Structure of the helicase core of Werner helicase, a key target in microsatellite instability cancers

Authors: Newman, JA, Gavard, AE, Lieb, S, Ravichandran, MC, Hauer, K, Werni, P, Geist, L, Böttcher, J, Engen, JR, Rumpel, K, Samwer, M, Petronczki, M, Gileadi, O

Relation: https://doi.org/10.26508/lsa.202000795

Availability: https://doi.org/10.26508/lsa.202000795
https://ora.ox.ac.uk/objects/uuid:7107d829-8925-4bfd-ace5-90e7cd8c2fa3

Aspartic proteinases in Antarctic fish

Authors: De Luca V, Maria G, De Mauro G, Catara G, Carginale V, Ruggiero G, Capasso A, Parisi E, Brier S, Engen JR, Capasso C

Source: Marine genomics
2 (2009): 1–10. doi:10.1016/j.margen.2009.03.001
info:cnr-pdr/source/autori:De Luca V.; Maria G.; De Mauro G.; Catara G.; Carginale V.; Ruggiero G.; Capasso A.; Parisi E.; Brier S.; Engen J.R.; Capasso C./titolo:Aspartic proteinases in Antarctic fish/doi:10.1016%2Fj.margen.2009.03.001/rivista:Marine genomics (Print)/anno:2009/pagina_da:1/pagina_a:10/intervallo_pagine:1–10/volume:2

Subject Terms: 0301 basic medicine, 0303 health sciences, 03 medical and health sciences, MOLECULAR-CLONING, EVOLUTION, CRYSTAL-STRUCTURES, 14. Life underwater, CATHEPSIN-D, PORCINE PEPSIN

Access URL: https://pubmed.ncbi.nlm.nih.gov/21798166

Purification and characterization of pepsins A1 and A2 from the Antarctic rock cod Trematomus bernacchii

Authors: Brier S, Maria G, Carginale V, Capasso A, Wu Y, Taylor RM, Borotto NB, Capasso C, Engen JR

Source: The FEBS journal
274 (2007): 6152–6166. doi:10.1111/j.1742-4658.2007.06136.x
info:cnr-pdr/source/autori:Brier S.; Maria G.; Carginale V.; Capasso A.; Wu Y.; Taylor R.M.; Borotto N.B.; Capasso C.; Engen J.R./titolo:Purification and characterization of pepsins A1 and A2 from the Antarctic rock cod Trematomus bernacchii/doi:10.1111%2Fj.1742-4658.2007.06136.x/rivista:The FEBS journal (Print)/anno:2007/pagina_da:6152/pagina_a:6166/intervallo_pagine:6152–6166/volume:274

Subject Terms: Models, Molecular, 0301 basic medicine, Molecular Sequence Data, Antarctic Regions, 03 medical and health sciences, cold-adapted protein, Enzyme Stability, Pepstatins, Escherichia coli, Animals, Protease Inhibitors, Amino Acid Sequence, Cloning, Molecular, Inclusion Bodies, Enzyme Precursors, 0303 health sciences, Binding Sites, rock cod, Hydrogen-Ion Concentration, Pepsin A, Perciformes, Isoenzymes, Molecular Weight, Kinetics, Gastric Mucosa, aspartic proteases, specificity

Access URL: https://europepmc.org/articles/pmc2533623?pdf=render
https://pubmed.ncbi.nlm.nih.gov/17976195

Structures of PGAM5 provide insight into active site plasticity and multimeric assembly.

Authors: Chaikuad, A, Filippakopoulos, P, Marcsisin, SR, Picaud, S, Schröder, M, Sekine, S, Ichijo, H, Engen, JR, Takeda, K, Knapp, S

Relation: https://doi.org/10.1016/j.str.2017.05.020

Availability: https://doi.org/10.1016/j.str.2017.05.020
https://ora.ox.ac.uk/objects/uuid:fb44c9b5-211e-4d95-a026-9215d5de7f13

HIV-1 Nef interaction influences the ATP-binding site of the Src-family kinase, Hck

Authors: Pene-Dumitrescu, T, Shu, ST, Wales, TE, Alvarado, JJ, Shi, H, Narute, P, Moroco, JA, Yeh, JI, Engen, JR, Smithgall, TE

Source: BMC Chem Biol

Subject Terms: 0301 basic medicine, 03 medical and health sciences, Drug Discovery, Molecular Medicine, Research Article, 3. Good health

File Description: application/pdf; text/plain

Access URL: https://bmcchembiol.biomedcentral.com/track/pdf/10.1186/1472-6769-12-1
https://pubmed.ncbi.nlm.nih.gov/22420777

Differential Sensitivity of Src-Family Kinases to Activation by SH3 Domain Displacement

Authors: Moroco, JA, Craigo, JK, Iacob, RE, Wales, TE, Engen, JR, Smithgall, TE

Source: PLoS One
PLoS ONE, Vol 9, Iss 8, p e105629 (2014)

Subject Terms: 0301 basic medicine, 0303 health sciences, Science, Enzyme Activation, src Homology Domains, 03 medical and health sciences, src-Family Kinases, Medicine, Humans, Peptides, Research Article, Signal Transduction

File Description: application/pdf; text/plain

Access URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0105629&type=printable
https://pubmed.ncbi.nlm.nih.gov/25144189
https://doaj.org/article/e14dcc7fe6fa4ec0a53c9671ea2bcb36

The Julian-Cuyamaca Bark-Beetle Outbreak - Preliminary Report of Investigative Studies - 1948

Additional Titles: Forest Insect Investigations

Authors: Edward T. Engen, Jr.

Index Terms: California fivespined ips; Ips paraconfusus, Red turpentine beetle; Dendroctonus valens, Western pine beetle; Dendroctonus brevicomis, Bark beetle infestations, Insect outbreaks, Report

URL: http://cdm17053.contentdm.oclc.org/cdm/ref/collection/p17053coll1/id/76281
http://cdm17053.contentdm.oclc.org/utils/getthumbnail/collection/p17053coll1/id/76281
https://worldcat.org/search?q=on:AGM+http://cdm17053.contentdm.oclc.org/oai/oai.php+p17053coll1+CNTCOLL
http://worldcat.org/oclc/1257263839/viewonline

Forest Insect Survey of the Julian-Cuyamaca Insect Zone - Season of 1948

Additional Titles: Forest insect Investigations

Authors: Edward T. Engen, Jr.

Index Terms: Western pine beetle; Dendroctonus brevicomis, Fir engraver; Scolytus ventralis, Coulter pine; Pinus coulteri, Insect infestations, Insect surveys, Memo

URL: http://cdm17053.contentdm.oclc.org/cdm/ref/collection/p17053coll1/id/76323
http://cdm17053.contentdm.oclc.org/utils/getthumbnail/collection/p17053coll1/id/76323
https://worldcat.org/search?q=on:AGM+http://cdm17053.contentdm.oclc.org/oai/oai.php+p17053coll1+CNTCOLL
http://worldcat.org/oclc/1257263837/viewonline

Small Molecule Stabilization of Diverse Amyloidogenic Immunoglobulin Light Chains Revealed by Hydrogen-Deuterium Exchange Mass Spectrometry.

Authors: Peterle D; Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA. Electronic address: daniele@peptone.io., Yan NL; Department of Chemistry, The Scripps Research Institute, La Jolla, CA, USA., Klimtchuk ES; Amyloidosis Center, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA., Srinivasu BY; Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA., Brusic V; Amyloidosis Center, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA., Rhoades D; Department of Chemistry, The Scripps Research Institute, La Jolla, CA, USA., Wales TE; Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA., Gursky O; Department of Pharmacology, Physiology & Biophysics, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA., Kelly JW; Department of Chemistry, The Scripps Research Institute, La Jolla, CA, USA., Engen JR; Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA., Morgan GJ; Amyloidosis Center, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA; Section of Hematology and Medical Oncology, Department of Medicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA. Electronic address: gjmorgan@bu.edu.

Source: Journal of molecular biology [J Mol Biol] 2026 Oct 01; Vol. 438 (19), pp. 169917. Date of Electronic Publication: 2026 Jun 29.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2985088R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1089-8638 (Electronic) Linking ISSN: 00222836 NLM ISO Abbreviation: J Mol Biol Subsets: MEDLINE

MeSH Terms: Immunoglobulin Light Chains*/chemistry , Immunoglobulin Light Chains*/metabolism , Hydrogen Deuterium Exchange-Mass Spectrometry*/methods , Amyloid*/chemistry , Amyloid*/metabolism , Amyloidogenic Proteins*/chemistry , Amyloidogenic Proteins*/metabolism, Immunoglobulin Light-chain Amyloidosis/metabolism ; Humans ; Deuterium Exchange Measurement ; Protein Stability ; Protein Binding ; Protein Conformation ; Mass Spectrometry ; Proteolysis

Magnesium as a conformational gatekeeper of KRAS: Structural dynamics and therapeutic implications.

Authors: Srinivasu BY; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA., Damerla TS; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA., Stec A; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA., Zhou Z; Department of Radiation Oncology, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA.; Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA., Engen JR; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA., Westover KD; Department of Radiation Oncology, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA.; Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA., Wales TE; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA.

Source: Protein science : a publication of the Protein Society [Protein Sci] 2026 Jul; Vol. 35 (7), pp. e70681.

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.

Journal Info: Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9211750 Publication Model: Print Cited Medium: Internet ISSN: 1469-896X (Electronic) Linking ISSN: 09618368 NLM ISO Abbreviation: Protein Sci Subsets: MEDLINE

MeSH Terms: Proto-Oncogene Proteins p21(ras)*/chemistry , Proto-Oncogene Proteins p21(ras)*/metabolism , Proto-Oncogene Proteins p21(ras)*/genetics , Magnesium*/chemistry , Magnesium*/metabolism , SOS1 Protein*/chemistry , SOS1 Protein*/metabolism , SOS1 Protein*/genetics, Guanosine Triphosphate/metabolism ; Guanosine Triphosphate/chemistry ; Guanosine Diphosphate/metabolism ; Guanosine Diphosphate/chemistry ; Humans ; Protein Conformation ; Hydrogen Deuterium Exchange-Mass Spectrometry

A druggable redox switch on SHP1 controls macrophage inflammation.

Authors: Ng MY; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Department of Cell Biology, Harvard Medical School, Boston, MA, USA., Nix MN; Department of Chemical and Systems Biology, CHEM-H and SCI, Stanford Medical School, Stanford University, Stanford, CA, USA.; Department of Chemistry, Stanford University, Stanford, CA, USA., Du G; Department of Chemical and Systems Biology, CHEM-H and SCI, Stanford Medical School, Stanford University, Stanford, CA, USA., Davidek I; Department of Chemical and Systems Biology, CHEM-H and SCI, Stanford Medical School, Stanford University, Stanford, CA, USA., Burger N; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Department of Cell Biology, Harvard Medical School, Boston, MA, USA., Shin S; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Department of Cell Biology, Harvard Medical School, Boston, MA, USA., Toenjes S; Department of Chemical and Systems Biology, CHEM-H and SCI, Stanford Medical School, Stanford University, Stanford, CA, USA., Takeda H; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Department of Cell Biology, Harvard Medical School, Boston, MA, USA., Cheah Xin Yan M; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Department of Cell Biology, Harvard Medical School, Boston, MA, USA., Zhang B; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Department of Cell Biology, Harvard Medical School, Boston, MA, USA., Xiao H; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Department of Cell Biology, Harvard Medical School, Boston, MA, USA., Wei SM; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Department of Cell Biology, Harvard Medical School, Boston, MA, USA., Seo HS; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA., Dhe-Paganon S; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA., Wales TE; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA., Engen JR; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA., Mills EL; Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA, USA.; Department of Immunology, Harvard Medical School, Boston, MA, USA., Che J; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA. jianwei_che@dfci.harvard.edu., Zhang T; Department of Chemical and Systems Biology, CHEM-H and SCI, Stanford Medical School, Stanford University, Stanford, CA, USA. ztinghu8@stanford.edu., Gray NS; Department of Chemical and Systems Biology, CHEM-H and SCI, Stanford Medical School, Stanford University, Stanford, CA, USA. nsgray01@stanford.edu., Chouchani ET; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA. edwardt_chouchani@dfci.harvard.edu.; Department of Cell Biology, Harvard Medical School, Boston, MA, USA. edwardt_chouchani@dfci.harvard.edu.; Howard Hughes Medical Institute, Chevy Chase, MD, USA. edwardt_chouchani@dfci.harvard.edu.

Source: Nature chemical biology [Nat Chem Biol] 2026 Jul; Vol. 22 (7), pp. 1187-1200. Date of Electronic Publication: 2026 Mar 12.

Publication Type: Journal Article

Journal Info: Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101231976 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-4469 (Electronic) Linking ISSN: 15524450 NLM ISO Abbreviation: Nat Chem Biol Subsets: MEDLINE

MeSH Terms: Protein Tyrosine Phosphatase, Non-Receptor Type 6*/metabolism , Protein Tyrosine Phosphatase, Non-Receptor Type 6*/chemistry , Macrophages*/drug effects , Macrophages*/metabolism , Macrophages*/immunology , Inflammation*/metabolism , Inflammation*/drug therapy, Cysteine/metabolism ; Cysteine/chemistry ; Cytokines/metabolism ; Signal Transduction/drug effects ; Oxidation-Reduction ; Animals ; Humans ; Mice

Correction: Dynamic conformational equilibria in the active states of KRAS and NRAS.

Authors: Rennella E; Department of Biochemistry, University of Toronto Toronto Canada., Henry C; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Dickson CJ; Novartis Biomedical Research Cambridge MA USA., Georgescauld F; Department of Chemistry and Chemical Biology, Northeastern University Boston MA USA., Wales TE; Department of Chemistry and Chemical Biology, Northeastern University Boston MA USA., Erdmann D; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Cotesta S; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Maira M; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Sedrani R; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Brachmann SM; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Ostermann N; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Engen JR; Department of Chemistry and Chemical Biology, Northeastern University Boston MA USA., Kay LE; Department of Biochemistry, University of Toronto Toronto Canada., Beyer KS; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Wilcken R; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Jahnke W; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com.

Source: RSC chemical biology [RSC Chem Biol] 2026 May 05; Vol. 7 (6), pp. 1120-1121. Date of Electronic Publication: 2026 May 05 (Print Publication: 2026).

Publication Type: Published Erratum

Journal Info: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101768727 Publication Model: eCollection Cited Medium: Internet ISSN: 2633-0679 (Electronic) Linking ISSN: 26330679 NLM ISO Abbreviation: RSC Chem Biol Subsets: PubMed not MEDLINE

Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level.

Authors: Roeselová A; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK., Pajak A; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK., Wales TE; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA., Pellowe GA; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK.; Aston Institute for Membrane Excellence, Aston University, Birmingham, UK., Kjær S; Structural Biology Science Technology Platform, The Francis Crick Institute, London, UK., Engen JR; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA. j.engen@northeastern.edu., Balchin D; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK. david.balchin@crick.ac.uk.

Source: Nature protocols [Nat Protoc] 2026 May; Vol. 21 (5), pp. 2271-2300. Date of Electronic Publication: 2026 Jan 12.

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural

Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE

MeSH Terms: Peptides*/chemistry , Peptides*/isolation & purification , Peptides*/metabolism , Ribosomes*/chemistry , Ribosomes*/metabolism , Hydrogen Deuterium Exchange-Mass Spectrometry* , Protein Biosynthesis*, Escherichia coli Proteins/biosynthesis ; Escherichia coli Proteins/chemistry ; Escherichia coli Proteins/isolation & purification ; Protein Conformation ; Protein Folding ; Ultracentrifugation

How the non-motile kinesin KIF7 adapts conserved kinesin principles for its function in Hedgehog signaling.

Authors: Haque F; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA., Srinivasu BY; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA., Engen JR; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA., Wales TE; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA., Subramanian R; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

Source: BioRxiv : the preprint server for biology [bioRxiv] 2026 Feb 10. Date of Electronic Publication: 2026 Feb 10.

Publication Type: Journal Article; Preprint

Journal Info: Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet ISSN: 2692-8205 (Electronic) Linking ISSN: 26928205 NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE

Inhibition of oligomeric BAX by an anti-apoptotic dimer.

Authors: Newman CE; Department of Pediatric Oncology and Chemical Biology Program, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Gygi MA; Department of Pediatric Oncology and Chemical Biology Program, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Alimohamadi H; Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, USA., DeAngelo TM; Department of Pediatric Oncology and Chemical Biology Program, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Camara CM; Department of Pediatric Oncology and Chemical Biology Program, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Mintseris J; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA., Yu E; Department of Pediatric Oncology and Chemical Biology Program, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Harvey EP; Department of Pediatric Oncology and Chemical Biology Program, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Hauseman ZJ; Department of Pediatric Oncology and Chemical Biology Program, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Fan L; SAXS Facility, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA., Wang YX; Center for Structural Biology, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA., Luo EW; Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, USA., Godes M; Department of Pediatric Oncology and Chemical Biology Program, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Gehtman J; Department of Pediatric Oncology and Chemical Biology Program, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Cathcart AM; Department of Pediatric Oncology and Chemical Biology Program, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Gygi SP; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA., Engen JR; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA., Bird GH; Department of Pediatric Oncology and Chemical Biology Program, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Wong GCL; Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, USA., Wales TE; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA., Walensky LD; Department of Pediatric Oncology and Chemical Biology Program, Dana-Farber Cancer Institute, Boston, MA 02215, USA. Electronic address: loren_walensky@dfci.harvard.edu.

Source: Cell [Cell] 2025 Dec 24; Vol. 188 (26), pp. 7397-7412.e21. Date of Electronic Publication: 2025 Nov 21.

Publication Type: Journal Article

Journal Info: Publisher: Cell Press Country of Publication: United States NLM ID: 0413066 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4172 (Electronic) Linking ISSN: 00928674 NLM ISO Abbreviation: Cell Subsets: MEDLINE

MeSH Terms: bcl-2-Associated X Protein*/metabolism , bcl-2-Associated X Protein*/chemistry , bcl-2-Associated X Protein*/antagonists & inhibitors , Apoptosis*, Mitochondria/metabolism ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Proto-Oncogene Proteins c-bcl-2/chemistry ; Humans ; Protein Multimerization ; Scattering, Small Angle ; Animals

Rigorous Analysis of Multimodal HDX-MS Spectra.

Authors: Tuttle LM; Department of Biochemistry, University of Washington, Seattle, Washington 98195, United States., James EI; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, United States., Georgescauld F; Cell Signaling Technology, Danvers, Massachusetts 01923, United States., Wales TE; Department of Chemistry & Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States., Weis DD; Department of Chemistry, The University of Kansas, Lawrence, Kansas 66045, United States., Engen JR; Department of Chemistry & Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States., Nath A; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, United States., Klevit RE; Department of Biochemistry, University of Washington, Seattle, Washington 98195, United States., Guttman M; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, United States.

Source: Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2025 Feb 05; Vol. 36 (2), pp. 416-423. Date of Electronic Publication: 2025 Jan 21.

Publication Type: Journal Article

Journal Info: Publisher: ACS Publications Country of Publication: United States NLM ID: 9010412 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1123 (Electronic) Linking ISSN: 10440305 NLM ISO Abbreviation: J Am Soc Mass Spectrom Subsets: MEDLINE

MeSH Terms: Proteins*/chemistry , Hydrogen Deuterium Exchange-Mass Spectrometry*/methods, Peptides/chemistry ; Algorithms ; Software

An SH3-binding allosteric modulator stabilizes the global conformation of the AML-associated Src-family kinase, Hck.

Authors: Selzer AM; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA., Gerlach G; Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA., Gonzalez-Areizaga G; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA., Wales TE; Department of Chemistry and Chemical Biology, College of Science, Northeastern University, Boston, Massachusetts, USA., Cui SY; Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, Pennsylvania, USA., Iyer P; Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, Pennsylvania, USA., Engen JR; Department of Chemistry and Chemical Biology, College of Science, Northeastern University, Boston, Massachusetts, USA., Camacho C; Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA., Ishima R; Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania., Smithgall TE; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA. Electronic address: tsmithga@pitt.edu.

Source: The Journal of biological chemistry [J Biol Chem] 2025 Jan; Vol. 301 (1), pp. 108088. Date of Electronic Publication: 2024 Dec 13.

Publication Type: Journal Article; Research Support, N.I.H., Extramural

Journal Info: Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE

MeSH Terms: Proto-Oncogene Proteins c-hck*/chemistry , Proto-Oncogene Proteins c-hck*/metabolism , Proto-Oncogene Proteins c-hck*/genetics , Proto-Oncogene Proteins c-hck*/antagonists & inhibitors , Leukemia, Myeloid, Acute*/enzymology , Leukemia, Myeloid, Acute*/drug therapy , Leukemia, Myeloid, Acute*/genetics , Protein Kinase Inhibitors*/pharmacology , Protein Kinase Inhibitors*/chemistry , Pyrimidines*/pharmacology , Pyrimidines*/chemistry , src Homology Domains*, Allosteric Regulation/drug effects ; Humans ; Protein Conformation ; Molecular Dynamics Simulation ; Binding Sites

Impact of the clinically approved BTK inhibitors on the conformation of full-length BTK and analysis of the development of BTK resistance mutations in chronic lymphocytic leukemia.

Authors: Joseph RE; Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, United States., Wales TE; Department of Chemistry and Chemical Biology, Northeastern University, Boston, United States., Jayne S; The Ernest and Helen Scott Haematological Research Institute, Leicester Cancer Research Centre, College of Life Sciences, University of Leicester, Leicester, United Kingdom., Britton RG; The Ernest and Helen Scott Haematological Research Institute, Leicester Cancer Research Centre, College of Life Sciences, University of Leicester, Leicester, United Kingdom., Fulton DB; Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, United States., Engen JR; Department of Chemistry and Chemical Biology, Northeastern University, Boston, United States., Dyer MJS; The Ernest and Helen Scott Haematological Research Institute, Leicester Cancer Research Centre, College of Life Sciences, University of Leicester, Leicester, United Kingdom., Andreotti AH; Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, United States.

Source: ELife [Elife] 2024 Dec 27; Vol. 13. Date of Electronic Publication: 2024 Dec 27.

Publication Type: Journal Article

Journal Info: Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE

MeSH Terms: Agammaglobulinaemia Tyrosine Kinase*/antagonists & inhibitors , Agammaglobulinaemia Tyrosine Kinase*/genetics , Agammaglobulinaemia Tyrosine Kinase*/chemistry , Agammaglobulinaemia Tyrosine Kinase*/metabolism , Leukemia, Lymphocytic, Chronic, B-Cell*/drug therapy , Leukemia, Lymphocytic, Chronic, B-Cell*/genetics , Protein Kinase Inhibitors*/pharmacology , Protein Kinase Inhibitors*/chemistry , Piperidines*/pharmacology , Piperidines*/chemistry , Adenine*/analogs & derivatives , Adenine*/pharmacology , Adenine*/chemistry , Adenine*/metabolism , Pyrimidines*/pharmacology , Pyrimidines*/chemistry , Pyrazoles*/pharmacology , Pyrazoles*/chemistry , Drug Resistance, Neoplasm*/genetics , Mutation*, Pyrazines/pharmacology ; Pyrazines/chemistry ; Benzamides/pharmacology ; Humans ; Protein Conformation ; Imidazoles

Resolving chaperone-assisted protein folding on the ribosome at the peptide level.

Authors: Wales TE; Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA., Pajak A; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK., Roeselová A; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK., Shivakumaraswamy S; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK., Howell S; Proteomics Science Technology Platform, The Francis Crick Institute, London, UK., Kjær S; Structural Biology Science Technology Platform, The Francis Crick Institute, London, UK., Hartl FU; Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Martinsried, Germany., Engen JR; Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA. j.engen@northeastern.edu., Balchin D; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK. david.balchin@crick.ac.uk.

Source: Nature structural & molecular biology [Nat Struct Mol Biol] 2024 Dec; Vol. 31 (12), pp. 1888-1897. Date of Electronic Publication: 2024 Jul 10.

Publication Type: Journal Article

Journal Info: Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101186374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1545-9985 (Electronic) Linking ISSN: 15459985 NLM ISO Abbreviation: Nat Struct Mol Biol Subsets: MEDLINE

MeSH Terms: Ribosomes*/metabolism , Escherichia coli*/metabolism , Escherichia coli Proteins*/metabolism , Escherichia coli Proteins*/chemistry , Molecular Chaperones*/metabolism , Molecular Chaperones*/chemistry , Tetrahydrofolate Dehydrogenase*/metabolism , Tetrahydrofolate Dehydrogenase*/chemistry , Protein Folding*, Ribosomal Proteins/metabolism ; Ribosomal Proteins/chemistry ; Peptides/metabolism ; Peptides/chemistry ; Models, Molecular ; Protein Conformation ; Protein Biosynthesis ; Peptidylprolyl Isomerase

Dynamic conformational equilibria in the active states of KRAS and NRAS.

Authors: Rennella E; University of Toronto, Department of Biochemistry Toronto Canada., Henry C; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Dickson CJ; Novartis Biomedical Research Cambridge MA USA., Georgescauld F; Northeastern University, Department of Chemistry and Chemical Biology Boston MA USA., Wales TE; Northeastern University, Department of Chemistry and Chemical Biology Boston MA USA., Erdmann D; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Cotesta S; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Maira M; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Sedrani R; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Brachmann SM; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Ostermann N; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Engen JR; Northeastern University, Department of Chemistry and Chemical Biology Boston MA USA., Kay LE; University of Toronto, Department of Biochemistry Toronto Canada., Beyer KS; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Wilcken R; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com., Jahnke W; Novartis Biomedical Research Basel Switzerland wolfgang.jahnke@novartis.com rwilcken@flaretx.com kim.beyer@novartis.com.

Source: RSC chemical biology [RSC Chem Biol] 2024 Nov 25; Vol. 6 (1), pp. 106-118. Date of Electronic Publication: 2024 Nov 25 (Print Publication: 2025).

Publication Type: Journal Article

Journal Info: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101768727 Publication Model: eCollection Cited Medium: Internet ISSN: 2633-0679 (Electronic) Linking ISSN: 26330679 NLM ISO Abbreviation: RSC Chem Biol Subsets: PubMed not MEDLINE