A clinically compatible in vitro drug-screening platform identifies therapeutic vulnerabilities in primary cultures of brain metastases

Authors: Jeising, Sebastian, Nickel, Ann-Christin, Trübel, Johanna, Felsberg, Jörg, Picard, Daniel, Leprivier, Gabriel, Wolter, Marietta, Huynh, My Ky, Olivera, Marlene B., Kaulich, Kerstin, Häberle, Lena, Esposito, Irene, Klau, Gunnar W., Steinmann, Julia, Beez, Thomas, Rapp, Marion, Sabel, Michael, Dietrich, Sascha, Remke, Marc, Cornelius, Jan F., Reifenberger, Guido, Qin, Nan

Source: J Neurooncol

Subject Terms: Female [MeSH], Brain Neoplasms/secondary [MeSH], Spheroid cultures, Brain Neoplasms/genetics [MeSH], Humans [MeSH], Antineoplastic Agents/pharmacology [MeSH], Middle Aged [MeSH], Tumor Cells, Cultured [MeSH], Personalized medicine, Drug Screening Assays, Antitumor/methods [MeSH], High-Throughput Screening Assays/methods [MeSH], Male [MeSH], High-throughput drug screening, Research, Brain metastasis, Antineoplastic Agents/therapeutic use [MeSH], Brain Neoplasms/drug therapy [MeSH], Brain Neoplasms/pathology [MeSH], High-Throughput Nucleotide Sequencing [MeSH], Male, 0301 basic medicine, Brain Neoplasms, High-Throughput Nucleotide Sequencing, Antineoplastic Agents, Middle Aged, High-Throughput Screening Assays, 03 medical and health sciences, 0302 clinical medicine, Tumor Cells, Cultured, Humans, Female, Drug Screening Assays, Antitumor

Access URL: https://pubmed.ncbi.nlm.nih.gov/38985431
https://repository.publisso.de/resource/frl:6498549

A spheroid whole mount drug testing pipeline with machine-learning based image analysis identifies cell-type specific differences in drug efficacy on a single-cell level

Authors: Vitacolonna, Mario, Bruch, Roman, Schneider, Richard, Jabs, Julia, Hafner, Mathias, Reischl, Markus, Rudolf, Rüdiger

Source: BMC Cancer
BMC Cancer, Vol 24, Iss 1, Pp 1-23 (2024)
BMC Cancer, 24 (1), Art.-Nr.: 1542

Subject Terms: ddc:004, 0301 basic medicine, Antineoplastic Agents, Apoptosis, Machine Learning, 03 medical and health sciences, Imaging, Three-Dimensional, 3D drug testing, Spheroids, Cellular, Cell Line, Tumor, Image Processing, Computer-Assisted, Humans, Cell Line, Tumor [MeSH], Microscopy, Confocal/methods [MeSH], Fibroblasts/drug effects [MeSH], Humans [MeSH], Apoptosis/drug effects [MeSH], Antineoplastic Agents/pharmacology [MeSH], Tumor microenvironment, Drug resistance, Spheroids, Cellular/drug effects [MeSH], 3D co-culture, Image Processing, Computer-Assisted/methods [MeSH], Drug Screening Assays, Antitumor/methods [MeSH], Single-cell analysis, Doxorubicin/pharmacology [MeSH], Single-Cell Analysis/methods [MeSH], Research, Machine Learning [MeSH], Coculture Techniques [MeSH], Imaging, Three-Dimensional/methods [MeSH], Deep-learning image analysis, Cell Proliferation/drug effects [MeSH], Fibroblasts/metabolism [MeSH], RC254-282, Cell Proliferation, 0303 health sciences, Microscopy, Confocal, DATA processing & computer science, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Fibroblasts, Coculture Techniques, Doxorubicin, Single-Cell Analysis, Drug Screening Assays, Antitumor

File Description: application/pdf

Access URL: https://pubmed.ncbi.nlm.nih.gov/39696122
https://doaj.org/article/94688b951250495fb6faafe8830e39c4
https://repository.publisso.de/resource/frl:6497322

Cancer research using organoid technology

Authors: Kai Kretzschmar

Source: J Mol Med (Berl)

Subject Terms: 0301 basic medicine, ddc:610, 0303 health sciences, Carcinogenesis, DNA Mutational Analysis, Epithelial Cells, Review, Medical Oncology, Stem Cell Research, 3. Good health, Organoids, Translational Research, Biomedical, 03 medical and health sciences, Cell Transformation, Neoplastic, Neoplasms, Stem Cell Research [MeSH], Cancer, Organoids/drug effects [MeSH], Medical Oncology/trends [MeSH], Biological Specimen Banks [MeSH], Neoplastic Stem Cells/cytology [MeSH], Translational Research, Biomedical/methods [MeSH], DNA Mutational Analysis [MeSH], Drug Screening Assays, Antitumor/methods [MeSH], Neoplasms/pathology [MeSH], Organoids/cytology [MeSH], Immuno-oncology, Pre-clinical models, Drug Screening Assays, Antitumor/trends [MeSH], Personalised medicine, 3D culture, Translational Research, Biomedical/trends [MeSH], Humans [MeSH], Medical Oncology/methods [MeSH], Neoplasm Metastasis [MeSH], Neoplasms/immunology [MeSH], Cell Transformation, Neoplastic/genetics [MeSH], Neoplasms/genetics [MeSH], Genes, Neoplasm [MeSH], Epithelial Cells/cytology [MeSH], Forecasting [MeSH], Neoplasms/drug therapy [MeSH], Drug screening, Carcinogenesis [MeSH], Tumor Microenvironment [MeSH], Neoplastic Stem Cells, Tumor Microenvironment, Humans, Drug Screening Assays, Antitumor, Neoplasm Metastasis, Biological Specimen Banks, Forecasting, Genes, Neoplasm

File Description: application/pdf

Access URL: https://link.springer.com/content/pdf/10.1007/s00109-020-01990-z.pdf
https://pubmed.ncbi.nlm.nih.gov/33057820
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026469
https://link.springer.com/article/10.1007/s00109-020-01990-z
https://europepmc.org/article/PMC/PMC8026469
https://pubmed.ncbi.nlm.nih.gov/33057820/
https://link.springer.com/content/pdf/10.1007/s00109-020-01990-z.pdf
https://repository.publisso.de/resource/frl:6443070
https://opus.bibliothek.uni-wuerzburg.de/files/23537/Kretzschmar2021_Article_CancerResearchUsingOrganoidTec.pdf
https://doi.org/10.1007/s00109-020-01990-z
https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-235377
https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/23537

Dichloroacetate and PX-478 Exhibit Strong Synergistic Effects in a Various Number of Cancer Cell Lines

Authors: Jonas Parczyk, Jérôme Ruhnau, Carsten Pelz, Max Schilling, Hao Wu, Nicole Nadine Piaskowski, Britta Eickholt, Hartmut Kühn, Kerstin Danker, Andreas Klein

Source: BMC Cancer
BMC Cancer, Vol 21, Iss 1, Pp 1-14 (2021)

Subject Terms: 0301 basic medicine, Mustard Compounds, Cancer therapy, Cell Survival, Cancer cell lines, Apoptosis, Dichloroacetate, HIF-1α inhibition, PX-478, 03 medical and health sciences, 0302 clinical medicine, Cell Line, Tumor, Antineoplastic Combined Chemotherapy Protocols, Humans, Drug combination, RC254-282, Cell Proliferation, 2. Zero hunger, Dichloroacetic Acid, Dose-Response Relationship, Drug, Phenylpropionates, HIF-1 alpha, Synergism, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Drug Synergism, Cell Cycle Checkpoints, inhibition, 3. Good health, Cell Line, Tumor/drug effects [MeSH], Drug Synergism [MeSH], Phenylpropionates/pharmacology [MeSH], Experimental therapeutics and drug development, Drug Screening Assays, Antitumor/methods [MeSH], Dichloroacetic Acid/pharmacology [MeSH], MCF-7 Cells [MeSH], Metabolism, Research Article, A549 Cells [MeSH], Dose-Response Relationship, Drug [MeSH], Humans [MeSH], Reactive Oxygen Species/metabolism [MeSH], Cell Cycle Checkpoints/drug effects [MeSH], Apoptosis/drug effects [MeSH], Cell Survival/drug effects [MeSH], HT29 Cells [MeSH], Antineoplastic Combined Chemotherapy Protocols/pharmacology [MeSH], Cell Proliferation/drug effects [MeSH], HeLa Cells [MeSH], Mustard Compounds/pharmacology [MeSH], A549 Cells, MCF-7 Cells, Drug Screening Assays, Antitumor, Reactive Oxygen Species, HT29 Cells, 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit, HeLa Cells

Access URL: https://www.researchsquare.com/article/rs-88933/v1.pdf
https://bmccancer.biomedcentral.com/track/pdf/10.1186/s12885-021-08186-9
https://pubmed.ncbi.nlm.nih.gov/33931028
https://doaj.org/article/d088ebf1f5634ab2825caf74af90cb4a
https://bmccancer.biomedcentral.com/articles/10.1186/s12885-021-08186-9
https://link.springer.com/content/pdf/10.1186/s12885-021-08186-9.pdf
https://link.springer.com/article/10.1186/s12885-021-08186-9
https://pubmed.ncbi.nlm.nih.gov/33931028/
https://europepmc.org/article/MED/33931028
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086110
https://repository.publisso.de/resource/frl:6462811
https://refubium.fu-berlin.de/handle/fub188/38446
https://doi.org/10.17169/refubium-38164
https://doi.org/10.1186/s12885-021-08186-9