Exploring Wheat Leaf Urease for a Sustainable Approach: Purification, Kinetics, and Thermal Stability Characterization

Authors: Surbhi, Sahewalla, Sonam, Sihag, Anil, Duhan, Vinod, Saharan, Ajay, Pal

Source: The Protein Journal. 44:729-741

Subject Terms: Plant Leaves, Molecular Weight, Kinetics, Enzyme Stability, Temperature, Hydrogen-Ion Concentration, Urease, Triticum, Plant Proteins

Access URL: https://pubmed.ncbi.nlm.nih.gov/40952603

Urease Inhibitor Strengthening Nano Zerovalent Iron Performance in Plant Growth Regulator-Paclobutrazol Degradation and Mitigating Its Negative Impact on Agricultural Soil Microbial Community Stability: Based on Incubation and Plant Growth Experiments

Authors: Zhou, Minzhe^aff1, Ma, Bin^aff1, Efrizal, Efrizal^{aff2, aff3}, Hayati, IsIah^aff4, Xu, Huaqin^aff1, Li, Ran^aff5, Zhao, Benliang^aff6, Tang, Wenhui^aff1, Huang, Jinpei^aff1, Wu, Huaiyu^aff1, Zhang, Manyun^{aff1, aff7}

Source: Journal of Agricultural and Food Chemistry. 73(27):16804-16821

Availability: https://pubs.acs.org/doi/10.1021/acs.jafc.5c04953

PEI-Treated Eggshell and Onion Membranes as Natural Supports for Urease Immobilization: A Performance and Stability Evaluation

Authors: Okuşluk, Abdullah Said, Kılıç, Abdulhalim

Source: ACS Omega. 10(43):51120-51131

Availability: https://pubs.acs.org/doi/10.1021/acsomega.5c05782

Molecular dynamics insights into urease stability under reservoir conditions: Implications for sustainable bio-cementation

Authors: Abdel-Azeim, Safwat

Source: In Journal of Environmental Chemical Engineering February 2026 14(1)

Influence of host cavity dimensions on the stability of cyclodextrin-based urease inhibitor formulations: a theoretical study

Authors: Freitas, Rafaela Maia Della-Sávia^Aff1, Maia, Pollyanna Pinto^Aff1, Nascimento, Clebio Soares^{Aff1, IDs00214026032959_cor1}

Source: Theoretical Chemistry Accounts: Theory, Computation, and Modeling. 145(5)

Performance and stability of urease inhibitors: Complementary effects of phenyl phosphorodiamidate and N-(n-butyl)thiophosphoric triamide under pH and temperature variations.

Authors: Jeon YJ; Department of Environmental Engineering, Chungbuk National University, 1 Chungdae-ro, Seowon-Gu, Cheongju 28644, Republic of Korea., Ahn JH; Department of Environmental Engineering, Chungbuk National University, 1 Chungdae-ro, Seowon-Gu, Cheongju 28644, Republic of Korea., Yun YM; Department of Environmental Engineering, Chungbuk National University, 1 Chungdae-ro, Seowon-Gu, Cheongju 28644, Republic of Korea. Electronic address: ymyun@chungbuk.ac.kr.

Source: Bioresource technology [Bioresour Technol] 2025 Dec; Vol. 438, pp. 133267. Date of Electronic Publication: 2025 Sep 03.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE

MeSH Terms: Urease*/antagonists & inhibitors , Urease*/metabolism , Enzyme Inhibitors*/pharmacology , Enzyme Inhibitors*/chemistry , Organothiophosphorus Compounds*/pharmacology , Temperature*, Enzyme Stability/drug effects ; Hydrogen-Ion Concentration ; Molecular Docking Simulation ; Animals ; Ammonia ; Urea ; Manure ; Organophosphorus Compounds

Experimental study on strength and water stability of loess stabilized with soybean urease and reactive MgO

Authors: Ma, Yuanbo ^a, Dong, Xuguang ^{a, b, ⁎}, Tang, Shaorong ^a, Wang, Zhen ^a, Ma, Wenguo ^a

Source: In Journal of Environmental Chemical Engineering October 2026 14(5)

Soil urease functional stability to Hg pollution: An ecotoxicological perspective

Authors: Huang, Hui, Tian, Haixia, Li, Yan, Wang, Ziquan, Yang, Tiantian, Qian, Rui, Megharaj, Mallavarapu, He, Wenxiang

Contributors: The University of Newcastle. College of Engineering, Science & Environment, School of Environmental and Life Sciences

Source: Ecotoxicology. 33:839-848

Subject Terms: urease, ecological dose, Mercury, pollution time, Ecotoxicology, Hg, Urease, Soil, Soil Pollutants, enzyme stability, resilience, Environmental Monitoring

Access URL: https://pubmed.ncbi.nlm.nih.gov/38995498

Chitosan based core–shell microgel support for urease: Step up of enzyme activity, stability and storage

Authors: Chaudhary, Aradhana ^{a, f}, Pande, P.P. ^{a, f}, Kumar, Krishna ^{b, f, ⁎}, Prasad, Tarkeshwar ^{a, f}, Rai, Shailja ^{a, f}, Singh, Vinai K. ^{c, f}, Tungala, Kranthikumar ^{d, f}, Kumar, Dhananjay ^{e, f}, Dutta, Arunava ^{e, f}

Source: In Next Materials April 2025 7

Optimization of immobilized urease enzyme on porous polymer for enhancing the stability, reusability and enzymatic kinetics using response surface methodology.

Authors: Sahin B; Department of Biotechnology, Gebze Technical University, Gebze 41400, Turkey., Ozbey-Unal B; Department of Environmental Engineering, Gebze Technical University, Gebze 41400, Turkey; Institute of Earth and Marine Sciences, Gebze Technical University, Gebze 41400, Turkey., Dizge N; Department of Environmental Engineering, Mersin University, Mersin 33343, Turkey., Keskinler B; Department of Environmental Engineering, Gebze Technical University, Gebze 41400, Turkey., Balcik C; Department of Environmental Engineering, Gebze Technical University, Gebze 41400, Turkey. Electronic address: cigdembalcik@gtu.edu.tr.

Source: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2024 Aug; Vol. 240, pp. 113986. Date of Electronic Publication: 2024 May 23.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9315133 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4367 (Electronic) Linking ISSN: 09277765 NLM ISO Abbreviation: Colloids Surf B Biointerfaces Subsets: MEDLINE

MeSH Terms: Urease*/chemistry , Urease*/metabolism , Enzymes, Immobilized*/chemistry , Enzymes, Immobilized*/metabolism , Enzyme Stability* , Surface Properties*, Polymers/chemistry ; Kinetics ; Porosity ; Hydrogen-Ion Concentration ; Temperature ; Spectroscopy, Fourier Transform Infrared ; Particle Size

Ready-to-Use or Ready-to-Adapt: Can the Self-Healing Potential of Bacillus licheniformis Be Modified?

Additional Titles: In recent years, bacteria-based self-healing has emerged as a promising bioengineering strategy to address the self-repair of cracks in cement-based materials, which represent one of the persistent durability challenges. This approach relies on microbiologically induced calcium carbonate (CaCO3) precipitation (MICP), in which metabolically active bacteria promote CaCO3 formation of crystals that can heal cracks and restore material integrity. This study compares the self-healing potential of a natural (N-) alkaline soil Bacillus licheniformis strain with a UV-strain (phenotypic mutant) generated through controlled UV exposure followed by adaptive evolution. Both strains were evaluated under conditions relevant to cementitious environments. The UV-strain exhibited enhanced ureolytic performance, reaching urease activity of 0.32 U/mg compared to 0.24 U/mg in the N-strain. This translated into improved biomineralization, with CaCO3 precipitation reaching 2.37 mg versus 2.23 mg/100 mL in the N-strain. Additionally, the UV-strain showed increased cell hydrophobicity and aggregation, indicating improved nucleation potential and surface-mediated mineral deposition. Multivariate analysis confirmed strong correlations between ureolytic metabolism, alkalization, and mineral formation, while artificial neural network (ANN) modeling (MLP 6-10-14) successfully predicted biomineralization-related parameters with high accuracy (R2 > 0.90 for urease activity, NH4+, ΔpH, and CaCO3). The results demonstrate that UV-induced phenotypic adaptation can enhance biomineralization efficiency with minor trade-offs in physiological robustness. For the first time, that controlled UV-induced phenotypic adaptation can be used as a targeted strategy to enhance biomineralization efficiency in B. licheniformis, while maintaining functional stability under cement-relevant conditions. These findings provide a novel framework for tailoring bacterial performance in self-healing systems for construction

Authors: Mejić, Luka, Šovljanski, Olja, Pezo, Milada, Pezo, Lato, Milović, Tiana, Tomić, Ana

Source: Bioengineering

Index Terms: artificial neural networks, Bacillus licheniformis, biomineralization, cementitious materials, data-driven approach, microbial self-healing concrete, microbiologically induced calcium carbonate precipitation, predictive modeling tools, UV mutagenesis, article

URL: http://vinar.vin.bg.ac.rs/bitstream/id/47957/bioengineering-13-00495.pdf
info:eu-repo/grantAgreement/MESTD/inst-2020/200134/RS
info:eu-repo/grantAgreement/MESTD/inst-2020/200156/RS
info:eu-repo/grantAgreement/MESTD/inst-2020/200051/RS
info:eu-repo/grantAgreement/MESTD/inst-2020/200017/RS

Improving the Stability of Sandy Soils by Using Urease Enzyme in Soybean Plants

Authors: Aghaalizadeh, Sepideh^{Aff1, IDs4051502400429z_cor1}, Kalantary, Farzin^Aff1, Ghanati, Faezeh^Aff2, Kahani, Mostafa^Aff1

Source: Transportation Infrastructure Geotechnology. 11(6):4275-4288

Poly(acrylamide)-co-poly(hydroxyethyl)methacrylate-co-poly(cyclohexyl methacrylate) hydrogel platform for stability, storage and biocatalytic applications of urease

Authors: Chaudhary, Aradhana ^a, Kumar, Krishna ^{a, b, ⁎}, Singh, Vinai K. ^a, Rai, Shailja ^a, Kumar, Vinod ^c, Tungala, Kranthikumar ^d, Das, Anupam ^e, Jana, Tushar ^e

Source: In International Journal of Biological Macromolecules April 2024 265 Part 2

Preparation of enzymatic microchannel reactor with immobilized urease-crosslinked enzyme aggregates and its performance

Authors: Siqi Ma, Chang Liu, Chenfeng Ma, Shuguang Wang

Source: Journal of Biotechnology. 408:142-150

Subject Terms: Bioreactors, Nitrogen, Enzyme Stability, Hydrogen-Ion Concentration, Urine, Enzymes, Immobilized, Urease

Access URL: https://pubmed.ncbi.nlm.nih.gov/40972680

Gastrointestinal stability and bioactivity of propolis from diverse origins

Authors: Can, Zehra^{Aff1, IDs0021702605104w_cor1}, Çelebi, Zeynep Berin^Aff2, Tunca, Erol^Aff3, Kolaylı, Sevgi^Aff3, Sevin, Sedat^Aff4, Tutun, Hidayet^Aff5

Source: European Food Research and Technology. 252(4)

Stability of selected nitrification and urease inhibitors in surface water

Authors: Lisa Michael, Eleonora Flores, Silke Pabst, Sondra Klitzke

Enzyme-induced carbonate precipitate (EICP) based formulation for concrete crack healing: Improvements in shelf-stability, healing effectiveness and long-term durability

Authors: Tan, Ashley C.Y., Zheng, Qiaomu ^⁎, Yang, En-Hua ^⁎

Source: In Construction and Building Materials 4 July 2026 530

Aluminum Oxide Nanoparticles‐Impregnated Aminated Silica Surfaces for Covalent Immobilization of Urease via Glutaraldehyde Activation: Stability and Kinetic Studies

Authors: Shalu Aggarwal, Saiqa Ikram

Source: ChemistrySelect. 10

Characteristics of immobilized urease onto modified zirconium (IV) oxide via glutaraldehyde: kinetic, stability, and operational stabilities in bioreactors

Authors: Alptekin, Özlem^{Aff1, IDs11696021018916_cor1}

Source: Chemical Papers. 76(2):749-761

Trichoderma inoculation alleviates Ni stress and improves yield stability of wheat (Triticum aestivum L.) through enhancing antioxidant activity, physiological response and anatomical adjustments

Authors: Bibi, Samina^Aff1, Nisa, Syeda Gul^Aff2, Mukhtar, Shiza^Aff1, Hayat, Aysha^Aff1, Quraishi, Umar Masood^{Aff1, IDs11274026050010_cor1}

Source: World Journal of Microbiology and Biotechnology. 42(6)