A practical consideration for the substrate concentration when determining IC50 values for enzyme inhibition.

Authors: Bearne, Stephen L.^1,2 (AUTHOR) sbearne@dal.ca

Source: Biochemistry & Cell Biology. 12/5/2025, Vol. 103, p1-10. 10p.

Subject Terms: *Inhibitory Concentration 50, *Enzyme kinetics, *Biochemical substrates, *Inhibition (Chemistry), *Enzyme inhibitors

Multimodal CNN-Transformer Fusion With Crossmodal Attention for Automated Insect Identification Using Image and Morphological Features

Authors: Kaur, Simran, Wadhwan, Bhanvi, Rani, Diksha, Taneja, Mohit, Sargam, Ansari, Shaharyar Alam

Source: 2026 International Conference on Computing, Intelligence, and Applications (CIACON) Computing, Intelligence, and Applications (CIACON), 2026 International Conference on. :1-6 Jul, 2026

Relation: 2026 International Conference on Computing, Intelligence, and Applications (CIACON)

Mechanistic Roles of Conserved Active-Site Residues in OXA-48 Carbapenem Hydrolysis

Authors: Taylor, Doris M.^aff1, Ngango, Dignité Fabrice^aff1, Fan, Jiayi^aff1, Park, Suhyeorn^aff1, Sankaran, Banumathi^aff2, Prasad, B. V. Venkataram^aff1, Palzkill, Timothy^aff1

Source: ACS Infectious Diseases. 12(9):3241-3252

Availability: https://pubs.acs.org/doi/10.1021/acsinfecdis.6c00562

Redox regulation in O2 -tolerant [FeFe] hydrogenases: insights from two homologues.

Authors: Khundoker, Rinat¹ (AUTHOR), Majer, Sean¹ (AUTHOR), Silakov, Alexey¹ (AUTHOR) aus40@psu.edu

Source: Journal of Biological Inorganic Chemistry (JBIC). Oct2026, p1-10. 10p.

Subject Terms: *Enzyme kinetics, *Electrochemistry, *Oxidation, *Oxidative stress, *Iron clusters, *Amino acid sequence

Soft Interactions, Rather Than Crowder Size, Determine Deviations From Arrhenius‐Like Kinetics of Phosphofructokinase‐1.

Authors: Rastogi, Harshita^1,2 (AUTHOR), Samanta, Madhav^3,4 (AUTHOR), Nguyen, Truong‐An^3,5 (AUTHOR), Hazrati, Mehrnoosh Khodam³ (AUTHOR), Webb, Bradley A.⁶ (AUTHOR), Pluhařová, Eva³ (AUTHOR) eva.pluharova@jh-inst.cas.cz, Timr, Stepan³ (AUTHOR) stepan.timr@jh-inst.cas.cz, Ebbinghaus, Simon^1,2 (AUTHOR) Simon.Ebbinghaus@rub.de

Source: Angewandte Chemie International Edition. Oct2026, p1. 13p. 6 Illustrations.

Subject Terms: *Phosphofructokinase 1, *Enzyme kinetics, *Chemical kinetics, *Thermal stability, *Cell physiology, *Molecular dynamics

Accessibility and Alignment of Trans‐Cleavage Substrates Modulate the Kinetics of Cas12a Ribonucleoprotein.

Authors: Hu, Jianyu¹ (AUTHOR), Feng, Wei¹ (AUTHOR), Zhang, Hongquan¹ (AUTHOR) hongquan@ualberta.ca, Le, X. Chris¹ (AUTHOR) xc.le@ualberta.ca

Source: Angewandte Chemie International Edition. Oct2026, p1. 11p. 7 Illustrations.

Subject Terms: *Enzyme kinetics, *CRISPRs, *Molecular orientation, *Binding sites, *Nucleoproteins

Multimodal forensic biomarkers of death: gene expression, enzyme kinetics, and histology for PMI estimation.

Authors: Gadepally, Sai Soumya¹ (AUTHOR), Thaker, Khushali¹ (AUTHOR), Patoliya, Jaimini¹ (AUTHOR), Kubhchandani, Harish T.² (AUTHOR), Joshi, Rushikesh¹ (AUTHOR) rushikeshjoshi@gujaratuniversity.ac.in

Source: Australian Journal of Forensic Sciences. Oct2026, Vol. 58 Issue 5, p530-545. 16p.

Subject Terms: *Gene expression, *Enzyme kinetics, *Glutathione peroxidase, *Biomarkers, *Histology, *Oxidative stress, *Glutathione reductase

An enzyme kinetic model for quantitative interpretation of the role of nicotinamide nucleotide transhydrogenase (NNT) in cell physiology.

Authors: Gan, Zhuohui¹ (AUTHOR) ganzoe@protonmail.com, van der Stelt, Inge¹ (AUTHOR) inge.vanderstelt@wur.nl, Grefte, Sander¹ (AUTHOR) sander.grefte@wur.nl, Suarez Diez, Maria² (AUTHOR) maria.suarezdiez@wur.nl, Koopman, Werner J.H.^1,3 (AUTHOR) werner.koopman@radboudumc.nl, Keijer, Jaap¹ (AUTHOR) jaap.keijer@wur.nl

Source: Free Radical Biology & Medicine. Oct2026, Vol. 254, p660-675. 16p.

Subject Terms: *Enzyme kinetics, *Nicotinamide adenine dinucleotide phosphate, *Mitochondrial membranes, *Mathematical models, *Mitochondria, *Homeostasis, *NAD(P)H dehydrogenases

Macaúba (Acrocomia aculeata) pulp oil ethylic biodiesel using lipase fromAspergillus oryzae: kinetic and process parameters.

Authors: Gonçalves, Maria Luiza M. B. B.¹ (AUTHOR), Ferreira, Marcela C.² (AUTHOR), Sampaio, Klicia A.¹ (AUTHOR), Batista, Eduardo A. C.¹ (AUTHOR), Maximo, Guilherme J.¹ (AUTHOR) maximo@unicamp.br

Source: Biofuels, Bioproducts & Biorefining. Sep2026, p1. 13p. 2 Illustrations.

Subject Terms: *Biodiesel fuel manufacturing, *Enzyme kinetics, *Biomass energy, *Lipases, *Vegetable oils, *Ethanol, *Transesterification, *Biodiesel fuels

Insights into the pH-dependent reactivity and substrate induced active-site reorganization of the non- heme iron enzyme CthEgtB.

Authors: Rodriguez, Kassidy W.¹ (AUTHOR) kxr657@miami.edu, Tao, Phuong Hong Ngoc^2,3 (AUTHOR) npt34@miami.edu, Kurmi, Kiran^2,3 (AUTHOR) Kiran.Kurmi@med.miami.edu, Meier, Katlyn K.¹ (AUTHOR) kmeier@miami.edu

Source: Journal of Biological Inorganic Chemistry (JBIC). Sep2026, Vol. 31 Issue 6, p507-519. 13p.

Subject Terms: *Iron proteins, *Enzyme kinetics, *Ligand field theory, *Thermal stability, *Sulfoxides, *Oxygenation (Chemistry)

Structural and functional characterisation of a reconstructed ancestral strigolactone receptor.

Authors: Tuckey, Andrew J.¹ (AUTHOR), Marshall, Andrew C.¹ (AUTHOR), Rothzerg, Emel² (AUTHOR), Monte, Antonella P.¹ (AUTHOR), Bennett, Tom³ (AUTHOR), Bond, Charles S.¹ (AUTHOR), Waters, Mark T.¹ (AUTHOR) mark.waters@uwa.edu.au

Source: Plant Journal. Sep2026, Vol. 127 Issue 5, p1-17. 17p.

Subject Terms: *Enzyme kinetics, *Thermal stability, *Ligands (Chemistry), *Plant hormones, *Drug receptors, *Arabidopsis thaliana, *Molecular evolution, *Protein engineering

Comprehensive metabolic profiling and enzyme kinetics of cis-2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in normal and hyperlipidemic rats.

Authors: Qiu, Jiexin¹ (AUTHOR), Yao, Beibei¹ (AUTHOR), Yu, Qing¹ (AUTHOR), Wang, Jiajie¹ (AUTHOR), Huang, Lingling¹ (AUTHOR), Chen, Jiale¹ (AUTHOR), Lu, Yufeng¹ (AUTHOR), Wang, Chunying^1,2 (AUTHOR) wangchunying@hebmu.edu.cn

Source: Xenobiotica. Sep2026, Vol. 56 Issue 9, p709-721. 13p.

Subject Terms: *Enzyme kinetics, *Cytochrome P-450, *Metabolic profile tests, *Hepatotoxicology, *Hyperlipidemia, *Liver microsomes, *Drug metabolism

Enzyme kinetics shapes the growth response of metabolic networks.

Authors: Seeger, Leon¹ (AUTHOR), Pinheiro, Fernanda² (AUTHOR), Lässig, Michael¹ (AUTHOR) mlaessig@uni-koeln.de

Source: PLoS Computational Biology. 8/19/2026, Vol. 22 Issue 8, p1-32. 32p.

Subject Terms: *Enzyme kinetics, *Microbial metabolism, *Biological networks, *Metabolites, *Protein expression, *Regulation of growth

WILDkCAT: extract, retrieve, and predict enzyme turnover numbers of constraint-based metabolic models.

Authors: Escoffier, Hugues¹ (AUTHOR), Linster, Carole L² (AUTHOR), Sauter, Thomas¹ (AUTHOR)

Source: Bioinformatics. Aug2026, Vol. 42 Issue 8, p1-5. 5p.

Subject Terms: *Enzyme kinetics, *Metabolic models, *Bioinformatics, *Escherichia coli, *Chemical species, *Python programming language

Comparative Quantitative Analysis of Kcat Modulation in Laccases Engineered by Rational, Semi-Rational, and Directed Evolution Approaches.

Authors: Rodríguez-Enríquez, Alan^1,2 (AUTHOR), Rosas-Murrieta, Nora² (AUTHOR) nora.rosas@correo.buap.mx, Torres, Eduardo^1,2 (AUTHOR) eduardo.torres@correo.buap.mx

Source: Catalysts (2073-4344). Aug2026, Vol. 16 Issue 8, p698. 25p.

Subject Terms: *Enzyme kinetics, *Protein engineering, *Laccase, *Mutagenesis, *Biocatalysis

Structure‐Guided Engineering of a Ser192Ala Variant Enhances Bidirectional Catalysis in Amycolatopsis sulphurea L‐Alanine Dehydrogenase.

Authors: Aksu, Tuba Okur¹ (AUTHOR), Aktaş, Fatih² (AUTHOR), Serçinoğlu, Onur¹ (AUTHOR), Binay, Barış^1,3 (AUTHOR) binay@gtu.edu.tr

Source: ChemCatChem. Aug2026, Vol. 18 Issue 15, p1-12. 12p.

Subject Terms: *Catalytic activity, *Enzyme kinetics, *Enzyme biotechnology, *Pyruvates, *Enzymes, *Molecular dynamics

Fluorescent Analysis of K M and V max Values for Methyl-Dependent Restriction Endonucleases.

Authors: Martyshova, Vladislava^1,2 (AUTHOR), Sedykh, Sergey^1,2 (AUTHOR) sirozha@gmail.com

Source: Biomolecules (2218-273X). Aug2026, Vol. 16 Issue 8, p1217. 15p.

Subject Terms: *Enzyme kinetics, *DNA methylation, *Fluorimetry, *DNA restriction enzymes, *Fluorescent probes

Identification and functional characterization of an mRNA Cap3 methyltransferase from Acanthamoeba castellani medusavirusi.

Authors: Takemura, Tokiaki¹ takemura@yamasa.com, Ishige, Kazuya¹

Source: Journal of Biological Chemistry. Aug2026, Vol. 302 Issue 8, p1-12. 12p.

Subject Terms: *Methyltransferases, *Enzyme kinetics, *Enzyme specificity, *RNA modification & restriction, *Chemical kinetics

Kinetic characterization of Streptomyces griseus protease: A comparative study of linear and non-linear regression methods.

Authors: Zarza-Gonzalez, Evelyn A.¹ (AUTHOR), Gonzáles Benítez, Litzy Ayelem¹ (AUTHOR), Cruz Maldonado, Juan Jesús¹ (AUTHOR) juan.jcm@tec.mx

Source: Biocatalysis & Biotransformation. Aug2026, Vol. 44 Issue 4, p299-314. 16p.

Subject Terms: *Enzyme kinetics, *Nonlinear regression, *Enzymology, *Curve fitting, *Monte Carlo method, *Streptomyces griseus

The kinetics of the decomposition of hydrogen peroxide by liver enzyme.

Authors: Oeurn, Sopheak¹ (AUTHOR) oeurn.sopheak@ptec.edu.kh, Khlout, Phakdey¹ (AUTHOR), Reth, Phearun¹ (AUTHOR), Thin, Raksmey¹ (AUTHOR), Konklong, Jittima² (AUTHOR), Maneelam, Pratuengsook² (AUTHOR)

Source: AIP Conference Proceedings. 2026, Vol. 3423 Issue 1, p1-7. 7p.

Subject Terms: *Enzyme kinetics, *Biocatalysis, *Potassium permanganate, *Enzymes, *Heterogeneous catalysis, *Hydrogen peroxide, *Chemical kinetics