Cryo-Electron Microscopy-Based Epitope Mapping

Authors: Drulyte, Ieva, Hurdiss, Daniel L

Source: Methods in Molecular Biology ISBN: 9781071645901

Subject Terms: Epitopes, SDG 3 - Good Health and Well-being, Antigen-Antibody Complex/immunology, Cryoelectron Microscopy, Epitope Mapping/methods, Humans, Epitopes/immunology, Cryoelectron Microscopy/methods, Antigen-Antibody Complex, Epitope Mapping

Access URL: https://pubmed.ncbi.nlm.nih.gov/40593414

Simultaneous polyclonal antibody sequencing and epitope mapping by cryo electron microscopy and mass spectrometry

Authors: Schulte, Douwe, Šiborová, Marta, Käll, Lukas, Snijder, Joost

Source: eLife
eLife, Vol 14 (2025)

Subject Terms: Proteomics, QH301-705.5, Science, Cryoelectron Microscopy/methods, Tandem Mass Spectrometry/methods, Antibodies, proteomics, Immunology and Inflammation, Tandem Mass Spectrometry, Sequence Analysis, Protein, antibody, Humans, Biology (General), Chromatography, Liquid, epitope, Cryoelectron Microscopy, repertoire, cryoEM, Epitope Mapping/methods, Medicine, Proteomics/methods, Protein/methods, Antibodies/chemistry, Sequence Analysis, Epitope Mapping, Chromatography, Liquid

Access URL: https://pubmed.ncbi.nlm.nih.gov/40266252
https://doaj.org/article/dacc2b379ef0412fb4311005f9cc6368

Epitope Mapping with Sidewinder : An XL-MS and Structural Modeling Approach

Authors: Ströbaek, Joel, Tang, Di, Gueto-Tettay, Carlos, Gomez Toledo, Alejandro, Olofsson, Berit, Hartman, Erik, Heusel, Moritz, Malmström, Johan, Malmström, Lars

Source: International Journal of Molecular Sciences; 26(4), pp 1-17 (2025) ; ISSN: 1422-0067

Subject Terms: Bioinformatics (Computational Biology), Epitope Mapping/methods, Streptococcus pyogenes/immunology, Bacterial Proteins/chemistry, Molecular Docking Simulation, Antibodies, Monoclonal/chemistry, Mass Spectrometry/methods, Epitopes/immunology, Streptolysins/chemistry, Bacterial/immunology

Relation: http://dx.doi.org/10.3390/ijms26041488; pmid:40003954; scopus:85218901966

Availability: https://lup.lub.lu.se/record/18960a88-0ea4-4ce7-a6c1-34132a89496d
https://doi.org/10.3390/ijms26041488

The XcpV/GspI Pseudopilin Has a Central Role in the Assembly of a Quaternary Complex within the T2SS Pseudopilus

Authors: Douzi, Badreddine, Alphonse, Sébastien, Darbon, Herve, Filloux, Alain, Cambillau, Christian, Bernard, Cedric, Tegoni, Mariella, Voulhoux, Romé

Contributors: ProdInra, Migration

Source: Journal of Biological Chemistry. 284:34580-34589

Subject Terms: 0301 basic medicine, Protein Structure, 0303 health sciences, Protein Isoforms/*chemistry/genetics/*metabolism, Protein Conformation, [SDV]Life Sciences [q-bio], Pseudomonas aeruginosa/cytology/genetics/*metabolism, Surface Plasmon Resonance, Protein Structure, Tertiary, [SDV] Life Sciences [q-bio], 03 medical and health sciences, Bacterial Proteins, Multiprotein Complexes/metabolism, Multiprotein Complexes, Pseudomonas aeruginosa, Epitope Mapping/methods, Protein Isoforms, Protein Multimerization, Tertiary, Bacterial Proteins/*chemistry/genetics/*metabolism, Epitope Mapping

File Description: application/pdf

Access URL: http://www.jbc.org/content/284/50/34580.full.pdf
https://pubmed.ncbi.nlm.nih.gov/19828448
https://hal.inrae.fr/hal-02824819v1
https://hal.inrae.fr/hal-02824819v1/document
https://hal.inrae.fr/hal-02666049v1
https://doi.org/10.1074/jbc.m109.042366

Epitope Mapping and Fine Specificity of Human T and B Cell Responses for Novel Candidate Blood-Stage Malaria Vaccine P27A

Authors: Kristina M. Geiger, Daniel Guignard, Che Yang, Jean-Pierre Bikorimana, Bruno E. Correia, Sophie Houard, Catherine Mkindi, Claudia A. Daubenberger, François Spertini, Giampietro Corradin, Régine Audran

Source: Front Immunol
Frontiers in Immunology, Vol 11 (2020)
Frontiers in immunology.-2020
Frontiers in immunology, vol. 11, pp. 412

Subject Terms: Adult, 0301 basic medicine, Adjuvants, Immunologic, Antibodies, Protozoan/metabolism, Antibody Affinity, Antigens, Protozoan/genetics, Antigens, Protozoan/immunology, Epitope Mapping/methods, Epitopes, B-Lymphocyte/genetics, Epitopes, B-Lymphocyte/immunology, Epitopes, T-Lymphocyte/genetics, Epitopes, T-Lymphocyte/immunology, Humans, Life Cycle Stages, Lymphocyte Activation, Malaria Vaccines/immunology, Malaria, Falciparum/immunology, Peptides/genetics, Peptides/immunology, Plasmodium falciparum, Protozoan Proteins/genetics, Protozoan Proteins/immunology, Switzerland, Tanzania, Vaccination, P27A, adjuvant, clinical trial, immune response, malaria, populations, vaccine, Immunology, Protozoan Proteins, Antibodies, Protozoan, Epitopes, T-Lymphocyte, Antigens, Protozoan, 03 medical and health sciences, Malaria Vaccines, Malaria, Falciparum, RC581-607, 3. Good health, Epitopes, B-Lymphocyte, Immunologic diseases. Allergy, Peptides, Epitope Mapping

File Description: application/pdf

Access URL: https://www.frontiersin.org/articles/10.3389/fimmu.2020.00412/pdf
https://pubmed.ncbi.nlm.nih.gov/32210975
https://doaj.org/article/8aa2e3adbc2a4cbca7e1e48344279cbc
https://sonar.ch/global/documents/172983
https://serval.unil.ch/resource/serval:BIB_941F355259B5.P001/REF.pdf
https://serval.unil.ch/notice/serval:BIB_941F355259B5
http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_941F355259B52

Automatic Generation of Validated Specific Epitope Sets

Authors: Sebastian Carrasco Pro, John Sidney, Sinu Paul, Cecilia S. Lindestam Arlehamn, Daniela Weiskopf, Bjoern Peters, Alessandro Sette

Source: J Immunol Res
Journal of Immunology Research, Vol 2015 (2015)

Subject Terms: 0301 basic medicine, Herpesvirus 4, Human, Databases, Factual, Epitopes, T-Lymphocyte/metabolism, T-Lymphocytes, Epitopes, T-Lymphocyte, Lymphocyte Activation, Prediction of Peptide-MHC Binding Affinity, Computational biology, Context (archaeology), 0302 clinical medicine, Precision Medicine, Mycobacteriaceae, Cells, Cultured, Immunology and Microbiology, Dengue Virus/immunology, Life Sciences, 3. Good health, Antigen, Mycobacteriaceae/immunology, Immune Responses, Epitope, Immunotherapy, Research Article, Herpesvirus 4, Human/immunology, T-Cell Epitopes, Immunobiology of Dendritic Cells, Immunology, Immunologic Tests, Viral Proteins, 03 medical and health sciences, Biochemistry, Genetics and Molecular Biology, Virology, Humans, Molecular Biology, Biology, Electronic Data Processing, FOS: Clinical medicine, T cell, Paleontology, T-Lymphocytes/immunology, RC581-607, Dengue Virus, Computer science, Epitope Mapping/methods, Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome, Viral Proteins/immunology, Epitope Prediction, Immunologic diseases. Allergy, B-Cell Epitopes, Epitope Mapping, Software

File Description: text/xhtml

Access URL: http://downloads.hindawi.com/journals/jir/2015/763461.pdf
https://pubmed.ncbi.nlm.nih.gov/26568965
https://doaj.org/article/f64e530d503d4d40bbf6209b232b1c7c
https://hdl.handle.net/20.500.12866/5352
https://doi.org/10.1155/2015/763461

Molecular and Therapeutic Characterization of Anti-ectodysplasin A Receptor (EDAR) Agonist Monoclonal Antibodies

Authors: Kowalczyk Christine, Dunkel Nathalie, Willen Laure, Casal Margret L, Mauldin Elizabeth A, Gaide Olivier, Tardivel Aubry, Badic Giovanna, Etter Anne-Lise, Favre Manuel, Jefferson Douglas M, Headon Denis J, Demotz Stéphane, Schneider Pascal

Source: The Journal of biological chemistry.-2011
Journal of Biological Chemistry, vol. 286, no. 35, pp. 30769-30779
Kowalczyk, C, Dunkel, N, Willen, L, Casal, M L, Mauldin, E A, Gaide, O, Tardivel, A, Badic, G, Etter, A L, Favre, M, Jefferson, D M, Headon, D J, Demotz, S & Schneider, P 2011, 'Molecular and therapeutic characterization of anti-Ectodysplasin A receptor (EDAR) agonist monoclonal antibodies', Journal of Biological Chemistry, vol. 286, no. 35, pp. 30769-30779. https://doi.org/10.1074/jbc.M111.267997
J Biol Chem
The Journal of biological chemistry

Subject Terms: Animals, Antibodies, Monoclonal/chemistry, Cell Separation, Chickens, Dogs, Enzyme-Linked Immunosorbent Assay/methods, Epitope Mapping/methods, Flow Cytometry, Humans, Ligands, Mice, Molecular Sequence Data, Mutation, Phenotype, Plasmids/metabolism, Rats, Receptors, Ectodysplasin/chemistry, Receptors, Ectodysplasin/immunology, Surface Plasmon Resonance, Tooth/embryology, Tumor Necrosis Factor-alpha/metabolism, 0301 basic medicine, 0303 health sciences, Receptors, Ectodysplasin, Tumor Necrosis Factor-alpha, Antibodies, Monoclonal, Enzyme-Linked Immunosorbent Assay, 3. Good health, 03 medical and health sciences, Tooth, Epitope Mapping, Plasmids

File Description: application/pdf

Access URL: http://www.jbc.org/content/286/35/30769.full.pdf
https://pubmed.ncbi.nlm.nih.gov/21730053
https://sonar.ch/global/documents/106025
https://serval.unil.ch/notice/serval:BIB_9E190030D736
http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_9E190030D7360
https://serval.unil.ch/resource/serval:BIB_9E190030D736.P001/REF.pdf

Zebrafish to humans: evolution of the alpha3-chain of type IV collagen and emergence of the autoimmune epitopes associated with Goodpasture syndrome.

Authors: MacDonald, Brian A, Author, Sund, Malin, Author, Grant, Marianne A, Author, Pfaff, Kathleen L, Author, Holthaus, Kathryn, Author, Zon, Leonard I, Author, Kalluri, Raghu, Author

Contributors: MacDonald, Brian A, Author, Sund, Malin, Author, Grant, Marianne A, Author, Pfaff, Kathleen L, Author, Holthaus, Kathryn, Author, Zon, Leonard I, Author, Kalluri, Raghu, Author

Source: Blood. 107(5):1908-1915

Subject Terms: Animals, Anti-Glomerular Basement Membrane Disease/genetics/*immunology, Antibody Specificity/immunology, Autoantibodies/*immunology, Autoantigens/genetics/*immunology, Collagen Type IV/genetics/*immunology, Epitope Mapping/methods, Epitopes/genetics/*immunology, Evolution, Molecular, Humans, Mice, Sequence Homology, Amino Acid, Species Specificity, Structural Homology, Protein, Zebrafish/genetics/immunology

File Description: print

Access URL: https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-6057

Evolutionary and immunological implications of contemporary HIV-1 variation

Authors: Korber, Bette T, Gaschen, Brian, Yusim, Karina, Thakallapally, R, Kesmir, Can, Detours, Vincent

Source: British Medical Bulletin, 58

Subject Terms: T-Lymphocytes, Cytotoxic -- drug effects, Evolution, T-Lymphocytes, Genetic Variation -- immunology, HIV-1 -- pathogenicity, Cross Reactions, Global Health, Evolution, Molecular, Epitope Mapping -- methods, Epitopes, 03 medical and health sciences, 0302 clinical medicine, HIV-1 -- genetics, HIV-1 -- immunology, Animals, Humans, Mutation -- genetics, Antigenic Variation -- genetics, Cross Reactions -- immunology, Genetic Variation -- genetics, Epitopes -- genetics, Phylogeny, Haemophilus Vaccines, AIDS Vaccines, Molecular, Genetic Variation, Sciences bio-médicales et agricoles, Antigenic Variation, 3. Good health, Cytotoxic -- drug effects, Influenza A virus -- genetics, AIDS Vaccines -- immunology, Influenza A virus, Drug Design, World Health, Mutation, HIV-1, Haemophilus Vaccines -- pharmacology, Epitope Mapping, T-Lymphocytes, Cytotoxic

File Description: 2 full-text file(s): application/pdf; application/pdf

Access URL: https://academic.oup.com/bmb/article-pdf/58/1/19/25151348/19.pdf
https://pubmed.ncbi.nlm.nih.gov/11714622
https://dipot.ulb.ac.be/dspace/bitstream/2013/50687/6/2e05663e-0628-4c47-980c-b45c1d051a3e.txt
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/50687
https://dipot.ulb.ac.be/dspace/bitstream/2013/50687/3/doi_25749.pdf

Mapping thyroid peroxidase epitopes using recombinant protein fragments

Authors: TONACCHERA, MASSIMO, CETANI F, COSTAGLIOLA S, ALCALDE L, UIBO R, VASSART G, LUDGATE M.

Source: European journal of endocrinology, 132 (1

Subject Terms: Adult, Male, 0301 basic medicine, Adolescent, Molecular Probes -- genetics, Blotting, Western, Molecular Sequence Data, Gastric -- immunology, Parietal Cells, Gastric -- immunology, Peptide Fragments -- immunology, Iodide Peroxidase, Epitope Mapping -- methods, Epitopes, 03 medical and health sciences, 0302 clinical medicine, Parietal Cells, Gastric, Thyroid Diseases -- immunology, Reference Values, 80 and over, Humans, Aged, Aged, 80 and over, Base Sequence, Blotting, Parietal Cells, Iodide Peroxidase -- chemistry, Sciences bio-médicales et agricoles, Middle Aged, Thyroid Diseases, Peptide Fragments, Recombinant Proteins, 3. Good health, Molecular Probes, Female, Iodide Peroxidase -- immunology, Western, Epitope Mapping

File Description: No full-text files

Access URL: https://pubmed.ncbi.nlm.nih.gov/7531575
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/56293
https://hdl.handle.net/11568/27923

Sorting and pooling strategy: a novel tool to map a virus proteome for CD8 T-cell epitopes

Authors: Bernhard Ay, Mathias Streitz, Prisca Boisguerin, Andreas Schlosser, Carsten C. Mahrenholz, Sebastian D. Schuck, Florian Kern, Rudolf Volkmer

Subject Terms: Amino Acid Sequence, Biopolymers/chemistry, CD8-Positive T-Lymphocytes/*immunology, Cytomegalovirus/*genetics/*immunology, Epitope Mapping/*methods, Epitopes/*chemistry, Flow Cytometry, Genome, Viral, Humans, Oligopeptides/chemistry, Peptide Mapping, Proteome, Proteomics/*methods

Relation: 10779/uos.23389010.v1

Availability: https://figshare.com/articles/journal_contribution/Sorting_and_pooling_strategy_a_novel_tool_to_map_a_virus_proteome_for_CD8_T-cell_epitopes/23389010

Zebrafish to humans : evolution of the alpha3-chain of type IV collagen and emergence of the autoimmune epitopes associated with Goodpasture syndrome.

Authors: MacDonald, Brian A, Sund, Malin, Grant, Marianne A, Pfaff, Kathleen L, Holthaus, Kathryn, Zon, Leonard I, Kalluri, Raghu

Index Terms: Animals, Anti-Glomerular Basement Membrane Disease/genetics/*immunology, Antibody Specificity/immunology, Autoantibodies/*immunology, Autoantigens/genetics/*immunology, Collagen Type IV/genetics/*immunology, Epitope Mapping/methods, Epitopes/genetics/*immunology, Evolution; Molecular, Humans, Mice, Sequence Homology; Amino Acid, Species Specificity, Structural Homology; Protein, Zebrafish/genetics/immunology, Article in journal, info:eu-repo/semantics/article, text

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-6057
Blood, 0006-4971, 2006, 107:5, s. 1908-1915