Showing 1 - 20 results of 35,565 for search 'Urease biosynthesis~', query time: 1.81s Refine Results
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    Academic Journal

    Authors: Farrugia, Mark A.1, Macomber, Lee2, Hausinger, Robert P.1,2 hausinge@msu.edu

    Source: Journal of Biological Chemistry. 5/10/2013, Vol. 288 Issue 19, p13178-13185. 8p.

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    Academic Journal
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    Dissertation/ Thesis
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    Electronic Resource

    Additional Titles: We investigated the carbon dioxide metabolism of Streptococcus thermophilics, evaluating the phenotype of a phosphoenolpyruvate carboxylase-negative mutant obtained by replacement of a functional ppc gene with a deleted and inactive version, Δppc. The growth of the mutant was compared to that of the parent strain in a chemically defined medium and in milk, supplemented or not with L-aspartic acid, the final product of the metabolic pathway governed by phosphoenolpyruvate carboxylase. It was concluded that aspartate present in milk is not sufficient for the growth of S. thermophilus. As a consequence, phosphoenolpyruvate carboxylase activity was considered fundamental for the biosynthesis of L-aspartic acid in S. thermophilus metabolism. This enzymatic activity is therefore essential for growth of S. thermophilus in milk even if S. thermophilus was cultured in association with proteinase-positive Lactobacillus delbrueckii subsp. bulgaricus. It was furthermore observed that the supplementation of milk with aspartate significantly affected the level of urease activity. Further experiments, carried out with a pureI-gusA recombinant strain, revealed that expression of the urease operon was sensitive to the aspartate concentration in milk and to the cell availability of glutamate, glutamine, and ammonium ions.

    URL: https://hdl.handle.net/10281/459101
    info:eu-repo/semantics/altIdentifier/pmid/17660309
    info:eu-repo/semantics/altIdentifier/wos/WOS:000249550400012
    volume:73
    issue:18
    firstpage:5789
    lastpage:5796
    numberofpages:8
    journal:APPLIED AND ENVIRONMENTAL MICROBIOLOGY

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    Academic Journal

    Transliterated Title: Biosintez i vydelenie ureazy Staphylococcus saprophyticus L-1.

    Source: Prikladnaia biokhimiia i mikrobiologiia [Prikl Biokhim Mikrobiol] 1983 Jan-Feb; Vol. 19 (1), pp. 83-91.

    Publication Type: English Abstract; Journal Article

    Journal Info: Publisher: Izdatelstvo Nauka Country of Publication: Russia (Federation) NLM ID: 0023416 Publication Model: Print Cited Medium: Print ISSN: 0555-1099 (Print) Linking ISSN: 05551099 NLM ISO Abbreviation: Prikl Biokhim Mikrobiol Subsets: MEDLINE

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    Academic Journal

    Authors: Wang, Yi-Ning1 (AUTHOR), Li, Su-Ya1 (AUTHOR), Yuan, Liang-Chao2 (AUTHOR), Bu, Shu-Fang1 (AUTHOR), Zeng, Yao1 (AUTHOR), Xiao, Zhu-Ping1,2 (AUTHOR) xiaozhuping2005@163.com, Zhu, Hai-Liang1,2 (AUTHOR) zhuhl@nju.edu.cn

    Source: Bioorganic & Medicinal Chemistry. Mar2024, Vol. 102, pN.PAG-N.PAG. 1p.

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