Investigation of new Aspergillus strains as producers of hemostatically active proteases ; Изучение спектра активности новых штаммов микромицетов рода Aspergillus в отношении белков системы гемостаза

Authors: A. A. Osmolovsky, A. A. Shestakova, D. Ye. Surkova, R. Lehotská, E. Piecková, А. А. Осмоловский, А. А. Шестакова, Д. Е. Суркова, Р. Лехотска, Е. Пецкова

Source: Vestnik Moskovskogo universiteta. Seriya 16. Biologiya; Том 78, № 2 (2023); 109-114 ; Вестник Московского университета. Серия 16. Биология; Том 78, № 2 (2023); 109-114 ; 0137-0952

Subject Terms: аспергиллы, fibrinolytic enzymes, thrombolytics, activators of human hemostatic proteins, chromogenic peptide substrates, Aspergillus, фибринолитические ферменты, тромболитические средства, активаторы белков гемостаза, хромогенные пептидные субстраты

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Relation: https://vestnik-bio-msu.elpub.ru/jour/article/view/1236/624; Shirasaka N., Naitou M., Okamura K., Fukuta Y., Terashita T., Kusuda M. Purification and characterization of a fibrinolytic protease from Aspergillus oryzae KSK-3. Mycoscience. 2012;53(5):354–364.; Zaikina N.A., Shataeva L.K., Elinov N.P., Samsonov G.V. Some properties of the protease from Aspergillus terricola. Mycopathologia. 1975;56(3):153–157.; Osmolovskiy A.A., Popova E.A., Kreyer V.G., Baranova N.A., Egorov N.S. Fibrinolytic and collagenolytic activity of extracellular proteinases of the strains of micromycetes Aspergillus ochraceus L-1 and Aspergillus ustus 1. Moscow Univ. Biol. Sci. Bull. 2016;71(1):62–66.; Kotb E.H., Gamal E.A., Edries F.M. Screening for fibrinolytic filamentous fungi and enzymatic properties of the most potent producer, Aspergillus brasiliensis AUMC 9735. Biologia. 2015:70(12):1565–1574.; Yadav S., Siddalingeshwara K. Screening and biosynthesis of fibrinolytic enzyme from Aspergillus japonicum. J. Drug Deliv. Ther. 2015;5(6):60–62.; Osmolovskiy A.A., Kreier V.G., Kurakov A.V., Baranova N.A., Egorov N.S. Aspergillus ochraceus micromycetes-producers of extracellular proteinasesprotein C activators of blood plasma. Appl. Biochem. Microbiol. 2012;48(5):488–492.; Zvonareva E.S., Osmolovskiy A.A., Kreyer V.G., Baranova N.A., Kotova I.B., Egorov N.S. Identification of targets for extracellular proteases activating proteins of the haemostatic system produced by micromycetes Aspergillus ochraceus and Aspergillus terreus. Russ. J. Bioorg. Chem. 2015;41(5):500–505.; Osmolovskiy A.A., Kurakov A.V., Kreyer V.G. Baranova N.A., Egorov N.S. Ability of extracellular proteinases of micromycetes Aspergillus flavipes, Aspergillus fumigatus, and Aspergillus sydowii to affect proteins of the human haemostatic system. Moscow Univ. Biol. Sci. Bull. 2017;72(1):20–24.; Osmolovskiy A.A., Kreyer V.G., Baranova N.A., Kurakov A.V., Egorov N.S. Production of extracellular proteinases (protein C activators in blood plasma) by the micromycete Aspergillus ochraceus during submerged and solid-state cultivation. Appl. Biochem. Microbiol. 2013;49(6):580–586.; Astrup R., Müllertz S. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 1952;40(2):346–351.; Osmolovskiy A.A., Kreier V.G., Kurakov A.V., Baranova N.A., Egorov N.S. Aspergillus ochraceus micromycetes – producers of extracellular proteinases – protein C activators of blood plasma. Appl. Biochem. Microbiol. 2013;45(5):488–492.; Šimonovičová A., Kraková L., Pangallo D., Majorošová M., Piecková E., Bodoriková S., Dörnhoferová M. Fungi on mummified human remains and in the indoor air in the Kuffner family crypt in Sládkovičovo (Slovakia). Int. Biodeter. Biodegradat. 2015;99:157–164.

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https://doi.org/10.55959/MSU0137-0952-16-78-2-7

Aspergillus proteolytic enzymes hydrolyzing fibrillar proteins for biomedicine and biotechnological process ; Протеолитические ферменты микромицетов рода Aspergillus, гидролизующие фибриллярные белки, для биомедицины и биотехнологических процессов

Authors: S. N. Timorshina, E. A. Popova, A. A. Galiakberova, A. G. Ochneva, A. A. Osmolovskiy, С. Н. Тиморшина, Е. А. Попова, А. А. Галиакберова, А. Г. Очнева, А. А. Осмоловский

Contributors: The research was funded by Russian Science Foundation, project number 22-24-00674. The studies were performed without the use of animals and without involving people as subjects. The authors declare no conflict of interest., Работа выполнена при финансовой поддержке Российского научного фонда (проект № 22-24-00674).

Source: Vestnik Moskovskogo universiteta. Seriya 16. Biologiya; Том 77, № 3 (2022); 195-200 ; Вестник Московского университета. Серия 16. Биология; Том 77, № 3 (2022); 195-200 ; 0137-0952

Subject Terms: Aspergillus, fibrillar proteins, collagenases, keratinases, fibrinolytic enzymes, elastases, фибриллярные белки, коллагеназы, кератиназы, фибринолитические ферменты, эластазы

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Relation: https://vestnik-bio-msu.elpub.ru/jour/article/view/1164/598; Yeo J., Jung G., Tarakanova A., Martín-Martínez F.J., Qin Z., Cheng Y., Zhang Y.W., Buehler M.J. Multiscale modeling of keratin, collagen, elastin and related human diseases: Perspectives from atomistic to coarse-grained molecular dynamics simulations // Extreme Mech. Lett. 2021. Vol. 20. P. 112–124.; Sweeney S.M., Orgel J.P., Fertala A., McAuliffe J.D., Turner K.R., Di Lullo G.A., Barnes A.M., Marini J.C., San Antonio J.D. Candidate cell and matrix interaction domains on the collagen fibril, the predominant protein of vertebrates // J. Biol. Chem. 2008. Vol. 283. N 30. P. 21187–21197.; Schweizer J., Bowden P.E., Coulombe P.A., Langbein L., Lane E.B., Magin T.M., Maltais L., Omary M.B., Parry D.A.D., Rogers M.A., Wright M.W. New consensus nomenclature for mammalian keratins // J. Cell Biol. 2006. Vol. 174. N 2. P. 169–174.; Libby P., Bonow R.O., Mann D.L., Tomaselli G.F., Bhatt D., Solomon S.D., Braunwald E. Braunwald’s heart disease-e-book: A textbook of cardiovascular medicine. Elsevier, 2021. 2032 pp.; Chen N.C., Srinivasan R.C., Shauver M.J., Chung K.C. A systematic review of outcomes of fasciotomy, aponeurotomy, and collagenase treatments for Dupuytren’s contracture // Hand. 2011. Vol. 6. N 3. P. 250–255.; Erdeve O., Atasay B., Arsan S. Collagenase application for amputation in a preterm // Pediatr. Dermatol. 2007. Vol. 24. N 2. P. 195–196.; Hassan M.A., Abol-Fotouh D., Omer A.M., Tamer T.M., Abbas E. Comprehensive insights into microbial keratinases and their implication in various biotechnological and industrial sectors: A review // Int. J. Biol. Macromol. 2020. Vol. 154. P. 567–583.; Kumar J., Singh I., Kushwaha R.K.S. Keratinophilic fungi: Diversity, environmental and biotechnological implications // Progress in Mycology / Eds. T. Satyanarayana, S.K. Deshmukh, and M.V. Deshpande. Singapore: Springer, 2021. P. 419–436.; Popova E.A., Kreyer V.G., Komarevtsev S.K., Shabunin S.V., Osmolovskiy A.A. Properties of extracellular proteinase of the micromycete Aspergillus ustus 1 and its high activity during fibrillary-proteins hydrolysis // Appl. Biochem. Microbiol. 2021. Vol. 57. N 2. P. 200–205.; Kate S., Pethe A. Study of collagenase production by Penicillium sp. isolated from deteriorated leather sample // J. Adv. Sci. Res. 2022. Vol. 13. N 01. P. 227–234.; Cardoso K.B.B., Nascimento M.C., Batista A.C., de Melo Oliveira V., Nascimento T.P., da Silva Batista J.M., Costa R.M.P.B., Pastrana L., Porto A.L.F. Systematic analysis on the obtaining of fibrinolytic fungi enzymes // Res., Soc. Dev. 2022. Vol. 11. N 2: e13611225449.; Звонарева Е.С., Осмоловский А.А., Крейер В.Г., Баранова Н.А., Котова И.Б., Егоров Н.С. Продукция протеиназ с плазминоподобной и активирующей прекалликреин активностью микромицетом Aspergillus terreus // Прикл. биохим. микробиол. 2018. Т. 54. № 2. С. 195–200.; Попова Е.А., Осмоловский А.А., Крейер В.Г., Котова И.Б., Егоров Н.С. Продукция штаммом Аspergillus ustus протеиназ, высокоактивных в отношении фибриллярных белков // Микол. фитопатол. 2019. Т. 53. № 4. C. 229–235.; Osmolovskiy A.A., Schmidt L., Orekhova A.V., Komarevtsev S.K., Kreyer V.G., Shabunin S.V., Egorov N.S. Action of extracellular proteases of Aspergillus flavus and Aspergillus ochraceus micromycetes on plasma hemostasis proteins // Life. 2021. Vol. 11. N 8: 782.; Osmolovskiy A.A., Schmidt L., Orekhova A.V., Kreyer V.G., Baranova N.A., Egorov N.S. Effect of proteinase from Aspergillus fumigatus on blood plasma proteins // Moscow Univ. Biol. Sci. Bull. 2021. Vol. 76. N 2. P. 71–76.; Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., Klenk D.C. Measurement of protein using bicinchoninic acid // Anal. Biochem. 1985. Vol. 150. N 1. P. 76–85.; Фокичев Н.С., Корниенко Е.И., Шаркова Т.С., Крейер В.Г., Осмоловский А.А. Тромболитическая активность и свойства препарата протеиназ, образуемых микромицетом Tolypocladium inflatum k1 // Микол. фитопатол. 2021. Т. 55. № 6. С. 449–456.; Kothary M.H., Chase T. Jr., Macmillan J.D. Correlation of elastase production by some strains of Aspergillus fumigatus with ability to cause pulmonary invasive aspergillosis in mice // Infect. Immun. 1984. Vol. 43. N 1. P. 320–325.; Tamura Y., Suzuki S., Sawada T. Role of elastase as a virulence factor in experimental Pseudomonas aeruginosa infection in mice // Microb. Pathog. 1992. Vol. 12. N 3. P. 237–244.; Khosravi A.R., Mahdavi Omran S., Shokri H., Lotfi A., Moosavi Z. Importance of elastase production in development of invasive aspergillosis // J. Mycol. Med. 2012. Vol. 22. N 2. P. 167–172.

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Evaluation of the spectrum of proteolytic activity of micromycetes of the genus Aspergillus in relation to proteins of the hemostasis system ; Оценка спектра протеолитической активности микромицетов рода Aspergillus по отношению к белкам системы гемостаза

Authors: A. A. Osmolovsky, B. Şaş, A. V. Aleksandrova, N. A. Baranova, V. G. Kreyer, А. А. Осмоловский, Б. Шаш, А. В. Александрова, Н. А. Баранова, В. Г. Крейер

Contributors: The work was supported by the Financial Council for Grants of the President of the Russian Federation No. SP-3906.2021.4., Работа выполнена при финансовой поддержке Совета по грантам Президента РФ № СП-3906.2021.4.

Source: Vestnik Moskovskogo universiteta. Seriya 16. Biologiya; Том 77, № 2 (2022); 138–144 ; Вестник Московского университета. Серия 16. Биология; Том 77, № 2 (2022); 138–144 ; 0137-0952

Subject Terms: хромогенные пептидные субстраты, fibrinolytic enzymes, thrombolytic agents, plasmin-like activity, hemostasis protein activators, chromogenic peptide substrates, фибринолитические ферменты, тромболитические средства, плазминоподобная активность, активаторы белков гемостаза

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Relation: https://vestnik-bio-msu.elpub.ru/jour/article/view/1136/591; Abdal-Aziz S.A.A., Ali S.M. Molecular characterization and differentiation of proteases isolated from different Aspergillus fungal species // Online J. Biol. Sci. 2021. Vol. 21. N 1. P. 69–119.; Galiakberova A.A., Bednenko D.M., Kreyer V.G., Osmolovskiy A.A., Egorov N.S. Formation and properties of the extracellular proteinase of Aspergillus flavus O-1 micromycete active against fibrillar proteins // Appl. Biochem. Microbiol. 2021. Vol. 57. N 5. P. 586–593.; Mustefa Beyan, S., Venkatesa Prabhu, S., Mumecha, T.K., Gemeda Mesfin T. Production of alkaline proteases using Aspergillus sp. isolated from Injera: RSM-GA based process optimization and enzyme kinetics aspect // Curr. Microbiol. 2021. Vol. 78. N 5. P. 1823–1834.; Popova E.A., Kreyer V.G., Komarevtsev S.K., Shabunin S.V., Osmolovskiy A.A. Properties of extracellular proteinase of the micromycete Aspergillus ustus 1 and its high activity during fibrillary-protein hydrolysis // Appl. Biochem. Microbiol. 2021. Vol. 57. N 2. P. 200–205.; Chung D., Yu W.-J., Lim J.-Y., Kang N.-S., Kwon Y.-M., Choi G., Bae S.-S., Cho K., Lee D.-S. Characterization of the proteolytic activity of a halophilic Aspergillus reticulatus strain SK1-1 isolated from a solar saltern // Microorganisms. 2022. Vol. 10. N 1: 29.; Diwan D., Usmani Z., Sharma M., Nelson J.W., Thakur V.K., Christie G., Molina G., Gupta V.K. Thrombolytic enzymes of microbial origin: a review // Int. J. Mol. Sci. 2021. Vol. 22. N 19: 10468.; Moula Ali A.M., Bavisetty S.C.B. Purification, physicochemical properties, and statistical optimization of fibrinolytic enzymes especially from fermented foods: a comprehensive review // Int. J. Biol. Macromol. 2020. Vol. 163. P. 1498–1517.; Осмоловский А.А., Крейер В.Г., Баранова Н.А., Егоров Н.С. Протеолитические ферменты мицелиальных грибов с плазминоподобной и активаторой к плазминогену активностью // Усп. совр. биол. 2021. Т. 141. № 5. С. 467–482.; Sharma C., Osmolovskiy A., Singh R. Microbial fibrinolytic enzymes as anti-thrombotics: production, characterization and prodigious biopharmaceutical applications // Pharmaceutics. 2021. Vol. 13. N 11: 1880.; Sharkova T.S., Kurakov A.V., Osmolovskiy A.A., Matveeva E.O., Kreyer V.G., Baranova N.A., Egorov N.S. Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes // Microbiology. 2015. Vol. 84. N 3. P. 359–364.; Fokichev N.S., Kornienko E.I., Sharkova T.S., Kreyer V.G., Osmolovskiy A.A. Thrombolytic activity and properties of the proteinase produced by the micromycete Tolypocladium inflatum k1 // Mycol. Phytopathol. 2021. Vol. 55. N 6. P. 449–456.; Kornienko E.I., Osmolovskiy A.A., Kreyer V.G., Baranova N.A., Kotova I.B., Egorov N.S. Characteristics and properties of the complex of proteolytic enzymes of the thrombolytic action of the micromycete Sarocladium strictum // Appl. Biochem. Microbiol. 2021. Vol. 57. N 1. P. 57–64.; Osmolovskiy A.A., Zvonareva E.S., Kreyer V.G., Baranova N.A., Egorov N.S. The effect of micromycete extracellular proteases of Aspergillus genus on the proteins of haemostatic system // Russ. J. Bioorg. Chem. 2014. Vol. 40. N 6. P. 634–639.; Zvonareva E.S., Osmolovskiy A.A., Kreyer V.G., Baranova N.A., Kotova I.B., Egorov N.S. Identification of targets for extracellular proteases activating proteins of the haemostatic system produced by micromycetes Aspergillus ochraceus and Aspergillus terreus // Russ. J. Bioorg. Chem. 2015. Vol. 41. N 5. P. 500–505.; Zvonareva E.S., Osmolovskiy A.A., Kreier V.G., Baranova N.A., Kotova I.B., Egorov N.S. Production of proteinase with plasmin-like and prekallikrein activating activity by the micromycete Aspergillus terreus // Appl. Biochem. Microbiol. 2018. Vol. 54. N 2. P. 206–210.; Marlar R.A., Gausman J.N. Laboratory testing issues for protein C, protein S, and antithrombin // Int. J. Lab. Hematol. 2014. Vol. 36. N 3. P. 289–295.; Osmolovskiy A.A., Kreyer V.G., Kurakov A.V., Baranova N.A., Piskunkova N.F., Egorov N.S. Micromycetes Aspergillus flavus and A. oryzae as producers of proteinases activating the proteins of human hemostasis system // Mycol. Phytopatol. 2019. Vol. 53. N 3. P. 183–185.; Lukianova A.A., Kornienko E.I., Vigand P.A., Kreyer V.G., Kurakov A.V., Osmolovskiy A.A. Secretion by micromycetes of proteases with activity similar to the activity of the hemostatic system proteins // Moscow Univ. Biol. Sci. Bull. 2020. Vol. 75. N 1. P. 31–35.; Osmolovskiy A.A., Rukavitsyna E.D., Kreier V.G., Baranova N.A., Egorov N.S. Production of proteinases with fibrinolytic and fibrinogenolytic activity by a micromycete Aspergillus ochraceus // Microbiology. 2017. Vol. 86. N 4. P. 512–516.; Batomunkueva B.P., Egorov N.S. Isolation, purification and resolution of the extracellular proteinase complex of Aspergillus ochraceus 513 with fibrinolytic and anticoagulant activities // Microbiology. 2001. Vol. 70. N 5. P. 519–522.; Proteolytic Enzymes. A practical approach / Eds. R. Beynon and J.S. Bond. Oxford: Oxford Univ. Press, 2001. 340 pp.; Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding // Anal. Biochem. 1976. Vol. 72. N 1–2. P. 248–254.

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Secretion of proteinase with activity that is similar to activity of proteins of the hemostatic system by micromycetes ; Секреция микромицетами протеиназ с активностью, подобной активности белков системы гемостаза

Authors: A. A. Lukianova, E. I. Kornienko, P. A. Vigand, V. G. Kreyer, A. V. Kurakov, A. A. Osmolovskiy, А. А. Лукьянова, Е. И. Корниенко, П. А. Виган, В. Г. Крейер, А. В. Кураков, А. А. Осмоловский

Source: Vestnik Moskovskogo universiteta. Seriya 16. Biologiya; Том 75, № 1 (2020); 37-42 ; Вестник Московского университета. Серия 16. Биология; Том 75, № 1 (2020); 37-42 ; 0137-0952

Subject Terms: хромогенные пептидные субстраты, fibrinolytic enzymes, thrombolytic agents, plasmin-like activity, plasminogen activators, chromogenic peptide substrates, фибринолитические ферменты, тромболитические средства, плазминоподобная активность, активаторы плазминогена

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Vol. 72. N 1. P. 20–24.; Шаркова Т.С., Матвеева Э.О., Кураков А.В., Осмоловский А.А., Крейер В.Г., Баранова Н.А., Егоров Н.С. Энтомопатогенные микромицеты – перспективные продуценты протеиназ с фибринолитической активностью // Усп. мед. микол. 2015. Т. 14. С. 458–460.; Kim H.C., Choi B.-S., Sapkota K., Kim S., Lee H.J., Yoo J.C., Kim S.-J. Purification and characterization of a novel, highly potent fibrinolytic enzyme from Paecilomyces tenuipes // Process Biochem. 2011. Vol. 46. N 8. P. 1545–1553.; Osmolovskiy A.A., Kreyer V.G., Baranova N.A., Egorov N.S. Properties of extracellular plasmin-like proteases of Aspergillus ochraceus micromycete // Applied Biochem. Microbiol. 2017. Vol. 53. N 4. P. 429–434.; Batomunkueva B.P., Egorov N.S. Isolation, purification and resolution of the extracellular proteinase complex of Aspergillus ochraceus 513 with fibrinolytic and anticoagulant activities // Microbiology. 2001. Vol. 70. N 5. P. 519–522.; Osmolovskiy A.A., Rukavitsyna E.D., Kreier V.G., Baranova N.A., Egorov N.S. Production of proteinases with fibrinolytic and fibrinogenolytic activity by a micromycete Aspergillus ochraceus // Microbiology. 2017. Vol. 86. N 4. P. 512–516.; Proteolytic Enzymes. A practical approach / Ed. R. Beynon and J.S. Bond. Oxford: Oxford Univ. Press, 2001. 340 pp.; Егоров Н.С., Ландау Н.С., Буяк Л.И., Крейер В.Г. Гидролитическая система нокардиоформной бактерии Nocardia minima в процессе ее роста, развития и дифференциации // Микробиология. 1991. Т. 60. N 4. С. 637–643.; Osmolovskiy A.A., Popova E.A., Kreyer V.G., Baranova N.A., Egorov N.S. Fibrinolytic and collagenolytic activity of extracellular proteinases of the strains of micromycetes Aspergillus ochraceus L-1 and Aspergillus ustus 1 // Moscow Univ. Biol. Sci. Bull. 2016. Vol. 71. N 1. P. 62–66.; Bradford M.M. 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SECRETION OF PROTEINASES WITH FIBRINOLYTIC ACTIVITY BY MICROMYCETES OF THE GENUS ASPERGILLUS ; СЕКРЕЦИЯ ПРОТЕИНАЗ С ФИБРИНОЛИТИЧЕСКОЙ АКТИВНОСТЬЮ МИКРОМИЦЕТАМИ РОДА ASPERGILLUS

Authors: A. A. Osmolovskiy, E. S. Zvonareva, V. G. Kreyer, N. A. Baranova, N. S. Egorov, А. А. Осмоловский, Е. С. Звонарева, В. Г. Крейер, Н. А. Баранова, Н. С. Егоров

Source: Vestnik Moskovskogo universiteta. Seriya 16. Biologiya; Том 73, № 1 (2018); 47-51 ; Вестник Московского университета. Серия 16. Биология; Том 73, № 1 (2018); 47-51 ; 0137-0952

Subject Terms: источники азота, Aspergillus, fibrinolytic enzymes, thrombolytic agents, plasmin-like activity, nitrogen sources, аспергиллы, фибринолитические ферменты, тромболитические средства, плазминоподобная активность

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Relation: https://vestnik-bio-msu.elpub.ru/jour/article/view/527/420; Kotb E. The biotechnological potential of fibrinolytic enzymes in the dissolution of endogenous blood thrombi // Biotechnol. Prog. 2014. Vol. 30. N 3. P. 656–672.; Sharkova T.S., Kurakov A.V., Osmolovskiy A.A., Matveeva E.O., Kreyer V.G., Baranova N.A., Egorov N.S. Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes // Microbiology. 2015. Vol. 84. N 3. P. 359–364.; Balami J.S., Chen R., Sutherland B.A., Buchan A.M. Thrombolytic agents for acute ischaemic stroke treatment: the past, present and future // CNS Neurol. Disord. Drug Targets. 2013. Vol. 12. N 2. P. 145–154.; Batomunkueva B.P., Egorov N.S. Isolation, purification and resolution of the extracellular proteinase complex of Aspergillus ochraceus 513 with fibrinolytic and anticoagulant activities // Microbiology. 2001. Vol. 70. N 5. P. 519–522.; Kotb E., Helal G.E.-D.A., Edries F.M. Screening for fibrinolytic filamentous fungi and enzymatic properties of the most potent producer, Aspergillus brasiliensis AUMC 9735 // Biologia. 2015. Vol. 70. N 12. P. 1565–1574.; Aradhye P.K., Chavan M.D. Production and characterization of fibrinolytic enzyme from Aspergillus niger // World J. Pharm. Pharm. Sci. 2015. Vol. 3. N 9. P. 843–851.; Yadav S., Siddalingeshwara K.G. Screening and biosynthesis of fibrinolytic enzyme from Aspergillus japonicum // J. Drug Deliv. Therap. 2015. Vol. 5. N 6. P. 60–62.; Osmolovskiy A.A., Popova E.A., Kreyer V.G., Baranova N.A., Egorov N.S. Fibrinolytic and collagenolytic activity of extracellular proteinases of the strains of micromycetes Aspergillus ochraceus L-1 and Aspergillus ustus 1 // Moscow Univ. Biol. Sci. Bull. 2016. Vol. 71. N 1. P. 62–66.; Osmolovskiy A.A., Rukavitsyna E.D., Kreier V.G., Baranova N.A., Egorov N.S. Production of proteinases with fibrinolytic and fibrinogenolytic activity by a micromycete Aspergillus ochraceus // Microbiology. 2017. Vol. 86. N 4. P. 512–516.; Gupta P., Samant K. Sahu A. Isolation of cellulosedegrading bacteria and determination of their cellulolytic potential // Int. J. Microbiol. 2012. Vol. 22. Article ID 578925.; Behera B.C., Parida S., Dutta S.K., Thatoi H.N. Isolation and identification of cellulose degrading bacteria from angrove soil of Mahanadi river delta and their cellulose production ability // Am. J. Mic robiol. Res. 2014. Vol. 2. N 1. P. 41–46.; Егоров Н.С., Ландау Н.С., Буяк Л.И., Крейер В.Г. Гидролитическая система нокардиоформной бактерии Nocardia minima в процессе ее роста, развития и дифференциации // Микробиология. 1991. Т. 60. № 4. С. 637–643.; Nirmal N.P., Shankar S., Laxman R.S. Fungal proteases: an overview // Int. J. Biotech. Biosci. 2011. Vol. 1. N 1. P. 1–40.; El-Aassar S.A., El-Badry H.M., Abdel-Fattah A.F. The biosynthesis of proteases with fibrinolytic activity in immobilized cultures of Penicillium chrysogenum H9 // Appl. Microbiol. Biotechnol. 1990. Vol. 33. N 1. P. 26–30.; Zvonareva E.S., Osmolovskiy A.A., Kreyer V.G., Baranova N.A., Kotova I.B., Egorov N.S. Identification of targets for extracellular proteases activating proteins of the haemostatic system produced by micromycetes Aspergillus ochraceus and Aspergillus terreus // Russ. J. Bioorg. Chem. 2015. Vol. 41. N 5. P. 500–505.; Osmolovskiy A.A., Kreyer V.G., Baranova N.A., Egorov N.S. Properties of extracellular plasmin-like proteases of Aspergillus ochraceus micromycete // Applied Biochem. Microbiol. 2017. Vol. 53. N 4. P. 429–434.

Availability: https://vestnik-bio-msu.elpub.ru/jour/article/view/527

SECRETION OF EXTRACELLULAR PROTEINASES, ACTIVE AGAINST FIBRILLARY PROTEINS, BY MICROMYCETES ; СЕКРЕЦИЯ МИКРОМИЦЕТАМИ ВНЕКЛЕТОЧНЫХ ПРОТЕИНАЗ, АКТИВНЫХ ПО ОТНОШЕНИЮ К ФИБРИЛЛЯРНЫМ БЕЛКАМ

Authors: E. A. Popova, D. M. Bednenko, A. A. Osmolovskiy, V. G. Kreyer, I. B. Kotova, N. S. Egorov, Е. А. Попова, Д. М. Бедненко, А. А. Осмоловский, В. Г. Крейер, И. Б. Котова, Н. С. Егоров

Source: Vestnik Moskovskogo universiteta. Seriya 16. Biologiya; Том 72, № 4 (2017); 241-245 ; Вестник Московского университета. Серия 16. Биология; Том 72, № 4 (2017); 241-245 ; 0137-0952

Subject Terms: энзиматический индекс, proteolytic activity, fibrinolytic enzymes collagenolytic enzymes, elastolytic enzymes, enzymatic index, протеолитическая активность, фибринолитические ферменты, коллагенолитические ферменты, эластолитические ферменты

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Relation: https://vestnik-bio-msu.elpub.ru/jour/article/view/495/408; Wanderley M.C. de A., Neto J.M.W.D., Filho J.L. de L., Lima C. de A., Teixeira J.A.C., Porto A.L.F. Collagenolytic enzymes produced by fungi: a systematic review // Braz. J. Microbiol. 2017. Vol. 48. N 1. P. 13–24.; Kotb E. The biotechnological potential of fibrinolytic enzymes in the dissolution of endogenous blood thrombi // Biotechnol. Prog. 2014. Vol. 30. N 3. P. 656–672.; Sharkova T.S., Kurakov A.V., Osmolovskiy A.A., Matveeva E.O., Kreyer V.G., Baranova N.A., Egorov N.S. Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes // Microbiology. 2015. Vol. 84. N 3. P. 359–364.; Rudenskaya G.N. Brachyurins, serine collagenolytic enzymes from crabs // Russ. J. Bioorg. Chem. 2003. Vol. 29. N 2. P. 101–111.; Watanabe K. Collagenolytic proteases from bacteria // Appl. Microbiol. Biotechnol. 2004. Vol. 63. N 5. P. 520–526.; Hasan S., Ahmad A., Purwar A., Khan N., Kundan R., Gupta G. Enzymes in the entomopathogenic fungus Verticillium lecanii // Bioinformation. 2013. Vol. 9. N 5. P. 238–242.; Yike I. Fungal proteases and their pathophysiological effects // Mycopathologia. 2011. Vol. 171. N 5. P. 299–323.; Osmolovskiy A.A., Rukavitsyna E.D., Kreier V.G., Baranova N.A., Egorov N.S. Production of proteinases with fibrinolytic and fibrinogenolytic activity by a micromycete Aspergillus ochraceus // Microbiology. 2017. Vol. 86. N 4. P. 512–516.; Gupta P., Samant K. Sahu A. Isolation of cellulosedegrading bacteria and determination of their cellullytic potential // Int. J. Microbiol. 2012. Vol. 22. Article ID 578925.; Behera B.C., Parida S., Dutta S.K., Thatoi N.H. Isolation and identification of cellulose degrading bacteria from angrove soil of Mahanadi river delta and their cellulose production ability // Am. J. Microbiol. Res. 2014. Vol. 2. N 1. P. 41–46.; Osmolovskiy A.A., Popova E.A., Kreyer V.G., Baranova N.A., Egorov N.S. Fibrinolytic and collagenolytic activity of extracellular proteinases of the strains of micromycetes Aspergillus ochraceus L-1 and Aspergillus ustus 1 // Moscow Univ. Biol. Sci. Bull. 2016. Vol. 71. N 1. P. 62–66.; Chavira R. Jr., Burnett T.J., Hageman J.N. Assaying proteinase with Azocoll // Anal. Biochem. 1984. Vol. 136. N 2. P. 446–450.; El-Aassar S.A., El-Badry H.M., Abdel-Fattah A.F. The biosynthesis of proteases with fibrinolytic activity in immobilized cultures of Penicillium chrysogenum H9 // Appl. Microbiol. Biotechnol. 1990. Vol. 33. N 1. P. 26–30.; Osmolovskiy A.A., Kurakov A.V., Kreyer V.G., Baranova N.A., Egorov N.S. Ability of extracellular proteinases of micromycetes Aspergillus flavipes, Aspergillus fumigatus, and Aspergillus sydowii to affect proteins of the human haemostatic system // Moscow Univ. Biol. Sci. Bull. 2017. Vol. 72. N 1. P. 20–24.; Lima C.A., Viana Marques D.A., Neto B.B., Lima Filho J.L., Carneiro-da-Cunha M.G., Porto A.L.F. Fermentation medium for collagenase production by Penicillium aurantiogriseum URM4622 // Biotechnol. Prog. 2011. Vol. 27. N 5. P. 1470–1477.; Егоров Н.С., Ландау Н.С., Буяк Л.И., Крейер В.Г. Гидролитическая система нокардиоформной бактерии Nocardia minima в процессе ее роста, развития и дифференциации // Микробиология. 1991. Т. 60. № 4. С. 637–643.

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