALITICA, Simulation chambers, Chemical analysis, Benzoic acid, Anthropogenic pollutants, 0105 earth and related environmental sciences, Aldehydes, Gas chromatography, Signal to noise ratio, Mass spectrometry, Carboxylic acids, Signal to noise, Aerosol samples, Glyoxylic acids, PFBHA, Atmospheric aerosols, Multifunctional compounds, 3. Good health, 2-methylglyceric acid, 13. Climate action, Gas chromatography-mass spectrometry, Photoreactors, Derivatisation, Atmospheric degradation, Degradation products, Toluene

File Description: application/pdf

Access URL: https://riunet.upv.es/bitstream/10251/66088/2/Revised_manuscript.pdf
https://riunet.upv.es/handle/10251/66088

Accelerated sample treatment for screening of banned doping substances by GC–MS: ultrasonication versus microwave energy

Authors: M. Galesio, M. Mazzarino, X. De La Torre, BOTRE', Francesco, J. L. Capelo

Source: Analytical and Bioanalytical Chemistry. 399:861-875

Subject Terms: Substance Abuse Detection, 0301 basic medicine, 03 medical and health sciences, Humans, Ultrasonics, Urinalysis, Microwaves, microwave energy, doping substances, anti-doping, gc-ms, gc–ms, mstfa derivatisation, ultrasonic energy, sample treatment, β-glucuronidase hydrolysis, 01 natural sciences, Gas Chromatography-Mass Spectrometry, 0104 chemical sciences

File Description: application/pdf

Access URL: https://pubmed.ncbi.nlm.nih.gov/21049269
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000285781900033&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a
https://doi.org/10.1007/s00216-010-4319-y
https://hdl.handle.net/11573/402128
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000285781900033&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a
https://doi.org/10.1007/s00216-010-4319-y
https://hdl.handle.net/2158/1418756

Metabolomic analysis of placenta tissue in obese pregnancies

Authors: C. Fattuoni, C. Mandò, F. Palmas, G. M. Anelli, C. Novielli, E. Parejo Laudicina, V. M. Savasi, L. Barberini, A. Dessì, R. Pintus, V. Fanos, A. Noto, I. Cetin

Contributors: C. Fattuoni, C. Mandò, F. Palma, G.M. Anelli, C. Novielli, E. Parejo Laudicina, V.M. Savasi, L. Barberini, A. Dessì, R. Pintu, V. Fano, A. Noto, I. Cetin

Subject Terms: INTRODUCTION: The placenta plays a pivotal role as it represents the connection between mother and foetus. It acts as a carrier (nutrients and oxygen, waste products and carbonic anhydride) as well as a barrier against infection. Pregnancy related metabolomics has examined different biofluids in order to reach deeper insights into pregnancy pathologies [1]. Amniotic fluid, urine and plasma were the most studied, while placenta tissue has been the subject of few studies [2]. In this report, a method to analyse the placental metabolome through the GC-MS (Gas Chromatography-Mass Spectrometry) technique is described. MATERIALS AND METHODS: Placenta tissues were collected after elective caesarean section from obese and normal weight mothers, snap frozen and stored at -80°C until analysis. Each sample was homogenized with a mixture of solvents (chloroform, methanol, water), centrifuged, and the obtained phases were separated and processed following two different protocols. The hydrophilic phase was vacuum dried and treated with two derivatizing reagents, methoxyamine hydrochloride and MSTFA (N-Methyl-N-[trimethylsilyl] trifluoroacetamide), to give a mixture of trimethylsilylated metabolites. The lipophilic phase was dried and treated with boron trifluoride in methanol to obtain a mixture of FAME (Fatty Acid Methyl Esters). The two phases were separately analysed on a GC-MS platform, applying different instrumental conditions. The chromatograms obtained were analysed with the free software AMDIS (Automated Mass Spectral Deconvolution and Identification System) using a lab-made library comprising 200 metabolites. RESULTS: The analysis of the placenta tissue allowed to identify essential metabolites. In the hydrophilic fraction, 78 metabolites were detected: they mainly belong to the classes of amino acids (18), carboxylic acids (13), sugars and sugar-related compounds (11), phosphorylated derivatives (9). The lipophilic fraction analysis allowed to define the fatty acid profile of the placenta extract: the saturated fatty acids palmitic and stearic, the monounsaturated oleic, and the polyunsaturated (PUFA) linoleic, arachidonic, 8,11,14-eicosatrienoic and 4,7,10,13,16,19-docosahexaenoic acid (DHA) were the most representative. Statistical analysis of the data matrices derived from the hydrophilic and lipophilic fraction analysis were performed in MetaboAnalyst 3.0 [3], to investigate the possibility of identifying characteristic metabolic differences between the different phenotypes. Placentae from obese mothers were compared with those from normal weight mothers to get new insights into the correlation between the metabolite content and metabolic status. CONCLUSIONS: The reported analysis method allowed to examine different metabolites in placenta samples, divided into two phases, hydrophilic and lipophilic. All metabolites were analysed through the same GC-MS platform to provide interesting informations about the placenta tissue composition. REFERENCES [1] Fanos V, Atzori L, Makarenko K, Melis GB, Ferrazzi E. Metabolomics application in maternal-fetal medicine. Biomed Res Int. 2013, 2013: 720514. [2] Dunn WB, Brown M

Relation: International Workshop on Neonatology; volume:6; issue:2; firstpage:81; lastpage:82; numberofpages:2; journal:JOURNAL OF PEDIATRIC AND NEONATAL INDIVIDUALIZED MEDICINE; https://hdl.handle.net/2434/545220

Availability: https://hdl.handle.net/2434/545220

GC–MS method for the determination of paraben preservatives in the human breast cancerous tissue

Authors: Shanmugam, Govindaraj ^a, Ramaswamy, Babu Rajendran ^{a, ⁎}, Radhakrishnan, Vijayalakshimi ^a, Tao, Hiroaki ^b

Source: In Microchemical Journal 2010 96(2):391-396

Analysis of sibutramine metabolites as N-trifluoroacetamide and O-trimethylsilyl derivatives by gas chromatography–mass spectrometry in urine

Authors: Sardela, V.F., Motta, M.T.R., Padilha, M.C., Pereira, H.M.G., Neto, F.R. Aquino ^⁎

Source: In Journal of Chromatography B 2009 877(27):3003-3011

Improvement in hydrophobicity of Ti-MCM-41 using a new silylation agent MSTFA

Authors: Bu, Jie, Rhee, Hyun-Ku

Source: Catalysis Letters. March 2000 65(1-3):141-145

Analysis of psilocybin and psilocin in Psilocybe subcubensis GUZMÁN by ion mobility spectrometry and gas chromatography–mass spectrometry

Authors: Keller, Thomas ^{a, *}, Schneider, Andrea ^a, Regenscheit, Priska ^b, Richard Dirnhofer ^b, Rücker, Thomas ^c, Jaspers, Jürgen ^d, Kisser, Wolfgang ^a

Source: In Forensic Science International 1999 99(2):93-105

Determination of Impurities in Bioproduced Succinic Acid

Authors: Rousová, J, Ondrušová, K, Karlová, P, Kubatova, Alena

Source: Chemistry Faculty Publications

Subject Terms: GC-MS, acids, Saccharides, BSTFA, MSTFA, Succinic acid, Bio-based succinic acid

File Description: application/pdf

Availability: https://commons.und.edu/chem-fac/7
https://doi.org/10.4172/2157-7064.1000264
https://commons.und.edu/context/chem-fac/article/1006/viewcontent/JChromSepT_2015_Succinic.pdf

Towards gas chromatography - mass spectrometry based metabolomics for the study of fusarium and wheat ; Entwicklung und Anwendung Gaschromatographie-Massenspektrometrie basierender Metabolomik-Methoden zur Untersuchung von Fusarium und Weizen

Authors: Schöfbeck, Denise

Subject Terms: Weizen, Ährenkrankheit, Fusarium, Mykotoxin, GC-MS, Metabolom, Fusarium Head Blight, Metabolic Profiling, HS SPME GC-MS, Targetanalytik, MSTFA, target analysis

Subject Geographic: BOK:H97000:H97300

File Description: 173 S.; text/html; Ill., graph. Darst.

Relation: vignette : https://epub.boku.ac.at/titlepage/urn/urn:nbn:at:at-ubbw:1-19026/128; local:990107774840203331; system:AC10774461

Availability: https://resolver.obvsg.at/urn:nbn:at:at-ubbw:1-19026

Bestimmung von 17alpha-Ethinylestradiol mittels GC/MS/MS nach SPE-Extraktion an EMPORE disks

Authors: Böhmer, W., Kurzawa, B.

Subject Terms: Ethinylestradiol, SPE-Extraktion, EMPORE disk, Silylierung, MSTFA, GC/MS/MS

Time: 570, 610, 620, 660, 616

Relation: GIT. Labor-Fachzeitschrift; https://publica.fraunhofer.de/handle/publica/199291

Availability: https://publica.fraunhofer.de/handle/publica/199291

Determination of 17alpha-ethinylestradiol using GC/MS/MS ; Bestimmung von 17alpha-Ethinylestradiol mittels GC/MS/MS

Authors: Böhmer, W., Kurzawa, B.

Subject Terms: 17alpha-Ethinylestradiol, SPE, EMPORE disk, silylation, MSTFA, GC/MS/MS

Time: 570, 610, 620, 660

Relation: GIT laboratory journal; https://publica.fraunhofer.de/handle/publica/199488

Availability: https://publica.fraunhofer.de/handle/publica/199488

Towards gas chromatography - mass spectrometry based metabolomics for the study of fusarium and wheat

Authors: Schöfbeck, Denise

Subject Terms: Fusarium Head Blight, MSTFA, Fusarium, Targetanalytik, Ährenkrankheit, metabolic profiling, target analysis, Mykotoxin, GC-MS, Weizen, HS SPME GC-MS, Metabolic Profiling, Metabolom

File Description: text/html; Ill., graph. Darst.

Access URL: https://resolver.obvsg.at/urn:nbn:at:at-ubbw:1-19026