A methodology for RNAi gene knockdown in the marine annelid Streblospio benedicti by delivering dsRNA.

Συγγραφείς: Aguilar-Camacho JM; Department of Biological Sciences, North Carolina State University, Raleigh, NC 27607, USA., Zakas C; Department of Biological Sciences, North Carolina State University, Raleigh, NC 27607, USA.

Πηγή: Development (Cambridge, England) [Development] 2026 Aug 01; Vol. 153 (15). Date of Electronic Publication: 2026 Aug 06.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Company Of Biologists Limited Country of Publication: England NLM ID: 8701744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9129 (Electronic) Linking ISSN: 09501991 NLM ISO Abbreviation: Development Subsets: MEDLINE

Ιατρικοί όροι (MeSH): RNA, Double-Stranded*/genetics , RNA, Double-Stranded*/administration & dosage , Gene Knockdown Techniques*/methods , Polychaeta*/genetics , RNA Interference*, Larva/genetics ; Actins/genetics ; Animals ; In Situ Hybridization ; Microinjections

Tissue-specific biochemical and molecular responses associated with red pigment-concentrating hormone gene knockdown in Litopenaeus vannamei.

Συγγραφείς: Feng L; Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, China., Luo J; Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, China., Zhou Y; Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, China., Lu W; Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, China., Huang Y; Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, China., Gu Z; Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, China., Jian J; Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, China., Cai S; Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, China., Yang S; Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, China. Electronic address: ysp20010@sina.com.

Πηγή: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2026 Oct-Dec; Vol. 286, pp. 111270. Date of Electronic Publication: 2026 Aug 17.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Pergamon Country of Publication: England NLM ID: 9516061 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1107 (Electronic) Linking ISSN: 10964959 NLM ISO Abbreviation: Comp Biochem Physiol B Biochem Mol Biol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Penaeidae*/genetics , Penaeidae*/metabolism , Pituitary Hormones*/genetics , Pituitary Hormones*/metabolism , Arthropod Proteins*/genetics , Arthropod Proteins*/metabolism , Gene Knockdown Techniques*, Hepatopancreas/metabolism ; Gills/metabolism ; Pyrrolidonecarboxylic Acid/analogs & derivatives ; Animals ; Organ Specificity ; Pigments, Biological ; Gene Expression Regulation ; RNA Interference ; Oligopeptides

Knockdown of METTL3 ameliorated myofibroblast activities in oral submucous fibrosis through upregulating DKK3.

Συγγραφείς: Peng J; Department of General Dentistry, Xindaxin Branch, Changsha Stomatological Hospital, Changsha, Hunan 410000, China. Electronic address: pengjing20100@163.com., Guo S; Dental Aesthetics Department, Changsha Stomatological Hospital, Changsha, Hunan 410000, China. Electronic address: guosheng11170@163.com., Zhang P; Department of General Dentistry, Xindaxin Branch, Changsha Stomatological Hospital, Changsha, Hunan 410000, China. Electronic address: zhangpeipei11060@163.com.

Πηγή: Tissue & cell [Tissue Cell] 2026 Oct; Vol. 102, pp. 103544. Date of Electronic Publication: 2026 Apr 20.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 0214745 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-3072 (Electronic) Linking ISSN: 00408166 NLM ISO Abbreviation: Tissue Cell Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Oral Submucous Fibrosis*/pathology , Oral Submucous Fibrosis*/genetics , Oral Submucous Fibrosis*/metabolism , Myofibroblasts*/metabolism , Myofibroblasts*/pathology , Methyltransferases*/metabolism , Methyltransferases*/genetics , Adaptor Proteins, Signal Transducing*/metabolism , Adaptor Proteins, Signal Transducing*/genetics , Intercellular Signaling Peptides and Proteins*/metabolism , Intercellular Signaling Peptides and Proteins*/genetics , Up-Regulation*/genetics , Gene Knockdown Techniques*, Cell Movement/genetics ; Humans

HOXA3 Knockdown Impairs hESC-Derived Thymic Epithelial-like Cell Differentiation and CXCL12-Associated Early T-Lineage Support.

Συγγραφείς: Shan H; Laboratory of Stem Cells and Translational Medicine, Center for Medical Research on Innovation and Translation, Institute of Clinical Medicine, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou 510006, China.; Laboratory of Stem Cells and Translational Medicine, Institute for Life Science, School of Medicine, South China University of Technology, Guangzhou 510006, China., Fu Y; Laboratory of Stem Cells and Translational Medicine, Center for Medical Research on Innovation and Translation, Institute of Clinical Medicine, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou 510006, China.; Laboratory of Stem Cells and Translational Medicine, Institute for Life Science, School of Medicine, South China University of Technology, Guangzhou 510006, China., Shi X; Laboratory of Stem Cells and Translational Medicine, Center for Medical Research on Innovation and Translation, Institute of Clinical Medicine, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou 510006, China.; Laboratory of Stem Cells and Translational Medicine, Institute for Life Science, School of Medicine, South China University of Technology, Guangzhou 510006, China., Chen S; Laboratory of Stem Cells and Translational Medicine, Center for Medical Research on Innovation and Translation, Institute of Clinical Medicine, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou 510006, China.; Laboratory of Stem Cells and Translational Medicine, Institute for Life Science, School of Medicine, South China University of Technology, Guangzhou 510006, China., Duan Y; Laboratory of Stem Cells and Translational Medicine, Center for Medical Research on Innovation and Translation, Institute of Clinical Medicine, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou 510006, China.; Laboratory of Stem Cells and Translational Medicine, Institute for Life Science, School of Medicine, South China University of Technology, Guangzhou 510006, China.; National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, China.; The Innovation Centre of Ministry of Education for Development and Diseases, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou 510006, China.

Πηγή: Cells [Cells] 2026 Sep 27; Vol. 15 (19). Date of Electronic Publication: 2026 Sep 27.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Cell Differentiation*/genetics , Epithelial Cells*/metabolism , Epithelial Cells*/cytology , Homeodomain Proteins*/metabolism , Homeodomain Proteins*/genetics , Thymus Gland*/cytology , Chemokine CXCL12*/metabolism , Chemokine CXCL12*/genetics , Human Embryonic Stem Cells*/metabolism , Human Embryonic Stem Cells*/cytology , T-Lymphocytes*/cytology , T-Lymphocytes*/metabolism , Gene Knockdown Techniques* , Cell Lineage*, Humans

Dnmt3b and Dnmt3l knockdown reduces blastocyst development in early mouse embryos.

Συγγραφείς: Bozdemir N; Department of Histology and Embryology, Ankara Medipol University School of Medicine, Altindag, 06050, Ankara, Turkey., Cinar O; Department of Histology and Embryology, Ankara University School of Medicine, Altindag, 06080, Ankara, Turkey., Oznacar T; Department of Biostatistics, Ankara Medipol University School of Medicine, Altindag, 06050, Ankara, Turkey., Ceylan AC; Department of Medical Genetics, Ankara Yıldırım Beyazit University School of Medicine, Cankaya, 06800, Ankara, Turkey., Cinar FU; Department of Histology and Embryology, Ankara Medipol University School of Medicine, Altindag, 06050, Ankara, Turkey. fatma.uysal@ankaramedipol.edu.tr.

Πηγή: Histochemistry and cell biology [Histochem Cell Biol] 2026 Sep 16; Vol. 164 (1). Date of Electronic Publication: 2026 Sep 16.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Springer Country of Publication: Germany NLM ID: 9506663 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-119X (Electronic) Linking ISSN: 09486143 NLM ISO Abbreviation: Histochem Cell Biol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): DNA (Cytosine-5-)-Methyltransferases*/genetics , DNA (Cytosine-5-)-Methyltransferases*/deficiency , DNA (Cytosine-5-)-Methyltransferases*/metabolism , Blastocyst*/metabolism , Blastocyst*/cytology , Embryonic Development*/genetics , Gene Knockdown Techniques*, RNA, Small Interfering/genetics ; Animals ; DNA Methyltransferase 3B ; Mice ; Female ; DNA Methylation

Sirtuin five knockdown induced the ferroptosis of hepatic stellate cells in hepatic fibrosis progression through de-succinylation modification of GPX4.

Συγγραφείς: Zhou J; College of Basic Medicine, Guiyang Healthcare Vocational University, Guiyang, Guizhou, China. Electronic address: zj18984813912@126.com., Zhang J; School of Basic Medicine, Guizhou Medical University, Guiyang, Guizhou, China., Zheng S; College of Pharmacy, Guiyang Healthcare Vocational University, Guiyang, Guizhou, China., Zhu J; College of Pharmacy, Guiyang Healthcare Vocational University, Guiyang, Guizhou, China., Wang K; College of Basic Medicine, Guiyang Healthcare Vocational University, Guiyang, Guizhou, China., Guo X; College of Basic Medicine, Guiyang Healthcare Vocational University, Guiyang, Guizhou, China.

Πηγή: The Journal of biological chemistry [J Biol Chem] 2026 Sep; Vol. 302 (9), pp. 113366. Date of Electronic Publication: 2026 Jul 24.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Ferroptosis*/genetics , Liver Cirrhosis*/metabolism , Liver Cirrhosis*/pathology , Liver Cirrhosis*/chemically induced , Sirtuins*/metabolism , Sirtuins*/genetics , Sirtuins*/deficiency , Hepatic Stellate Cells*/metabolism , Hepatic Stellate Cells*/pathology , Phospholipid Hydroperoxide Glutathione Peroxidase*/metabolism , Gene Knockdown Techniques*, Reactive Oxygen Species/metabolism ; Transforming Growth Factor beta1/pharmacology ; Animals ; Mice ; Humans ; Male ; Cell Line ; Mice, Inbred C57BL ; Disease Progression

MBD3 Knockdown Promotes the Differentiation of Bovine iPSCs into Primordial Germ Cell-like Cells Associated with Altered Bivalent Modification of TGF-β-Related Genes.

Συγγραφείς: Yuan W; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China.; College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China., Wang J; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Li W; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Luo Y; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Liu Y; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Long S; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Wang Y; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Liu F; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Geng X; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Yue Y; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China., Li X; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China.

Πηγή: Cells [Cells] 2026 Aug 30; Vol. 15 (17). Date of Electronic Publication: 2026 Aug 30.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Cell Differentiation*/genetics , Transforming Growth Factor beta*/metabolism , Transforming Growth Factor beta*/genetics , Germ Cells*/cytology , Germ Cells*/metabolism , DNA-Binding Proteins*/metabolism , DNA-Binding Proteins*/genetics , Induced Pluripotent Stem Cells*/metabolism , Induced Pluripotent Stem Cells*/cytology , Transcription Factors*/metabolism , Transcription Factors*/genetics , Gene Knockdown Techniques*, Animals ; Cattle ; Signal Transduction

A targeted mutational strategy aiding in generating antisense RNA to knockdown the Ehrlichia chaffeensis P28-outer membrane protein 19 expression.

Συγγραφείς: Tong X; Center of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA., Ferm D; Department of Pathobiology and Integrative Biomedical Sciences, Bond Life Sciences Center, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USA., Liu H; Center of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA.; Department of Pathobiology and Integrative Biomedical Sciences, Bond Life Sciences Center, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USA., Wang Y; Center of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA., Kondethimmanahalli C; Center of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA., Ganta RR; Center of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA.; Department of Pathobiology and Integrative Biomedical Sciences, Bond Life Sciences Center, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USA.

Πηγή: Journal of bacteriology [J Bacteriol] 2026 Aug 20; Vol. 208 (8), pp. e0033426. Date of Electronic Publication: 2026 Aug 04.

Τύπος έκδοσης: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.

Στοιχεία περιοδικού: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 2985120R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5530 (Electronic) Linking ISSN: 00219193 NLM ISO Abbreviation: J Bacteriol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Ehrlichia chaffeensis*/genetics , RNA, Antisense*/genetics , Gene Knockdown Techniques*/methods , Bacterial Outer Membrane Proteins*/genetics, Animals ; Mutation ; Mutagenesis ; Mice ; Gene Expression Regulation, Bacterial

Correlative Effects of PARP12 mRNA Knockdown on Pro-inflammatory Gene Expression and Alternative Splicing in BV2 Microglial Cells.

Συγγραφείς: Hu B; Hubei University of Chinese Medicine, Wuhan, 430065, People's Republic of China.; Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, 430074, People's Republic of China.; Hubei Shizhen Laboratory, Wuhan, 430074, People's Republic of China.; Department of Orthopedics, Affiliated Hospital of Hubei University of Chinese Medicine, Wuhan, 430074, People's Republic of China., Zhou W; Hubei University of Chinese Medicine, Wuhan, 430065, People's Republic of China.; Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, 430074, People's Republic of China.; Hubei Shizhen Laboratory, Wuhan, 430074, People's Republic of China.; Department of Orthopedics, Affiliated Hospital of Hubei University of Chinese Medicine, Wuhan, 430074, People's Republic of China., Chen H; Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, 430074, People's Republic of China. ch1222@126.com.; Hubei Shizhen Laboratory, Wuhan, 430074, People's Republic of China. ch1222@126.com.; Department of Orthopedics, Affiliated Hospital of Hubei University of Chinese Medicine, Wuhan, 430074, People's Republic of China. ch1222@126.com., Li F; Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, 430074, People's Republic of China. 690683196@qq.com.; Hubei Shizhen Laboratory, Wuhan, 430074, People's Republic of China. 690683196@qq.com.; Department of Orthopedics, Affiliated Hospital of Hubei University of Chinese Medicine, Wuhan, 430074, People's Republic of China. 690683196@qq.com.

Πηγή: Molecular neurobiology [Mol Neurobiol] 2026 Aug 15; Vol. 63 (1). Date of Electronic Publication: 2026 Aug 15.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Humana Press Country of Publication: United States NLM ID: 8900963 Publication Model: Electronic Cited Medium: Internet ISSN: 1559-1182 (Electronic) Linking ISSN: 08937648 NLM ISO Abbreviation: Mol Neurobiol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Microglia*/metabolism , Microglia*/pathology , Alternative Splicing*/genetics , RNA, Messenger*/genetics , RNA, Messenger*/metabolism , Inflammation*/genetics , Inflammation*/pathology , Poly(ADP-ribose) Polymerases*/genetics , Poly(ADP-ribose) Polymerases*/metabolism , Inflammation Mediators*/metabolism , Gene Knockdown Techniques* , Gene Expression Regulation*, Transcriptome/genetics ; Animals ; Mice ; Cell Line

UBA1 knockdown dysregulates UBA1-sensitive proteins and impairs muscle function in models of spinal muscular atrophy X-linked 2.

Συγγραφείς: Curley M; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA., Graca FA; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA., Stephan A; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA., Wang W; Center for Proteomics and Metabolomics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA., Pagala VR; Center for Proteomics and Metabolomics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA., Wang YD; Department of Cellular and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA., High AA; Center for Proteomics and Metabolomics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA., Demontis F; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.

Πηγή: Disease models & mechanisms [Dis Model Mech] 2026 Aug 01; Vol. 19 (8). Date of Electronic Publication: 2026 Aug 26.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Company of Biologists Ltd Country of Publication: England NLM ID: 101483332 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1754-8411 (Electronic) Linking ISSN: 17548403 NLM ISO Abbreviation: Dis Model Mech Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Ubiquitin-Activating Enzymes*/metabolism , Ubiquitin-Activating Enzymes*/genetics , Muscular Atrophy, Spinal*/physiopathology , Muscular Atrophy, Spinal*/genetics , Muscular Atrophy, Spinal*/metabolism , Muscular Atrophy, Spinal*/pathology , Muscle, Skeletal*/physiopathology , Muscle, Skeletal*/pathology , Gene Knockdown Techniques*, Drosophila melanogaster/metabolism ; Drosophila melanogaster/genetics ; Animals ; Ubiquitination ; Disease Models, Animal ; RNA Interference ; Humans

FAM72A Knockdown Drives Cell Mitophagy and Pyroptosis in Ovarian Cancer Via the PINK1/Parkin Pathway.

Συγγραφείς: Tan S; Department of Gynecology, Affiliated Hengyang Hospital of Hunan Normal University & Hengyang Central Hospital, No. 31 Guanghui Road, Zhengxiang District, Hengyang City, Hunan, 421000, China., Wang L; Department of Gynecology, Tongren People's Hospital, Tongren, Guizhou, 5543000, China., Xu Z; Department of Gynecology, Affiliated Hengyang Hospital of Hunan Normal University & Hengyang Central Hospital, No. 31 Guanghui Road, Zhengxiang District, Hengyang City, Hunan, 421000, China., Tang R; Department of Gynecology, Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan Province, 421000, China., Xie W; Department of Gynecology, Affiliated Hengyang Hospital of Hunan Normal University & Hengyang Central Hospital, No. 31 Guanghui Road, Zhengxiang District, Hengyang City, Hunan, 421000, China. f_199901@163.com.

Πηγή: Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2026 Aug; Vol. 198 (8), pp. 6193-6214. Date of Electronic Publication: 2026 May 20.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Humana Press Country of Publication: United States NLM ID: 8208561 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0291 (Electronic) Linking ISSN: 02732289 NLM ISO Abbreviation: Appl Biochem Biotechnol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Pyroptosis*/genetics , Mitophagy*/genetics , Protein Kinases*/metabolism , Protein Kinases*/genetics , Ovarian Neoplasms*/metabolism , Ovarian Neoplasms*/pathology , Ovarian Neoplasms*/genetics , Ubiquitin-Protein Ligases*/metabolism , Ubiquitin-Protein Ligases*/genetics , Neoplasm Proteins*/metabolism , Neoplasm Proteins*/genetics , Gene Knockdown Techniques* , Signal Transduction*, Mitochondria/metabolism ; Female ; Humans ; PTEN-Induced Putative Kinase ; Animals ; Cell Line, Tumor ; Mice ; Mice, Nude

PDK4 knockdown suppresses osteoclast differentiation in osteoporosis through glycolytic flux suppression and AMPK activation.

Συγγραφείς: Huang Y; Department of Nephrology, Shanghai Eighth People's Hospital, No. 8 Caobao Road, Xuhui District, Shanghai, 200235, China., Zhou R; Department of General Practice, Zhongshan Hospital, Fudan University, No. 180 Fenglin Road, Xuhui District, Shanghai, 200032, China., Liang R; Department of Nephrology, Shanghai Eighth People's Hospital, No. 8 Caobao Road, Xuhui District, Shanghai, 200235, China., Shen J; Department of Geriatrics, Zhongshan Hospital, Fudan University, No. 180 Fenglin Road, Xuhui District, Shanghai, 200032, China. Electronic address: jamesjp801013@163.com.

Πηγή: Bone [Bone] 2026 Aug; Vol. 209, pp. 117911. Date of Electronic Publication: 2026 Apr 27.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Elsevier Science Country of Publication: United States NLM ID: 8504048 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2763 (Electronic) Linking ISSN: 18732763 NLM ISO Abbreviation: Bone Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Glycolysis*/genetics , Glycolysis*/drug effects , Osteoclasts*/metabolism , Osteoclasts*/pathology , Cell Differentiation*/genetics , Cell Differentiation*/drug effects , AMP-Activated Protein Kinases*/metabolism , Osteoporosis*/pathology , Osteoporosis*/metabolism , Osteoporosis*/genetics , Osteoporosis*/enzymology , Pyruvate Dehydrogenase Acetyl-Transferring Kinase*/metabolism , Pyruvate Dehydrogenase Acetyl-Transferring Kinase*/genetics , Gene Knockdown Techniques*, Animals ; Female ; Mice ; Rats ; RAW 264.7 Cells ; Rats, Sprague-Dawley ; Enzyme Activation

Conditional dCas12-Mediated Gene Knockdown and Complementation in Chlamydia.

Συγγραφείς: Wang Y; Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers University, Piscataway, NJ, 08820, USA., Fan H; Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers University, Piscataway, NJ, 08820, USA. fanhu@rwjms.rutgers.edu.

Πηγή: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2027; Vol. 3073, pp. 243-258.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Gene Knockdown Techniques*/methods , Chlamydia trachomatis*/genetics , Genetic Complementation Test*/methods , CRISPR-Associated Proteins*/genetics , CRISPR-Associated Proteins*/metabolism , CRISPR-Cas Systems*, RNA, Guide, CRISPR-Cas Systems/genetics ; Humans

Inducible Protein Knockdown via Engineered Small RNAs in Chlamydia.

Συγγραφείς: Densi A; Department of Developmental and Cell Biology, University of California, Irvine, CA, USA., Tan M; Department of Microbiology and Molecular Genetics and Department of Medicine, University of California, Irvine, CA, USA., Sütterlin C; Department of Developmental and Cell Biology, University of California, Irvine, CA, USA. suetterc@uci.edu.

Πηγή: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2027; Vol. 3073, pp. 231-241.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Gene Knockdown Techniques*/methods , Chlamydia trachomatis*/genetics , Bacterial Proteins*/genetics , RNA, Small Untranslated*/genetics, Genetic Engineering/methods ; RNA, Bacterial/genetics ; RNA, Small Interfering/genetics ; 5' Untranslated Regions ; Humans

Comparison of knockdown approaches for the generation of stable cell populations expressing afucosylated antibodies.

Συγγραφείς: Tsachaki M; Drug Substance Development, IGI Therapeutics SA, La Chaux-de-Fonds, Switzerland., Samy A; Drug Substance Development, IGI Therapeutics SA, La Chaux-de-Fonds, Switzerland., Peltret M; Drug Substance Development, IGI Therapeutics SA, La Chaux-de-Fonds, Switzerland., Brunstein Laplace T; Drug Substance Development, IGI Therapeutics SA, La Chaux-de-Fonds, Switzerland., Vaxelaire-Hily E; Drug Substance Development, IGI Therapeutics SA, La Chaux-de-Fonds, Switzerland., Mosbaoui F; Drug Substance Development, IGI Therapeutics SA, La Chaux-de-Fonds, Switzerland., Galea A; Drug Substance Development, IGI Therapeutics SA, La Chaux-de-Fonds, Switzerland., Contarin F; Drug Substance Development, IGI Therapeutics SA, La Chaux-de-Fonds, Switzerland., Duarte L; Drug Substance Development, IGI Therapeutics SA, La Chaux-de-Fonds, Switzerland., Giovannini R; Process Development, IGI Therapeutics SA, La Chaux-de-Fonds, Switzerland., Bertschinger M; Drug Substance Development, IGI Therapeutics SA, La Chaux-de-Fonds, Switzerland.

Πηγή: MAbs [MAbs] 2026 Dec; Vol. 18 (1), pp. 2684393. Date of Electronic Publication: 2026 Jun 12.

Τύπος έκδοσης: Journal Article; Comparative Study

Στοιχεία περιοδικού: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101479829 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1942-0870 (Electronic) Linking ISSN: 19420862 NLM ISO Abbreviation: MAbs Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Fucosyltransferases*/genetics , Fucosyltransferases*/metabolism , Gene Knockdown Techniques*/methods , Antibodies, Monoclonal*/genetics , Antibodies, Monoclonal*/biosynthesis , Antibodies, Monoclonal*/metabolism , Fucose*/metabolism, RNA, Small Interfering/genetics ; MicroRNAs/genetics ; Recombinant Proteins/genetics ; Recombinant Proteins/biosynthesis ; Animals ; CHO Cells ; Cricetulus ; Cricetinae ; Glycosylation ; Humans

Design guide for synthetic small regulatory RNAs for high-efficiency gene knockdown in bacteria.

Συγγραφείς: Ren J; Department of Biomedical Engineering, Chung-Ang University, Seoul 06974, Republic of Korea., Kim Y; Department of Biomedical Engineering, Chung-Ang University, Seoul 06974, Republic of Korea., Nam HJ; Department of Biomedical Engineering, Chung-Ang University, Seoul 06974, Republic of Korea., Lee HM; Department of Biomedical Engineering, Chung-Ang University, Seoul 06974, Republic of Korea., Na D; Department of Biomedical Engineering, Chung-Ang University, Seoul 06974, Republic of Korea.

Πηγή: Journal of microbiology (Seoul, Korea) [J Microbiol] 2026 Jul; Vol. 64 (7), pp. e2603026. Date of Electronic Publication: 2026 Jul 06.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Microbiological Society Of Korea Country of Publication: Korea (South) NLM ID: 9703165 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1976-3794 (Electronic) Linking ISSN: 12258873 NLM ISO Abbreviation: J Microbiol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Gene Knockdown Techniques*/methods , Escherichia coli*/genetics , Escherichia coli*/metabolism , RNA, Small Untranslated*/genetics , RNA, Small Untranslated*/chemical synthesis , Bacteria*/genetics, Synthetic Biology/methods ; Metabolic Engineering/methods ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Messenger/chemistry ; Host Factor 1 Protein/genetics ; Host Factor 1 Protein/metabolism ; RNA, Bacterial/genetics ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Gene Expression Regulation, Bacterial

KPNA2 knockdown suppresses gastric cancer progression through modulation of the β-catenin/EMT signaling axis, inducing cell cycle arrest, apoptosis, and reduced metastatic capacity.

Συγγραφείς: Zhu H; Department of Gastrointestinal Surgery, Affiliated Nantong Clinical College of Nantong University, Nantong First People's Hospital, No. 666, Shengli Road, Nantong, 226001, Jiangsu, China., Niu T; Department of Gastrointestinal Surgery, Affiliated Nantong Clinical College of Nantong University, Nantong First People's Hospital, No. 666, Shengli Road, Nantong, 226001, Jiangsu, China., Feng S; Department of Gastrointestinal Surgery, Affiliated Nantong Clinical College of Nantong University, Nantong First People's Hospital, No. 666, Shengli Road, Nantong, 226001, Jiangsu, China., Shi C; Department of Pathology, Affiliated Nantong Clinical College of Nantong University, Nantong First People's Hospital, Nantong, 226001, Jiangsu, China., Tang C; Department of Gastrointestinal Surgery, Affiliated Nantong Clinical College of Nantong University, Nantong First People's Hospital, No. 666, Shengli Road, Nantong, 226001, Jiangsu, China. 15051273108@163.com., Wang F; Department of Gastrointestinal Surgery, Affiliated Nantong Clinical College of Nantong University, Nantong First People's Hospital, No. 666, Shengli Road, Nantong, 226001, Jiangsu, China. 15152890693@163.com.

Πηγή: Journal of molecular histology [J Mol Histol] 2026 Jul 06; Vol. 57 (4). Date of Electronic Publication: 2026 Jul 06.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Springer Netherlands Country of Publication: Netherlands NLM ID: 101193653 Publication Model: Electronic Cited Medium: Internet ISSN: 1567-2387 (Electronic) Linking ISSN: 15672379 NLM ISO Abbreviation: J Mol Histol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Stomach Neoplasms*/pathology , Stomach Neoplasms*/genetics , Stomach Neoplasms*/metabolism , Apoptosis*/genetics , alpha Karyopherins*/genetics , alpha Karyopherins*/metabolism , beta Catenin*/metabolism , Epithelial-Mesenchymal Transition*/genetics , Cell Cycle Checkpoints*/genetics , Signal Transduction* , Gene Knockdown Techniques*, Cell Proliferation/genetics ; Cell Movement/genetics ; Humans ; Cell Line, Tumor ; Animals ; Gene Expression Regulation, Neoplastic ; Disease Progression ; Mice ; Neoplasm Metastasis ; Female ; Mice, Nude

Knockdown of GBP5 Attenuates Abdominal Aortic Aneurysm Formation via the NF-κB Pathway.

Συγγραφείς: Wang Y; Department of Cardiac Surgery, Shandong Provincial Third Hospital, Shandong University, No. 11 Wuyingshan Middle Road, Tianqiao District, Jinan, 250031, Shandong, China., Wang J; Department of Cardiac Surgery, Shandong Provincial Third Hospital, Shandong University, No. 11 Wuyingshan Middle Road, Tianqiao District, Jinan, 250031, Shandong, China., Chang Z; Department of Cardiac Surgery, Shandong Provincial Third Hospital, Shandong University, No. 11 Wuyingshan Middle Road, Tianqiao District, Jinan, 250031, Shandong, China. 153119434@qq.com.

Πηγή: Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2026 Jul; Vol. 198 (7), pp. 5293-5308. Date of Electronic Publication: 2026 Apr 17.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Humana Press Country of Publication: United States NLM ID: 8208561 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0291 (Electronic) Linking ISSN: 02732289 NLM ISO Abbreviation: Appl Biochem Biotechnol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Aortic Aneurysm, Abdominal*/metabolism , Aortic Aneurysm, Abdominal*/pathology , Aortic Aneurysm, Abdominal*/genetics , Aortic Aneurysm, Abdominal*/chemically induced , NF-kappa B*/metabolism , GTP-Binding Proteins*/genetics , GTP-Binding Proteins*/metabolism , Signal Transduction* , Gene Knockdown Techniques*, Angiotensin II/pharmacology ; Myocytes, Smooth Muscle/metabolism ; Myocytes, Smooth Muscle/pathology ; Toll-Like Receptor 4/metabolism ; Muscle, Smooth, Vascular/metabolism ; Muscle, Smooth, Vascular/pathology ; Animals ; Mice ; RAW 264.7 Cells ; Male ; Mice, Inbred C57BL

Kbtbd13 knockdown restores muscle function in a clinically relevant mouse model of nemaline myopathy type 6.

Συγγραφείς: Galli RA; Department of Physiology, Amsterdam UMC, Location VUMC, 1081HV Amsterdam, Netherlands.; Musculoskeletal Health, Amsterdam Movement Sciences, 1081HV Amsterdam, Netherlands., Vonk LA; Department of Physiology, Amsterdam UMC, Location VUMC, 1081HV Amsterdam, Netherlands., Baelde RJ; Department of Physiology, Amsterdam UMC, Location VUMC, 1081HV Amsterdam, Netherlands., van den Heuvel A; Department of Human Genetics, Leiden University Medical Center, 2333ZC Leiden, Netherlands., Bogaards S; Department of Physiology, Amsterdam UMC, Location VUMC, 1081HV Amsterdam, Netherlands., Conijn S; Department of Physiology, Amsterdam UMC, Location VUMC, 1081HV Amsterdam, Netherlands., Siteur E; Department of Physiology, Amsterdam UMC, Location VUMC, 1081HV Amsterdam, Netherlands., Alekhina O; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, USA., Granzier H; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, USA., Irving T; BioCAT, Department of Biology, Illinois Institute of Technology, Chicago, IL 60616, USA.; Center for Synchrotron Radiation Research and Instrumentation, Illinois Institute of Technology, Chicago, IL 60616, USA., Ma W; BioCAT, Department of Biology, Illinois Institute of Technology, Chicago, IL 60616, USA.; Center for Synchrotron Radiation Research and Instrumentation, Illinois Institute of Technology, Chicago, IL 60616, USA.; Pritzker Institute of Biomedical Science and Engineering, Illinois Institute of Technology, Chicago, IL 60616, USA., Bouman K; Department of Neurology, Donders Institute for Brain, Cognition, and Behaviour, Radboud University Medical Center, 6525EN Nijmegen, Netherlands., van Engelen B; Department of Neurology, Donders Institute for Brain, Cognition, and Behaviour, Radboud University Medical Center, 6525EN Nijmegen, Netherlands., van der Maarel SM; Department of Human Genetics, Leiden University Medical Center, 2333ZC Leiden, Netherlands., Kirby TJ; Department of Physiology, Amsterdam UMC, Location VUMC, 1081HV Amsterdam, Netherlands.; Tissue Function & Regeneration, Amsterdam Movement Sciences, 1081HV Amsterdam, Netherlands.; Heart Failure & Arrhythmias, Amsterdam Cardiovascular Sciences, 1081HV Amsterdam, Netherlands.; Division of Cardiovascular Medicine, Department of Internal Medicine, University of Kentucky, Lexington, KY 40536, USA., Voermans N; Department of Neurology, Donders Institute for Brain, Cognition, and Behaviour, Radboud University Medical Center, 6525EN Nijmegen, Netherlands., de Winter J; Department of Physiology, Amsterdam UMC, Location VUMC, 1081HV Amsterdam, Netherlands.; Tissue Function & Regeneration, Amsterdam Movement Sciences, 1081HV Amsterdam, Netherlands., Ottenheijm CAC; Department of Physiology, Amsterdam UMC, Location VUMC, 1081HV Amsterdam, Netherlands.; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, USA.; Heart Failure & Arrhythmias, Amsterdam Cardiovascular Sciences, 1081HV Amsterdam, Netherlands.

Πηγή: Science translational medicine [Sci Transl Med] 2026 Jun 24; Vol. 18 (855), pp. eadx2116. Date of Electronic Publication: 2026 Jun 24.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101505086 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1946-6242 (Electronic) Linking ISSN: 19466234 NLM ISO Abbreviation: Sci Transl Med Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Myopathies, Nemaline*/physiopathology , Myopathies, Nemaline*/genetics , Myopathies, Nemaline*/pathology , Myopathies, Nemaline*/therapy , Muscle, Skeletal*/physiopathology , Muscle, Skeletal*/pathology , Muscle Proteins*/metabolism , Muscle Proteins*/genetics , Gene Knockdown Techniques*, Sarcomeres/metabolism ; Animals ; Disease Models, Animal ; Humans ; Muscle Contraction ; Mice

Protocol for gene knockdown in Trichoplax adhaerens using a silica nanoparticle-mediated RNA interference technique.

Συγγραφείς: Guo Q; Fang Zongxi Center for Marine EvoDevo, MoE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China., Li W; Fang Zongxi Center for Marine EvoDevo, MoE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China. Electronic address: wanqing_li@ouc.edu.cn., Zheng W; Key Laboratory of Marine Chemistry Theory and Technology, Ocean University of China, Ministry of Education, Qingdao 266100, China., Qiu M; Key Laboratory of Marine Chemistry Theory and Technology, Ocean University of China, Ministry of Education, Qingdao 266100, China., Zhao C; Fang Zongxi Center for Marine EvoDevo, MoE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China. Electronic address: chengtian_zhao@ouc.edu.cn.

Πηγή: STAR protocols [STAR Protoc] 2026 Jun 19; Vol. 7 (2), pp. 104484. Date of Electronic Publication: 2026 Apr 02.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Silicon Dioxide*/chemistry , Nanoparticles*/chemistry , Gene Knockdown Techniques*/methods , RNA Interference*