Reinforcement of urease through encapsulation with β-Cyclodextrin-based bioconjugates: A comparative analysis and kinetic assessment

Authors: Rai, Shailja ^a, Pande, Poorn Prakash ^a, Kumar, Krishna ^{b, ⁎}, Tungala, Kranthikumar ^c, Tiwari, Rudramani ^d, Parwati, Km ^e, Krishnamoorthi, S. ^e, Chaudhary, Aradhana ^a, Dutta, Arunava ^f, Prasad, Tarkeshwar ^a, Singh, Vinai Kumar ^g

Source: In Reactive and Functional Polymers January 2026 218

Co-encapsulation of Au nanoclusters and carbon dots in ZIF with reversed acid-responsiveness for enhanced point-of-care testing of urease activity

Authors: Wang, Shun ^a, Li, Lei ^a, Sun, Guoming ^b, Wu, Zhina ^{c, ⁎}, Bai, Qian ^{a, ⁎⁎}, Wang, Mengke ^{b, ⁎⁎⁎}

Source: In Analytica Chimica Acta 15 January 2026 1383

Co-encapsulated urease and glucose oxidase in polyelectrolyte microcapsules for simultaneous glucose and urea determination

Authors: Kim, Aleksandr L. ^⁎, Musin, Egor V., Tikhonenko, Sergey A.

Source: In Analytica Chimica Acta 15 October 2026 1419

Influence of Bacterial Encapsulation on Urease Activity During Microbially Induced Calcite Precipitation

Authors: Joshi, Renu^Aff15, Bhukya, Pavan Kumar^Aff15, Arnepalli, Dali Naidu^Aff15, Maji, Vidya Bhushan^Aff15

Contributors: di Prisco, Marco, Series Editor^Aff1, Chen, Sheng-Hong, Series Editor^Aff2, Vayas, Ioannis, Series Editor^Aff3, Kumar Shukla, Sanjay, Series Editor^Aff4, Sharma, Anuj, Series Editor^Aff5, Kumar, Nagesh, Series Editor^Aff6, Wang, Chien Ming, Series Editor^Aff7, Cui, Zhen-Dong, Series Editor^Aff8, Lu, Xinzheng, Series Editor^Aff9, Cameselle, Claudio, editor^Aff10, Gouveia, Susana, editor^Aff11, Urréjola, Santiago, editor^Aff12, Reddy, Krishna R., editor^Aff13, Agnihotri, Arvind Kumar, editor^Aff14

Source: Sustainable Environmental Geotechnology and Pollution Control : Select Proceedings of EGRWSE 2025, Volume 2. 804:151-162

Biopolymer matrix for nano-encapsulation of urease – A model protein and its application in urea detection

Authors: Saxena, Abhishek ^a, Bhattacharya, Arpita ^b, Kumar, Satish ^c, Epstein, Irving R. ^d, Sahney, Rachana ^{a, ⁎}

Source: In Journal of Colloid And Interface Science 15 March 2017 490:452-461

Synthesis and characterization of a stable humic–urease complex: application to barley seed encapsulation for improving N uptake

Authors: Mvila, Beaufray Gilaime, Pilar Izquierdo, María Concepción, Busto Núñez, Mª Dolores, Pérez Mateos, Manuel, Ortega Santamaría, Natividad

Source: Repositorio Institucional de la Universidad de Burgos (RIUBU)
Institut de Recerca Germans Trias i Pujol (IGTP)

Subject Terms: Bioquímica, Models, Molecular, Alginates, Germination, Nitrogen availability, Biochemistry, Seed encapsulation, Glucuronic Acid, Ammonium Compounds, Enzyme Stability, Enzyme immobilization, Fertilizers, Seed coating, Humic Substances, Plant growth, Plant Proteins, 2. Zero hunger, Agricultura, Hexuronic Acids, 0402 animal and dairy science, Flocculation, Agriculture, Hordeum, Soil urease, 04 agricultural and veterinary sciences, 15. Life on land, Nitrogen Cycle, Enzymes, Immobilized, Crop Production, Canavalia, Seedlings, Seeds, 0401 agriculture, forestry, and fisheries, Calcium, 0405 other agricultural sciences, Gels

File Description: application/pdf

Access URL: https://pubmed.ncbi.nlm.nih.gov/26381854
http://hdl.handle.net/10259/8264

Encapsulation of Dicranopteris linearis extract using cellulose microparticles for antiulcer medication

Authors: Ahmad, Anis Adilah ^a, Kasim, Khairul Farihan ^{a, b, ⁎}, Gopinath, Subash C.B. ^{a, c, d, e}, Anbu, Periasamy ^f, Sofian-Seng, Noor-Soffalina ^g

Source: In International Journal of Biological Macromolecules 31 December 2023 253 Part 2

Activity and stability of urease enzyme molecules for on-site urea measurement in milk

Additional Titles: The nitrogenous metabolite urea is an important biomarker that indicates cows’ nutritional intake. Today, determining the urea concentration from milk samples requires analysis techniques in a lab which is time-consuming and expensive. It would be favourable to have an on-site measurement method to achieve a fast detection allowing farmers to quickly adjust the feed of individual cows, which might make it possible to reduce costs, increase milk production, and/or reduce the amount of nitrogen emission to the environment. This thesis is a part of a collaboration project between Linköping University and the Swedish University of Agricultural Sciences named “On-farm measurement of milk urea - development of a sensor”. The thesis investigated the activity and stability of the urease in an electrochemical biosensor, in which the urease is immobilised via encapsulation in a gel of poly(carbamoyl sulfonate) on a screenprinted electrode coated with a metal catalyst (copper), a cation exchanger (Nafion) and a conductive polymer (polyaniline). The linear range of the biosensor was successfully extended up to 2500 μM urea with a diffusion barrier composed of chitosan and polyvinyl butyral, enabling higher urea concentrations measurement than without the barrier (680 μM). Reproducibility, reusability, and storage stability measurements of the urease immobilised electrodes were performed and evaluated. A comparison between free enzyme, immobilised enzyme on the electrode surface and immobilised enzyme on an electrode surface that had been stored for a few days, was conducted to determine urease activity and stability. In addition, five components of milk (casein, lactose, ammonium, iron, and ascorbic acid) were measured separately to evaluate their interferences during milk urea detection. Interference was observed in several cases. A final evaluation of the present electrochemical biosensor was done by analysis of real milk samples giving promising results for future developmen

Authors: Valtersson, Emma

Index Terms: Kemisk biologi, urea i mjölk, biosensor, Analytical Chemistry, Analytisk kemi, Student thesis, info:eu-repo/semantics/bachelorThesis, text

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-190544

Edible packaging fucoidan/ovalbumin encapsulation co-delivery of indigo and Indirubin nanoparticles: Preparation, characterization, and preliminary mechanism of action against Helicobacter pylori in vitro

Authors: Lin, Fengli ^{a, 1}, Chen, Meiyun ^{b, 1}, Li, Yanqi ^a, Li, Haonan ^c, Cui, Jie ^c, Shi, Xiaoyan ^d, Xiao, He ^e, Jia, Junwei ^d, Wei, Ruixia ^e, Zhang, Guimin ^{d, ⁎}, Yao, Meicun ^{f, ⁎⁎}, Feng, Zhong ^{a, c, e, ⁎⁎⁎}

Source: In International Journal of Biological Macromolecules February 2026 341 Part 1

Alginate Beads Encapsulation Matrix for Urease and Polyethyleneglycol-Urease

Authors: Baysal, Senay Hamarat

Contributors: Ege University Institutional Repository

Source: Artificial Cells, Blood Substitutes, and Biotechnology. 35:457-465

Subject Terms: 0106 biological sciences, Alginates, Capsules, In Vitro Techniques, 01 natural sciences, Polyethylene Glycols, Drug Delivery Systems, 0404 agricultural biotechnology, Glucuronic Acid, alginate, Humans, Urea, Renal Insufficiency, urease, polyethyleneglycol-5000, Hexuronic Acids, Alginate, Temperature, Urea removal, 04 agricultural and veterinary sciences, Hydrogen-Ion Concentration, Enzymes, Immobilized, Urease, Microspheres, Enzyme Activation, urea removal, Polyethyleneglycol-5000

Access URL: https://pubmed.ncbi.nlm.nih.gov/17701491
https://hdl.handle.net/11454/27534
https://doi.org/10.1080/10731190701460374
https://hdl.handle.net/11454/39932
https://doi.org/10.1080/10731190701460374

Fluorinated dendritic amphiphiles, their stomatosome aggregates and application in enzyme encapsulation

Authors: Tiffany Guitton-Spassky, Florian Junge, Abhishek Kumar Singh, Boris Schade, Katharina Achazi, Marta Maglione, Stephan Sigrist, Rashmi Rashmi, Rainer Haag

Source: Nanoscale. 15:7781-7791

Subject Terms: stomatosome aggregates, Microscopy, Electron, Transmission, enzyme encapsulation, Enzymes, Immobilized, 500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften, Urease, Fluorinated dendritic amphiphiles

Access URL: https://pubmed.ncbi.nlm.nih.gov/37016756

Insulator semiconductor structures coated with biodegradable latexes as encapsulation matrix for urease

Authors: Barhoumi, H., Maaref, A., Rammah, M., Martelet, Claude, Jaffrezic-Renault, Nicole, Mousty, C., Cosnier, Serge, Perez, Emile, Rico-Lattes, Isabelle

Contributors: Ampère, Publications, Ampère (AMPERE), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de génie électrique de Lyon (CEGELY), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)

Source: Biosensors and Bioelectronics. 20:2318-2323

Subject Terms: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Latex, Biosensing Techniques, 02 engineering and technology, 01 natural sciences, GLUCOSE, PERMEATION, Coated Materials, Biocompatible, BIOSENSOR, clinical biosensor, Absorbable Implants, Materials Testing, Electrochemistry, Urea, OPTIMIZATION, urease, latex, [SPI.NRJ]Engineering Sciences [physics]/Electric power, Electric Conductivity, POLYMER, Equipment Design, Enzymes, Immobilized, HYDROLYSIS, Urease, IMMOBILIZED UREASE, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, 0104 chemical sciences, Equipment Failure Analysis, Semiconductors, [CHIM.OTHE] Chemical Sciences/Other, IS, ROTATED DISK ELECTRODE, MEMBRANE, [CHIM.OTHE]Chemical Sciences/Other, INHIBITORS, 0210 nano-technology, [SPI.NRJ] Engineering Sciences [physics]/Electric power

Access URL: https://pubmed.ncbi.nlm.nih.gov/15797333

Immobilization of urease-containing liposomes in microbeads: an approach to recover and reuse a liposomal enzyme.

Authors: Okano, Shinya¹ (AUTHOR), Yoshimoto, Makoto¹ (AUTHOR) yosimoto@yamaguchi-u.ac.jp

Source: Journal of Dispersion Science & Technology. Dec2025, p1-9. 9p. 6 Illustrations.

Subject Terms: *Urease, *Liposomes, *Molecular capsules, *Enzyme inactivation, *Immobilized enzymes, *Catalytic hydrolysis, *Microspheres

Encapsulation of Urease and Peg-Urease in Erythrocyte

Authors: Baysal, SH, Uslan, AH

Contributors: Ege University Institutional Repository

Source: Artificial Cells, Blood Substitutes, and Biotechnology. 28:263-271

Subject Terms: 0301 basic medicine, 0303 health sciences, Erythrocytes, Time Factors, Drug Compounding, Temperature, Enzymes, Immobilized, Urease, Polyethylene Glycols, 3. Good health, 03 medical and health sciences, Drug Delivery Systems, Humans, Dialysis, Cell Size

Access URL: https://www.tandfonline.com/doi/pdf/10.3109/10731190009119357?needAccess=true
https://pubmed.ncbi.nlm.nih.gov/10852677
https://hdl.handle.net/11454/36137

Urease immobilized core–shell magnetic Fe[NiFe]O4/alginate and Fe3O4/alginate composite beads with improved enzymatic stability properties: removal of artificial blood serum urea

Authors: Yasemin İspirli Doğaç, Mustafa Teke

Contributors: MÜ, Muğla Meslek Yüksekokulu, Kimya ve Kimyasal İşleme Teknolojileri Bölümü, İspirli Doğaç, Yasemin, Teke, Mustafa

Source: Journal of the Iranian Chemical Society. 18:2637-2648

Subject Terms: Urease encapsulation, 0106 biological sciences, 0301 basic medicine, 03 medical and health sciences, Fe[NiFe]O4, Alginate, Fe3O4, Nanoparticles, 01 natural sciences, Magnetic polymeric beads

File Description: application/pdf

Dual-protection mechanism in urease-immobilized calcium alginate- vaterite composite gel for enhanced Cd2+ removal from wastewater: Insights into the process

Authors: Jia, Hui ^{a, b}, Sang, Jingxuan ^a, Jin, Bingbing ^a, Wang, Shengqiang ^{a, c}, Hou, Qianqian ^a, Wu, Yifan ^a, Dapaah, Malcom Frimpong ^a, Shi, Peili ^{d, ⁎}, Cheng, Liang ^{a, b, ⁎⁎}

Source: In Process Safety and Environmental Protection December 2025 204

Interfacial crack self-healing by Sporosarcina pasteurii: From medium optimization to spore encapsulation

Authors: Chenxi Hou, Chu Wang, Ling Zheng, Jie Peng, Tao Yuan, Hui Huang, Xiaolin Lu

Source: Biointerphases. 19

Subject Terms: Spores, Bacterial, Sporosarcina, Construction Materials, Carbonates, 0211 other engineering and technologies, Urea, Chemical Precipitation, 02 engineering and technology, 0210 nano-technology, Urease, Ammonium Chloride, Culture Media

Access URL: https://pubmed.ncbi.nlm.nih.gov/39688355

Encapsulation of urease enzyme in xanthan-alginate spheres

Authors: Y M, Elçin

Source: Biomaterials. 16:1157-1161

Subject Terms: 0106 biological sciences, 0301 basic medicine, Drug Carriers, Alginates, Drug Compounding, Hexuronic Acids, Hydrolysis, Polysaccharides, Bacterial, Enzymes, Immobilized, Urease, 01 natural sciences, Hemostatics, Microspheres, 03 medical and health sciences, Glucuronic Acid, Glutaral, Microscopy, Electron, Scanning, Particle Size, Kidneys, Artificial

Access URL: https://pubmed.ncbi.nlm.nih.gov/8562792

Encapsulation of urease in double‐layered hydrogels of macroporous poly(2‐hydroxyethyl methacrylate) core and poly(ethylene oxide) outer layer: fabrication and biosensing properties

Authors: Petar Petrov, Denitza Jeleva, Christo B Tsvetanov

Source: Polymer International. 61:235-239

Subject Terms: 0301 basic medicine, 0303 health sciences, 03 medical and health sciences, 6. Clean water

Sodium alginate-based hydrogel combined with MOF-dynamic defect generation strategy: A nanobiocomposite for synergistic enhancement of urease immobilization

Authors: Wang, Zimeng ^a, Wang, Biao ^a, Yang, Leilei ^a, Guo, Xiaofang ^a, Liu, Zhuang ^a, Chai, Yamin ^b, Du, Yunzheng ^a, Tian, Ziying ^a, Yang, Jingxuan ^a, Zhu, Chunling ^a, Cao, Xuesen ^{c, ⁎}, Ou, Lailiang ^{a, ⁎}

Source: In International Journal of Biological Macromolecules January 2026 338 Part 1