Lipid Modification and Membrane Localization of Proteins in Cell-Free System.

Συγγραφείς: Matsumoto R; GeneFrontier Corporation, 273-1 Kashiwa, Kashiwa, Chiba 277-0005, Japan., Niwa T; Cell Biology Center, Institute of Integrated Research, Institute of Science Tokyo, Yokohama, Kanagawa 226-8501, Japan., Kuno K; Institute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan., Shimane Y; Institute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan., Kuruma Y; Institute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan., Kanamori T; GeneFrontier Corporation, 273-1 Kashiwa, Kashiwa, Chiba 277-0005, Japan.

Πηγή: ACS synthetic biology [ACS Synth Biol] 2025 Jul 18; Vol. 14 (7), pp. 2729-2738. Date of Electronic Publication: 2025 Jun 19.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Cell-Free System*/metabolism , Lipoylation* , Protein Processing, Post-Translational*, Humans ; Acyltransferases/metabolism ; Cell Membrane/metabolism ; Liposomes/metabolism ; Liposomes/chemistry ; Myristic Acid/metabolism ; Single-Domain Antibodies/metabolism

A Critical View on the Use of DNA Hydrogels in Cell-Free Protein Synthesis.

Συγγραφείς: Moench S; Institute for Biological Interfaces (IBG-1), Karlsruhe Institute of Technology (KIT), 76344, Eggenstein-Leopoldshafen, Germany., Lemke P; Institute for Biological Interfaces (IBG-1), Karlsruhe Institute of Technology (KIT), 76344, Eggenstein-Leopoldshafen, Germany., Hansen A; Institute for Biological Interfaces (IBG-1), Karlsruhe Institute of Technology (KIT), 76344, Eggenstein-Leopoldshafen, Germany., Bickmann C; Institute for Biological Interfaces (IBG-1), Karlsruhe Institute of Technology (KIT), 76344, Eggenstein-Leopoldshafen, Germany., Peng M; Institute for Biological Interfaces (IBG-1), Karlsruhe Institute of Technology (KIT), 76344, Eggenstein-Leopoldshafen, Germany., Rabe KS; Institute for Biological Interfaces (IBG-1), Karlsruhe Institute of Technology (KIT), 76344, Eggenstein-Leopoldshafen, Germany., Domínguez CM; Institute for Biological Interfaces (IBG-1), Karlsruhe Institute of Technology (KIT), 76344, Eggenstein-Leopoldshafen, Germany., Niemeyer CM; Institute for Biological Interfaces (IBG-1), Karlsruhe Institute of Technology (KIT), 76344, Eggenstein-Leopoldshafen, Germany.

Πηγή: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2025 Jan 10; Vol. 64 (2), pp. e202414480. Date of Electronic Publication: 2024 Nov 12.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 0370543 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-3773 (Electronic) Linking ISSN: 14337851 NLM ISO Abbreviation: Angew Chem Int Ed Engl Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Cell-Free System* , DNA*/chemistry , Hydrogels*/chemistry , Hydrogels*/chemical synthesis, Protein Biosynthesis ; Proteins/chemistry

Cell-Free Gene Expression: Methods and Applications.

Συγγραφείς: Hunt AC; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States.; Center for Synthetic Biology, Northwestern University, Evanston, Illinois 60208, United States., Rasor BJ; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States.; Center for Synthetic Biology, Northwestern University, Evanston, Illinois 60208, United States., Seki K; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States.; Center for Synthetic Biology, Northwestern University, Evanston, Illinois 60208, United States., Ekas HM; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States.; Center for Synthetic Biology, Northwestern University, Evanston, Illinois 60208, United States., Warfel KF; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States.; Center for Synthetic Biology, Northwestern University, Evanston, Illinois 60208, United States., Karim AS; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States.; Center for Synthetic Biology, Northwestern University, Evanston, Illinois 60208, United States., Jewett MC; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States.; Center for Synthetic Biology, Northwestern University, Evanston, Illinois 60208, United States.; Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois 60208, United States.; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois 60611, United States.; Department of Bioengineering, Stanford University, Stanford, California 94305, United States.

Πηγή: Chemical reviews [Chem Rev] 2025 Jan 08; Vol. 125 (1), pp. 91-149. Date of Electronic Publication: 2024 Dec 19.

Τύπος έκδοσης: Journal Article; Review

Στοιχεία περιοδικού: Publisher: American Chemical Society Country of Publication: United States NLM ID: 2985134R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6890 (Electronic) Linking ISSN: 00092665 NLM ISO Abbreviation: Chem Rev Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Cell-Free System*/metabolism, Gene Expression ; Synthetic Biology/methods ; Humans

A cell-free bacteriophage synthesis system for directed evolution.

Συγγραφείς: Xu B; School of Basic Medical Sciences, Hubei University of Science and Technology, Xianning 437100, PR China. Electronic address: xub@whu.edu.cn., Liu LH; Tidetron Bioworks Technology (Guangzhou) Co., Ltd, Guangzhou Qianxiang Bioworks Co., Ltd., Guangzhou, Guangdong 510000, PR China., Lin H; Tidetron Bioworks Technology (Guangzhou) Co., Ltd, Guangzhou Qianxiang Bioworks Co., Ltd., Guangzhou, Guangdong 510000, PR China., Zhang Y; Tidetron Bioworks Technology (Guangzhou) Co., Ltd, Guangzhou Qianxiang Bioworks Co., Ltd., Guangzhou, Guangdong 510000, PR China., Huang Y; Tidetron Bioworks Technology (Guangzhou) Co., Ltd, Guangzhou Qianxiang Bioworks Co., Ltd., Guangzhou, Guangdong 510000, PR China., He Q; Tidetron Bioworks Technology (Guangzhou) Co., Ltd, Guangzhou Qianxiang Bioworks Co., Ltd., Guangzhou, Guangdong 510000, PR China., Wang F; Tidetron Bioworks Technology (Guangzhou) Co., Ltd, Guangzhou Qianxiang Bioworks Co., Ltd., Guangzhou, Guangdong 510000, PR China., Wu YR; Tidetron Bioworks Technology (Guangzhou) Co., Ltd, Guangzhou Qianxiang Bioworks Co., Ltd., Guangzhou, Guangdong 510000, PR China., Zhang Z; Tidetron Bioworks Technology (Guangzhou) Co., Ltd, Guangzhou Qianxiang Bioworks Co., Ltd., Guangzhou, Guangdong 510000, PR China. Electronic address: worthy@tidetronbio.com., Jiang A; Tidetron Bioworks Technology (Guangzhou) Co., Ltd, Guangzhou Qianxiang Bioworks Co., Ltd., Guangzhou, Guangdong 510000, PR China. Electronic address: jiangao@tidetronbio.com.

Πηγή: Trends in biotechnology [Trends Biotechnol] 2025 Jan; Vol. 43 (1), pp. 248-261. Date of Electronic Publication: 2024 Oct 26.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Elsevier Science Publishers Country of Publication: England NLM ID: 8310903 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3096 (Electronic) Linking ISSN: 01677799 NLM ISO Abbreviation: Trends Biotechnol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Directed Molecular Evolution*/methods , Cell-Free System*, DNA-Directed RNA Polymerases/genetics ; DNA-Directed RNA Polymerases/metabolism ; Bacteriophage M13/genetics ; Bacteriophage M13/metabolism ; RNA, Transfer/genetics ; RNA, Transfer/metabolism ; Viral Proteins/genetics ; Viral Proteins/metabolism ; Plasmids/genetics ; Genome, Viral/genetics ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Gene Library

Modular Golden Gate Assembly of Linear DNA Templates for Cell-Free Prototyping.

Συγγραφείς: Lehr FX; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany., Gaizauskaite A; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany.; Synthetic Biology, TUM Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Straubing, Germany., Lipińska KE; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany., Gilles S; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany., Sahoo A; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany., Inckemann R; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany., Niederholtmeyer H; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany. henrike.niederholtmeyer@tum.de.; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany. henrike.niederholtmeyer@tum.de.; Synthetic Biology, TUM Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Straubing, Germany. henrike.niederholtmeyer@tum.de.

Πηγή: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2850, pp. 197-217.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Cell-Free System* , Synthetic Biology*/methods , Promoter Regions, Genetic*, DNA/genetics ; DNA/chemistry ; Transcription, Genetic ; Cloning, Molecular/methods ; Protein Biosynthesis ; Polymerase Chain Reaction/methods ; Templates, Genetic ; Binding Sites

Heterologous expression of an aminopeptidase in Escherichia coli for cell-free biosynthesis of l-carnosine.

Συγγραφείς: Zhu Z; School of Biological and Food Engineering, SuZhou University of Technology, 99 South Third Ring Road, Suzhou, Jiangsu Province, 215500, China; College of Light Industry and Food Engineering, Guangxi University, 100 Daxue Road, Nanning, Guangxi Province, 530004, China., Han H; School of Biological and Food Engineering, SuZhou University of Technology, 99 South Third Ring Road, Suzhou, Jiangsu Province, 215500, China., Zhao Y; School of Biological and Food Engineering, SuZhou University of Technology, 99 South Third Ring Road, Suzhou, Jiangsu Province, 215500, China., Wang Y; School of Biological and Food Engineering, SuZhou University of Technology, 99 South Third Ring Road, Suzhou, Jiangsu Province, 215500, China., Zhang Z; School of Biological and Food Engineering, SuZhou University of Technology, 99 South Third Ring Road, Suzhou, Jiangsu Province, 215500, China., Huang W; Suzhou Kuntai Biotechnology Co., Ltd., Tower A1, Tengfei Suzhou Innovation Park, No. 388 Xinping Street, Suzhou Industrial Park, Suzhou, Jiangsu Province, 215000, China., Teng X; Suzhou Womei biology Co., Ltd., 2 Luoxiang Road, Suzhou, Jiangsu Province, 215600, China., Chen H; Suzhou Womei biology Co., Ltd., 2 Luoxiang Road, Suzhou, Jiangsu Province, 215600, China., Lu Z; Medical Enzyme Engineering Center, CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, 88 Keling Road, Suzhou, Jiangsu Province, 215004, China., Liu J; College of Light Industry and Food Engineering, Guangxi University, 100 Daxue Road, Nanning, Guangxi Province, 530004, China. Electronic address: liujd@gxu.edu.cn., Wang X; School of Biological and Food Engineering, SuZhou University of Technology, 99 South Third Ring Road, Suzhou, Jiangsu Province, 215500, China; Medical Enzyme Engineering Center, CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, 88 Keling Road, Suzhou, Jiangsu Province, 215004, China. Electronic address: wangxl@sibet.ac.cn., Zhu Y; School of Biological and Food Engineering, SuZhou University of Technology, 99 South Third Ring Road, Suzhou, Jiangsu Province, 215500, China. Electronic address: centuryrain@126.com.

Πηγή: International journal of biological macromolecules [Int J Biol Macromol] 2025 Dec; Vol. 332 (Pt 2), pp. 148703. Date of Electronic Publication: 2025 Nov 03.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Escherichia coli*/genetics , Escherichia coli*/metabolism , Carnosine*/biosynthesis , Carnosine*/chemistry , Aminopeptidases*/genetics , Aminopeptidases*/chemistry , Aminopeptidases*/metabolism , Trichoderma*/enzymology , Trichoderma*/genetics , Gene Expression*, Fermentation ; Cell-Free System ; Hydrogen-Ion Concentration ; Substrate Specificity

MEMO: A micro memo sensor detecting microRNA-RISC using an accurate cell-free expression platform.

Συγγραφείς: Copeland CE; Department of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA, 70803, USA., Heitmeier CJ; Department of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA, 70803, USA., Kim J; Department of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA, 70803, USA., Kwon YC; Department of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA, 70803, USA; Louisiana State University Agricultural Center, Baton Rouge, LA, 70803, USA; Cain Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA, 70803, USA. Electronic address: yckwon@lsu.edu.

Πηγή: Biosensors & bioelectronics [Biosens Bioelectron] 2025 Nov 01; Vol. 287, pp. 117641. Date of Electronic Publication: 2025 Jun 02.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Biosensing Techniques*/methods , Biosensing Techniques*/instrumentation , MicroRNAs*/genetics , MicroRNAs*/isolation & purification , MicroRNAs*/analysis , Micro-Electrical-Mechanical Systems*/instrumentation, Humans ; Cell-Free System ; Limit of Detection ; Equipment Design

Cell-free production of target-directed liposomes utilizing membrane localization peptides.

Συγγραφείς: Kuno K; Institute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-Star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Natsushima-cho 2-15, Yokosuka 237-0061, Japan., Shimane Y; Institute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-Star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Natsushima-cho 2-15, Yokosuka 237-0061, Japan., Sakai Y; Graduate School of Nanobioscience, Yokohama City University, Yokohama 236-0027, Japan., Kuruma Y; Institute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-Star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Natsushima-cho 2-15, Yokosuka 237-0061, Japan. Electronic address: ykuruma@jamstec.go.jp.

Πηγή: Journal of controlled release : official journal of the Controlled Release Society [J Control Release] 2025 Dec 10; Vol. 388 (Pt 1), pp. 114343. Date of Electronic Publication: 2025 Oct 18.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Elsevier Science Publishers Country of Publication: Netherlands NLM ID: 8607908 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4995 (Electronic) Linking ISSN: 01683659 NLM ISO Abbreviation: J Control Release Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Liposomes*/chemistry , Peptides*/chemistry , Peptides*/metabolism , Single-Domain Antibodies*/chemistry, Humans ; Cell-Free System ; Cell Line, Tumor

Microfluidics meets cell-free systems: from molecular engineering to synthetic cells.

Συγγραφείς: Baranwal AK; Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland., Maerkl SJ; Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. Electronic address: sebastian.maerkl@epfl.ch.

Πηγή: Current opinion in biotechnology [Curr Opin Biotechnol] 2025 Dec; Vol. 96, pp. 103384. Date of Electronic Publication: 2025 Nov 21.

Τύπος έκδοσης: Journal Article; Review

Στοιχεία περιοδικού: Publisher: Elsevier Country of Publication: England NLM ID: 9100492 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0429 (Electronic) Linking ISSN: 09581669 NLM ISO Abbreviation: Curr Opin Biotechnol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Artificial Cells* , Microfluidics*/methods , Microfluidic Analytical Techniques*/methods, Cell-Free System

Whole-cell and cell-free biosensor-driven metabolic engineering.

Συγγραφείς: Seok J; School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332-0100, United States., Styczynski MP; School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332-0100, United States. Electronic address: mark.styczynski@chbe.gatech.edu.

Πηγή: Current opinion in biotechnology [Curr Opin Biotechnol] 2025 Dec; Vol. 96, pp. 103385. Date of Electronic Publication: 2025 Nov 18.

Τύπος έκδοσης: Journal Article; Review

Στοιχεία περιοδικού: Publisher: Elsevier Country of Publication: England NLM ID: 9100492 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0429 (Electronic) Linking ISSN: 09581669 NLM ISO Abbreviation: Curr Opin Biotechnol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Biosensing Techniques*/methods , Metabolic Engineering*/methods, Cell-Free System

Controlled protein synthesis and spatial organisation in microfluidic environments.

Συγγραφείς: Gaizauskaite A; Technical University of Munich (TUM), Campus Straubing for Biotechnology and Sustainability, Germany., Crean EE; Technical University of Munich (TUM), Campus Straubing for Biotechnology and Sustainability, Germany., Banlaki I; Technical University of Munich (TUM), Campus Straubing for Biotechnology and Sustainability, Germany., Kalkowski JL; Technical University of Munich (TUM), Campus Straubing for Biotechnology and Sustainability, Germany., Niederholtmeyer H; Technical University of Munich (TUM), Campus Straubing for Biotechnology and Sustainability, Germany; SynBioFoundry@TUM, Technical University of Munich, Straubing, Germany. Electronic address: henrike.niederholtmeyer@tum.de.

Πηγή: Current opinion in biotechnology [Curr Opin Biotechnol] 2025 Dec; Vol. 96, pp. 103389. Date of Electronic Publication: 2025 Nov 14.

Τύπος έκδοσης: Journal Article; Review

Στοιχεία περιοδικού: Publisher: Elsevier Country of Publication: England NLM ID: 9100492 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0429 (Electronic) Linking ISSN: 09581669 NLM ISO Abbreviation: Curr Opin Biotechnol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Protein Biosynthesis* , Microfluidics*/methods , Microfluidic Analytical Techniques*/methods, Cell-Free System

Leveraging cell-free gene expression for RNA-based biopesticides production: mechanisms and applications.

Συγγραφείς: Sun CX; Shandong Provincial Key Laboratory of Test Technology on Food Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences, Jinan, China.; Jinan Key Laboratory of Whole-Process Quality Control of Agricultural Products, Jinan, China., Wang J; Shandong Provincial Key Laboratory of Test Technology on Food Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences, Jinan, China., Hu X; School of Resources and Environment, Shandong University of Technology, Zibo, China.

Πηγή: Pest management science [Pest Manag Sci] 2025 Dec; Vol. 81 (12), pp. 7614-7624. Date of Electronic Publication: 2025 Aug 15.

Τύπος έκδοσης: Journal Article; Review

Στοιχεία περιοδικού: Publisher: Published for SCI by Wiley Country of Publication: England NLM ID: 100898744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4998 (Electronic) Linking ISSN: 1526498X NLM ISO Abbreviation: Pest Manag Sci Subsets: MEDLINE

Ιατρικοί όροι (MeSH): RNA*/genetics , RNA*/biosynthesis , Biological Control Agents*/metabolism , Gene Expression*, Cell-Free System

Artificial cells with liquid-liquid phase separation-regulated cell-free protein synthesis.

Συγγραφείς: Fan D; School of Biomedical Engineering, Tsinghua Medicine, Tsinghua University, Beijing 100084, China., Liang K; School of Biomedical Engineering, Tsinghua Medicine, Tsinghua University, Beijing 100084, China., Wu B; School of Biomedical Engineering, Tsinghua Medicine, Tsinghua University, Beijing 100084, China., Chen MW; School of Biomedical Engineering, Tsinghua Medicine, Tsinghua University, Beijing 100084, China., Sun C; School of Biomedical Engineering, Tsinghua Medicine, Tsinghua University, Beijing 100084, China., Sun L; School of Biomedical Engineering, Tsinghua Medicine, Tsinghua University, Beijing 100084, China., Zhang Y; School of Biomedical Engineering, Tsinghua Medicine, Tsinghua University, Beijing 100084, China., Du Y; School of Biomedical Engineering, Tsinghua Medicine, Tsinghua University, Beijing 100084, China.

Πηγή: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2025 Nov 25; Vol. 122 (47), pp. e2511283122. Date of Electronic Publication: 2025 Nov 21.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Artificial Cells*/metabolism , Artificial Cells*/chemistry , Protein Biosynthesis*, Animals ; Mice ; Cell-Free System/metabolism ; Unilamellar Liposomes/chemistry ; Synthetic Biology/methods ; Phase Separation

Establishment of a cell-free multi-enzyme cascade for the synthesis of UDP-GalNAc.

Συγγραφείς: Hoang TS; Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstr. 1, 39106 Magdeburg, Germany; Otto-von-Guericke University Magdeburg, Chair of Bioprocess Engineering, Postfach 4120, 39106 Magdeburg, Germany., Wormstall ST; Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstr. 1, 39106 Magdeburg, Germany; Otto-von-Guericke University Magdeburg, Chair of Bioprocess Engineering, Postfach 4120, 39106 Magdeburg, Germany., Hoang NH; Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstr. 1, 39106 Magdeburg, Germany; eversyn GmbH, Universitätsplatz 2, Building 25, 39106 Magdeburg, Germany., Reichl U; Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstr. 1, 39106 Magdeburg, Germany; Otto-von-Guericke University Magdeburg, Chair of Bioprocess Engineering, Postfach 4120, 39106 Magdeburg, Germany., Rexer TFT; Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstr. 1, 39106 Magdeburg, Germany; eversyn GmbH, Universitätsplatz 2, Building 25, 39106 Magdeburg, Germany. Electronic address: rexer@mpi-magdeburg.mpg.de.

Πηγή: New biotechnology [N Biotechnol] 2025 Nov 25; Vol. 89, pp. 20-28. Date of Electronic Publication: 2025 May 30.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101465345 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-4347 (Electronic) Linking ISSN: 18716784 NLM ISO Abbreviation: N Biotechnol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Uridine Diphosphate N-Acetylgalactosamine*/biosynthesis, Cell-Free System

On-demand insulin manufacturing using cell-free systems with an "on-column" conversion approach.

Συγγραφείς: Borhani SG; Center for Advanced Sensor Technology, Baltimore, MD 21250, USA; Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD 21250, USA. Electronic address: borhani1@umbc.edu., Levine MZ; Department of Chemical Engineering and Department of Bioengineering, Stanford University, Stanford, CA 94305-5025, USA., Gurramkonda C; Center for Advanced Sensor Technology, Baltimore, MD 21250, USA; Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD 21250, USA., Qu Y; National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Rockville, MD 20850, USA., Tao D; National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Rockville, MD 20850, USA., LeClair CA; National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Rockville, MD 20850, USA., Swartz JR; Department of Chemical Engineering and Department of Bioengineering, Stanford University, Stanford, CA 94305-5025, USA., Rao G; Center for Advanced Sensor Technology, Baltimore, MD 21250, USA; Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD 21250, USA. Electronic address: grao@umbc.edu.

Πηγή: New biotechnology [N Biotechnol] 2025 Nov 25; Vol. 89, pp. 51-59. Date of Electronic Publication: 2025 Jun 20.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101465345 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-4347 (Electronic) Linking ISSN: 18716784 NLM ISO Abbreviation: N Biotechnol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Insulin*/biosynthesis, Cell-Free System ; Proinsulin/biosynthesis ; Humans ; Chromatography, Affinity ; Recombinant Proteins/biosynthesis

Parameterization of cell-free systems with time-series data using KETCHUP.

Συγγραφείς: Hu M; Department of Chemical Engineering, The Pennsylvania State University, University Park, Pennsylvania, United States of America., Jilani SB; Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire, United States of America., Olson DG; Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire, United States of America., Maranas CD; Department of Chemical Engineering, The Pennsylvania State University, University Park, Pennsylvania, United States of America.

Πηγή: PLoS computational biology [PLoS Comput Biol] 2025 Nov 21; Vol. 21 (11), pp. e1013724. Date of Electronic Publication: 2025 Nov 21 (Print Publication: 2025).

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238922 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7358 (Electronic) Linking ISSN: 1553734X NLM ISO Abbreviation: PLoS Comput Biol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Models, Biological* , Software*, Kinetics ; Cell-Free System/metabolism ; Computer Simulation ; Computational Biology/methods ; Formate Dehydrogenases/metabolism ; Metabolic Networks and Pathways

Cell-Free-Based Thermophilic Biocatalyst for the Synthesis of Amino Acids from One-Carbon Feedstocks.

Συγγραφείς: Westenberg R; Bioengineering Program, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.; School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, United States., Chowdhury S; School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, United States., Cardiff R; Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, Washington 98195, United States., Wennerholm K; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332, United States., Beliaev AS; Environmental Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.; Centre for Agriculture and the Bioeconomy, School of Biological and Environmental Sciences, Queensland University of Technology, Gardens Point Campus, P.O. Box 2434, Brisbane, Queensland 4001, Australia., Carothers JM; Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, Washington 98195, United States.; Department of Chemical Engineering, University of Washington, Seattle, Washington 98195, United States., Peralta-Yahya P; Bioengineering Program, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.; School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.

Πηγή: ACS synthetic biology [ACS Synth Biol] 2025 Nov 21; Vol. 14 (11), pp. 4424-4438. Date of Electronic Publication: 2025 Oct 18.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Carbon*/metabolism , Amino Acids*/biosynthesis, Escherichia coli/metabolism ; Escherichia coli/genetics ; Formates/metabolism ; Serine/biosynthesis ; Serine/metabolism ; Glycine/biosynthesis ; Biocatalysis ; Cell-Free System/metabolism ; Bicarbonates/metabolism ; Ammonia/metabolism ; Metabolic Engineering/methods

Cell-Free Transcription and Translation Tether Expressed Peptides to Their Encoding Circular DNA.

Συγγραφείς: Ichinoe K; Department of Life Science, College of Science, Rikkyo University, 3-34-1 Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan., Su'etsugu M; Department of Life Science, College of Science, Rikkyo University, 3-34-1 Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan.

Πηγή: ACS synthetic biology [ACS Synth Biol] 2025 Nov 21; Vol. 14 (11), pp. 4458-4466. Date of Electronic Publication: 2025 Oct 17.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Transcription, Genetic* , Protein Biosynthesis* , DNA, Circular*/genetics , DNA, Circular*/metabolism , Peptides*/genetics , Peptides*/metabolism, Escherichia coli/genetics ; Escherichia coli/metabolism ; Cell-Free System ; DNA-Directed RNA Polymerases/metabolism ; Plasmids/genetics ; Plasmids/metabolism ; DNA Replication

Bacterial chromosome conformation and cell-free gene expression in synthetic 2D compartments.

Συγγραφείς: Greiss F; Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, 7610001, Israel. ferdinand.greiss@gmail.com., Daube SS; Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, 7610001, Israel., Noireaux V; School of Physics and Astronomy, University of Minnesota, Minneapolis, MN, 55455, USA., Bar-Ziv R; Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, 7610001, Israel. roy.bar-ziv@weizmann.ac.il.

Πηγή: Nature communications [Nat Commun] 2025 Nov 14; Vol. 16 (1), pp. 10026. Date of Electronic Publication: 2025 Nov 14.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Chromosomes, Bacterial*/genetics , Chromosomes, Bacterial*/chemistry , Chromosomes, Bacterial*/metabolism , Escherichia coli*/genetics , Escherichia coli*/metabolism, Cell-Free System ; Transcription, Genetic ; DNA, Bacterial/genetics ; DNA, Bacterial/chemistry ; DNA, Bacterial/metabolism ; DNA-Binding Proteins/metabolism ; DNA-Binding Proteins/genetics ; Escherichia coli Proteins/metabolism ; Escherichia coli Proteins/genetics ; Adenosine Triphosphatases/metabolism ; Protein Biosynthesis ; Nucleic Acid Conformation ; Gene Expression Regulation, Bacterial ; Multiprotein Complexes/metabolism

Cryo-EM structure of a cell-free synthesized full-length human β1-adrenergic receptor in complex with G s .

Συγγραφείς: Merino F; Cube Biotech GmbH, Monheim, Germany., Köck Z; Centre for Biomolecular Magnetic Resonance, Institute for Biophysical Chemistry, Goethe University of Frankfurt/Main, Frankfurt/Main, Germany., Ermel U; Buchmann Institute for Molecular Life Sciences and Institute for Biophysics, Goethe University of Frankfurt/Main, Frankfurt/Main, Germany., Dahlhaus P; Department of Pharmaceutical Chemistry, Marburg University, Marburg, Germany., Grimm A; Centre for Biomolecular Magnetic Resonance, Institute for Biophysical Chemistry, Goethe University of Frankfurt/Main, Frankfurt/Main, Germany., Seybert A; Buchmann Institute for Molecular Life Sciences and Institute for Biophysics, Goethe University of Frankfurt/Main, Frankfurt/Main, Germany., Kubicek J; Cube Biotech GmbH, Monheim, Germany., Frangakis AS; Buchmann Institute for Molecular Life Sciences and Institute for Biophysics, Goethe University of Frankfurt/Main, Frankfurt/Main, Germany., Dötsch V; Centre for Biomolecular Magnetic Resonance, Institute for Biophysical Chemistry, Goethe University of Frankfurt/Main, Frankfurt/Main, Germany., Hilger D; Department of Pharmaceutical Chemistry, Marburg University, Marburg, Germany. Electronic address: daniel.hilger@pharmazie.uni-marburg.de., Bernhard F; Centre for Biomolecular Magnetic Resonance, Institute for Biophysical Chemistry, Goethe University of Frankfurt/Main, Frankfurt/Main, Germany. Electronic address: fbern@bpc.uni-frankfurt.de.

Πηγή: Structure (London, England : 1993) [Structure] 2025 Nov 06; Vol. 33 (11), pp. 1867-1877.e5. Date of Electronic Publication: 2025 Aug 25.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Cell Press Country of Publication: United States NLM ID: 101087697 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-4186 (Electronic) Linking ISSN: 09692126 NLM ISO Abbreviation: Structure Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Receptors, Adrenergic, beta-1*/chemistry , Receptors, Adrenergic, beta-1*/metabolism , Receptors, Adrenergic, beta-1*/genetics , GTP-Binding Protein alpha Subunits, Gs*/chemistry , GTP-Binding Protein alpha Subunits, Gs*/metabolism , GTP-Binding Protein alpha Subunits, Gs*/genetics, Humans ; Cryoelectron Microscopy ; Models, Molecular ; Protein Binding ; Cell-Free System