Influence of Bacterial Encapsulation on Urease Activity During Microbially Induced Calcite Precipitation

Authors: Joshi, Renu^Aff15, Bhukya, Pavan Kumar^Aff15, Arnepalli, Dali Naidu^Aff15, Maji, Vidya Bhushan^Aff15

Contributors: di Prisco, Marco, Series Editor^Aff1, Chen, Sheng-Hong, Series Editor^Aff2, Vayas, Ioannis, Series Editor^Aff3, Kumar Shukla, Sanjay, Series Editor^Aff4, Sharma, Anuj, Series Editor^Aff5, Kumar, Nagesh, Series Editor^Aff6, Wang, Chien Ming, Series Editor^Aff7, Cui, Zhen-Dong, Series Editor^Aff8, Lu, Xinzheng, Series Editor^Aff9, Cameselle, Claudio, editor^Aff10, Gouveia, Susana, editor^Aff11, Urréjola, Santiago, editor^Aff12, Reddy, Krishna R., editor^Aff13, Agnihotri, Arvind Kumar, editor^Aff14

Source: Sustainable Environmental Geotechnology and Pollution Control : Select Proceedings of EGRWSE 2025, Volume 2. 804:151-162

Activity and stability of urease enzyme molecules for on-site urea measurement in milk

Additional Titles: The nitrogenous metabolite urea is an important biomarker that indicates cows’ nutritional intake. Today, determining the urea concentration from milk samples requires analysis techniques in a lab which is time-consuming and expensive. It would be favourable to have an on-site measurement method to achieve a fast detection allowing farmers to quickly adjust the feed of individual cows, which might make it possible to reduce costs, increase milk production, and/or reduce the amount of nitrogen emission to the environment. This thesis is a part of a collaboration project between Linköping University and the Swedish University of Agricultural Sciences named “On-farm measurement of milk urea - development of a sensor”. The thesis investigated the activity and stability of the urease in an electrochemical biosensor, in which the urease is immobilised via encapsulation in a gel of poly(carbamoyl sulfonate) on a screenprinted electrode coated with a metal catalyst (copper), a cation exchanger (Nafion) and a conductive polymer (polyaniline). The linear range of the biosensor was successfully extended up to 2500 μM urea with a diffusion barrier composed of chitosan and polyvinyl butyral, enabling higher urea concentrations measurement than without the barrier (680 μM). Reproducibility, reusability, and storage stability measurements of the urease immobilised electrodes were performed and evaluated. A comparison between free enzyme, immobilised enzyme on the electrode surface and immobilised enzyme on an electrode surface that had been stored for a few days, was conducted to determine urease activity and stability. In addition, five components of milk (casein, lactose, ammonium, iron, and ascorbic acid) were measured separately to evaluate their interferences during milk urea detection. Interference was observed in several cases. A final evaluation of the present electrochemical biosensor was done by analysis of real milk samples giving promising results for future developmen

Authors: Valtersson, Emma

Index Terms: Kemisk biologi, urea i mjölk, biosensor, Analytical Chemistry, Analytisk kemi, Student thesis, info:eu-repo/semantics/bachelorThesis, text

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-190544

Urease immobilized core–shell magnetic Fe[NiFe]O4/alginate and Fe3O4/alginate composite beads with improved enzymatic stability properties: removal of artificial blood serum urea

Authors: Yasemin İspirli Doğaç, Mustafa Teke

Contributors: MÜ, Muğla Meslek Yüksekokulu, Kimya ve Kimyasal İşleme Teknolojileri Bölümü, İspirli Doğaç, Yasemin, Teke, Mustafa

Source: Journal of the Iranian Chemical Society. 18:2637-2648

Subject Terms: Urease encapsulation, 0106 biological sciences, 0301 basic medicine, 03 medical and health sciences, Fe[NiFe]O4, Alginate, Fe3O4, Nanoparticles, 01 natural sciences, Magnetic polymeric beads

File Description: application/pdf

Chitosan based core–shell microgel support for urease: Step up of enzyme activity, stability and storage

Authors: Chaudhary, Aradhana ^{a, f}, Pande, P.P. ^{a, f}, Kumar, Krishna ^{b, f, ⁎}, Prasad, Tarkeshwar ^{a, f}, Rai, Shailja ^{a, f}, Singh, Vinai K. ^{c, f}, Tungala, Kranthikumar ^{d, f}, Kumar, Dhananjay ^{e, f}, Dutta, Arunava ^{e, f}

Source: In Next Materials April 2025 7

Reinforcement of urease through encapsulation with β-Cyclodextrin-based bioconjugates: A comparative analysis and kinetic assessment

Authors: Rai, Shailja ^a, Pande, Poorn Prakash ^a, Kumar, Krishna ^{b, ⁎}, Tungala, Kranthikumar ^c, Tiwari, Rudramani ^d, Parwati, Km ^e, Krishnamoorthi, S. ^e, Chaudhary, Aradhana ^a, Dutta, Arunava ^f, Prasad, Tarkeshwar ^a, Singh, Vinai Kumar ^g

Source: In Reactive and Functional Polymers January 2026 218

Urease Encapsulation in Nanoorganized Microshells

Authors: Lvov, Y., Antipov, A., Mamedov, A., Möhwald, H., Sukhorukov, G.

Source: Nano Letters

Subject Terms: 02 engineering and technology, 0210 nano-technology, 01 natural sciences, 6. Clean water, 3. Good health, 0104 chemical sciences

File Description: application/pdf

Access URL: https://hdl.handle.net/11858/00-001M-0000-0015-6869-C
https://hdl.handle.net/11858/00-001M-0000-0015-686B-8

Co-encapsulation of Au nanoclusters and carbon dots in ZIF with reversed acid-responsiveness for enhanced point-of-care testing of urease activity

Authors: Wang, Shun ^a, Li, Lei ^a, Sun, Guoming ^b, Wu, Zhina ^{c, ⁎}, Bai, Qian ^{a, ⁎⁎}, Wang, Mengke ^{b, ⁎⁎⁎}

Source: In Analytica Chimica Acta 15 January 2026 1383

Co-encapsulated urease and glucose oxidase in polyelectrolyte microcapsules for simultaneous glucose and urea determination

Authors: Kim, Aleksandr L. ^⁎, Musin, Egor V., Tikhonenko, Sergey A.

Source: In Analytica Chimica Acta 15 October 2026 1419

Biopolymer matrix for nano-encapsulation of urease – A model protein and its application in urea detection

Authors: Saxena, Abhishek ^a, Bhattacharya, Arpita ^b, Kumar, Satish ^c, Epstein, Irving R. ^d, Sahney, Rachana ^{a, ⁎}

Source: In Journal of Colloid And Interface Science 15 March 2017 490:452-461

Encapsulation of Dicranopteris linearis extract using cellulose microparticles for antiulcer medication

Authors: Ahmad, Anis Adilah ^a, Kasim, Khairul Farihan ^{a, b, ⁎}, Gopinath, Subash C.B. ^{a, c, d, e}, Anbu, Periasamy ^f, Sofian-Seng, Noor-Soffalina ^g

Source: In International Journal of Biological Macromolecules 31 December 2023 253 Part 2

Edible packaging fucoidan/ovalbumin encapsulation co-delivery of indigo and Indirubin nanoparticles: Preparation, characterization, and preliminary mechanism of action against Helicobacter pylori in vitro

Authors: Lin, Fengli ^{a, 1}, Chen, Meiyun ^{b, 1}, Li, Yanqi ^a, Li, Haonan ^c, Cui, Jie ^c, Shi, Xiaoyan ^d, Xiao, He ^e, Jia, Junwei ^d, Wei, Ruixia ^e, Zhang, Guimin ^{d, ⁎}, Yao, Meicun ^{f, ⁎⁎}, Feng, Zhong ^{a, c, e, ⁎⁎⁎}

Source: In International Journal of Biological Macromolecules February 2026 341 Part 1

Dual-protection mechanism in urease-immobilized calcium alginate- vaterite composite gel for enhanced Cd2+ removal from wastewater: Insights into the process

Authors: Jia, Hui ^{a, b}, Sang, Jingxuan ^a, Jin, Bingbing ^a, Wang, Shengqiang ^{a, c}, Hou, Qianqian ^a, Wu, Yifan ^a, Dapaah, Malcom Frimpong ^a, Shi, Peili ^{d, ⁎}, Cheng, Liang ^{a, b, ⁎⁎}

Source: In Process Safety and Environmental Protection December 2025 204

Sodium alginate-based hydrogel combined with MOF-dynamic defect generation strategy: A nanobiocomposite for synergistic enhancement of urease immobilization

Authors: Wang, Zimeng ^a, Wang, Biao ^a, Yang, Leilei ^a, Guo, Xiaofang ^a, Liu, Zhuang ^a, Chai, Yamin ^b, Du, Yunzheng ^a, Tian, Ziying ^a, Yang, Jingxuan ^a, Zhu, Chunling ^a, Cao, Xuesen ^{c, ⁎}, Ou, Lailiang ^{a, ⁎}

Source: In International Journal of Biological Macromolecules January 2026 338 Part 1

Development of a bienzymatic diagnostic system based on separately encapsulated urease and glucose oxidase for the simultaneous determination of urea and glucose in a single analytical sample

Authors: Kim, Aleksandr L. ^⁎, Musin, Egor V., Tikhonenko, Sergey A. ^⁎

Source: In Colloids and Surfaces A: Physicochemical and Engineering Aspects 20 August 2025 719

Enhancing hexavalent chromium stable reduction via sodium alginate encapsulation of newly isolated fungal and bacterial consortia

Authors: Zveushe, Obey Kudakwashe ^a, Nkoh, Jackson Nkoh ^b, de Dios, Víctor Resco ^{c, d}, Manjoro, Tendai Terence ^e, Suanon, Fidèle ^a, Zhang, Hengxing ^a, Chen, Wenfang ^a, Lin, Lin ^a, Zhou, Lei ^f, Zhang, Wei ^g, Sesu, Frank ^a, Li, Jin ^a, Han, Ying ^{a, ⁎}, Dong, Faqin ^{e, h, ⁎⁎}

Source: In Journal of Hazardous Materials 15 March 2025 486

Poly(methylene co-guanidine) coated alginate as an encapsulation matrix for urease

Authors: Hearn, Elizabeth, Neufeld, Ronald J ^*

Source: In Process Biochemistry 2000 35(10):1253-1260

Emergence of ADM-mediated bioconjugate to enhance longevity and catalytic efficiency of urease

Authors: Rai, Shailja ^a, Pande, Poorn Prakash ^a, Kumar, Krishna ^{b, ⁎}, Chaudhary, Aradhana ^a, Prasad, Tarkeshwar ^a, Tiwari, Rudramani ^c, Parwati, Km ^d, Krishnamoorthi, S. ^d, Dutta, Arunava ^e

Source: In International Journal of Biological Macromolecules March 2025 296

2-Amino-4-chlorophenol based Schiff base allied organosilatrane and its silica nanoparticles: Sensing of Cu(II) ion and urease inhibitory activity via spectroscopic and molecular docking study

Authors: Singh, Gurjaspreet ^{a, ⁎}, Kaur, Jashan Deep ^{b, ⁎⁎}, Pawan ^c, Tamana ^a, Khurana, Sumesh ^a, Stanzin, Jigmat ^a, Rani, Bhavana ^a, Diskit, Tsering ^a, Singh, Kamal Nain ^a

Source: In Journal of Molecular Structure 15 January 2025 1320

Coupled influence of biochemical factors on the precipitated calcite crystal characteristics in microbially induced calcite precipitation

Authors: Joshi, Renu, Bhukya, Pavan Kumar, Arnepalli, Dali Naidu ^⁎

Source: In Journal of Rock Mechanics and Geotechnical Engineering July 2026 18(7):5608-5627

Synthesis and Characterisation of Biocompatible Polymer-Conjugated Magnetic Beads for Enhancement Stability of Urease

Authors: Doğaç, Yasemin İspirli, Teke, Mustafa

Contributors: MÜ, Fen Fakültesi, Kimya Bölümü, Doğaç, Yasemin İspirli, Teke, Mustafa

Source: Applied Biochemistry and Biotechnology. 179:94-110

Subject Terms: Urease Encapsulation, 0106 biological sciences, 0301 basic medicine, Chitosan, Magnetic Phenomena, Temperature, Enzymes, Immobilized, Urease, 01 natural sciences, Kinetics, 03 medical and health sciences, Biopolymers, Enzyme Stability, Fe[Nife]O-4 Nanoparticles, Urea, Fe3O4 Nanoparticles, Urease Stability, Magnetic Polymeric Beads

File Description: application/pdf

Access URL: https://pubmed.ncbi.nlm.nih.gov/26758712