Molecular dynamics insights into urease stability under reservoir conditions: Implications for sustainable bio-cementation

Συγγραφείς: Abdel-Azeim, Safwat

Πηγή: In Journal of Environmental Chemical Engineering February 2026 14(1)

Optimization of immobilized urease enzyme on porous polymer for enhancing the stability, reusability and enzymatic kinetics using response surface methodology.

Συγγραφείς: Sahin B; Department of Biotechnology, Gebze Technical University, Gebze 41400, Turkey., Ozbey-Unal B; Department of Environmental Engineering, Gebze Technical University, Gebze 41400, Turkey; Institute of Earth and Marine Sciences, Gebze Technical University, Gebze 41400, Turkey., Dizge N; Department of Environmental Engineering, Mersin University, Mersin 33343, Turkey., Keskinler B; Department of Environmental Engineering, Gebze Technical University, Gebze 41400, Turkey., Balcik C; Department of Environmental Engineering, Gebze Technical University, Gebze 41400, Turkey. Electronic address: cigdembalcik@gtu.edu.tr.

Πηγή: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2024 Aug; Vol. 240, pp. 113986. Date of Electronic Publication: 2024 May 23.

Τύπος έκδοσης: Journal Article

Στοιχεία περιοδικού: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9315133 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4367 (Electronic) Linking ISSN: 09277765 NLM ISO Abbreviation: Colloids Surf B Biointerfaces Subsets: MEDLINE

Ιατρικοί όροι (MeSH): Urease*/chemistry , Urease*/metabolism , Enzymes, Immobilized*/chemistry , Enzymes, Immobilized*/metabolism , Enzyme Stability* , Surface Properties*, Polymers/chemistry ; Kinetics ; Porosity ; Hydrogen-Ion Concentration ; Temperature ; Spectroscopy, Fourier Transform Infrared ; Particle Size

Ready-to-Use or Ready-to-Adapt: Can the Self-Healing Potential of Bacillus licheniformis Be Modified?

Additional Titles: In recent years, bacteria-based self-healing has emerged as a promising bioengineering strategy to address the self-repair of cracks in cement-based materials, which represent one of the persistent durability challenges. This approach relies on microbiologically induced calcium carbonate (CaCO3) precipitation (MICP), in which metabolically active bacteria promote CaCO3 formation of crystals that can heal cracks and restore material integrity. This study compares the self-healing potential of a natural (N-) alkaline soil Bacillus licheniformis strain with a UV-strain (phenotypic mutant) generated through controlled UV exposure followed by adaptive evolution. Both strains were evaluated under conditions relevant to cementitious environments. The UV-strain exhibited enhanced ureolytic performance, reaching urease activity of 0.32 U/mg compared to 0.24 U/mg in the N-strain. This translated into improved biomineralization, with CaCO3 precipitation reaching 2.37 mg versus 2.23 mg/100 mL in the N-strain. Additionally, the UV-strain showed increased cell hydrophobicity and aggregation, indicating improved nucleation potential and surface-mediated mineral deposition. Multivariate analysis confirmed strong correlations between ureolytic metabolism, alkalization, and mineral formation, while artificial neural network (ANN) modeling (MLP 6-10-14) successfully predicted biomineralization-related parameters with high accuracy (R2 > 0.90 for urease activity, NH4+, ΔpH, and CaCO3). The results demonstrate that UV-induced phenotypic adaptation can enhance biomineralization efficiency with minor trade-offs in physiological robustness. For the first time, that controlled UV-induced phenotypic adaptation can be used as a targeted strategy to enhance biomineralization efficiency in B. licheniformis, while maintaining functional stability under cement-relevant conditions. These findings provide a novel framework for tailoring bacterial performance in self-healing systems for construction

Συγγραφείς: Mejić, Luka, Šovljanski, Olja, Pezo, Milada, Pezo, Lato, Milović, Tiana, Tomić, Ana

Πηγή: Bioengineering

Όροι ευρετηρίου: artificial neural networks, Bacillus licheniformis, biomineralization, cementitious materials, data-driven approach, microbial self-healing concrete, microbiologically induced calcium carbonate precipitation, predictive modeling tools, UV mutagenesis, article

Σύνδεσμος: http://vinar.vin.bg.ac.rs/bitstream/id/47957/bioengineering-13-00495.pdf
info:eu-repo/grantAgreement/MESTD/inst-2020/200134/RS
info:eu-repo/grantAgreement/MESTD/inst-2020/200156/RS
info:eu-repo/grantAgreement/MESTD/inst-2020/200051/RS
info:eu-repo/grantAgreement/MESTD/inst-2020/200017/RS

Urease immobilized electrospun PVA/chitosan nanofibers with improved stability and reusability characteristics: an application for removal of urea from artificial blood serum

Συγγραφείς: Kutlu, Nur, Doğaç, Yasemin İspirli, Deveci, Ilyas, Teke, Mustafa

Συνεισφορές: MÜ, Fen Fakültesi, Kimya Bölümü, MÜ, Muğla Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümü, Kutlu, Nur, Doğaç, Yasemin İspirli, Teke, Mustafa

Πηγή: Preparative Biochemistry & Biotechnology. 50:425-437

Θεματικοί όροι: Chitosan, Electrospinning, Hydrolysis, Nanofibers, Nanofiber, 02 engineering and technology, Enzymes, Immobilized, Urease, Nanocomposites, Immobilization, Kinetics, Blood Substitutes, Polyvinyl Alcohol, Urea, 0210 nano-technology

Περιγραφή αρχείου: application/pdf

Σύνδεσμος πρόσβασης: https://pubmed.ncbi.nlm.nih.gov/32233958

Synthesis and Characterisation of Biocompatible Polymer-Conjugated Magnetic Beads for Enhancement Stability of Urease

Συγγραφείς: Doğaç, Yasemin İspirli, Teke, Mustafa

Συνεισφορές: MÜ, Fen Fakültesi, Kimya Bölümü, Doğaç, Yasemin İspirli, Teke, Mustafa

Πηγή: Applied Biochemistry and Biotechnology. 179:94-110

Θεματικοί όροι: Urease Encapsulation, 0106 biological sciences, 0301 basic medicine, Chitosan, Magnetic Phenomena, Temperature, Enzymes, Immobilized, Urease, 01 natural sciences, Kinetics, 03 medical and health sciences, Biopolymers, Enzyme Stability, Fe[Nife]O-4 Nanoparticles, Urea, Fe3O4 Nanoparticles, Urease Stability, Magnetic Polymeric Beads

Περιγραφή αρχείου: application/pdf

Σύνδεσμος πρόσβασης: https://pubmed.ncbi.nlm.nih.gov/26758712

Study on the Activity and Stability of Urease Immobilized via a Saccharide-Concanavalin a Binding

Συγγραφείς: Zhou, J.-Q., Chen, S.-H., Ye, L., Li, W., Jiang, X.

Πηγή: 2008 2nd International Conference on Bioinformatics and Biomedical Engineering Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on. :990-993 May, 2008

Relation: 2008 2nd International Conference on Bioinformatics and Biomedical Engineering

PEI-Treated Eggshell and Onion Membranes as Natural Supports for Urease Immobilization: A Performance and Stability Evaluation.

Συγγραφείς: Okuşluk, Abdullah Said¹ (AUTHOR), Kılıç, Abdulhalim¹ (AUTHOR) kilicabd@itu.edu.tr

Πηγή: ACS Omega. 11/4/2025, Vol. 10 Issue 43, p51120-51131. 12p.

Molecular Dynamics Insights into Urease Stability under Reservoir Conditions: Implications for Sustainable Bio-Cementation

Συγγραφείς: Safwat Abdel-Azeim

Πηγή: Journal of Environmental Chemical Engineering. 14:121124

Reinforcement of urease through encapsulation with β-Cyclodextrin-based bioconjugates: A comparative analysis and kinetic assessment

Συγγραφείς: Rai, Shailja ^a, Pande, Poorn Prakash ^a, Kumar, Krishna ^{b, ⁎}, Tungala, Kranthikumar ^c, Tiwari, Rudramani ^d, Parwati, Km ^e, Krishnamoorthi, S. ^e, Chaudhary, Aradhana ^a, Dutta, Arunava ^f, Prasad, Tarkeshwar ^a, Singh, Vinai Kumar ^g

Πηγή: In Reactive and Functional Polymers January 2026 218

Urease Inhibitor Strengthening Nano Zerovalent Iron Performance in Plant Growth Regulator-Paclobutrazol Degradation and Mitigating Its Negative Impact on Agricultural Soil Microbial Community Stability: Based on Incubation and Plant Growth Experiments.

Συγγραφείς: Zhou, Minzhe¹ (AUTHOR), Ma, Bin¹ (AUTHOR), Efrizal, Efrizal^2,3 (AUTHOR), Hayati, IsIah⁴ (AUTHOR), Xu, Huaqin¹ (AUTHOR), Li, Ran⁵ (AUTHOR), Zhao, Benliang⁶ (AUTHOR), Tang, Wenhui¹ (AUTHOR), Huang, Jinpei¹ (AUTHOR), Wu, Huaiyu¹ (AUTHOR), Zhang, Manyun^1,7 (AUTHOR) manyunzhang@126.com

Πηγή: Journal of Agricultural & Food Chemistry. 7/9/2025, Vol. 73 Issue 27, p16804-16821. 18p.

Activity and stability of urease enzyme molecules for on-site urea measurement in milk

Additional Titles: The nitrogenous metabolite urea is an important biomarker that indicates cows’ nutritional intake. Today, determining the urea concentration from milk samples requires analysis techniques in a lab which is time-consuming and expensive. It would be favourable to have an on-site measurement method to achieve a fast detection allowing farmers to quickly adjust the feed of individual cows, which might make it possible to reduce costs, increase milk production, and/or reduce the amount of nitrogen emission to the environment. This thesis is a part of a collaboration project between Linköping University and the Swedish University of Agricultural Sciences named “On-farm measurement of milk urea - development of a sensor”. The thesis investigated the activity and stability of the urease in an electrochemical biosensor, in which the urease is immobilised via encapsulation in a gel of poly(carbamoyl sulfonate) on a screenprinted electrode coated with a metal catalyst (copper), a cation exchanger (Nafion) and a conductive polymer (polyaniline). The linear range of the biosensor was successfully extended up to 2500 μM urea with a diffusion barrier composed of chitosan and polyvinyl butyral, enabling higher urea concentrations measurement than without the barrier (680 μM). Reproducibility, reusability, and storage stability measurements of the urease immobilised electrodes were performed and evaluated. A comparison between free enzyme, immobilised enzyme on the electrode surface and immobilised enzyme on an electrode surface that had been stored for a few days, was conducted to determine urease activity and stability. In addition, five components of milk (casein, lactose, ammonium, iron, and ascorbic acid) were measured separately to evaluate their interferences during milk urea detection. Interference was observed in several cases. A final evaluation of the present electrochemical biosensor was done by analysis of real milk samples giving promising results for future developmen

Συγγραφείς: Valtersson, Emma

Όροι ευρετηρίου: Kemisk biologi, urea i mjölk, biosensor, Analytical Chemistry, Analytisk kemi, Student thesis, info:eu-repo/semantics/bachelorThesis, text

Σύνδεσμος: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-190544

Crystal engineering strategies for a healthier environment: solid-state syntheses and characterization of organic/inorganic co-crystals and host-guest systems with improved stability and performance activities

Additional Titles: In a society increasingly aware of and concerned about environmental issues, chemistry is being asked to promote sustainability through the development of processes less impacting on the environment and of products environmentally friendly. In this context, the field of chemistry referred as crystal engineering can play a major role in the quest for sustainability. By exploiting sustainable synthetic methodologies - first of all the mechanochemical method - crystal engineering can guide solid-state modifications in order to obtain crystalline materials with an improved sustainable character. This doctoral research activity addressed the synthesis and characterization of environmentally friendly crystalline materials. Two different lines of research were undertaken: • Optimization of materials of agrochemical interest, mainly through the co-crystallization method. The first systems investigated were urea-based co-crystals, designed (i) to improve the chemical-physical properties of urea by reducing its water solubility/dissolution rate and (ii) to exert an inhibition activity towards the soil enzymes urease and ammonia monooxygenase (AMO). The work resulted in the publication of three papers, which are presented in Chapter 2. A further development of this research project concerned the synthesis and characterization of crystalline materials based on AMO inhibitors, aimed to modulate the physico-chemical properties of such inhibitors in terms of water solubility, thermal stability, and inhibition activity. The experiments discussed in Chapter 3 are quite promising: a paper has been submitted upon invitation, and two more manuscripts are in preparation. • Improvement in the photostability of organic UV filters commonly used in sunscreen formulations through their inclusion into β-cyclodextrin. The inclusion complexes of β-cyclodextrin with the UV filters avobenzone and octinoxate were synthesized and proved to possess the desired properties. The results led to the publ

Συγγραφείς: Grepioni, Fabrizia, Casali, Lucia <1993>

Όροι ευρετηρίου: CHIM/03 Chimica generale e inorganica, Doctoral Thesis, PeerReviewed

Σύνδεσμος: https://amsdottorato.unibo.it/id/eprint/9804/1/PhD_Dissertation_Casali_Final.pdf
https://amsdottorato.unibo.it/id/eprint/9804/
https://amsdottorato.unibo.it/id/eprint/9804

Substitution of Asp29 with Asn29 in the metallochaperone UreE of Streptococcus thermophilus DSM 20617T increases the urease activity and anticipates urea hydrolysis during milk fermentation

Συγγραφείς: Arioli, Stefania, Mangieri, Nicola, Zanchetta, Ylenia, Russo, Pasquale, Mora, Diego ^⁎

Πηγή: In International Journal of Food Microbiology 2 May 2024 416

Activity and stability of urease-hydroxyapatite and urease-hydroxyapatite-humic acid complexes

Συγγραφείς: Claudia Borghetti, Paola Gioacchini, Claudio Marzadori, Carlo Gessa

Πηγή: Biology and Fertility of Soils. 38:96-101

Θεματικοί όροι: 0401 agriculture, forestry, and fisheries, 04 agricultural and veterinary sciences, 01 natural sciences, 0105 earth and related environmental sciences

Solid Lipid Nanoparticle Carrier Platform Containing Synthetic TLR4 Agonist Mediates Non-Viral DNA Vaccine Delivery

Additional Titles: There is a growing demand for better delivery systems to improve the stability and efficacy of DNA vaccines. Here we report the synthesis of a non-viral DNA vaccine delivery system using a novel adjuvanted solid lipid nanoparticle (SLN-A) platform as a carrier for a DNA vaccine candidate encoding the Urease alpha (UreA) antigen from Helicobacter pylori. Cationic SLN-A particles containing monophosphoryl lipid A (adjuvant) were synthesised by a modified solvent-emulsification method and were investigated for their morphology, zeta potential and in vitro transfection capacity. Particles were found to bind plasmid DNA to form lipoplexes, which were characterised by electron microscopy, dynamic light scattering and fluorescence microscopy. Cellular uptake studies confirmed particle uptake within 3 h, and intracellular localisation within endosomal compartments. In vitro studies further confirmed the ability of SLN-A particles to stimulate expression of pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α) in human macrophage-like Tohoku Hospital Pediatrics-1 (THP-1) cells. Lipoplexes were found to be biocompatible and could be efficiently transfected in murine immune cells for expression of recombinant H. pylori antigen Urease A, demonstrating their potential as a DNA vaccine delivery system

Συγγραφείς: Francis, JE, Skakic, I, Dekiwadia, C, Shukla, R, Taki, AC, Walduck, A, Smooker, PM

Όροι ευρετηρίου: Journal Article

Σύνδεσμος: http://hdl.handle.net/11343/251616

Exploring Wheat Leaf Urease for a Sustainable Approach: Purification, Kinetics, and Thermal Stability Characterization

Συγγραφείς: Sahewalla, Surbhi^{Aff1, IDs10930025102918_cor1}, Sihag, Sonam^Aff2, Duhan, Anil^Aff3, Saharan, Vinod^Aff4, Pal, Ajay^Aff1

Πηγή: The Protein Journal. 44(6):729-741

Impact of manure on soil biochemical properties: A global synthesis

Additional Titles: Manure application mitigates land degradation and improves soil fertility. Despite many individual studies on manure effects, a comprehensive overview of its consequences for a broad range of soil properties is lacking. Through a meta-analysis of 521 observations spanning the experiments from days after pulse addition up to 113 years with continues manure input, we quantified and generalized the average responses of soil biochemical properties depending on climate factors, management, soil, and manure characteristics. Large increase of pools with fast turnover (microbial carbon (C) and nitrogen (N): +88% and +84%, respectively) compared to stable organic matter pools (+27% for organic C, and +33% for total N) reflects acceleration of C and N cycles and soil fertility improvement. Activities of enzymes acquiring C-, energy-, N-, phosphorus- and sulfur were 1.3–3.3 times larger than those in soil without manure for all study durations included. Soil C/N ratio remained unaffected, indicating the stability of coupled C and N cycles. Microbial C/N ratio decreased, indicating a shift towards bacterial domination, general increase of C and N availability and acceleration of element cycling. Composted manure or manure without mineral fertilizers induced the greatest increase compared to non-composted manure or manure with mineral fertilizers, respectively, in most biochemical properties. The optimal manure application rate for adjusting proper soil pH was 25 Mg ha−1 year−1. Among manure types, swine manure caused the greatest increase of N-cycle-related properties: microbial N (+230%), urease (+258%) and N-acetyl-β-D-glucosaminidase (+138%) activities. Manure application strategies should avoid P and N losses and pollution via runoff, leaching or gaseous emissions due to fast mineralization and priming of soil organic matter. In conclusion, manure application favors C accumulation and accelerates nutrient cycling by providing available or

Συγγραφείς: Liu, S., Wang, J., Pu, S., Blagodatskaya, Evgenia, Kuzyakov, Y., Razavi, B.S.

Πηγή: ISSN: 0048-9697

Όροι ευρετηρίου: Soil nutrients, Manure, Soil organic matter, Soil leaching, Enzyme activities, Meta-analysis

Σύνδεσμος: https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23504

Influence of host cavity dimensions on the stability of cyclodextrin-based urease inhibitor formulations: a theoretical study

Συγγραφείς: Freitas, Rafaela Maia Della-Sávia^Aff1, Maia, Pollyanna Pinto^Aff1, Nascimento, Clebio Soares^{Aff1, IDs00214026032959_cor1}

Πηγή: Theoretical Chemistry Accounts: Theory, Computation, and Modeling. 145(5)

Soil urease functional stability to Hg pollution: An ecotoxicological perspective

Συγγραφείς: Huang, Hui^Aff1, Tian, Haixia^Aff1, Li, Yan^Aff1, Wang, Ziquan^Aff1, Yang, Tiantian^Aff1, Qian, Rui^{Aff1, Aff2}, Megharaj, Mallavarapu^Aff3, He, Wenxiang^{Aff1, IDs10646024027828_cor8}

Πηγή: Ecotoxicology. 33(8):839-848

Improving the Stability of Sandy Soils by Using Urease Enzyme in Soybean Plants

Συγγραφείς: Aghaalizadeh, Sepideh^{Aff1, IDs4051502400429z_cor1}, Kalantary, Farzin^Aff1, Ghanati, Faezeh^Aff2, Kahani, Mostafa^Aff1

Πηγή: Transportation Infrastructure Geotechnology. 11(6):4275-4288